pGep-SD5 (9571 bp)

  • The pGep- retroviral cassette was from Phil Soriano (Chen, W.V. nature genetics 2004)
  • The 3’LTR of pGep- do not include the gene supF. The viral promoter and enhancer are both deleted in the U3 region of the 3’ LTR to avoid potential interference with gene expression in the resulting provirus.
  • The U3 region of 3’LTR is transfer to the 5’LTR upon provirus integration.

Plasmid Sequence:

gacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagcttgcatgcctgcaggtcccaagaagacagtaagttccaaactctgggtgaaagacccccgctgacgggtagtcaatcactcagaggagaccctcccaaggaacagcgagaccacaagtcggatgcaactgcaagagggtttattggatacacgggtacccgggcgactcagtcaatcggaggactggcgccccgagtgaggggttgtgggctcttttattgagctctagcttgccaaacctacaggtggggtctttcattcccccctttttctggagactaaataaaatcttttattttatctatggctcgtactctataggcttcagctggtgatattgttgagtcaaaactagagcctggaccactgatatcctgtctttaacaaattggactaatcgataccgtcgacTCGAGGCTAGAACTAGTGGATCCCCCGGGCTGCAGATCTGTAGGGCGCAGTAGTCCAGGGTTTCCTTGATGATGTCATACTTATCCTGTCCCTTTTTTTTCCACAGCTCGCGGTTGAGGACAAACTCTTCGCGGTCTTTCCAGTGGGGATCGACGGTATCGATAAGCTTGATGATCTGTGACATGGCGGATCCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCTTTGCCTGGTTTCCGGCACCAGAAGCGGTGCCGGAAAGCTGGCTGGAGTGCGATCTTCCTGAGGCCGATACTGTCGTCGTCCCCTCAAACTGGCAGATGCACGGTTACGATGCGCCCATCTACACCAACGTGACCTATCCCATTACGGTCAATCCGCCGTTTGTTCCCACGGAGAATCCGACGGGTTGTTACTCGCTCACATTTAATGTTGATGAAAGCTGGCTACAGGAAGGCCAGACGCGAATTATTTTTGATGGCGTTAACTCGGCGTTTCATCTGTGGTGCAACGGGCGCTGGGTCGGTTACGGCCAGGACAGTCGTTTGCCGTCTGAATTTGACCTGAGCGCATTTTTACGCGCCGGAGAAAACCGCCTCGCGGTGATGGTGCTGCGCTGGAGTGACGGCAGTTATCTGGAAGATCAGGATATGTGGCGGATGAGCGGCATTTTCCGTGACGTCTCGTTGCTGCATAAACCGACTACACAAATCAGCGATTTCCATGTTGCCACTCGCTTTAATGATGATTTCAGCCGCGCTGTACTGGAGGCTGAAGTTCAGATGTGCGGCGAGTTGCGTGACTACCTACGGGTAACAGTTTCTTTATGGCAGGGTGAAACGCAGGTCGCCAGCGGCACCGCGCCTTTCGGCGGTGAAATTATCGATGAGCGTGGTGGTTATGCCGATCGCGTCACACTACGTCTGAACGTCGAAAACCCGAAACTGTGGAGCGCCGAAATCCCGAATCTCTATCGTGCGGTGGTTGAACTGCACACCGCCGACGGCACGCTGATTGAAGCAGAAGCCTGCGATGTCGGTTTCCGCGAGGTGCGGATTGAAAATGGTCTGCTGCTGCTGAACGGCAAGCCGTTGCTGATTCGAGGCGTTAACCGTCACGAGCATCATCCTCTGCATGGTCAGGTCATGGATGAGCAGACGATGGTGCAGGATATCCTGCTGATGAAGCAGAACAACTTTAACGCCGTGCGCTGTTCGCATTATCCGAACCATCCGCTGTGGTACACGCTGTGCGACCGCTACGGCCTGTATGTGGTGGATGAAGCCAATATTGAAACCCACGGCATGGTGCCAATGAATCGTCTGACCGATGATCCGCGCTGGCTACCGGCGATGAGCGAACGCGTAACGCGAATGGTGCAGCGCGATCGTAATCACCCGAGTGTGATCATCTGGTCGCTGGGGAATGAATCAGGCCACGGCGCTAATCACGACGCGCTGTATCGCTGGATCAAATCTGTCGATCCTTCCCGCCCGGTGCAGTATGAAGGCGGCGGAGCCGACACCACGGCCACCGATATTATTTGCCCGATGTACGCGCGCGTGGATGAAGACCAGCCCTTCCCGGCTGTGCCGAAATGGTCCATCAAAAAATGGCTTTCGCTACCTGGAGAGACGCGCCCGCTGATCCTTTGCGAATACGCCCACGCGATGGGTAACAGTCTTGGCGGTTTCGCTAAATACTGGCAGGCGTTTCGTCAGTATCCCCGTTTACAGGGCGGCTTCGTCTGGGACTGGGTGGATCAGTCGCTGATTAAATATGATGAAAACGGCAACCCGTGGTCGGCTTACGGCGGTGATTTTGGCGATACGCCGAACGATCGCCAGTTCTGTATGAACGGTCTGGTCTTTGCCGACCGCACGCCGCATCCAGCGCTGACGGAAGCAAAACACCAGCAGCAGTTTTTCCAGTTCCGTTTATCCGGGCAAACCATCGAAGTGACCAGCGAATACCTGTTCCGTCATAGCGATAACGAGCTCCTGCACTGGATGGTGGCGCTGGATGGTAAGCCGCTGGCAAGCGGTGAAGTGCCTCTGGATGTCGCTCCACAAGGTAAACAGTTGATTGAACTGCCTGAACTACCGCAGCCGGAGAGCGCCGGGCAACTCTGGCTCACAGTACGCGTAGTGCAACCGAACGCGACCGCATGGTCAGAAGCCGGGCACATCAGCGCCTGGCAGCAGTGGCGTCTGGCGGAAAACCTCAGTGTGACGCTCCCCGCCGCGTCCCACGCCATCCCGCATCTGACCACCAGCGAAATGGATTTTTGCATCGAGCTGGGTAATAAGCGTTGGCAATTTAACCGCCAGTCAGGCTTTCTTTCACAGATGTGGATTGGCGATAAAAAACAACTGCTGACGCCGCTGCGCGATCAGTTCACCCGTGCACCGCTGGATAACGACATTGGCGTAAGTGAAGCGACCCGCATTGACCCTAACGCCTGGGTCGAACGCTGGAAGGCGGCGGGCCATTACCAGGCCGAAGCAGCGTTGTTGCAGTGCACGGCAGATACACTTGCTGATGCGGTGCTGATTACGACCGCTCACGCGTGGCAGCATCAGGGGAAAACCTTATTTATCAGCCGGAAAACCTACCGGATTGATGGTAGTGGTCAAATGGCGATTACCGTTGATGTTGAAGTGGCGAGCGATACACCGCATCCGGCGCGGATTGGCCTGAACTGCCAGCTGGCGCAGGTAGCAGAGCGGGTAAACTGGCTCGGATTAGGGCCGCAAGAAAACTATCCCGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAACGGTCTGCGCTGCGGGACGCGCGAATCGAATTATGGCCCACACCAGTGGCGCGGCGACTTCCAGTTCAACATCAGCCGCTACAGTCAACAGCAACTGATGGAAACCAGCCATCGCCATCTGCTGCACGCGGAAGAAGGCACATGGCTGAATATCGACGGTTTCCATATGGGGATTGGTGGCGACGACTCCTGGAGCCCGTCAGTATCGGCGGAATTCCAGCTGAGCGCCGGTCGCTACCATTACCAGTTGGTCTGGTGTCAAAAATAATAATAACCGGGCAGGGGGGATCCCCCAGATCATCGAATTCTACCGGGTAGGGGAGGCGCTTTTCCCAAGGCAGTCTGGAGCATGCGCTTTAGCAGCCCCGCTGGGCACTTGGCGCTACACAAGTGGCCTCTGGCCTCGCACACATTCCACATCCACCGGTAGGCGCCAACCGGCTCCGTTCTTTGGTGGCCCCTTCGCGCCACCTTCTACTCCTCCCCTAGTCAGGAAGTTCCCCCCCGCCCCGCAGCTCGCGTCGTGCAGGACGTGACAAATGGAAGTAGCACGTCTCACTAGTCTCGTGCAGATGGACAGCACCGCTGAGCAATGGAAGCGGGTAGGCCTTTGGGGCAGCGGCCAATAGCAGCTTTGCTCCTTCGCTTTCTGGGCTCAGAGGCTGGGAAGGGGTGGGTCCGGGGGCGGGCTCAGGGGCGGGCTCAGGGGCGGGGCGGGCGCCCGAAGGTCCTCCGGAGGCCCGGCATTCTGCACGCTTCAAAAGCGCACGTCTGCCGCGCTGTTCTCCTCTTCCTCATCTCCGGGCCTTTCGACCTGCAGCCAATATGGGATCGGCCATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGATGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGGGGATCAATTCTCTAGCGCGCCTGAGTACTTCAGGGATTTGAATGTAAGTAATGCTTTTTTTTTTCTCGCTCTCGTTTTTCAAAAACCCACATAAAAATTGAGGAAAGGGAAGAATTGTTTTCTCCTTCCAGCACCTCGTAATTTGACCCGACTGATGGTTCCCATTAGTCACATAAAGCTGTAGTCAAGTACAGACGTCCTTAGAACTGGAACTTGGCCAGGCTACGGTGACACTTCTTGCTGGCTGAAATAGTTGAACAGCTTTAATATAGGCGCGCTAGAGTCGACCTCGAgaaggagtgagggctggataaagggaggatcgaggcggggtcgaacgaggaggttcaagggggagagacggggcggatggaggaagaggaggcggaggcttagggtgtacaaagggcttgacccagggaggggggtcaaaagccaaggcttcccaggtcacgatgtaggggacctggtctgggtgtccatgcgggccaggtgaaaagaccttgatcttaacctgggtgatgaggtctcggttaaaggtgccgtctcgcggccatccgacgttaaaggttggccattctgcagagcagaaggtaacccaacgtctcttcttgacatctaccgactggttgtgagcgatccgctcgacatctttccagtgacctaaggtcaaacttaagggagtggtaacagtctggcccatattctcagacaaatacagaaacacagtcagacagagacaacacagaacgatgctgcagcagacaagacgcgcggcgcggcttcggtcccaaaccgaaagcaaaaattcagacggaggcgggaactgttttaggttctcgtctcctaccagaaccacatatccctcctctaaggggggtgcaccaaagagtccaaaacgatcgggatttttggactcaggtcgggccacaaaaacggcccccgaagtccctgggacgtctcccagggttgcggccgggtgttccgaactcgtcagttccaccacgggtccgccagatacagagctagttagctaactagtaccgacgcaggcgcataaaatcagtcatagacactagacaatcggacagacacagataagttgctggccagcttacctcccggtggtgggtcggtggtccctgggcaggggtctcccgatcccggacgagcccccaaatgaaagacccccgctgacgggtagtcaatcactcagaggagaccctcccaaggaacagcgagaccacaagtcggatgcaactgcaagagggtttattggatacacgggtacccgggcgactcagtcaatcggaggactggcgccccgagtgaggggttgtgggctcttttattgagctcggggagcagaagcgcgcgaacagaagcgagaagcgaactgattggttagttcaaataaggcacagggtcatttcaggtccttggggcaccctggaaacatctgatggttctctagaaactgctgagggctggaccgcatctggggaccatctgttcttggccctgagccggggcaggaactgcttaccacagatatcctgtttggcccatattcagctgttccatctgttcttggccctgagccggggcaggaactgcttaccacagatatcctgtttggcccatattcagctgttccatctgttcctgaccttgatctgaacttctctattctcagttatgtatttttccatgccttgcaaaatggcgttacttaagctagcttgccaaacctacaggtggggtctttcattcccccctttttctggagactaaataaaatcttttattttatctatggctcgtactctataggcttcagctggtgatattgttgagtcaaaactagagcctggaccactgatatcctgtctttaacaaattggactaatcgctcgaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaat4ggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcga

