Table S5: Average Ct, 2-∆∆Ct and Standard error (± SE) values of cultivar C306 under different abiotic stresses. Avg. Ct values of seven unknown genes and Actin genes are mean of three independent experiments. Actin gene is used as an endogenous control.
C306 salinity
Time / Av.CtJK546455 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 26.04 / 24.55 / 1.48 / 0 / 1 / 0.039
1hr / 25.61 / 26.64 / -1.02 / -2.51 / 5.71 / 0.039
6hr / 21.88 / 24.17 / -2.2 / -3.7 / 13.6 / 0.042
24hr / 22.63 / 24.47 / -1.8 / -3.3 / 10.03 / 0.50
C306 ABA
Time / Av.CtJK546455 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 25.19 / 26.45 / -1.25 / 0 / 1 / 0.074
1hr / 27.09 / 28.90 / -1.81 / -0.55 / 1.46 / 0.029
6hr / 23.16 / 26.79 / -3.63 / -2.37 / 5.19 / 0.048
24hr / 23.90 / 28.32 / -4.41 / -3.15 / 8.89 / 0.09
C306 Cold
Time / Av.CtJK546455 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 25.79 / 26.79 / -0.99 / 0 / 1 / 0.074
1hr / 29.26 / 27.87 / 1.38 / 2.38 / 0.19 / 0.051
6hr / 26.78 / 28.11 / -1.32 / -0.33 / 1.26 / 0.029
24hr / 22.86 / 26.86 / -4.00 / -3.01 / 8.06 / 0.05
C306 water stress
Time / Av.CtJK546455 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 24.68256 / 25.47169 / -0.78913 / 0 / 1 / 0.057
1hr / 24.22965 / 24.89199 / -0.66234 / 0.126786 / 0.915869 / 0.026
6hr / 23.33946 / 24.20116 / -0.8617 / -0.07257 / 1.051587 / 0.028
24hr / 22.30601 / 22.88379 / -0.57777 / 0.211357 / 0.863724 / 0.019
C306 salinity
Time / Av.CtJK546498 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 30.16 / 28.64 / 1.48 / 0 / 1 / 0.058
1hr / 30.65 / 32.69 / -2.04 / -3.52 / 11.50 / 0.044
6hr / 30.18 / 31.42 / -1.24 / -2.72 / 6.61 / 0.076
24hr / 30.32 / 29.52 / 0.80 / -0.68 / 1.60 / 0.016
C306 ABA
Time / Av.CtJK546498 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 30.19 / 27.76 / 2.43 / 0 / 1 / 0.04
1hr / 30.27 / 30.56 / -0.28 / -2.7 / 6.56 / 0.07
6hr / 30.20 / 27.90 / 2.29 / -0.13 / 1.09 / 0.076
24hr / 30.24 / 29.54 / 0.703 / -1.72 / 3.31 / 0.02
C306 Cold
Time / Av.CtJK546498 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 30.13 / 26.25 / 3.88 / 0 / 1 / 0.019
1hr / 30.10 / 27.50 / 2.60 / -1.27 / 2.42 / 0.118
6hr / 30.37 / 27.26 / 3.11 / -0.77 / 1.70 / 0.117
24hr / 30.08 / 27.13 / 2.94 / -0.94 / 1.92 / 0.117
C306 water
Time / Av.CtJK546498 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 26.08 / 30.12 / -4.03 / 0 / 1 / 0.079
1hr / 25.13 / 30.54 / -5.40 / -1.37 / 2.58 / 0.030
6hr / 31.56 / 30.25 / 1.31 / 5.34 / 0.024 / 0.048
24hr / 34.69 / 33.26 / 1.43 / 5.47 / 0.022 / 0.030
C306 salinity
Time / Av.CtJK546482 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 33.87 / 26.58 / 7.28 / 0 / 1 / 0.136003
1hr / 34.05 / 30.35 / 3.69 / -3.58 / 12.03 / 0.016336
6hr / 33.08 / 26.74 / 6.33 / -0.94 / 1.92 / 0.049683
24hr / 33.98 / 29.45 / 4.47 / -2.80 / 7.00 / 0.038321
