Table S2. Technical analysis of ESTs in F. graminearum and F. culmorum found in MIPS FGDB.
GenBank
accession
FG ORF DNA1 Sequence coordinates2 number Comments3
No ORF hit (FG) 1.16 – (92005 – 92182) DY763143 (Fs14662935), 3E-16
FG00551 (FG) 1.22 – (86678 – 86908) DY763076
FG00775-ext (FG) 1.35 – (223361 – 223535) DY763077
FG12075 (FC) 1.35 – (260938 – 261323) DY763078
FG01004-ext (FC) 1.48 – (21581 – 21885) DY588253
FG01047(FG) 1.52 – (30977 – 31195) DY763079 In conflict with MIPS
intron annotation
FG01092-ext (FG) 1.53 – (47975 – 48206) DY763133 A possible extension. PA
FG01101 (FC) 1.54 – (6400 – 6649) ES316460
No ORF hit (FG) 1.60 – (24498 – 24582) DY763134 Gibberella zeae cDNA clone (CV827945). 1E-09
FG01226-ext (FC) 1.61 – (18996 – 19306) DY763080
No ORF hit (FC) 1.62 – (47958 – 48093) DY763131 14-3-3 family protein,
(GzCon[0751]), 7E-70
FG01351 (FC) 1.66 – (53251 – 53472) DY763081
FG01417 (FC) 1.71 – (12091 – 12332) / DY588265 Intron from 12333 - 12381
1.71 – (12382 – 12517) Confirming MIPS intron
annotation. PA
FG01584-ext (FG) 1.79 – (95806 – 96152) DY588287 PA
FG04336 (FG) 1.87 – (12828 – 12885) DY763082
No ORF hit (FG) 1.87 – (71585 – 71786) DY588295 (Gz47835827), E-115
FG01749 (FG) 1.92 – (36388 – 36725) DY588296
FG01826 (FG) 1.94 – (4344 – 4497) / DY588297 Intron from 4498 - 4544
1.94 – (4545 – 4637) Confirming MIPS intron
annotation
FG01837-ext (FC) 1.94 – (29558 – 29645) DY763122 PA
No ORF hit (FC) 1.103 – (102642 – 102722) DY763123 Unknown
FG01959 (FG) 1.104 – (101858 – 102037) DY763084
FG02022-ext (FG) 1.110 – (20581 – 20792) DY763085
FG02119 (FG) 1.111 – (117609 – 117667) DY763086
FG02247-ext (FG) 1.114 – (69924 – 70222) DY763087 PA
FG02409-ext (FG) 1.122 – (12807 – 12985) DY763088
FG02582 (FG) 1.128 – (71175 – 71523) DY763089 In conflict with MIPS
intron annotation. PA
FG02666 (FG) 1.132 – (171547 – 171791) DY588298
FG02667-ext (FC) 1.132 – (172760 – 172954) DY588299
FG02668 (FC) 1.132 – (178017 – 178280) DY588254
FG02692-ext (FC) 1.133 – (30645 – 30741) DY763090
FG02705(FG) 1.135 – (13506 – 13702) DY763091
FG02795-ext (FC) 1.141 – (9215 – 9518) DY763092 PA
FGD142-220-ext(FG) 1.142 – (50491 – 50675) DY763093 PA
FG02894 (FC) 1.142 – (148358 – 148736) DY588255
FG03081 (FC) 1.147 – (96409 – 96771) DY588256
FG03086 (FG) 1.147 – (108665 – 108706) DY588258
FG03184 (FG) 1.148 – (246279 – 246541) DY588258
FG03190-ext (FG) 1.148 – (263999 – 264260) / DY588259 Intron from 264261 -
1.148 – (264312 – 264482) 264311. In conflict with
MIPS intron annotation
FG03199-ext (FG) 1.149 – (17323 – 17443) DY588260 PA
FG03650(FC) 1.160 – (230723 – 231113) DY763094
FG03735 (FG) 1.162 – (71423 – 71543) DY588261 In conflict with MIPS
intron annotation. PA
FG03738-ext (FG) 1.162 – (82539 – 82941) DY588262 PA
FG03831 (FG) 1.166 – (39278 – 39485) DY763095
No ORF hit (FG) 1.167 – (33252 – 33549) DY763142 Related to hypothetical
protein FG04501
(XP_384677), 4E-24. PA
FG03995(FG) 1.168 – (322348 – 322582) DY763096
FG04230-ext (FG) 1.185 – (148526 – 148743) DY763097 In conflict with MIPS
intron annotation.
