Supplementary material 6. Fold-back structures for novel miRNA from precocious trifoliate orange.Precursor secondary structures and dG value were produced using the mfold software. rpi.edu/.miRNA and miRNA* sequences are highlighted in red and blue,respectively. The numbers along the structure are nucleotide sites from the 5’ end of the pre-miRNA sequence.
PtmiR01,scaffold_10:4756746:4756875,dG = -60.70
10 20 30 40 50 60 70
CA AAUC U -| C UCU- AUAUAACGUA A A
AAAGC GUCG UCACUCU CCCU AAGGGCUUC UCGAUCCAU CAU UGCUC A
UUUCG CAGC AGUGAGA GGGA UUCCCGAAG AGCUAGGUA GUG GUGAG A
UC GCCU U C^ - UCGC ------U
. 120 110 100 90 80
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UUAGGGCAGAGUGAUCGACU
PtmiR02,scaffold_10:4756947:4757053,dG = -52.90
10 20 30 40 50
G UAGA U UC ------| A AA AUG
CAG GUCG UCACUC CCUCAAGGGCUUC GCCCA UCCA UC C
GUC CAGC AGUGAG GGGGUUCCUGAAG UGGGU GGGU AG U
- CG-- U UU UCGUAGU^ - GG AAG
100 90 80 70 60
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:GAAGUCCUUGGGGUUGAGUGA
PtmiR03, scaffold_10:4894896:4895036,dG = -51.40
10 20 30 40
U -- A G .-UA| AA GA
UGC UAUGUU CAGA UCUGUAUGUAACA GCA UGGG \
ACG AUGCAA GUCU AGACAUACAUUGU UGU ACCU G
- CA C G \ --^ GC AC
140 130 120 50
60 70
------AGAGAGGAAA- UUG
UGCAGG AGCUG G
ACGUUU UCGAC A
CGAGUC GCGGAACAUAG UCA
110 100 90 80
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:CUGUUACAUACAGAGUCUGCA
PtmiR04, scaffold_10:5799058:5799140,dG = -21.90
10 20
C U .-UUUUUUCU| U
UG UAACU UCG U
AC AUUGA AGU U
A U \ ------^ U
.
30 40 50 60
UC- UUU U AAUAUCUGAC
UGUGA AACGAGUUAAU ACGU G
AUACU UUGCUCAAUUA UGCG C
UGU UGU - CAAUUUAAUA
100 90 80 70
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:AUUAACUCGUUUGUUCAUAUG
PtmiR05, scaffold_10:1171471:1171650,dG = -68.90
10 20 30 40 50 60 70 80
A U A G A C -- UUU---| UAG UAUAA
UAUUUAUAU GGAGUGUUUGACCAGUUUAUAUAAAGU GUUUAUAU UGGAA UAAU UGUGAUUUAA UAGUU GG G
AUAAAUAUA UCUCAUAAACUGGUCAAAUA UAUUUCA CAAGUAUA ACUUU AUUA ACACUAAGUU AUUAA CU A
C C C A A A GC UUCGGU^ UA- UGGAA
. 170 160 150 140 130 120 110 100 90
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UUGGAGUGUUUGACCAGUUUAU
PtmiR06, scaffold_10:2464703:2464899, dG = -42.00
10 20 30 40 50 60 70
G AA A C A A G .-AAAAAAAU -| AUUAA
UAAU UAUA UCAUAAAUU UU UAUAAAUUUAUUUUGUAUAAAUU AU UGGUAU UAG UCAA A
AUUA AUGU AGUGUUUAA AA AUGUUUAAAUAAAACAUGUUUAA UA ACUAUA AUC AGUU U
- CC C A C C A \ ------U^ CUCUA
190 180 170 160 150 90 80
100 110 120
UA UUUAU UU U
UAAUUUAUU UAUU GUG U
AUUAAAUAA AUAA UAC U
------CU U
140 130
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UUUGUACAAAAAUUUGUGAC
PtmiR07, scaffold_10:2585704:2585806, dG = -51.20
10 20 30 40 50
GU -| C C CC UACAG UCA G
ACC ACAGUGGUACCAUAC ACAGCU GAU AGCC UGG UCA A
UGG UGUCACCAUGGUAUG UGUCGA CUA UUGG ACC AGU A
GU A^ A C AA UA--- UA- C
100 90 80 70 60
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UCCAGCUGUAGUAUGGUACCA
PtmiR08, scaffold_10:2886633:2886728, dG = -44.90
10 20 30 40
C| G U AU A A UGUG
GA CUUG GCUGAGCAC GAUCAAUG UCAGUAAUU UCUUA \
CU GAAC UGACUCGUG UUAGUUAC AGUUAUUAA AGAAU G
A^ G U GC C - CUCG
90 80 70 60 50
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:ACCAUUGAUUCGGUGCUCAG
PtmiR09, scaffold_10:3822950:3823123, dG = -72.60
10 20 30 40 50 60 70
- GAA G UG U C- C A AU | AU C UU
GGUU AGAAAUGGGAGGAGA GAGA AG GGAAAGG GA AG GGGAGAGAAA GAGGG--UCA CU AAU C
CCAA UCUUUACCUUCCUUU CUCU UC CCUUUCC CU UC CCCUUUCUUU UUCCC AGU GA UUG A
A ACC A CA U UC U - AU \ ^ CU U UU
170 160 150 140 130 120 90 80
U--- A
GUGGA U
UAUCU C
UUAU A
110 100
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:AAUGGGAGGAGAGGAGAUGAGUG
PtmiR10, scaffold_11:1242761:1242854, dG = -62.50
10 20 30 40
GA| CCACU AA
UACGGUACCACUGUUGCAUCUAGCCGUUGGAU UGGAU A
AUGCCAUGGUGGCAACGUAGGUCGGCAACUUA AUCUA U
CC^ AAGUU GG
90 80 70 60 50
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:CAACGGCUGGAUGCAACGGUG
PtmiR12, scaffold_11:2900869:2900969, dG = -36.60
10 20 30 40 50
AACAA G-| U G UAA A G GU- G
GC UGG GGU ACGGUUCGUUAUC CA GC UG GGUGACG \
CG ACU CUA UGCUAGGCAGUGG GU CG AC CUAUUGC A
AAAUC AG^ - G UG- G A AAU U
. 90 80 70 60
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UGGUGACGGAUCGUGAUCUC
PtmiR16, scaffold_11:1213787:1213872, dG = -20.30
10
AC | G
GAGUUUA--GGUA A
UUCAGAU CCGU C
AU \ ^ U
80 20
30 40 50
UUG - GGUC U
GGGGUUUGGA CCG GUUUUAGU G
UCUUAGAUUU GGU CGAGAUUA G
G-- U A--- U
70 60
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:AGAGCAUGGUUUUAGAUUCU
PtmiR17, scaffold_11:1882319:1882457, dG = -37.40
10 20 30 40
AACUA --- C AU- UU - G- .-A| A
GUU UCU UGG GAAUA UG AAGGCA UGAUUUC AUGG U
CGA AGG ACC CUUGU AC UUCCGU ACUAAAG UACC A
GAUAA GUU U ACU UU A AG \ -^ U
130 120 110 100 50
60
------C-- AAA
GC AGUGA \
CG UCACU U
AUUCCAGGACAUAAA ACU CCA
90 80 70
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UCUGGAUGAAUAUUUGAAGGC
PtmiR18, scaffold_11:3004303:3004520, dG = -52.30
10 20 30 40 50 60 70 80 90 100
GG G AAGA U GU CUU UAUC - CAACCU| .-AAAUG - -- G - UUGAUU GACU
UU UGUAUG GAAAGUU UUGAU UGUCU UUAA UGGCCC UAAA GG AUC UAGAUGU AUACCUA CA CCU GAG U
AA ACAUAC CUUUCAG GACUA ACAGG AAUU AUCGGG AUUU CC UAG AUUUGUA UAUGGAU GU GGG CUC A
CA G GAC- U -- UGU CU-- C AAUUU-^ \ ----- U GU - A UAU--- AAAA
210 200 190 180 170 160 140 130 120 110
150
G-- AU
CUU A
GAA G
AAA AA
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UGGACAAUCAGUGACUUUCCAGC
PtmiR19, scaffold_11:4004934:4005033, dG = -48.80
10 20 30 40
-| UU U AA U - GAU
UGGUGUU AAGUGAUGCAUUUUAUGGCC AAUU AGCA UU CAA \
ACCACAA UUCACUACGUAAAAUACCGG UUAA UCGU AA GUU U
A^ C- C CC U U GUU
. 90 80 70 60
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:AGUGAUGCAUUUUAUGGCCUA
PtmiR20, scaffold_11:4857981:4858085, dG = -57.50
10 20 30 40 50
G| A AUA A C AA
UGC GGAAA CGG AAA CUUUAAAACCGCAUGGUUUUAAAGGCUUA A
ACG CCUUU GCC UUU GAAAUUUUGGUGUGCCGAAAUUUCCGAAU C
-^ C CAG C C GU
100 90 80 70 60
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UGGUUUUAAAGCUUUCCCGGA
PtmiR21, scaffold_12:775886:776101, dG = -31.50
10 20 30 40 50 60 70 80
U---- AACU C UU- U .-AUC - GACA-| CAAUC -- AAAAUAGU GAC
GACA AGUUU UUGUUGG AUUGG AG GC UACUUU UUGUA AAAAAA UAAGAAUU UCAUU \
UUGU UUAAA AAUAACU UAACC UC CG AUGAAA AAUAU UUUUUU AUUUUUAG AGUAG G
AAAUU AC-- A UAC - \ --- A AAUUC^ ----- AA GAUCUUAU AAU
210 200 190 130 120 110 100 90
140 150 160
UUAAUCUAUUAUAAAUAA UUG
GGAGAUA \
CUUUUAU U
AAUA------UAU
180 170
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:GUUUCUUGUUGGUUAUUGGUA
PtmiR22, scaffold_12:2554934:2555041, dG = -30.10
10
.-A -| U
UGAUC ACU G
ACUAG UGA A
\ - A^ U
20
30 40 50 60
G------UG A A C UU A AGG
U G GAAAUCA AUUGG AU GGUAUU CCCG G
G C CUUUAGU UAACU UG UCAUGA GGGU A
AACUAAAGAACA GU - A - UU - GUA
100 90 80 70
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:GUUCAAUAUGAUUUCCUGGAC
PtmiR23, scaffold_12:2963040:2963396, dG = -103.90
10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180
UUG CA A A C ACA UUACAAUAAU UC A AU------AA -| C UAAAAACAAAAA G U A UCUA U
ACACU UGCCAUUAUUUUUU UU UUUUUAACUUGUUUUUA AAUAUU CUU GUU UUUUUUGGAUAUUA UAAAAA UAAAUUAUCA UUAA ACU AAAAAUAAAA AAGUAUAUAUUAAUUUUUGUGGAA C CACUU UG AAAAU A
UGUGA AUGGUAAUAGAAAA AA GAAAAUUGAAUAAAAAU UUAUAA GGA UAG AAAAAAUCUGUAAU AUUUUU AUUUAAUGGU AAUU UGG UUUUUAUUUU UUUAUAUAUAAUUAAAAACACCUU G GUGAG AC UUUUA A