C306 ABA
Time / Av.CtJK546482 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 33.39 / 28.89 / 4.50 / 0 / 1 / 0.060006
1hr / 33.39 / 31.85 / 1.54 / -2.96 / 7.79 / 0.143428
6hr / 31.64 / 29.26 / 2.3 / -2.13 / 4.37 / 0.03071
24hr / 34.40 / 30.63 / 3.76 / -0.073 / 1.66 / 0.074166
C306 (Cold stress)
Time / Av.CtJK546482 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 33.78 / 27.56 / 6.22 / 0 / 1 / 0.050611
1hr / 34.02 / 29.54 / 4.48 / -1.73 / 3.33 / 0.04363
6hr / 33.40 / 28.42 / 4.98 / -1.23 / 2.35 / 0.049628
24hr / 33.57 / 28.32 / 5.24 / -0.97 / 1.97 / 0.054974
C306 (water stress)
Time / Av.CtJK546482 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 32.15 / 29.30 / 2.84 / 0 / 1 / 0.056959
1hr / 34.07 / 32.40 / 1.66 / -1.18 / 2.27 / 0.022841
6hr / 34.55 / 31.56 / 2.99 / 0.47 / 0.90 / 0.11173
24hr / 34.90 / 30.07 / 4.82 / 1.98 / 0.25 / 0.050408
C306 (salinity)
Time / Av.CtJK546505 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 30.21 / 27.79 / 2.42 / 0 / 1 / 0.207092
1hr / 33.43 / 32.49 / 0.93 / -1.48 / 2.80 / 0.085884
6hr / 31.84 / 30.58 / 1.26 / -1.16 / 2.23 / 0.148382
24hr / 30.63 / 30.35 / 0.281 / -2.14 / 4.40 / 0.257167
C306 (ABA stress)
Time / Av.CtJK546505 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 26.44 / 25.60 / 0.83 / 0 / 1 / 0.091118
1hr / 30.20 / 30.96 / -0.76 / -1.59 / 3.62 / 0.084152
6hr / 29.89 / 30.77 / -0.88 / -1.71 / 3.28 / 0.051774
24hr / 31.89 / 32.23 / -0.335 / -1.16 / 2.24 / 0.057377
C306 (Cold stress)
Time / Av.CtJK546505 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 28.46 / 27.18 / 1.27 / 0 / 1 / 0.079377
1hr / 29.69 / 29.51 / 0.18 / -1.09 / 2.13 / 0.056539
6hr / 29.86 / 30.50 / -0.64 / -1.91 / 3.77 / 0.079097
24hr / 31.54 / 32.10 / -0.50 / -1.83 / 3.57 / 0.065308
C306 (water stress)
Time / Av.CtJK546505 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 30.40 / 27.25 / 3.15 / 0 / 1 / 0.033814
1hr / 27.48 / 24.25 / 3.23 / 0.080 / 0.94 / 0.081862
6hr / 30.75 / 26.84 / 3.90 / 0.75 / 0.59 / 0.120578
24hr / 29.65 / 26.40 / 3.25 / 0.098 / 0.93 / 0.157091
C306 (salinity stress)
Time / Av.CtJK546528 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 24.04 / 23.59 / 0.44 / 0 / 1 / 0.039447
1hr / 26.61 / 27.64 / -1.02 / -1.47 / 2.78 / 0.039949
6hr / 20.88 / 23.17 / -2.28 / -2.73 / 6.6 / 0.042303
24hr / 23.96 / 27.47 / -3.50 / -3.95 / 15.5 / 0.080464
C306 (ABA stress)
Time / Av.CtJK546528 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 27.79 / 26.79 / -0.99 / 0 / 1 / 0.074
1hr / 29.26 / 30.87 / -1.61 / -0.61 / 1.53 / 0.029
6hr / 26.78 / 28.11 / -1.32 / -0.33 / 1.26 / 0.048
24hr / 22.86 / 26.86 / -4.00 / -3.01 / 8.06 / 0.090
C306 (Cold stress)
Time / Av.CtJK546528 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 24.19 / 25.45 / -1.25 / 0 / 1 / 0.074853