FG04405-ext (FC) 1.190 – (85129 – 85362) DY763098 PA
No ORF hit (FG) 1.192 – (15825 – 15989) DY588276 Unknown
FG04512 (FG) 1.192 – (206995 – 207241) DY763099
FG04924-ext (FC) 1.199 – (89707 – 89789) DY763100 In conflict with MIPS
intron annotation. PA
FG05061-ext (FC) 1.203 – (59778 – 60280) DY588264 Alternative splicing
1.203 – (59778 – 60025) / DY588263 Intron from 60026 - 60074
1.203 – (60075 – 60280)
FG05095 (FG) 1.205 – (19354 – 19618) DY588266
No ORF hit (FG) 1.212 – (127510 – 127859) DY763138 Unknown
FG05290 (FG) 1.212 – (229180 – 229349) DY763101
No ORF hit (FC) 1.213 – (18683 – 18975) DY588267 Mitochondrial outer
membrane protein involved in membrane fission (GzCon[0585]), 9E-83
FG05422 (FC) 1.224 – (2300 – 2488) DY763102
No ORF hit (FC) 1.227 – (28138 – 28381) DY763124 Unknown. PA
No ORF hit (FG) 1.229 – (24151 – 24346) DY763136 (GzCon[2149]), 3E-90.PA
FG05636 (FC) 1.231 – (72451 – 72684) DY763103
No ORF hit (FC) 1.231 – (120756 – 120835) DY763125 Unknown
FG05821-ext (FG) 1.233 – (91186 – 91418) DY588269 PA
FG05890-ext (FC) 1.233 – (298463 – 298678) DY763104
FG05928-ext (FG) 1.237 – (87542 – 87860) / DY588270 Intron from 87861-87922.
1.237 – (87923 – 88035) Confirming MIPS intron
annotation
FG05929-ext (FG) 1.237 – (89064 – 89165) DY588271 A possible extension. PA
FG06070 (FC) 1.241 – (42142 – 42174) / DY588272 Intron from 42175-42238.
1.241 – (42239 – 42275) In conflict with MIPS
intron annotation
No ORF hit (FC) 1.246 – (75513 – 75883) DY763130 Unknown. PA
No ORF hit (FG) 1.247 – (55637 – 55816) DY763135 Unknown. PA
FG06231 (FC) 1.248 – (9943 – 10118) / DY763105 Intron from 10119-10169.
1.248 – (10170 – 10189) Confirming MIPS intron
annotation.
FG06416-ext (FG) 1.258 – (101655 – 101712) EB077975 A possible extension
FG06583-ext (FG) 1.259 – (452114 – 452292) DY763146 A possible extension. PA
No ORF hit (FG) 1.266 – (156750 – 156928) DY588273 Unknown. PA
No ORF hit (FC) 1.270 – (15885 – 15954) DY763128 Gibberella zeae cDNA (CD456267). 6E-129.
FG06931 (FG) 1.285 – (43456 – 43510) / DY763107 Intron from 43511-43566.