Gep-SD5 (6368bp) Proviral Sequence:

tgaaagacccccgctgacgggtagtcaatcactcagaggagaccctcccaaggaacagcgagaccacaagtcggatgcaactgcaagagggtttattggatacacgggtacccgggcgactcagtcaatcggaggactggcgccccgagtgaggggttgtgggctcttttattgagcttaagctagcttgccaaacctacaggtggggtctttcattcccccctttttctggagactaaataaaatcttttattttatctatggctcgtactctataggcttcagctggtgatattgttgagtcaaaactagagcctggaccactgatatcctgtctttaacaaattggactaatcgataccgtcgacTCGAGGCTAGAACTAGTGGATCCCCCGGGCTGCAGATCTGTAGGGCGCAGTAGTCCAGGGTTTCCTTGATGATGTCATACTTATCCTGTCCCTTTTTTTTCCACAGCTCGCGGTTGAGGACAAACTCTTCGCGGTCTTTCCAGTGGGGATCGACGGTATCGATAAGCTTGATGATCTGTGACATGGCGGATCCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCTTTGCCTGGTTTCCGGCACCAGAAGCGGTGCCGGAAAGCTGGCTGGAGTGCGATCTTCCTGAGGCCGATACTGTCGTCGTCCCCTCAAACTGGCAGATGCACGGTTACGATGCGCCCATCTACACCAACGTGACCTATCCCATTACGGTCAATCCGCCGTTTGTTCCCACGGAGAATCCGACGGGTTGTTACTCGCTCACATTTAATGTTGATGAAAGCTGGCTACAGGAAGGCCAGACGCGAATTATTTTTGATGGCGTTAACTCGGCGTTTCATCTGTGGTGCAACGGGCGCTGGGTCGGTTACGGCCAGGACAGTCGTTTGCCGTCTGAATTTGACCTGAGCGCATTTTTACGCGCCGGAGAAAACCGCCTCGCGGTGATGGTGCTGCGCTGGAGTGACGGCAGTTATCTGGAAGATCAGGATATGTGGCGGATGAGCGGCATTTTCCGTGACGTCTCGTTGCTGCATAAACCGACTACACAAATCAGCGATTTCCATGTTGCCACTCGCTTTAATGATGATTTCAGCCGCGCTGTACTGGAGGCTGAAGTTCAGATGTGCGGCGAGTTGCGTGACTACCTACGGGTAACAGTTTCTTTATGGCAGGGTGAAACGCAGGTCGCCAGCGGCACCGCGCCTTTCGGCGGTGAAATTATCGATGAGCGTGGTGGTTATGCCGATCGCGTCACACTACGTCTGAACGTCGAAAACCCGAAACTGTGGAGCGCCGAAATCCCGAATCTCTATCGTGCGGTGGTTGAACTGCACACCGCCGACGGCACGCTGATTGAAGCAGAAGCCTGCGATGTCGGTTTCCGCGAGGTGCGGATTGAAAATGGTCTGCTGCTGCTGAACGGCAAGCCGTTGCTGATTCGAGGCGTTAACCGTCACGAGCATCATCCTCTGCATGGTCAGGTCATGGATGAGCAGACGATGGTGCAGGATATCCTGCTGATGAAGCAGAACAACTTTAACGCCGTGCGCTGTTCGCATTATCCGAACCATCCGCTGTGGTACACGCTGTGCGACCGCTACGGCCTGTATGTGGTGGATGAAGCCAATATTGAAACCCACGGCATGGTGCCAATGAATCGTCTGACCGATGATCCGCGCTGGCTACCGGCGATGAGCGAACGCGTAACGCGAATGGTGCAGCGCGATCGTAATCACCCGAGTGTGATCATCTGGTCGCTGGGGAATGAATCAGGCCACGGCGCTAATCACGACGCGCTGTATCGCTGGATCAAATCTGTCGATCCTTCCCGCCCGGTGCAGTATGAAGGCGGCGGAGCCGACACCACGGCCACCGATATTATTTGCCCGATGTACGCGCGCGTGGATGAAGACCAGCCCTTCCCGGCTGTGCCGAAATGGTCCATCAAAAAATGGCTTTCGCTACCTGGAGAGACGCGCCCGCTGATCCTTTGCGAATACGCCCACGCGATGGGTAACAGTCTTGGCGGTTTCGCTAAATACTGGCAGGCGTTTCGTCAGTATCCCCGTTTACAGGGCGGCTTCGTCTGGGACTGGGTGGATCAGTCGCTGATTAAATATGATGAAAACGGCAACCCGTGGTCGGCTTACGGCGGTGATTTTGGCGATACGCCGAACGATCGCCAGTTCTGTATGAACGGTCTGGTCTTTGCCGACCGCACGCCGCATCCAGCGCTGACGGAAGCAAAACACCAGCAGCAGTTTTTCCAGTTCCGTTTATCCGGGCAAACCATCGAAGTGACCAGCGAATACCTGTTCCGTCATAGCGATAACGAGCTCCTGCACTGGATGGTGGCGCTGGATGGTAAGCCGCTGGCAAGCGGTGAAGTGCCTCTGGATGTCGCTCCACAAGGTAAACAGTTGATTGAACTGCCTGAACTACCGCAGCCGGAGAGCGCCGGGCAACTCTGGCTCACAGTACGCGTAGTGCAACCGAACGCGACCGCATGGTCAGAAGCCGGGCACATCAGCGCCTGGCAGCAGTGGCGTCTGGCGGAAAACCTCAGTGTGACGCTCCCCGCCGCGTCCCACGCCATCCCGCATCTGACCACCAGCGAAATGGATTTTTGCATCGAGCTGGGTAATAAGCGTTGGCAATTTAACCGCCAGTCAGGCTTTCTTTCACAGATGTGGATTGGCGATAAAAAACAACTGCTGACGCCGCTGCGCGATCAGTTCACCCGTGCACCGCTGGATAACGACATTGGCGTAAGTGAAGCGACCCGCATTGACCCTAACGCCTGGGTCGAACGCTGGAAGGCGGCGGGCCATTACCAGGCCGAAGCAGCGTTGTTGCAGTGCACGGCAGATACACTTGCTGATGCGGTGCTGATTACGACCGCTCACGCGTGGCAGCATCAGGGGAAAACCTTATTTATCAGCCGGAAAACCTACCGGATTGATGGTAGTGGTCAAATGGCGATTACCGTTGATGTTGAAGTGGCGAGCGATACACCGCATCCGGCGCGGATTGGCCTGAACTGCCAGCTGGCGCAGGTAGCAGAGCGGGTAAACTGGCTCGGATTAGGGCCGCAAGAAAACTATCCCGACCGCCTTACTGCCGCCTGTTTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAACGGTCTGCGCTGCGGGACGCGCGAATCGAATTATGGCCCACACCAGTGGCGCGGCGACTTCCAGTTCAACATCAGCCGCTACAGTCAACAGCAACTGATGGAAACCAGCCATCGCCATCTGCTGCACGCGGAAGAAGGCACATGGCTGAATATCGACGGTTTCCATATGGGGATTGGTGGCGACGACTCCTGGAGCCCGTCAGTATCGGCGGAATTCCAGCTGAGCGCCGGTCGCTACCATTACCAGTTGGTCTGGTGTCAAAAATAATAATAACCGGGCAGGGGGGATCCCCCAGATCATCGAATTCTACCGGGTAGGGGAGGCGCTTTTCCCAAGGCAGTCTGGAGCATGCGCTTTAGCAGCCCCGCTGGGCACTTGGCGCTACACAAGTGGCCTCTGGCCTCGCACACATTCCACATCCACCGGTAGGCGCCAACCGGCTCCGTTCTTTGGTGGCCCCTTCGCGCCACCTTCTACTCCTCCCCTAGTCAGGAAGTTCCCCCCCGCCCCGCAGCTCGCGTCGTGCAGGACGTGACAAATGGAAGTAGCACGTCTCACTAGTCTCGTGCAGATGGACAGCACCGCTGAGCAATGGAAGCGGGTAGGCCTTTGGGGCAGCGGCCAATAGCAGCTTTGCTCCTTCGCTTTCTGGGCTCAGAGGCTGGGAAGGGGTGGGTCCGGGGGCGGGCTCAGGGGCGGGCTCAGGGGCGGGGCGGGCGCCCGAAGGTCCTCCGGAGGCCCGGCATTCTGCACGCTTCAAAAGCGCACGTCTGCCGCGCTGTTCTCCTCTTCCTCATCTCCGGGCCTTTCGACCTGCAGCCAATATGGGATCGGCCATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGATGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGGGGATCAATTCTCTAGCGCGCCTGAGTACTTCAGGGATTTGAATGTAAGTAATGCTTTTTTTTTTCTCGCTCTCGTTTTTCAAAAACCCACATAAAAATTGAGGAAAGGGAAGAATTGTTTTCTCCTTCCAGCACCTCGTAATTTGACCCGACTGATGGTTCCCATTAGTCACATAAAGCTGTAGTCAAGTACAGACGTCCTTAGAACTGGAACTTGGCCAGGCTACGGTGACACTTCTTGCTGGCTGAAATAGTTGAACAGCTTTAATATAGGCGCGCTAGAGTCGACCTCGAgaaggagtgagggctggataaagggaggatcgaggcggggtcgaacgaggaggttcaagggggagagacggggcggatggaggaagaggaggcggaggcttagggtgtacaaagggcttgacccagggaggggggtcaaaagccaaggcttcccaggtcacgatgtaggggacctggtctgggtgtccatgcgggccaggtgaaaagaccttgatcttaacctgggtgatgaggtctcggttaaaggtgccgtctcgcggccatccgacgttaaaggttggccattctgcagagcagaaggtaacccaacgtctcttcttgacatctaccgactggttgtgagcgatccgctcgacatctttccagtgacctaaggtcaaacttaagggagtggtaacagtctggcccatattctcagacaaatacagaaacacagtcagacagagacaacacagaacgatgctgcagcagacaagacgcgcggcgcggcttcggtcccaaaccgaaagcaaaaattcagacggaggcgggaactgttttaggttctcgtctcctaccagaaccacatatccctcctctaaggggggtgcaccaaagagtccaaaacgatcgggatttttggactcaggtcgggccacaaaaacggcccccgaagtccctgggacgtctcccagggttgcggccgggtgttccgaactcgtcagttccaccacgggtccgccagatacagagctagttagctaactagtaccgacgcaggcgcataaaatcagtcatagacactagacaatcggacagacacagataagttgctggccagcttacctcccggtggtgggtcggtggtccctgggcaggggtctcccgatcccggacgagcccccaaatgaaagacccccgctgacgggtagtcaatcactcagaggagaccctcccaaggaacagcgagaccacaagtcggatgcaactgcaagagggtttattggatacacgggtacccgggcgactcagtcaatcggaggactggcgccccgagtgaggggttgtgggctcttttattgagcttaagctagcttgccaaacctacaggtggggtctttca