AAA AA C G A CG- UAAUAU---- GA A CUUUUUUU CA G^ U ------U A UCAA U
350 340 330 320 310 300 290 280 270 260 250 240 230 220 210 200 190
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin: AAAAGGAACAAAAGAUAAUGGUA
PtmiR24,scaffold_12:3298197:3298357, dG = -70.00
10 20 30 40 50 60
GAU UUU C U .-UA -| C CG
GUAUUC AUCUGCAUUUGCA CUGCAUCUUG UGCGU CUUG GUU UGGUU U
CGUAAG UAGACGUGAACGU GACGUGGAAC ACGUA GAAC CGA ACCGA A
UAC --- U U \ -- A^ A AA
. 150 140 130 70
80 90 100
AAGA- AUA A G G
GCCA GC GUCG CA A
UGGU CG CGGU GU U
UAUUG A-- A G A
120 110
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:AGGUGCAGUUGCAAGUGCAGA
PtmiR25,scaffold_12:4089434:4089628, dG = -59.20
10 20 30 40 50 60 70 80
CU GCCU G - GA .-AA GUAACAAAUUCAAAUA AUUACACACACAC
GCCAG GAGGCC UUG GGGAGAGUG CAA GUUGUCUUC UCUG A
CGGUC CUUCGG AAC CCCUCUCAC GUU UAGCGGGGG AGAC C
UC AAGU G U UC \ ------ACACACACACACA
190 180 170 160 100 90
110 120 130
AAAGAGAGA CAA| A UUAACU
GAG UGC AUUAAU \
CUC ACG UAAUUA G
A------^ G CCAAAC
150 140
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UGAGGCCGUUGGGGAGAGUGG
PtmiR26,scaffold_12:4702467:4702575, dG = -87.10
10 20 30 40 50
G| A ACUUG U
CAACGGUG UACCGUGCCACUGUUGCAUCCAGCCGUUGGAUCC AAUGGG G
GUUGCCAC AUGGCACGGUGGCAACGUGGGUUGGUAACCUAGG UUACCU G
-^ C G---- A
100 90 80 70 60
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UGGGUGCAACGGUGGCACGGU
PtmiR27,scaffold_12:1110194:1110304, dG = -57.50
10 20 30 40 50
UAA| U G U A UGUUU AA
UGC CUAAUUG AACUUUAUUGGGGAGGG UUGCUUG AAAA AGAU U
ACG GAUUAAC UUGAAAUAACCCCUCCC AACGAAC UUUU UCUG G
AAC^ U G C C CU--- AC
. 100 90 80 70 60
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:AUUGGAACUUUAUUGGGGAGG
PtmiR28,scaffold_12:1369511:1369769, dG = -56.50
10 20 30 40 50 60 70
AUCAG -- C UGU------U CAGCG .-AAGUCUA A
GAAAU AUUACUAA CUGGUC UAAUAGGU GCAAGA CAUUU GAG CAUCAC U
UUUUG UGAUGGUU GAUCGG AUUGUUCA CGUUUU GUGAA UUC GUAGUG U
UAAAA UU - UAUUGU GCUU C UUUA- \ ------G
250 240 230 220 210 200
80 90 100 110 120 130
AUCA------| C UACGAA CAAU UUAU AAACA CUU
GAAU CC CAAG AUCAA UGGAA UUGUCUUC \
CUUA GG GUUU UGGUU ACCUU AAUAGGAG U
CCCCCACCCCCCCUC^ U UAGAG- ---- UUGU AAUUC UAG
190 180 170 160 150 140
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin: AUUACUAACCUGGUCUGUUAAUA
PtmiR29,scaffold_12:2171608:2171702, dG = -60.60
10 20 30 40
UC| A UCCGA
AUCCGAAUUCAACCC AAUUUAUAACCCGAUUCGAUCCGA \
UAGGUUUAAGUUGGG UUAAAUAUUGGGCUAAGCUAGGUU A
GC^ C UAUAU
90 80 70 60 50
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin: CGGGUUAUAAAUUCGGGUUGA
PtmiR31,scaffold_12:3883408:3883531, dG = -39.20
10 20 30 40 50 60
---| U AA AG- G AC UC AA A AAAGG GA
GGG GGC UAGGAUUUAAUU CU CUG UCAUUCA CA UACUG GU GUC A
UCC UCG AUUCUAAGUUAA GA GGC AGUAAGU GU AUGAC CA UAG A
GUG^ U A- ACA G GU GA AA C ----- GU
120 110 100 90 80 70
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:AGUGAAUGAUGCGGGAGACAAAU
PtmiR32,scaffold_12:3939079:3939213, dG = -24.50
10 20 30 40
.-UGUC AAG ----| UAU C AGU
UUUUG UGUUUAAUA UUGA GGCUUUUAAAUAAGA \
AAAAU AUAAAUUGU GAUU CUGA AAGUUUAUUCU A
\ ---- GA- UAGA^ UU- A AAA
90 80 70 60 50
100 110
UAACC CGA AU
UAUAU UUGAGU \
AUAUA AAUUCA U
UUAGA UG- AA
130 120
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:AGUGUUUAAUAUUGAUAUGGCU
PtmiR33,scaffold_14:606876:607065, dG = -62.60
10 20 30 40 50 60 70 80
C U AA GAA A - U- UUC AGGUUC C .-UAG| UGA
UUC UG AUGG GAUCU GGAUAGG CGGUUCUUUAUCUU AAGUUCA ACUAU UCUUACUUG UC UGGC C
AAG AC UACU CUGGA CUUGUUC GUUAAGGAAUGGAG UUCGAGU UGAUA AGAAUGGAC AG ACCG A
C U C- AAA - G UU --- GAU--- - \ ---^ CAA
. 180 170 160 150 140 130 90
100
A--- GG GG
CC GAG G
GG CUC A
CUAA A- GG
120 110
