Table S1 SSR markers used for the estimation of outcrossing rates, number of alleles and average polymorphic information content of six markers used for estimating outcrossing rates in twelve wild sorghum populations.

Marker / Repeat motif / primers 1 / Allele size range (bp) / Collected population / Progeny planted in field
NA2 / PIC3 / NA2 / PIC3
sb6-844 / (AG)14 / CGCTCTCGGGATGAATGA
TAACGGACCACTAACAAATGATT / 183-217 / 23 / 0.89 / 20 / 0.71
Xtxp0125 / (CT)22 / AGATCTGGCGGCAACG
AGTCACCCATCGATCATC / 161-205 / 27 / 0.92 / 25 / 0.86
Xtxp0154 / (TC)16 / CACAAACACTAGTGCCTTATC
CATAGACACCTAGGCCATC / 199-223 / 23 / 0.80 / 18 / 0.86
Xtxp0214 / (AG)18 / GAGCTGCCATAGATTTGGTCG
ACCTCGTCCCACCTTTGTTG / 169-199 / 19 / 0.82 / 21 / 0.81
Xtxp0574 / (GT)21 / GGAACTTTTGACGGGTAGTGC
CGATCGTGATGTCCCAATC / 223-257 / 21 / 0.89 / 19 / 0.90
Xtxp1414 / (GA)23 / TGTATGGCCTAGCTTATCT
CAACAAGCCAACCTAAA / 135-167 / 28 / 0.89 / 21 / 0.69
Mean / 23.5 / 0.87 / 20.7 / 0.80

1Primers: The upper sequence is the forward primer while the lower one is the reverse primer.2NA: Number of alleles per marker summed over twelve population samples consisting of 24 plants each and over their progenies consisting of 250 plants each, respectively.3PIC: Polymorphic information content averaged across the above twelve populations and across their progenies, respectively.4Brown et al (1996); 5Kong et al (2000)

Table S2: Estimates of equilibrium outcrossing rates of 62 wild sorghum populations (24 individuals per population) from Kenya using 18 and 6 (a subset of 18) SSR markers

Population / T18-SSR1 / T6-SSR2 / D3 / Population / T18-SSR1 / T6-SSR2 / D3
1 / 0.72 / 0.88 / -0.15 / 32 / 0.06 / 0.21 / -0.15
2 / 0.44 / 0.62 / -0.19 / 33 / 0.12 / 0.25 / -0.13
3 / 0.65 / 0.73 / -0.09 / 34 / 0.18 / 0.20 / -0.02
4 / 0.53 / 0.65 / -0.11 / 35 / 0.20 / 0.13 / 0.07
5 / 0.28 / 0.21 / 0.07 / 36 / 0.36 / 0.46 / -0.10
6 / 0.59 / 0.61 / -0.01 / 37 / 0.09 / 0.12 / -0.04
7 / 0.48 / 0.43 / 0.06 / 38 / 0.45 / 0.41 / 0.04
8 / 0.56 / 0.54 / 0.02 / 39 / 0.35 / 0.23 / 0.13
9 / 0.30 / 0.33 / -0.03 / 40 / 0.29 / 0.33 / -0.04
10 / 0.96 / 0.78 / 0.18 / 41 / 0.29 / 0.15 / 0.14
11 / 0.49 / 0.47 / 0.02 / 42 / 0.23 / 0.59 / -0.35
12 / 0.41 / 0.45 / -0.04 / 43 / 0.32 / 0.47 / -0.16
13 / 0.59 / 0.51 / 0.08 / 44 / 0.10 / 0.07 / 0.02
14 / 0.16 / 0.07 / 0.09 / 45 / 0.55 / 0.41 / 0.14
15 / 0.55 / 0.41 / 0.13 / 46 / 0.17 / 0.20 / -0.03
16 / 0.37 / 0.26 / 0.10 / 47 / 0.19 / 0.10 / 0.09
17 / 0.45 / 0.38 / 0.07 / 48 / 0.32 / 0.65 / -0.34
18 / 0.47 / 0.67 / -0.20 / 49 / 0.79 / 0.95 / -0.16
19 / 0.56 / 0.75 / -0.19 / 50 / 0.83 / 0.85 / -0.02
20 / 0.23 / 0.24 / -0.01 / 51 / 0.50 / 0.74 / -0.24
21 / 0.24 / 0.35 / -0.11 / 52 / 0.34 / 0.50 / -0.16
22 / 0.23 / 0.25 / -0.02 / 53 / 0.42 / 0.42 / 0.00
23 / 0.18 / 0.33 / -0.15 / 54 / 0.46 / 0.35 / 0.11
24 / 0.20 / 0.24 / -0.03 / 55 / 0.32 / 0.23 / 0.09
25 / 0.65 / 1.03 / -0.38 / 56 / 0.19 / 0.29 / -0.10
26 / 0.73 / 0.54 / 0.19 / 57 / 0.27 / 0.32 / -0.05
27 / 0.34 / 0.56 / -0.22 / 58 / 0.22 / 0.07 / 0.15
28 / 0.14 / 0.15 / -0.01 / 59 / 0.32 / 0.23 / 0.10
29 / 0.57 / 0.65 / -0.07 / 60 / 0.39 / 0.29 / 0.09
30 / 0.48 / 0.97 / -0.48 / 61 / 0.20 / 0.13 / 0.06
31 / 0.15 / 0.18 / -0.03 / 62 / 0.21 / 0.20 / 0.01

Source: Muraya et al submitted

1T18-SSR = estimates of outcrossing rate using 18 SSR markers,2T6-SSR = estimates of outcrossing rates using 6 (a subset of 18) SSR markers, and 3D = T18-SSR – T6-SSR