Restriction Enzyme Map

Restriction Enzyme Map:

3’LTR

1 TGAAAGACCCCCGCTGACGGGTAGTCAATCACTCAGAGGAGACCCTCCCAAGGAACAGCGAGACCACAAGTCGGATGCAA 80

1 ACTTTCTGGGGGCGACTGCCCATCAGTTAGTGAGTCTCCTCTGGGAGGGTTCCTTGTCGCTCTGGTGTTCAGCCTACGTT 80

DdeI

81 CTGCAAGAGGGTTTATTGGATACACGGGTACCCGGGCGACTCAGTCAATCGGAGGACTGGCGCCCCGAGTGAGGGGTTGT 160

81 GACGTTCTCCCAAATAACCTATGTGCCCATGGGCCCGCTGAGTCAGTTAGCCTCCTGACCGCGGGGCTCACTCCCCAACA 160

RsaI HinfI NarI

KpnI DdeI

NciI

MspI

SmaI

NciI

161 GGGCTCTTTTATTGAGCTTAAGCTAGCTTGCCAAACCTACAGGTGGGGTCTTTCATTCCCCCCTTTTTCTGGAGACTAAA 240

161 CCCGAGAAAATAACTCGAATTCGATCGAACGGTTTGGATGTCCACCCCAGAAAGTAAGGGGGGAAAAAGACCTCTGATTT 240

AluI AluI

NheI

AluI

241 TAAAATCTTTTATTTTATCTATGGCTCGTACTCTATAGGCTTCAGCTGGTGATATTGTTGAGTCAAAACTAGAGCCTGGA 320

241 ATTTTAGAAAATAAAATAGATACCGAGCATGAGATATCCGAAGTCGACCACTATAACAACTCAGTTTTGATCTCGGACCT 320

RsaI PvuII HinfI EcoRII

AluI

321 CCACTGATATCCTGTCTTTAACAAATTGGACTAATCGATACCGTCGACTCGAGGCTAGAACTAGTGGATCCCCCGGGCTG 400

321 GGTGACTATAGGACAGAAATTGTTTAACCTGATTAGCTATGGCAGCTGAGCTCCGATCTTGATCACCTAGGGGGCCCGAC 400

EcoRV TaqI SalI TaqI SpeI BamHI NciI

TaqI Sau3AI SmaI

HincII DpnI NciI

HinfI MspI

XhoI

Human Adenovirus Tupe 3/5 SA splice site

401 CAGATCTGTAGGGCGCAGTAGTCCAGGGTTTCCTTGATGATGTCATACTTATCCTGTCCCTTTTTTTTCCACAGCTCGCG 480

401 GTCTAGACATCCCGCGTCATCAGGTCCCAAAGGAACTACTACAGTATGAATAGGACAGGGAAAAAAAAGGTGTCGAGCGC 480

PstI EcoRII AluI

BglII

Sau3AI

DpnI

-galactosidase

481 GTTGAGGACAAACTCTTCGCGGTCTTTCCAGTGGGGATCGACGGTATCGATAAGCTTGATGATCTGTGACATGGCGGATC 560

481 CAACTCCTGTTTGAGAAGCGCCAGAAAGGTCACCCCTAGCTGCCATAGCTATTCGAACTACTAGACACTGTACCGCCTAG 560

Sau3AI TaqI AluI Sau3AI BamHI

DpnI HindIII DpnI Sau3AI

TaqI DpnI

561 CCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCC 640

561 GGCAGCAAAATGTTGCAGCACTGACCCTTTTGGGACCGCAATGGGTTGAATTAGCGGAACGTCGTGTAGGGGGAAAGCGG 640

EcoRII

641 AGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCTTTGC 720

641 TCGACCGCATTATCGCTTCTCCGGGCGTGGCTAGCGGGAAGGGTTGTCAACGCGTCGGACTTACCGCTTACCGCGAAACG 720

PvuII Sau3AI BglI EcoRII

AluI DpnI

PvuI

721 CTGGTTTCCGGCACCAGAAGCGGTGCCGGAAAGCTGGCTGGAGTGCGATCTTCCTGAGGCCGATACTGTCGTCGTCCCCT 800

721 GACCAAAGGCCGTGGTCTTCGCCACGGCCTTTCGACCGACCTCACGCTAGAAGGACTCCGGCTATGACAGCAGCAGGGGA 800

MspI MspI AluI Sau3AI DdeI

DpnI

801 CAAACTGGCAGATGCACGGTTACGATGCGCCCATCTACACCAACGTGACCTATCCCATTACGGTCAATCCGCCGTTTGTT 880

801 GTTTGACCGTCTACGTGCCAATGCTACGCGGGTAGATGTGGTTGCACTGGATAGGGTAATGCCAGTTAGGCGGCAAACAA 880

881 CCCACGGAGAATCCGACGGGTTGTTACTCGCTCACATTTAATGTTGATGAAAGCTGGCTACAGGAAGGCCAGACGCGAAT 960

881 GGGTGCCTCTTAGGCTGCCCAACAATGAGCGAGTGTAAATTACAACTACTTTCGACCGATGTCCTTCCGGTCTGCGCTTA 960

HinfI AluI

961 TATTTTTGATGGCGTTAACTCGGCGTTTCATCTGTGGTGCAACGGGCGCTGGGTCGGTTACGGCCAGGACAGTCGTTTGC 1040

961 ATAAAAACTACCGCAATTGAGCCGCAAAGTAGACACCACGTTGCCCGCGACCCAGCCAATGCCGGTCCTGTCAGCAAACG 1040

HincII EcoRII

HpaI

1041 CGTCTGAATTTGACCTGAGCGCATTTTTACGCGCCGGAGAAAACCGCCTCGCGGTGATGGTGCTGCGCTGGAGTGACGGC 1120

1041 GCAGACTTAAACTGGACTCGCGTAAAAATGCGCGGCCTCTTTTGGCGGAGCGCCACTACCACGACGCGACCTCACTGCCG 1120

DdeI MspI

1121 AGTTATCTGGAAGATCAGGATATGTGGCGGATGAGCGGCATTTTCCGTGACGTCTCGTTGCTGCATAAACCGACTACACA 1200

1121 TCAATAGACCTTCTAGTCCTATACACCGCCTACTCGCCGTAAAAGGCACTGCAGAGCAACGACGTATTTGGCTGATGTGT 1200

Sau3AI AatII

DpnI

1201 AATCAGCGATTTCCATGTTGCCACTCGCTTTAATGATGATTTCAGCCGCGCTGTACTGGAGGCTGAAGTTCAGATGTGCG 1280