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UGGGAAGAUCUAGGAUAGGCG
PtmiR34,scaffold_15:543493:543662, dG = -35.40
10 20 30 40 50 60 70
AAAAA------UUAA CC A UUG---- CGC .-GA| A A U GUU
UGCUC GAUGU GUGU CACAA CUUCCAC UG UUUG UG UUCA UUAUU C
AUGAG UUAUA CACG GUGUU GAAGGUG AC AAGC AC AGGU AAUAA A
AAAUAACGAAGUA ---- AU A UGUUAAA AAU \ --^ - A U AAA
. 160 150 140 130 120 90 80
100
AC----- GAUA
CCU A
GGA U
AAUACAU AAAU
110
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin: UUAAGAUGUCCGUGUACACAA
PtmiR36,scaffold_15:4289385:4289487, dG = -38.20
10 20 30 40 50
UACU| U A A U C CU CUU
UC GGA UUAAA UCCA GACGAG UUGCAAUGUUGUAAA GCG U
AG CCU AAUUU AGGU CUGCUC AAUGUUGCAAUAUUU UGU U
UUUU^ C - C U A -- CUC
100 90 80 70 60
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UAAACUCGUCUUGGACUUUAA
PtmiR38,scaffold_15:2364711:2364855, dG = -50.70
10 20 30 40 50 60 70
-| U A G GAUUAA UAAAUAUCUCUU C- UUG
CAAAUUU UUUUGUACAAACUGAU UG CAU AUUGGUUGGAU GAUCCA AUGAU U
GUUUGAA AAAACAUGUUUGACUA AC GUA UAACCAACUUA UUAGGU UAUUA U
U^ U A G AUUGAG UUU------UU UCU
140 130 120 110 100 90 80
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin: UUGUACAAACUGAUAUGGCAU
PtmiR40,scaffold_16:1771084:1771195, dG = -41.40
10 20 30 40 50
A GU--- C U C GA A---- -| A AAG
UCUGG CAUCUUCU GCGAU AG CUUCG UUG GAGGAGC CUC GU \
AGACC GUGGAAGA UGUUA UC GAAGC AAC CUUCUCG GGG CA A
- GUAGU - C - AC GGGGA U^ A AGA
110 100 90 80 70 60
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:CGAAGCUCAUUGUAGAAGGUG
PtmiR42,scaffold_17:1229280:1229494, dG = -72.40
10 20 30 40 50 60 70 80
AA C U A U-- AAAACC U U UG .-A| A A A A
AAG AAAAUCUUGC CAAGACCGCGCA UUUGU CAUGA GCAUC GGGUAU GGUU UC UGU UUUC UC GU U
UUC UUUUAGAACG GUUCUGGUGCGU GGAUA GUACU CGUAG UCCAUA CCGA AG ACG AGAG AG CA U
AG A U C UAU CGUCGU U U GU \ -^ A - A U
210 200 190 180 170 160 150 100 90
110 120 130
AGAAGAAGAAGAAGAAGAAGAAGAAUAGAAG GGA
AUU \
UAA U
G------AAA
140
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin: UCUUGCUCAAGACCGCGCAAU
PtmiR45, scaffold_18:157488:157596, dG = -55.30
10 20 30 40 50
U UA A C -| UAG
AUGUUACAUUGAAUGUACCAUACACAAU AUAA AA CACAU AAUUAUG \
UACAAUGUAACUUACAUGGUAUGUGUUG UGUU UU GUGUA UUAAUAC G
- GG C A U^ UUU
100 90 80 70 60
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UGGGUUGUGUAUGGUACAUUC
PtmiR46, scaffold_18:3903250:3903336, dG = -20.50
10 20 30 40
UU| AUC A UAU A --- GA
AUUGG CUUAAUCU AUAU UGG UCCUU AUAUGU A
UAACC GAAUUGGA UAUA GUC AGGAG UGUAUA U
AU^ C-- A CU- - UUA AA
80 70 60 50
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:AGGACUGUCAUAUAAGGUUA
PtmiR47, scaffold_19:1525:1736, dG = -69.70
10 20 30 40 50 60 70 80 90
U UG UU - G A CUU CCACU- - .-CUCUUCAU| - CG AAU CACC UCC
U GG UCAUUA CCCC UCU CCAGUCCA UAU GCAAA GCUG UCUAUUG CUG GC UGGCA UC G
G UU GGUGAU GGGG AGA GGUUAGGU AUG CGUUU CGAC AGAUAAC GGC CG ACCGU AG A
- GU UU A G - CU- CUCCCU C \ ------^ U AU AC- ACU- CUG
210 200 190 180 170 120 110 100
130 140
CCAUCAGUCGCACG A
CAUACUC \
GUAUGAG U
UCUUUACA------U
160 150
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UGGAUUGGAGAGGGGGAUAG
PtmiR48, scaffold_19:522658:522848, dG = -56.30
10 20 30 40
U UC .-CCAU C
UUUAUAUCAACUAGUCAUUUUU GUUAACU UAGC A
AAAUAUAGUUGGUUAGUGGAAA CAAUUGA AUCG C
U GA \ ---- C
. 180 170 160
50 60 70 80 90 100
UAU------AAAACUAUUU CUA| GGCCU G UA UG C
AAUUUAAAAUAA GAC UCG CCC UAG U UUUUAC U
UUAGAUUUUGUU CUG AGC GGG AUU A AAAAUG U
UAACAAUUAC ------^ AAAC- G UA GU C
150 140 130 120 110
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin: ACUAGUCAUUUUUUCGUUAA
PtmiR49, scaffold_19:651626:651754, dG = -41.40
10 20 30 40 50 60 70
UUGC| GCCAUGG C .-UUCCUUCUUCUUCUUCUCUAAUUAUA UAU
