AppendixS1

Sample localities and the number of examined materials of Cardamine hirsutathat cover present distribution of the species in Japan.Site ID number, sampling site, latitude, longitude, numbers of plants for genotyping of SSR markers, numbers of flowers and plants for counting of stamen numbers, mean of observed heterozygosity (HO), andmean of unbiased expected heterozygosity (HE) are listed.

Site
ID / Sampling site
(Locality, Prefecture) / Latitude
(N) / Longitude
(E) / No. of individuals for genotyping / No. of flowers (plants) for examination of stamen numbers / HO / HE
1 / Aomori, Aomori / 40.82 / 140.76 / 6 / 41(11) / 0.000 / 0.000
2 / Hirosaki, Aomori / 40.60 / 140.46 / 6 / 45(12) / 0.000 / 0.559
3 / Hachinohe, Aomori / 40.51 / 141.53 / 6 / 52(13) / 0.000 / 0.108
4 / Morioka, Iwate / 39.69 / 141.15 / 6 / - / 0.000 / 0.498
5 / Kitakami, Iwate / 39.33 / 141.11 / 5 / 48(12) / 0.000 / 0.000
6 / Ishinomaki, Miyagi / 38.42 / 141.31 / 6 / - / 0.000 / 0.000
7 / Sendai, Miyagi / 38.15 / 140.50 / 5 / 36(11) / 0.019 / 0.019
8 / Shiroishi, Miyagi / 38.02 / 140.63 / 6 / 23(7) / 0.000 / 0.088
9 / Noshiro, Akita / 40.21 / 140.03 / 6 / 47(12) / 0.000 / 0.000
10 / Ohmagari, Akita / 39.49 / 140.45 / 6 / 32(8) / 0.000 / 0.040
11 / Yamagata, Yamagata / 38.22 / 140.32 / 5 / - / 0.019 / 0.567
12 / Oguni, Yamagata / 38.06 / 139.74 / 4 / 40(10) / 0.000 / 0.583
13 / Fukushima, Fukushima / 37.78 / 140.47 / 6 / 36(9) / 0.000 / 0.000
14 / Nishiaizu, Fukushima / 37.58 / 139.65 / 6 / 52(13) / 0.000 / 0.438
15 / Kohriyama, Fukushima / 37.36 / 140.32 / 6 / - / 0.000 / 0.202
16 / Tsukuba, Ibaraki / 36.60 / 140.66 / 5 / - / 0.019 / 0.281
17 / Mito, Ibaraki / 36.39 / 140.42 / 6 / - / 0.000 / 0.343
18 / Tsukuba, Ibaraki / 36.04 / 140.13 / 5 / - / 0.278 / 0.433
19 / Nasu, Tochigi / 37.13 / 140.05 / 6 / - / 0.037 / 0.037
20 / Utsunomiya, Tochigi / 36.54 / 139.81 / 6 / - / 0.037 / 0.367
21 / Nakanojo, Gunma / 36.59 / 138.85 / 18 / 44(11) / 0.000 / 0.071
22 / Kuki, Saitama / 36.08 / 139.65 / 6 / - / 0.000 / 0.000
23 / Chichibu, Saitama / 35.99 / 139.07 / 17 / 46(12) / 0.000 / 0.459
24 / Yokoshibakou, Chiba / 35.67 / 140.50 / 6 / - / 0.000 / 0.397
25 / Chiba, Chiba / 35.61 / 140.11 / 6 / 43(11) / 0.000 / 0.034
26 / Kisaradu, Chiba / 35.36 / 139.92 / 6 / - / 0.000 / 0.000
27 / Tokyo, Tokyo / 35.69 / 139.77 / 6 / 44(11) / 0.000 / 0.034
28 / Hachiohji, Tokyo / 35.65 / 139.33 / 6 / 23(6) / 0.000 / 0.397
29 / Yokohama, Kanagawa / 35.47 / 139.62 / 6 / 22(6) / 0.019 / 0.140
30 / Ebina, Kanagawa / 35.46 / 139.39 / 6 / - / 0.093 / 0.608
31 / Nakajo, Nigata / 38.03 / 139.37 / 5 / 39(10) / 0.022 / 0.481
32 / Nigata, Nigata / 37.92 / 139.05 / 6 / 48(12) / 0.000 / 0.000
33 / Nagaoka, Nigata / 37.45 / 138.86 / 5 / 60(15) / 0.000 / 0.059
34 / Nou, Nigata / 37.10 / 137.99 / 5 / 40(10) / 0.000 / 0.000
35 / Uodu, Toyama / 36.83 / 137.41 / 6 / 39(10) / 0.000 / 0.000
36 / Toyama, Toyama / 36.70 / 137.21 / 6 / 44(11) / 0.000 / 0.114
37 / Wajima, Ishikawa / 37.37 / 136.91 / 5 / - / 0.000 / 0.059
38 / Kanazawa, Ishikawa / 36.59 / 136.62 / 5 / 60(15) / 0.000 / 0.514
39 / Komatsu, Ishikawa / 36.35 / 136.32 / 5 / - / 0.000 / 0.000