1201 TTAGTCGCTAAAGGTACAACGGTGAGCGAAATTACTACTAAAGTCGGCGCGACATGACCTCCGACTTCAAGTCTACACGC 1280

RsaI

1281 GCGAGTTGCGTGACTACCTACGGGTAACAGTTTCTTTATGGCAGGGTGAAACGCAGGTCGCCAGCGGCACCGCGCCTTTC 1360

1281 CGCTCAACGCACTGATGGATGCCCATTGTCAAAGAAATACCGTCCCACTTTGCGTCCAGCGGTCGCCGTGGCGCGGAAAG 1360

1361 GGCGGTGAAATTATCGATGAGCGTGGTGGTTATGCCGATCGCGTCACACTACGTCTGAACGTCGAAAACCCGAAACTGTG 1440

1361 CCGCCACTTTAATAGCTACTCGCACCACCAATACGGCTAGCGCAGTGTGATGCAGACTTGCAGCTTTTGGGCTTTGACAC 1440

TaqI Sau3AI TaqI

DpnI

PvuI

1441 GAGCGCCGAAATCCCGAATCTCTATCGTGCGGTGGTTGAACTGCACACCGCCGACGGCACGCTGATTGAAGCAGAAGCCT 1520

1441 CTCGCGGCTTTAGGGCTTAGAGATAGCACGCCACCAACTTGACGTGTGGCGGCTGCCGTGCGACTAACTTCGTCTTCGGA 1520

HinfI

1521 GCGATGTCGGTTTCCGCGAGGTGCGGATTGAAAATGGTCTGCTGCTGCTGAACGGCAAGCCGTTGCTGATTCGAGGCGTT 1600

1521 CGCTACAGCCAAAGGCGCTCCACGCCTAACTTTTACCAGACGACGACGACTTGCCGTTCGGCAACGACTAAGCTCCGCAA 1600

HinfI

TaqI

1601 AACCGTCACGAGCATCATCCTCTGCATGGTCAGGTCATGGATGAGCAGACGATGGTGCAGGATATCCTGCTGATGAAGCA 1680

1601 TTGGCAGTGCTCGTAGTAGGAGACGTACCAGTCCAGTACCTACTCGTCTGCTACCACGTCCTATAGGACGACTACTTCGT 1680

HincII EcoRV

HpaI

1681 GAACAACTTTAACGCCGTGCGCTGTTCGCATTATCCGAACCATCCGCTGTGGTACACGCTGTGCGACCGCTACGGCCTGT 1760

1681 CTTGTTGAAATTGCGGCACGCGACAAGCGTAATAGGCTTGGTAGGCGACACCATGTGCGACACGCTGGCGATGCCGGACA 1760

RsaI

1761 ATGTGGTGGATGAAGCCAATATTGAAACCCACGGCATGGTGCCAATGAATCGTCTGACCGATGATCCGCGCTGGCTACCG 1840

1761 TACACCACCTACTTCGGTTATAACTTTGGGTGCCGTACCACGGTTACTTAGCAGACTGGCTACTAGGCGCGACCGATGGC 1840

SspI HinfI Sau3AI MspI

DpnI

1841 GCGATGAGCGAACGCGTAACGCGAATGGTGCAGCGCGATCGTAATCACCCGAGTGTGATCATCTGGTCGCTGGGGAATGA 1920

1841 CGCTACTCGCTTGCGCATTGCGCTTACCACGTCGCGCTAGCATTAGTGGGCTCACACTAGTAGACCAGCGACCCCTTACT 1920

MluI Sau3AI BclI HinfI

DpnI Sau3AI

PvuI DpnI

1921 ATCAGGCCACGGCGCTAATCACGACGCGCTGTATCGCTGGATCAAATCTGTCGATCCTTCCCGCCCGGTGCAGTATGAAG 2000

1921 TAGTCCGGTGCCGCGATTAGTGCTGCGCGACATAGCGACCTAGTTTAGACAGCTAGGAAGGGCGGGCCACGTCATACTTC 2000

Sau3AI TaqI NciI

DpnI Sau3AI MspI

DpnI

2001 GCGGCGGAGCCGACACCACGGCCACCGATATTATTTGCCCGATGTACGCGCGCGTGGATGAAGACCAGCCCTTCCCGGCT 2080

2001 CGCCGCCTCGGCTGTGGTGCCGGTGGCTATAATAAACGGGCTACATGCGCGCGCACCTACTTCTGGTCGGGAAGGGCCGA 2080

RsaI NciI

BssHII MspI

2081 GTGCCGAAATGGTCCATCAAAAAATGGCTTTCGCTACCTGGAGAGACGCGCCCGCTGATCCTTTGCGAATACGCCCACGC 2160

2081 CACGGCTTTACCAGGTAGTTTTTTACCGAAAGCGATGGACCTCTCTGCGCGGGCGACTAGGAAACGCTTATGCGGGTGCG 2160

EcoRII Sau3AI

DpnI

2161 GATGGGTAACAGTCTTGGCGGTTTCGCTAAATACTGGCAGGCGTTTCGTCAGTATCCCCGTTTACAGGGCGGCTTCGTCT 2240

2161 CTACCCATTGTCAGAACCGCCAAAGCGATTTATGACCGTCCGCAAAGCAGTCATAGGGGCAAATGTCCCGCCGAAGCAGA 2240

2241 GGGACTGGGTGGATCAGTCGCTGATTAAATATGATGAAAACGGCAACCCGTGGTCGGCTTACGGCGGTGATTTTGGCGAT 2320

2241 CCCTGACCCACCTAGTCAGCGACTAATTTATACTACTTTTGCCGTTGGGCACCAGCCGAATGCCGCCACTAAAACCGCTA 2320

Sau3AI

DpnI

2321 ACGCCGAACGATCGCCAGTTCTGTATGAACGGTCTGGTCTTTGCCGACCGCACGCCGCATCCAGCGCTGACGGAAGCAAA 2400

2321 TGCGGCTTGCTAGCGGTCAAGACATACTTGCCAGACCAGAAACGGCTGGCGTGCGGCGTAGGTCGCGACTGCCTTCGTTT 2400

Sau3AI Eco47III

DpnI

PvuI

2401 ACACCAGCAGCAGTTTTTCCAGTTCCGTTTATCCGGGCAAACCATCGAAGTGACCAGCGAATACCTGTTCCGTCATAGCG 2480

2401 TGTGGTCGTCGTCAAAAAGGTCAAGGCAAATAGGCCCGTTTGGTAGCTTCACTGGTCGCTTATGGACAAGGCAGTATCGC 2480

MspI TaqI

NciI

2481 ATAACGAGCTCCTGCACTGGATGGTGGCGCTGGATGGTAAGCCGCTGGCAAGCGGTGAAGTGCCTCTGGATGTCGCTCCA 2560

2481 TATTGCTCGAGGACGTGACCTACCACCGCGACCTACCATTCGGCGACCGTTCGCCACTTCACGGAGACCTACAGCGAGGT 2560