CCA CCACAG UUUCUUGAACUUCU GGCUAC U
GGU GGUGUC AAAGAACUUGAAGA UCGGUG U
CCUA^ AAAGAAG U \ ------UAG
120 110 100 80
--- U
AGC G
UCG C
CUA A
90
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UUCAAGAAAUCUGUGGGAAG
PtmiR50, scaffold_19:695753:695937, dG = -54.80
10 20 30 40 50
AUA U A C A A .-ACU| AA
UCA UA AAUC UUAUUGUCGGGGCUACGUAAU GU CAAUUAUU AGC \
GGU AU UUAG AAUAAUAGUUUCGAUGCGUUA CA GUUGAUAG UCG A
UAC U C U C C \ ---^ AA
180 170 160 150 140 60
70 80 90
UC------CAUUAAAUUUU GAA
CAUUCG UGAUCAAAAUUUU \
GUAAGU AUUAGUUUUAAAG A
GACCCAUCUCUUUAAAA ------UCG
130 120 110 100
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin: AAUCCUUAUUGUCGGGGCUAC
PtmiR51, scaffold_19:1662905:1663057, dG = -53.50
10 20 30 40 50 60
- .-AAUA| U U AUCACU GC
UGUAUAAAUUUAUUUUGUAUAAAUUGACGUGGCAU AAAUUAGU GAA UAAAU UG \
ACAUAUUUAAAUAAAACAUGUUUGACUGCACUGUA UUUAAUUA UUU AUUUA AC C
A \ ----^ U U GU---- AC
150 140 130 120 90 80 70
100
AUAUUUUU AC
AUUCA \
UAAGU C
AU------AA
110
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin: UAUUUUGUAUAAAUUGACGUG
PtmiR52, scaffold_19:3249093:3249239, dG = -28.00
10 20 30 40 50 60
AUUCACCC - A AAG G UGA .-UGAAAA| UCAU AAAU U
ACUU GAU UUUUGA UC GUUGGU ACU UUCCU UG UGCA A
UGAA CUG AGAGCU AG CAACCA UGA AAGGA AC ACGU A
CAAAUCCU C A AA- - --- \ ------^ U--- GAAU A
140 130 120 80 70
90
AA - U
CUA UUGAAU \
GGU AACUUA A
CA U C
110 100
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin: UUGAUAUUUUGAAAGUCGGUU
PtmiR54, scaffold_1:3744623:3744701, dG = -25.90
10 20 30
UUUUAA A AAA ----| GAAUCCA
GGU AGU UAC UGGAGUGGGAG C
CCA UCA AUG ACCUCACCCUC A
GUGAUA - CAA AAAA^ ACACCUC
70 60 50 40
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin: AGUAAAUACUGGAGUGGGAGG
PtmiR57, scaffold_1:12524700:12524822, dG = -36.20
10 20 30 40 50 60
G C UCA AC-| AAUUAGUUUCUU - UAG- GCU
UUACAGAAU UGUA UACA CACCCAAAA UACA CCUCU UGUU A
AAUGUCUUA ACAU AUGU GUGGGUUUU GUGU GGGGA ACAA U
- U UGA CGA^ AAU------U CUUA ACU
120 110 100 90 80 70
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UUUGGGUGAGCUGUAAGUUACA
PtmiR58, scaffold_1:13086688:13086792, dG = -37.90
10 20 30 40 50
- CU CUU-| CGAUCUA U GUUCA
AAAUUU GGAAUGGGAGG GGCAAGAAG UUU UCUU \
UUUAGG CCUUACCCUCC CCGUUCUUU AAA AGAA A
C UU CCAC^ UUAAAAG U AAAAU
100 90 80 70 60
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin: AAUGGGAGGCUUGGCAAGAAG
PtmiR59, scaffold_1:13091880:13092001, dG = -119.50
10 20 30 40 50 60
- C U U - AAAUC AUU-- C AAAG| AU
AGA AUU UCGG CAUGGGAGGA UUGGCGAGA UUUCA UUA CAU UAU \
UCU UAG AGCC GUACCCUCCU AACCGUUUU AAAGU AGU GUA GUA A
U U U - C CU--- AAAUU A GCA-^ GA
120 110 100 90 80 70
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin: GGUCAUGGGAGGAUUGGCGAGA
PtmiR61, scaffold_20:3946695:3946818, dG = -48.70
10 20 30 40 50 60
GUUC AC-| GGU C C C C C-- U
AGCU GGUGUAACUGU ACCGUGCCA AAUUG AU CAGU GUUGGAUGU AG G
UUGA CUACGUUGACA UGGCAUGGU UUAAU UA GUCG CAACUUACA UC G
CUCU ACA^ --- A A A U UAA A
120 110 100 90 80 70
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:GGUGUAACUGUGGUACCGUGCC
PtmiR62, scaffold_20:538667:539013, dG = -76.70
10 20 30 40 50 60 70 80
AUGAUAA UUC A ---- A- .-CU AG UUUC- CC---- - .-AUCUCAAAU| A
ACA GAGAUUUGGC AGAU GAC AGGUAUGU UCU UAA UCAAUCUU CU CAUA CC C
UGU CUUUAAACUG UUUG CUG UUCAUACA AGA GUU AGUUAGAA GA GUGU GG A
CAGUAAA UAU A AAAC AG \ -- CU UAACU CCUCUC C \ ------^ A
340 330 320 310 270 260 250
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UUCGAGAUUUGGCAAGAUGAC
PtmiR63, scaffold_20:905713:905867, dG = -30.30
10 20 30 40 50
U GGAGAA-- - AAU- AU .-UCA A--|UG GG
UGAA UCUAGGU UUUUUGUUG AAGA CAAAU UGUA U GUAU \
ACUU GGGUUCA AAAAACGAC UUCU GUUUG ACAU G CGUG G