40 / Takefu, Fukui / 35.97 / 136.29 / 6 / - / 0.000 / 0.088
41 / Obama, Fukui / 35.50 / 135.74 / 6 / - / 0.000 / 0.000
42 / Kofu, Yamanashi / 35.67 / 138.55 / 18 / 39(10) / 0.000 / 0.296
43 / Kawaguchiko, Yamanashi / 35.50 / 138.76 / 5 / 44(11) / 0.000 / 0.000
44 / Nagano, Nagano / 36.66 / 138.19 / 6 / 47(12) / 0.056 / 0.591
45 / Matsumoto, Nagano / 36.25 / 137.97 / 19 / 43(11) / 0.000 / 0.634
46 / Kisofukushima, Nagano / 35.84 / 137.69 / 5 / 43(11) / 0.000 / 0.415
47 / Takayama, Gifu / 36.14 / 137.26 / 6 / 56(14) / 0.000 / 0.000
48 / Nakatsugawa, Gifu / 35.50 / 137.51 / 6 / 25(9) / 0.019 / 0.019
49 / Tajimi, Gifu / 35.35 / 137.11 / 6 / 37(11) / 0.000 / 0.296
50 / Mishima, Shizuoka / 35.11 / 138.93 / 6 / 36(10) / 0.000 / 0.000
51 / Toyota, Aichi / 35.13 / 137.18 / 6 / - / 0.037 / 0.034
52 / Matsuzaka, Mie / 34.59 / 136.48 / 5 / - / 0.044 / 0.044
53 / Kumano, Mie / 33.94 / 136.09 / 6 / 111(30) / 0.000 / 0.236
54 / Imadu, Shiga / 35.32 / 136.03 / 6 / - / 0.000 / 0.431
55 / Hikone, Shiga / 35.28 / 136.26 / 6 / - / 0.019 / 0.146
56 / Tsuchiyama, Shiga / 34.93 / 136.30 / 6 / 128(32) / 0.019 / 0.426
57 / Ayabe, Kyoto / 35.30 / 135.20 / 6 / - / 0.000 / 0.054
58 / Miyama, Kyoto / 35.28 / 135.59 / 6 / - / 0.019 / 0.079
59 / Kyoto, Kyoto / 35.03 / 135.78 / 6 / - / 0.000 / 0.000
60 / Osaka, Osaka / 34.61 / 135.52 / 6 / - / 0.000 / 0.269
61 / Kumatori, Osaka / 34.41 / 135.44 / 5 / - / 0.000 / 0.494
62 / Nishiwaki, Hyogo / 35.00 / 135.00 / 6 / 132(33) / 0.000 / 0.034
63 / Kobe, Hyogo / 34.51 / 135.11 / 18 / - / 0.000 / 0.497
64 / Gojo, Nara / 34.38 / 135.75 / 6 / - / 0.037 / 0.465
65 / Kamikitayama, Nara / 34.14 / 136.00 / 6 / - / 0.000 / 0.000
66 / Shimizu, Wakayama / 34.09 / 135.41 / 6 / 79(23) / 0.000 / 0.000
67 / Kurisugawa, Wakayama / 33.74 / 135.50 / 6 / - / 0.019 / 0.295
68 / Shingu, Wakayama / 33.72 / 135.97 / 6 / - / 0.000 / 0.000
69 / Tottori, Tottori / 35.51 / 134.20 / 6 / 44(15) / 0.019 / 0.019
70 / Kurayoshi, Tottori / 35.48 / 133.80 / 6 / 56(14) / 0.019 / 0.019
71 / Yonago, Tottori / 35.42 / 133.33 / 6 / 56(14) / 0.019 / 0.019
72 / Izumo, Shimane / 35.33 / 132.72 / 4 / 64(16) / 0.028 / 0.067
73 / Ohda, Shimane / 35.19 / 132.50 / 6 / 56(15) / 0.056 / 0.096
74 / Hamada, Shimane / 34.90 / 132.07 / 5 / 50(13) / 0.000 / 0.079
75 / Tsuyama, Okayama / 35.06 / 134.02 / 6 / 38(13) / 0.000 / 0.034
76 / Niimi, Okayama / 34.94 / 133.51 / 6 / - / 0.000 / 0.000
77 / Shobara, Hiroshima / 34.85 / 133.02 / 5 / 75(19) / 0.089 / 0.099
78 / Oasa, Hiroshima / 34.76 / 132.49 / 5 / 27(8) / 0.000 / 0.000
79 / Kakei, Hiroshima / 34.59 / 132.49 / 6 / - / 0.000 / 0.269
80 / Hiroshima, Hiroshima / 34.45 / 132.41 / 6 / 70(19) / 0.000 / 0.000
81 / Fukuyama, Hiroshima / 34.49 / 133.36 / 5 / 64(17) / 0.000 / 0.000
82 / Yamaguchi, Yamaguchi / 34.17 / 131.46 / 6 / - / 0.000 / 0.034
83 / Shimonoseki, Yamaguchi / 34.05 / 131.01 / 6 / - / 0.000 / 0.000
84 / Anabuki, Tokushima / 34.01 / 134.08 / 6 / - / 0.000 / 0.000
85 / Ikeda, Tokushima / 33.88 / 133.84 / 6 / - / 0.019 / 0.052
86 / Kamihayashi, Ehime / 33.75 / 132.98 / 6 / - / 0.000 / 0.034
87 / Unzendake, Nagasaki / 32.75 / 130.28 / 6 / - / 0.000 / 0.000