AluI

SacI

2561 CAAGGTAAACAGTTGATTGAACTGCCTGAACTACCGCAGCCGGAGAGCGCCGGGCAACTCTGGCTCACAGTACGCGTAGT 2640

2561 GTTCCATTTGTCAACTAACTTGACGGACTTGATGGCGTCGGCCTCTCGCGGCCCGTTGAGACCGAGTGTCATGCGCATCA 2640

MspI MspI RsaI

NciI MluI

2641 GCAACCGAACGCGACCGCATGGTCAGAAGCCGGGCACATCAGCGCCTGGCAGCAGTGGCGTCTGGCGGAAAACCTCAGTG 2720

2641 CGTTGGCTTGCGCTGGCGTACCAGTCTTCGGCCCGTGTAGTCGCGGACCGTCGTCACCGCAGACCGCCTTTTGGAGTCAC 2720

MspI EcoRII DdeI

NciI

2721 TGACGCTCCCCGCCGCGTCCCACGCCATCCCGCATCTGACCACCAGCGAAATGGATTTTTGCATCGAGCTGGGTAATAAG 2800

2721 ACTGCGAGGGGCGGCGCAGGGTGCGGTAGGGCGTAGACTGGTGGTCGCTTTACCTAAAAACGTAGCTCGACCCATTATTC 2800

TaqI

AluI

2801 CGTTGGCAATTTAACCGCCAGTCAGGCTTTCTTTCACAGATGTGGATTGGCGATAAAAAACAACTGCTGACGCCGCTGCG 2880

2801 GCAACCGTTAAATTGGCGGTCAGTCCGAAAGAAAGTGTCTACACCTAACCGCTATTTTTTGTTGACGACTGCGGCGACGC 2880

BglI

2881 CGATCAGTTCACCCGTGCACCGCTGGATAACGACATTGGCGTAAGTGAAGCGACCCGCATTGACCCTAACGCCTGGGTCG 2960

2881 GCTAGTCAAGTGGGCACGTGGCGACCTATTGCTGTAACCGCATTCACTTCGCTGGGCGTAACTGGGATTGCGGACCCAGC 2960

Sau3AI ApaLI EcoRII

DpnI TaqI

2961 AACGCTGGAAGGCGGCGGGCCATTACCAGGCCGAAGCAGCGTTGTTGCAGTGCACGGCAGATACACTTGCTGATGCGGTG 3040

2961 TTGCGACCTTCCGCCGCCCGGTAATGGTCCGGCTTCGTCGCAACAACGTCACGTGCCGTCTATGTGAACGACTACGCCAC 3040

EcoRII ApaLI

3041 CTGATTACGACCGCTCACGCGTGGCAGCATCAGGGGAAAACCTTATTTATCAGCCGGAAAACCTACCGGATTGATGGTAG 3120

3041 GACTAATGCTGGCGAGTGCGCACCGTCGTAGTCCCCTTTTGGAATAAATAGTCGGCCTTTTGGATGGCCTAACTACCATC 3120

MluI MspI MspI

3121 TGGTCAAATGGCGATTACCGTTGATGTTGAAGTGGCGAGCGATACACCGCATCCGGCGCGGATTGGCCTGAACTGCCAGC 3200

3121 ACCAGTTTACCGCTAATGGCAACTACAACTTCACCGCTCGCTATGTGGCGTAGGCCGCGCCTAACCGGACTTGACGGTCG 3200

MspI PvuII

AluI

3201 TGGCGCAGGTAGCAGAGCGGGTAAACTGGCTCGGATTAGGGCCGCAAGAAAACTATCCCGACCGCCTTACTGCCGCCTGT 3280

3201 ACCGCGTCCATCGTCTCGCCCATTTGACCGAGCCTAATCCCGGCGTTCTTTTGATAGGGCTGGCGGAATGACGGCGGACA 3280

3281 TTTGACCGCTGGGATCTGCCATTGTCAGACATGTATACCCCGTACGTCTTCCCGAGCGAAAACGGTCTGCGCTGCGGGAC 3360

3281 AAACTGGCGACCCTAGACGGTAACAGTCTGTACATATGGGGCATGCAGAAGGGCTCGCTTTTGCCAGACGCGACGCCCTG 3360

Sau3AI RsaI

DpnI

3361 GCGCGAATCGAATTATGGCCCACACCAGTGGCGCGGCGACTTCCAGTTCAACATCAGCCGCTACAGTCAACAGCAACTGA 3440

3361 CGCGCTTAGCTTAATACCGGGTGTGGTCACCGCGCCGCTGAAGGTCAAGTTGTAGTCGGCGATGTCAGTTGTCGTTGACT 3440

HinfI HincII

TaqI

3441 TGGAAACCAGCCATCGCCATCTGCTGCACGCGGAAGAAGGCACATGGCTGAATATCGACGGTTTCCATATGGGGATTGGT 3520

3441 ACCTTTGGTCGGTAGCGGTAGACGACGTGCGCCTTCTTCCGTGTACCGACTTATAGCTGCCAAAGGTATACCCCTAACCA 3520

TaqI NdeI

3521 GGCGACGACTCCTGGAGCCCGTCAGTATCGGCGGAATTCCAGCTGAGCGCCGGTCGCTACCATTACCAGTTGGTCTGGTG 3600

3521 CCGCTGCTGAGGACCTCGGGCAGTCATAGCCGCCTTAAGGTCGACTCGCGGCCAGCGATGGTAATGGTCAACCAGACCAC 3600

HinfI EcoRI PvuII MspI

EcoRII AluI

DdeI

PGK-1 promoter

3601 TCAAAAATAATAATAACCGGGCAGGGGGGATCCCCCAGATCATCGAATTCTACCGGGTAGGGGAGGCGCTTTTCCCAAGG 3680

3601 AGTTTTTATTATTATTGGCCCGTCCCCCCTAGGGGGTCTAGTAGCTTAAGATGGCCCATCCCCTCCGCGAAAAGGGTTCC 3680

MspI BamHI Sau3AI EcoRI MspI

NciI Sau3AI DpnI NciI

DpnI TaqI

3681 CAGTCTGGAGCATGCGCTTTAGCAGCCCCGCTGGGCACTTGGCGCTACACAAGTGGCCTCTGGCCTCGCACACATTCCAC 3760

3681 GTCAGACCTCGTACGCGAAATCGTCGGGGCGACCCGTGAACCGCGATGTGTTCACCGGAGACCGGAGCGTGTGTAAGGTG 3760

SphI

3761 ATCCACCGGTAGGCGCCAACCGGCTCCGTTCTTTGGTGGCCCCTTCGCGCCACCTTCTACTCCTCCCCTAGTCAGGAAGT 3840

3761 TAGGTGGCCATCCGCGGTTGGCCGAGGCAAGAAACCACCGGGGAAGCGCGGTGGAAGATGAGGAGGGGATCAGTCCTTCA 3840