U AAAUAUAG C GGUU -- \ --- AAC^GU GA
150 140 130 120 70 60
80 90
AUUAUUAGUCA ACU
UUUUAAUC G
GAAAUUGG A
UA------AUA
110 100
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin: GCAGCAAAAACACUUGGGGAU
PtmiR64, scaffold_21:1329171:1329264, dG = -33.80
10 20 30 40
U| G U - C ACG
UCUUUGUAAAGAGUGG CGU GGUC AUU UUUGUAAAGAGUGG \
AGAAAUGUUUCUUACU GUA CCAG UGA AAAUGUUUCUUACU U
-^ - U G U AGU
90 80 70 60 50
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin: AUAGUGGACCUAUGUCAUUC
PtmiR65, scaffold_21:1584153:1584254, dG = -39.20
10 20 30 40 50
GAAAUCAUCA| C UU G GUGG
AGGUAUUGGCGCG CUCAAU AAA ACGUGGUUAA G
UCUAUAACUGCGC GAGUUA UUU UGUACCGAUU C
UAUUAAUUCC^ C CU A AGUA
100 90 80 70 60
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin: AGGUAUUGGCGCGCCUCAAUU
PtmiR66, scaffold_21:419560:419771, dG = -26.50
10 20 30 40 50 60 70
CAA ------CU .-CACGUAU| U- - A UAAGUACGA
GAAAAA UAGGGAUUUGAAAAGUAAA UGAAA UAUAAA AUUA GAAUGUG CAAAA A
CUUUUU AUUUUUAAAUUUUUUAUUU AUUUU AUAUUU UGAU UUUAUAC GUUUU U
ACA UUAAUA U- \ ------^ UU A A UUACCAAUA
210 200 190 180 110 100 90 80
120 130 140
UUUUU CUAUUUAUAAAUUU AU
UUGUA AAAAUU C
AACAU UUUUGA A
----- UAAUUUACUCCUU- AC
170 160 150
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin: AGGGAUUUGAAAAGUAAACUUGA
PtmiR67, scaffold_21:1945225:1945386, dG = -47.20
10 20 30 40
G AUA-- AGCCC UG .-GC| CC
AACACAC GAGGCUUU GUGUGUU G GCUUCUG \
UUGUGUG UUUCGGAG CACACGA C CGGAGAU U
- ACAGG AUA-- GU \ --^ UU
160 150 140 130 50
60 70
.-AUAUACA A AAUG
UGUG UAU A
ACAC GUG U
\ ------A ACAA
80
90 100 110
GAUAC AC- AAA
AUAUGAU AGAUG \
UAUACUG UCUGC U
----- GUU ACA
120
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin: UGAGCACACAUAGAGGCUUUGG
PtmiR68, scaffold_22:2216907:2217018, dG = -43.20
10 20 30 40 50 60
U| U A AAAAAAAAU UU
AAUUGUUUUGCAACC UUCAAUGAAAAUUCAACG UUGUGAAAA UAUG A
UUGAUAGGAUGUUGG AAGUUAUUUUUAAGUUGU GACAUUUUU AUAU C
-^ C C ------UU
110 100 90 80 70
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin: UGAAUUUUUAUUGAACGGUUG
PtmiR69, scaffold_22:2341850:2342000, dG = -35.40
10 20 30 40 50 60
AUAA G UAAU ACU A A UA A .-UAAAA| C
AUGUC UA UUGGA CG ACAAUGGGAU CU GG UUAUCUUU GUU U
UAUAG AU GACCU GC UGUUGCCUUA GA CC AAUAGGAG CAA A
GGUC G UGGU --- - A -- - \ -----^ G
. 140 130 120 110
70 80
CUGCAA------G
AUCUU A
UAGAG A
CAAUAUUAUUUUAUUAA A
100 90
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin: UCCGUUGUCGUCCAGUGGUUA
PtmiR70, scaffold_23:541317:541402, dG = -24.90
10 20 30 40
UGGC| A UUG AC U
CAUCAUACAUA AUUGUUGUUG UAUUAUUAUUGU G
GUGGU AUGUAU UAACAAUAAU AUAAUAAUAAUA U
UAGU^ C --- A- A
80 70 60 50
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin: UACAUAUUGAUUGUUGUUGAC
PtmiR72, scaffold_24:1688008:1688109, dG = -50.80
10 20 30 40 50
UAAAA| ACA A GAAUUU
UUAUGUUGCAACUGUUGUACGGUACAACAGUU CUU CU \
GAUACAACGUUGACAAUAUGCCAUGUUGUCAA GAA GG A
AGAUA^ C-- - UCAGUA
100 90 80 70 60
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UUGCAACUGUUGUACGGUACA
PtmiR73,scaffold_24:2225994:2226146,dG = -63.80
10 20 30 40 50 60 70
U CAU U UU---- UUCAU-| UCAAAUUUAAC
UCU GUGCAGACGUUCGCAC UUUUAAAAUGCAGAU AUGUUAG GCUAAUUAGC \
AGA CACGUCUGUAGGCGUG AAAAUUUUACGUCUA UACAAUU CGAUUAAUUG A
- AAU C UACUUU GAAACU^ UACUUAUUCUU
150 140 130 120 110 100 90 80
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:AUUUUAAAACGUGCGGAUGUCU
PtmiR74,scaffold_24:403933:404065,dG = -60.80
10 20 30 40 50 60
UUGC G C A A-- AAC UU -| UUUAAA
UAGUCAGAGGC GA CUGUGAGUGGGCUAGA CG CCCAA AAAA UUG UAAU \
GUUAGUCUCCG CU GACACUCACCCGGUCU GC GGGUU UUUU AAC AUUA G
GGUU A A G CCG --- CU A^ UCUGAC
130 120 110 100 90 80 70
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:GAGGCGGACCUGUGAGUGGGC