1hr / 26.02 / 27.63 / -1.61 / -0.35 / 1.27 / 0.087249
6hr / 22.16 / 25.79 / -3.63 / -2.37 / 5.19 / 0.029096
24hr / 22.90 / 27.45 / -4.54 / -3.28 / 9.75 / 0.055548
C306 (water stress)
Time / Av.CtJK546528 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 23.68 / 22.47 / -0.78 / 0 / 1 / 0.057719
1hr / 23.22 / 22.22 / 1.004 / 1.79 / 0.28 / 0.026103
6hr / 22.33 / 22.53 / -0.195 / 0.59 / 0.66 / 0.028631
24hr / 21.30 / 20.88 / 0.422 / 1.21 / 0.43 / 0.071337
C306 (salinity stress)
Time / Av.CtJK546542 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 30.69 / 26.48 / 4.21 / 0 / 1 / 0.014675
1hr / 30.86 / 28.83 / 2.03 / -2.18 / 4.54 / 0.095318
6hr / 30.29 / 28.14 / 2.15 / -2.06 / 4.18 / 0.0503
24hr / 31.59 / 26.52 / 5.06 / -0.84 / 0.55 / 0.049686
C306 (ABA stress)
Time / Av.CtJK546542 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 30.09 / 23.51 / 6.58 / 0 / 1 / 0.072848
1hr / 31.5 / 27.26 / 4.24 / -2.33 / 5.04 / 0.066153
6hr / 29.8 / 23.43 / 6.40 / -0.17 / 1.13 / 0.050205
24hr / 32.4 / 27.57 / 4.89 / -1.68 / 3.21 / 0.03356
C306 (Cold stress)
Time / Av.CtJK546542 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 32.20 / 25.84 / 6.36 / 0 / 1 / 0.003
1hr / 31.35 / 27.32 / 4.03 / -2.3 / 5.02 / 0.038697
6hr / 32.41 / 27.60 / 4.81 / -1.54 / 2.11 / 0
24hr / 31.52 / 27.23 / 4.29 / -2.06 / 4.19 / 0.016889
C306 (water stress)
Time / Av.CtJK546498 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 30.46 / 24.6 / 5.19 / 0 / 1 / 0.060446
1hr / 30.7 / 24.1 / 6.63 / -0.83 / 0.55 / 0.088286
6hr / 31.5 / 25.7 / 5.76 / -0.03 / 1.024 / 0.06336
24hr / 31.5 / 25.3 / 6.160 / -0.36 / 0.77 / 0.06336
C306 (salinity stress)
Time / Av.CtJK546491 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 28.63 / 26.89 / 1.74 / 0 / 1 / 0.02517
1hr / 31.29 / 31.65 / -0.35 / -2.10 / 5.20 / 0.055683
6hr / 27.81 / 27.45 / -0.36 / -1.38 / 3.76 / 0.017293
24hr / 27.52 / 28.40 / -0.850 / -2.63 / 5.29 / 0.073746
C306 (ABA stress)
Time / Av.CtJK546491 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 23.46 / 22.10 / 1.36 / 0 / 1 / 0.042095
1hr / 27.93 / 28.95 / -1.01 / -2.38 / 5.20 / 0.017074
6hr / 23.20 / 23.74 / -0.54 / -1.91 / 3.76 / 0.077116
24hr / 27.75 / 28.80 / -1.04 / -2.40 / 5.29 / 0.086366
C306 (Cold stress)
Time / Av.CtJK546491 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 23.67 / 26.29 / -2.61 / 0 / 1 / 0.039803
1hr / 26.42 / 30.33 / -3.91 / -1.29 / 2.4 / 0.045437
6hr / 23.34 / 28.55 / -5.20 / -2.58 / 6.0 / 0.043724
24hr / 24.35 / 28.58 / -4.22 / -1.60 / 3.04 / 0.047364
C306 (water stress)
Time / Av.CtJK546491 / Av.CtACTIN / ∆ Ct / ∆∆Ct / 2^-∆∆Ct / ±SE0hr / 23.12 / 24.55 / -1.43 / 0 / 1 / 0.032459
1hr / 22.96 / 23.56 / -0.60 / 0.82 / 0.56 / 0.019619
6hr / 23.04 / 24.26 / -1.222 / 0.20 / 0.86 / 0.057203
24hr / 22.5 / 23.06 / -0.53 / 0.89 / 0.53 / 0.051932