MspI NarI MspI

3841 TCCCCCCCGCCCCGCAGCTCGCGTCGTGCAGGACGTGACAAATGGAAGTAGCACGTCTCACTAGTCTCGTGCAGATGGAC 3920

3841 AGGGGGGGCGGGGCGTCGAGCGCAGCACGTCCTGCACTGTTTACCTTCATCGTGCAGAGTGATCAGAGCACGTCTACCTG 3920

AluI SpeI

3921 AGCACCGCTGAGCAATGGAAGCGGGTAGGCCTTTGGGGCAGCGGCCAATAGCAGCTTTGCTCCTTCGCTTTCTGGGCTCA 4000

3921 TCGTGGCGACTCGTTACCTTCGCCCATCCGGAAACCCCGTCGCCGGTTATCGTCGAAACGAGGAAGCGAAAGACCCGAGT 4000

DdeI StuI BglI AluI DdeI

4001 GAGGCTGGGAAGGGGTGGGTCCGGGGGCGGGCTCAGGGGCGGGCTCAGGGGCGGGGCGGGCGCCCGAAGGTCCTCCGGAG 4080

4001 CTCCGACCCTTCCCCACCCAGGCCCCCGCCCGAGTCCCCGCCCGAGTCCCCGCCCCGCCCGCGGGCTTCCAGGAGGCCTC 4080

MspI DdeI DdeI NarI EcoO109I

NciI MspI

Neo*

4081 GCCCGGCATTCTGCACGCTTCAAAAGCGCACGTCTGCCGCGCTGTTCTCCTCTTCCTCATCTCCGGGCCTTTCGACCTGC 4160

4081 CGGGCCGTAAGACGTGCGAAGTTTTCGCGTGCAGACGGCGCGACAAGAGGAGAAGGAGTAGAGGCCCGGAAAGCTGGACG 4160

NciI MspI TaqI

MspI NciI

4161 AGCCAATATGGGATCGGCCATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCT 4240

4161 TCGGTTATACCCTAGCCGGTAACTTGTTCTACCTAACGTGCGTCCAAGAGGCCGGCGAACCCACCTCTCCGATAAGCCGA 4240

PstI Sau3AI MspI

DpnI

4241 ATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTT 4320

4241 TACTGACCCGTGTTGTCTGTTAGCCGACGAGACTACGGCGGCACAAGGCCGACAGTCGCGTCCCCGCGGGCCAAGAAAAA 4320

MspI NarI

NciI

MspI

4321 GTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGT 4400

4321 CAGTTCTGGCTGGACAGGCCACGGGACTTACTTGACGTCCTGCTCCGTCGCGCCGATAGCACCGACCGGTGCTGCCCGCA 4400

MspI PstI

4401 TCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATC 4480

4401 AGGAACGCGTCGACACGAGCTGCAACAGTGACTTCGCCCTTCCCTGACCGACGATAACCCGCTTCACGGCCCCGTCCTAG 4480

PvuII TaqI MspI Sau3AI

AluI NciI DpnI

4481 TCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCG 4560

4481 AGGACAGTAGAGTGGAACGAGGACGGCTCTTTCATAGGTAGTACCGACTACGTTACGCCGCCGACGTATGCGAACTAGGC 4560

Sau3AI

DpnI

MspI

4561 GCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCA 4640

4561 CGATGGACGGGTAAGCTGGTGGTTCGCTTTGTAGCGTAGCTCGCTCGTGCATGAGCCTACCTTCGGCCAGAACAGCTAGT 4640

TaqI TaqI RsaI MspI TaqI

Sau3AI

DpnI

4641 GGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCG 4720

4641 CCTACTAGACCTGCTTCTCGTAGTCCCCGAGCGCGGTCGGCTTGACAAGCGGTCCGAGTTCCGCGCGTACGGGCTGCCGC 4720

Sau3AI EcoRII BssHII SphI

DpnI

4721 ATGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGAC 4800

4721 TACTAGAGCAGCACTGGGTACCGCTACGGACGAACGGCTTATAGTACCACCTTTTACCGGCGAAAAGACCTAAGTAGCTG 4800

Sau3AI NcoI HinfI

DpnI TaqI

4801 TGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGA 4880

4801 ACACCGGCCGACCCACACCGCCTGGCGATAGTCCTGTATCGCAACCGATGGGCACTATAACGACTTCTCGAACCGCCGCT 4880

MspI AluI

4881 ATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACG 4960

4881 TACCCGACTGGCGAAGGAGCACGAAATGCCATAGCGGCGAGGGCTAAGCGTCGCGTAGCGGAAGATAGCGGAAGAACTGC 4960

HinfI

Mouse Hprt SD splice site

4961 AGTTCTTCTGAGGGGATCAATTCTCTAGCGCGCCTGAGTACTTCAGGGATTTGAATGTAAGTAATGCTTTTTTTTTTCTC 5040

4961 TCAAGAAGACTCCCCTAGTTAAGAGATCGCGCGGACTCATGAAGTCCCTAAACTTACATTCATTACGAAAAAAAAAAGAG 5040

DdeI Sau3AI BssHII ScaI

DpnI DdeI

RsaI

5041 GCTCTCGTTTTTCAAAAACCCACATAAAAATTGAGGAAAGGGAAGAATTGTTTTCTCCTTCCAGCACCTCGTAATTTGAC 5120

5041 CGAGAGCAAAAAGTTTTTGGGTGTATTTTTAACTCCTTTCCCTTCTTAACAAAAGAGGAAGGTCGTGGAGCATTAAACTG 5120

5121 CCGACTGATGGTTCCCATTAGTCACATAAAGCTGTAGTCAAGTACAGACGTCCTTAGAACTGGAACTTGGCCAGGCTACG 5200

