Online supplement S1: Allelic expression ratios determined in two independently generated RNA samples and the ratios obtained in three replicates of genomic DNA which was used to calibrate the experiments
RNA1a / RNA2a / Genomic DNA
Hybrid UH / Allele / Allele / Allele ratio / Hybrid UH / Allele / Allele / Allele
ratio / Hybrid UH / Allele / Allele / Allele
ratio
002x301 / 301 / 002 / 002/301 / 002x301 / 301 / 002 / 002/301 / 002x301 / 301 / 002 / 002/301
cDNA1b / 2.99 / 6.69 / 2.24 / cDNA4 / 3.20 / 5.10 / 1.59 / G1c / 6.22 / 9.01 / 1.45
cDNA2 / 3.15 / 7.82 / 2.48 / cDNA5 / 2.53 / 5.99 / 2.37 / G2 / 2.79 / 5.87 / 2.10
cDNA3 / 3.12 / 10.47 / 3.36 / cDNA6 / 3.51 / 6.57 / 1.87 / G3 / 3.32 / 6.90 / 2.08
C.V. (%) / 21.9 / 20.3 / 19.6
301x002 / 301 / 002 / 301/002 / 301x002 / 301 / 002 / 301/002 / 301x002 / 301 / 002 / 301/002
cDNA1 / 1.01 / 2.12 / 0.48 / cDNA4 / 2.05 / 2.87 / 0.71 / G1 / 8.39 / 11.94 / 0.70
cDNA2 / 2.10 / 3.83 / 0.55 / cDNA5 / 2.27 / 3.41 / 0.67 / G2 / 6.37 / 10.29 / 0.62
cDNA3 / 1.92 / 4.20 / 0.46 / cDNA6 / 3.69 / 3.54 / 1.04 / G3 / 5.16 / 6.25 / 0.83
C.V. (%) / 9.5 / 25.1 / 14.8
250x301 / 301 / 250 / 250/301 / 250x301 / 301 / 250 / 250/301 / 250x301 / 301 / 250 / 250/301
cDNA1 / 4.20 / 8.52 / 2.03 / cDNA4 / 3.74 / 4.48 / 1.20 / G1 / 6.38 / 9.03 / 1.42
cDNA2 / 5.30 / 7.67 / 1.45 / cDNA5 / 2.62 / 4.98 / 1.90 / G2 / 4.79 / 7.42 / 1.55
cDNA3 / 3.56 / 6.52 / 1.83 / cDNA6 / 1.72 / 2.58 / 1.50 / G3 / 6.38 / 9.06 / 1.42
C.V. (%) / 16.6 / 22.9 / 5.1
RNA1a / RNA2a / Genomic DNA
Hybrid UH / Allele / Allele / Allele ratio / Hybrid UH / Allele / Allele / Allele
ratio / Hybrid UH / Allele / Allele / Allele
ratio
301x250 / 301 / 250 / 301/250 / 301x250 / 301 / 250 / 301/250 / 301x250 / 301 / 250 / 301/250
cDNA1 / 2.90 / 6.86 / 0.42 / cDNA4 / 6.21 / 6.50 / 0.95 / G1 / 7.18 / 9.91 / 0.72
cDNA2 / 4.58 / 8.07 / 0.57 / cDNA5 / 0.96 / 0.99 / 0.97 / G2 / 3.78 / 8.17 / 0.46
cDNA3 / 3.33 / 8.44 / 0.40 / cDNA6 / 2.53 / 3.21 / 0.79 / G3 / 7.08 / 12.25 / 0.58
C.V. (%) / 20.0 / 10.9 / 22.2
002x250 / 002 / 250 / 002/250 / 002x250 / 002 / 250 / 002/250 / 002x250 / 002 / 250 / 002/250
cDNA1 / 7.83 / 8.64 / 0.91 / cDNA4 / 6.74 / 7.34 / 0.92 / G1 / 7.79 / 8.63 / 0.91
cDNA2 / 2.11 / 3.18 / 0.66 / cDNA5 / 6.73 / 7.86 / 0.85 / G2 / 10.86 / 11.01 / 0.99
cDNA3 / 1.05 / 1.30 / 0.81 / cDNA6 / 8.39 / 7.13 / 1.18 / G3 / 6.30 / 6.92 / 0.91
C.V. (%) / 15.9 / 17.7 / 4.9
250x002 / 002 / 250 / 250/002 / 250x002 / 002 / 250 / 250/002 / 250x002 / 002 / 250 / 250/002
cDNA1 / 7.49 / 10.33 / 1.38 / cDNA4 / 7.11 / 8.37 / 1.18 / G1 / 10.02 / 9.93 / 0.99
cDNA2 / 5.32 / 9.02 / 1.70 / cDNA5 / 7.84 / 9.06 / 1.16 / G2 / 7.15 / 8.96 / 1.25
cDNA3 / 1.25 / 2.68 / 2.14 / cDNA6 / 6.44 / 8.23 / 1.28 / G3 / 4.68 / 4.27 / 0.91
C.V. (%) / 21.9 / 5.3 / 16.9

aRNA1 and RNA2 represent two independent RNA samples that were isolated for each hybrid.

bcDNA1-3 and cDNA4-6 represent independently generated cDNA samples from RNA1 and 2, respectively.

cG1 to G3 represent three independent genomic DNA samples that were isolated for each hybrid, respectively.

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