PtmiR75,scaffold_25:1272559:1272703,dG = -40.20
10 20 30 40 50 60
UAC GA - A .-AU| CGA U A CAAUCC
GGAUUA UAUC AAUCC UAUUCGGUCC GAUUUUG AUUG AA UUUUUAAUC A
CCUAAU AUAG UUAGG GUAGGCUAGG CUAAAAC UAAC UU AAAGAUUAG A
AUA -- U C \ --^ --- - A ACACUU
140 130 120 90 80 70
100
UAAU U
ACA A
UGU U
CGUC A
110
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UGCGGAUCGGAUGCGGAUUUGAU
PtmiR77,scaffold_26:1389646:1389858,dG = -87.20
10 20 30 40 50 60 70 80 90 100
AA| U CG U CC GUCU UC CA- - C GA G GGAUU-- U AA
GUCA GAUAAAAUAAUACUACACUUC AAGU GUAUCCCAA UU AUC AUG UG GUGACAUUUAUC AUUG UGGUGU AG AG AUGU U
CAGU CUAUUUUAUUAUGAUGUGAAG UUCA CAUAGGGUU AA UAG UGC AC CACUGUAAGUAG UGAC AUCACA UU UC UACA U
CA^ - AG U AU ACU- GA AUG U A -- G AAAGUUU - GU
210 200 190 180 170 160 150 140 130 120 110
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UUGAGAAGUGUAGUAUUAUUU
PtmiR78,scaffold_26:2003929:2004039,dG = -62.90
10 20 30 40 50
UA| U AA A CCACU UU
UGGUACCGUGCC CAGUUGCAACCAGUCAUU AUCCAUUU GAU G \
AUCAUGGUACGG GUCAACGUUGGUCGGUAA UAGGUGAA CUA C A
AC^ U CC C CUCCC AC
. 100 90 80 70 60
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UGGCUGGUUGCAACUGUGGCA
PtmiR79,scaffold_26:2140455:2140580,dG = -34.00
10 20 30 40 50 60
C C A AC A C- C A C--| U AAUAAA GU
GUA AACUGU GUACUGU CACA UUGCA CAAU GUU GAU CAC UA UAAG \
UAU UUGACA CAUGGCA GUGU AACGU GUUA CAA CUA GUG AU AUUC C
C U C CA C AA A C AAA^ C AA---- AC
120 110 100 90 80 70
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UGCAACUGUGACACGGUACCA
PtmiR82,scaffold_27:2421158:2421267,dG = -47.50
10 20 30 40
G AUU C | UG
CAACU GUAUCGUGUCG AGUUGCAUUCAG--CCAUUCGA \
GUUGA CAUGGCAUAGU UCAACGUAGGUC GGUAAGUU U
- CAC A \ ^ CA
. 100 90 80 50
60
AUGGG---- U
GUC A
CGG C
AGCAAUUUA U
70
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UGGAUGCAACUAUGAUACGGU
PtmiR84,scaffold_27:1169964:1170138,dG = -47.50
10 20 30 40 50 60 70
GUUUAAAAGUA GA- A - .-A A-| C GACUCAU UUUA U
GGGAU CAAUUUGACUC AACC AAAUUUU GCCCG UUGA UC AUUU GA U
CCCUA GUUGGGCUGGG UUGG UUUAAAA CGGGC AACU AG UAAG UU U
GUUAAAUCCCA GUA A A \ - AG^ U AACUUU- CCCG U
170 160 150 140 100 90 80
110
UU--- CAAAA
GGGC \
CCCG A
CUAAC AAAUA
130 120
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:AGGGAUGACAAUUUGACUCAA
PtmiR89,scaffold_2:8403011:8403133,dG = -72.10
10 20 30 40 50 60
- AGC A -| A AA
AUACAUGAUUAUGU AGUUGUUGGAUGAAAAUCCAACG UUGUGGAAAU AUA GAU \
UAUGUACUAAUACG UCGACAACCUACUUUUAGGUUGC AACACCUUUA UGU CUA U
G GCA C U^ A AA
120 110 100 90 80 70
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:AUGUAGCAGUUGUUGGAUGAA
PtmiR92,scaffold_2:2323310:2323433,dG = -30.00
10 20 30 40 50 60
U| GU GUAUUGC U UU U CU -- UG GU-- U
CUG AA CGUCUGCGGU UUCAG AUUC AUCU GAG AAU U AUUUGUUG G
GAC UU GCGGAUGUUA AGGUU UAAG UAGG UUC UUA G UAAGCGAU A
-^ UG ------U -- U UG AU GU UUUU U
120 110 100 90 80 70
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UGGAUUGAAUUUGGAUAUUGUAG
PtmiR93,scaffold_2:3990068:3990200,dG = -49.90
10 20 30 40 50 60
AACCUUCUU ACC------| C C UAAU U A
GUCAGGU ACACCCG UUCAUCUAAAACAGGCUC CAC UCU UAAU AGA G
UAGUUCG UGUGGGC AAGUGGGUUUUGUUCGGG GUG AGA AUUA UUU U
GCUUUG--- GAACACGU A^ A C CU-- U U
130 120 110 100 90 80 70
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UGUUUUGGGUGAAACGGGUGUU
PtmiR94,scaffold_2:7132630:7132741,dG = -69.60
10 20 30 40 50
------| A AAGC U C CAC CU
GG GA UACGGU AUCACCGUUGCA CCAGCCGUUGGAUCC UGGAU A
CC CU AUGCCA UAGUGGCAACGU GGUCGGCAACCUAGG ACCUA U
CACGUUG^ A ---- - A UAC CU
110 100 90 80 70 60
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:CAACGGCUGGAUGCAACGGUG
PtmiR95,scaffold_2:7680159:7680302, dG = -33.90