5121 GGCTGACTACCAAGGGTAATCAGTGTATTTCGACATCAGTTCATGTCTGCAGGAATCTTGACCTTGAACCGGTCCGATGC 5200

AluI RsaI AatII EcoRII

DdeI

5201 GTGACACTTCTTGCTGGCTGAAATAGTTGAACAGCTTTAATATAGGCGCGCTAGAGTCGACCTCGAGAAGGAGTGAGGGC 5280

5201 CACTGTGAAGAACGACCGACTTTATCAACTTGTCGAAATTATATCCGCGCGATCTCAGCTGGAGCTCTTCCTCACTCCCG 5280

AluI BssHII HinfI XhoI

SalI TaqI

TaqI

HincII

5281 TGGATAAAGGGAGGATCGAGGCGGGGTCGAACGAGGAGGTTCAAGGGGGAGAGACGGGGCGGATGGAGGAAGAGGAGGCG 5360

5281 ACCTATTTCCCTCCTAGCTCCGCCCCAGCTTGCTCCTCCAAGTTCCCCCTCTCTGCCCCGCCTACCTCCTTCTCCTCCGC 5360

Sau3AI TaqI

DpnI

TaqI

5361 GAGGCTTAGGGTGTACAAAGGGCTTGACCCAGGGAGGGGGGTCAAAAGCCAAGGCTTCCCAGGTCACGATGTAGGGGACC 5440

5361 CTCCGAATCCCACATGTTTCCCGAACTGGGTCCCTCCCCCCAGTTTTCGGTTCCGAAGGGTCCAGTGCTACATCCCCTGG 5440

DdeI RsaI EcoRII EcoRII EcoO109I

EcoRII

5441 TGGTCTGGGTGTCCATGCGGGCCAGGTGAAAAGACCTTGATCTTAACCTGGGTGATGAGGTCTCGGTTAAAGGTGCCGTC 5520

5441 ACCAGACCCACAGGTACGCCCGGTCCACTTTTCTGGAACTAGAATTGGACCCACTACTCCAGAGCCAATTTCCACGGCAG 5520

EcoRII Sau3AI EcoRII

DpnI

5521 TCGCGGCCATCCGACGTTAAAGGTTGGCCATTCTGCAGAGCAGAAGGTAACCCAACGTCTCTTCTTGACATCTACCGACT 5600

5521 AGCGCCGGTAGGCTGCAATTTCCAACCGGTAAGACGTCTCGTCTTCCATTGGGTTGCAGAGAAGAACTGTAGATGGCTGA 5600

PstI

5601 GGTTGTGAGCGATCCGCTCGACATCTTTCCAGTGACCTAAGGTCAAACTTAAGGGAGTGGTAACAGTCTGGCCCATATTC 5680

5601 CCAACACTCGCTAGGCGAGCTGTAGAAAGGTCACTGGATTCCAGTTTGAATTCCCTCACCATTGTCAGACCGGGTATAAG 5680

Sau3AI TaqI DdeI

DpnI

5681 TCAGACAAATACAGAAACACAGTCAGACAGAGACAACACAGAACGATGCTGCAGCAGACAAGACGCGCGGCGCGGCTTCG 5760

5681 AGTCTGTTTATGTCTTTGTGTCAGTCTGTCTCTGTTGTGTCTTGCTACGACGTCGTCTGTTCTGCGCGCCGCGCCGAAGC 5760

DdeI PstI

5761 GTCCCAAACCGAAAGCAAAAATTCAGACGGAGGCGGGAACTGTTTTAGGTTCTCGTCTCCTACCAGAACCACATATCCCT 5840

5761 CAGGGTTTGGCTTTCGTTTTTAAGTCTGCCTCCGCCCTTGACAAAATCCAAGAGCAGAGGATGGTCTTGGTGTATAGGGA 5840

5841 CCTCTAAGGGGGGTGCACCAAAGAGTCCAAAACGATCGGGATTTTTGGACTCAGGTCGGGCCACAAAAACGGCCCCCGAA 5920

5841 GGAGATTCCCCCCACGTGGTTTCTCAGGTTTTGCTAGCCCTAAAAACCTGAGTCCAGCCCGGTGTTTTTGCCGGGGGCTT 5920

DdeI ApaLI HinfI Sau3AI HinfI

DpnI DdeI

PvuI

5921 GTCCCTGGGACGTCTCCCAGGGTTGCGGCCGGGTGTTCCGAACTCGTCAGTTCCACCACGGGTCCGCCAGATACAGAGCT 6000

5921 CAGGGACCCTGCAGAGGGTCCCAACGCCGGCCCACAAGGCTTGAGCAGTCAAGGTGGTGCCCAGGCGGTCTATGTCTCGA 6000

EcoRII AatII MspI AluI

EcoRII NciI

6001 AGTTAGCTAACTAGTACCGACGCAGGCGCATAAAATCAGTCATAGACACTAGACAATCGGACAGACACAGATAAGTTGCT 6080

6001 TCAATCGATTGATCATGGCTGCGTCCGCGTATTTTAGTCAGTATCTGTGATCTGTTAGCCTGTCTGTGTCTATTCAACGA 6080

AluI RsaI

SpeI

5’LTR

6081 GGCCAGCTTACCTCCCGGTGGTGGGTCGGTGGTCCCTGGGCAGGGGTCTCCCGATCCCGGACGAGCCCCCAAATGAAAGA 6160

6081 CCGGTCGAATGGAGGGCCACCACCCAGCCACCAGGGACCCGTCCCCAGAGGGCTAGGGCCTGCTCGGGGGTTTACTTTCT 6160

AluI NciI EcoRII Sau3AI

MspI DpnI

NciI

MspI

6161 CCCCCGCTGACGGGTAGTCAATCACTCAGAGGAGACCCTCCCAAGGAACAGCGAGACCACAAGTCGGATGCAACTGCAAG 6240

6161 GGGGGCGACTGCCCATCAGTTAGTGAGTCTCCTCTGGGAGGGTTCCTTGTCGCTCTGGTGTTCAGCCTACGTTGACGTTC 6240

DdeI

6241 AGGGTTTATTGGATACACGGGTACCCGGGCGACTCAGTCAATCGGAGGACTGGCGCCCCGAGTGAGGGGTTGTGGGCTCT 6320

6241 TCCCAAATAACCTATGTGCCCATGGGCCCGCTGAGTCAGTTAGCCTCCTGACCGCGGGGCTCACTCCCCAACACCCGAGA 6320

RsaI HinfI NarI

KpnI DdeI

NciI

MspI

SmaI

NciI

6321 TTTATTGAGCTTAAGCTAGCTTGCCAAACCTACAGGTGGGGTCTTTCA 6368

6321 AAATAACTCGAATTCGATCGAACGGTTTGGATGTCCACCCCAGAAAGT 6368

AluI AluI

AluI

NheI

Enzymes that do not cut:

______

ApaI, DraI, MunI, NotI, NruI, NsiI, SacII, SnaBI, SrfI, VspI, XbaI, XmnI