1.285 – (43567 – 43641) Confirming MIPS intron
annotation
FG07075-ext (FG) 1.297 – (2138 – 2273) / DY763108 Genome reorganization,
1.297 – (15849 – 15936) very long (~ 13476 bp)
gap. PA
FG07076-ext (FG) 1.297 – (20066 – 20180) DY588275 PA
FG07104 (FG) 1.298 – (82046 – 82401) DY588277
FG07177-ext (FC) 1.302 – (8994 – 9198) / DY763083 Introns from 9199 - 9204
1.302 – (9205 – 9221) / and 9222 - 9227. In 1.302 – (9228 – 9353) conflict with MIPS intron
annotation
FG07442 (FG) 1.310 – (66965 – 67302) DY588278 In conflict with MIPS
intron annotation
No ORF hit (FC) 1.310 – (195402 – 195635) DY588279 Unknown
FG07551 (FG) 1.314 – (43061 – 43320) DY763109 In conflict with MIPS
intron annotation
FG07821(FG) 1.320 – (145377 – 145438) DY763140 In conflict with MIPS
intron annotation. PA
FGD321-640 (FC) 1.321 – (169058 – 169423) DY763121 In conflict with MIPS
intron annotation
FG08245-ext (FG) 1.329 – (199666 – 199898) DY588280 PA
Fg4A76 (FC) 1.335 – (88255 – 88499) DY588281 PA
FG08540(FC) 1.345 – (10215 – 10510) DY763110
FG08597-ext (FG) 1.348 – (59658 – 59896) DY763111 PA
No ORF hit (FG) 1.349 – (78544 – 78640) DY763140 (Gz47836378), 2E-49. PA
FG08710-ext (FC) 1.353 – (20581 – 20921) DY588282 A possible extension
FG08811-ext (FC) 1.355 – (133410 – 133589) DY588283 EF1A
FG08844(FC) 1.358 – (7238 – 7493) DY763106
FG08847-ext (FC) 1.358 – (19738 – 19804) / DY763112 Introns from 19805-19853
1.358 – (19854 – 19913) / and 19914 - 19964. Both
1.358 – (19965 – 20150) confirming and in conflict
with MIPS intron
annotation
No ORF hit (FG) 1.361 – (13097 – 13267) DY588284 Gibberella moniliformis cDNA (DR616112).5E-33
FG09103 (FG) 1.370 – (170788 – 170921) / DY588286 Intron from 170922 - 1.370 – (170974 – 171025) 170973. Confirming MIPS
intron annotation
FG09355-ext (FG) 1.383 – (26422 – 26465) DY588288
No ORF hit (FC) 1.402 – (21363 – 21594) DY763126 Unknown
FG09840-ext (FG) 1.406 – (218821 – 219145) DY588289 In conflict with MIPS
intron annotation. PA
FG09866(FG) 1.409 – (23623 – 23749) / DY763113 Intron from 23750-23802.
1.409 – (23803 – 23877) Confirming MIPS intron
annotation
FG09913 (FG) 1.411 – (108392 – 108833) DY588290 In conflict with MIPS
intron annotation
FG09976 (FC) 1.415 – (17825 – 18151) DY588291
FG10084 (FG) 1.417 – (211586 – 211792) DY588292
FGD417-780 (FG) 1.417 – (219399 – 219586) DY588293 In conflict with MIPS
intron annotation
FG10292(FC) 1.425 – (114347 – 114408) DY763114
FG10313-ext (FC) 1.425 – (159147 – 159490) DY763115 MGV1. PA
No ORF hit (FG) 1.430 – (8124 – 8301) DY763145 Unknown
FG10387-ext (FG) 1.433 – (73302 – 73612) DY588274 PA
FG10423-ext (FC) 1.434 – (21062 – 21284) DY763116 PA
FG10589-ext (FG) 1.444 – (18983 – 19215) DY588294
FGD447-70 (FC) 1.447 – (22370 – 22720) DY763117
FG10758-ext (FC) 1.447 – (133745 – 133953) DY763127
FG10997-ext (FG) 1.458 – (97723 – 97788) DY763118 PA
FG11012-ext (FG) 1.458 – (141228 – 141409) / DY763119 Intron from 141400 -
1.458 – (141461 – 141493) 141460. Confirming MIPS
intron annotation. PA
No ORF hit (FG) 1.458 – (207151 – 207330) DY763137 Unknown
No ORF hit (FC) 1.458 – (72543 – 72634) DY763129 Unknown
FG11190-ext (FG) 1.460 – (103341 – 103444) / DY763120 Intron from 103445 -
1.460 – (103495 – 103652) 103494. Confirming MIPS
intron annotation
FG11310-ext (FG) 1.464 – (119003 – 119209) DY588268
1The ESTs are marked with the gene name according to the annotated MIPS FGDB if they are situated in the coordinates of the gene sequence, or with an additional -ext, e.g. FG00775-ext, if they are putative extensions of the predicted genes. The “No ORF hits” are completely outside the MIPS gene annotations. FG = F. graminearum, FC = F. culmorum. 2All sequence coordinates are given on the plus strain. 3The “No ORF hits” are described with Blast results (Cogeme, NCBI), with Cogeme Unisequence ID or GenBank accession number for the best match in parentheses. E values greater than E-05 were listed as “Unknown”. The commented polyadenylation tails (PA) begin at the end of the GenBank sequence.
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