10 20 30 40 50 60 70
- UUA G A CAAUUC GAA AAAUAG AA-| AA
AUAAAU UUUUGUACAAACU AUGU GCAU AUUAGUUGAAU AAUAUU UGA AUA \
UAUUUG AGAACAUGUUUGA UACA CGUA UAAUUAAUUUA UUAUAG ACU UGU U
G --- A C CUAUUU AC- AGA--- AGG^ AC
140 130 120 110 100 90 80
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UUUGUACAAACUGAUGUAGCA
PtmiR97,scaffold_2:9337953:9338039,dG = -38.60
10 20 30 40
A UC-| U CAAC UG A
CCAUACUACAGUU UUC AGCCGUUGGAU UUA AU A
GGUAUGGUGUCAA AAG UCGGCAAUCUA GAU UA C
- CGU^ - AAUC GU A
80 70 60 50
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:AUCUAACGGCUGAAUGCAAC
PtmiR100,scaffold_3:2350623:2350717,dG = -45.70
10 20 30 40
UUAUG| A AUAACAGGC
UUUAACGUAACGUACACA CACUCACAGCAACA C
AAAUUGCAUUGUAUGUGU GUGAGUGUUGUUGU C
AAGCA^ - CAUAAUUAC
90 80 70 60 50
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UGUGAGUGUGUGUAUGUUACG
PtmiR103,scaffold_3:3065858:3065929,dG = -22.80
10 20 30
A AAA----| AAG -- CAUCA
GGC UCCC GUUCGUAC UUGCAGA \
CCG AGGG CGGGUAUG AGCGUCU A
- GAACUAA^ A-- UU ACAAC
70 60 50 40
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UGCGAUUGUAUGGGCAGGGA
PtmiR108scaffold_3:179988:180099,dG = -41.20
10 20 30 40 50
- U A-| U G AAA UCCGUA
CUC UA UUA UAAAGUAAGG ACAAGUUAAAAGA AAAAAAACACAG \
GGG AU AAU GUUUCAUUCC UGUUCAAUUUUCU UUUUUUUGUGUC U
A C GA^ U - G-- UAAAGU
110 100 90 80 70 60
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UUAAAGUAAGGGACAAGUUAAA
PtmiR109,scaffold_3:1219692:1219803,dG = -24.70
10 20 30 40 50 60
-| UGUCU GUGAU U AAU A G AGACU G U
GAAU ACUGCU GA UUAUGAAUG UC CAU UC UCA AGAUUUUU U
CUUA UGACGA CU AAUGCUUAU AG GUA AG AGU UCUAAAAA G
U^ UAU-- GU--- U ------A A
110 100 90 80 70
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:CUGCUGUGAUGAUUUAUGAAUGA
PtmiR111,scaffold_3:6136921:6136998,dG = -34.00
10 20 30 40
A U UAUC--| AUUC GUC UUGC
GC UGC GGGGU GCGC GCCCAUGCG \
UG ACG CCCCA CGCG CGGGUACGU A
A - UAACCA^ ---- GUC UAUC
70 60 50
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin: GCAUGGGCCUGGCGCACCCCAC
PtmiR112,scaffold_3:6421326:6421538, dG = -110.70
10 20 30 40 50 60 70 80 90 100
AU GUU A G AA UU -----| AU U ACA A- CAAU
UCA ACCUCA GC GUU GA CCUGAAGGAAGAUGCUUCUUUGUGGUAGAUUGUUUGCUUAAUCUUUGUGAA GUUU ACC GUU GAG AAC \
GGU UGGAGU CG CAA CU GGACUUCCUUCUACGAAGAAACACCAUCUAACAAACGAAUUAGAAACACUU UAGA UGG CGA CUC UUG A
GU AUU A A GG C- AUAGU^ AU - A-- CA ACUU
210 200 190 180 170 160 150 140 130 120 110
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin:UCAGGCUCGGAACAGCAUGAG
PtmiR113,scaffold_3:8217269:8217410,dG = -42.60
10 20 30 40
A U AAGCAGC - GUU U A .-A| GC
GCA CUGC UCAAG GCA UGUA AUC GACCC CUAAU \
UGU GACG AGUUC CGU ACGU UGG UUGGG GGUUA U
- - ACAA--- G --- U G \ -^ AA
140 130 120 110
60 70 80
AAUAA AU- GA- G CG
AGCA CUGGU UUU AG \
UCGU GACCG AGA UC G
A---- CGU ACG G UU
100 90
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin: UGGGUUUGCAUGCGCUUGAA
PtmiR114,scaffold_3:9013161:9013300,dG = -26.30
10 20 30 40 50 60 70
U| C AUA UUU C AU AAU- - CCA GU- UCUAG
GGA AUUUUA GCC UUA AUUACAACG AUGG UGAAU UGU UCU GAAUUUUU \
CUU UAGGAU UGG GAU UGAUGUUGC UACC AUUUG ACG AGA UUUAAAAA A
G^ A --- CU- C C- AACC C CA- ACU UAAAC
. 130 120 110 100 90 80
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin: UCCGUUGUAGUCUAGUCGGUU
PtmiR115,scaffold_4:1239042:1239145,dG = -49.80
10 20 30 40 50
A AGCUA -| A AG UU A
CGAGAG CUGACAGAAGA GAGUGAGCAC CGC GUAA GUAUU G
GUUUUU GACUGUCUUCU CUCGCUCGUG GUG CGUU UAUAA A
- ACUGC C^ C GA UC A
100 90 80 70 60
sRNA (5' to 3' orientation) mapped to this predicted precursor hairpin: UGCUCGCUCCUCUUCUGUCAGC
PtmiR116,scaffold_4:1297202:1297333,dG = -30.30
10 20 30 40 50
A AU -- A UAA UCUU .-UCUCUCUUUC| UC