Gene name / Access number
(Homo sapiens) / PCR product size
(nucleotides) / Primer sequence
BiP / NM_005347 / 200 / F 5’-ggtgaaagacccctgacaaa-3’
R 5’-gtcaggcgattctggtcatt-3’
C/EBPb / NM_005194 / 126 / F 5’-tttcgaagttgatgcaatcg-3’
R 5’-caacaagcccgtaggaacat-3’
CHOP / NM_004083 / 170 / F 5’-tggaagcctggtatgaggac-3’
R 5’-tgtgacctctgctggttctg-3’
CX3CL1 / NM_002996 / 147 / F5’-tccttaccagcagagcacct-3’
R5’-gtctctgctctgcccatttc-3’
GADD34 / NM_014330 / 171 / F 5’-aggaggctgaagacagtgga-3’
R 5’-ggccatctgcaaattgactt-3’
GRP94 / NM_003299 / 285 / F 5’-agtacggatggtctggcaac-3’
R 5’-tgaggcgaagcattctttct-3’
IL-1b / NM_000576 / 268 / F 5’-cagccaatcttcattgctca-3’
R 5’-gcatcttcctcagcttgtcc-3’
IL-6 / NM_000600 / 272 / F 5’-aggagacttgcctggtgaaa-3’
R 5’-aaagctgcgcagaatgagat-3’
IL-8 / NM_000584 / 180 / F 5’-caggaattgaatgggtttgc-3’
R 5’-aaaccaaggcacagtggaac-3’
IRE1a / NM_001433 / 261 / F 5’-agggagaggagggaatcgta-3’
R 5’-cagtccctaatgccacacct-3’
MCP-1 / NM_002982 / 171 / F 5’-ccccagtcacctgctgttat-3’
R 5’-tggaatcctgaacccacttc-3’
PERK / NM_004836 / 175 / F 5’-caggcaaaggaaggagtctg-3’
R 5’-aacaactccaaagccaccac-3’
RANTES / NM_002985 / 285 / F 5’-gctgcagtgagctgagattg-3’
R 5’-gccagtaagctcctgtgagg-3’
RPL13A / NM_012423 / 124 / F 5’-cctggaggagaagaggaaagaga-3’
R 5’-gaggacctctgtgtatttgtcaa-3’
TNF-a / NM_000594 / 208 / F5’-tccttcagacaccctcaacc-3’
R5’-cagggatcaaagctgtaggc-3’

Supplemental table 1. Primer sequences used for RT-PCR analysis (A, Homo sapiens, B, Rattus norvegicus)

A.


B.

Gene name / Access number
(Rattus norvegicus) / PCR product size
(nucleotides) / Primer sequence
CHOP / NM_024134 / 236 / F 5’-cgacagagccaaaataacagc-3’
R 5’-ggatgcagggtcaagagtagtg-3’
CXCL1 / NM_030845 / 215 / F 5'-gcggagagatgagagtctgg-3’
R 5'-gagacgagaaggagcattgg-3’
CX3CL1 / NM_134455 / 106 / F5’-ctcgtcccagagtgaggaag-3’
R5’-ctgctcctcaggcctacaac-3’
HERP / NM_053523 / 110 / F 5’-agaacttgcggatgaatgc-3’
R 5’-gaggaaaacggaaaatgtcg-3’
Il-1b / NM_031512 / 230 / F 5’-aggcttccttgtgcaagtgt-3’
R 5’-tgagtgacactgccttcctg-3’
Il-6 / NM_012589 / 136 / F 5’-taccccaacttccaatgctc-3’
R 5’-ggtttgccgagtagacctca-3’
MCP-1 / NM_031530 / 192 / F 5’-ccagaaaccagccaactctc-3’
R 5’-ccgactcattgggatcatct-3’
P4HB / NM_012998 / 206 / F 5’-tgaactggctgaagaaacg-3’
R 5’-ggaaaacacatcgctattgg-3’
RANTES / NM_031116 / 122 / F 5’-atatggctcggacaccactc-3’
R 5’-tgacaaagacgactgcaagg-3’
TBP / NM_001004198 / 132 / F 5’-tgcacaggagccaagagtgaa-3’
R 5’-cacatcacagctccccacca-3’
TNF-a / NM_012675 / 211 / F 5’-actcccagaaaagcaagcaa-3’
R 5’-cgagcaggaatgagaagagg-3’


Supplemental table 2. Primary antibodies used for (A) Western blot, (B) immunofluorescence and (C) immunohistochemistry analyses.

A.

Antibody / Dilution / Reference
Anti-phospho-eIF2a / 1:500 / ab321571
Anti-IkBa / 1:1000 / 92422
Anti-GRP78 / 1:1000 / sc-10503
Anti-ATF4 / 1:500 / ab505461
Anti-IRE1a / 1:500 / 32942
Anti-HIF-1a / 1:500 / NB100-4494
Anti-NF-kB p65 / 1:1000 / 47642
Anti-Actin / 1:1000 / A26685

B.

Antibody / Dilution / Reference
Anti-NF-kB p65 / 1:20 / 47642

C.

Antibody / Dilution / Reference
Anti-GRP78 / 1:1000 / sc-10503
Anti-NF-kB p65 / 1:50 / sc-80083

1 Abcam, Cambridge, UK

2 Cell Signaling Technology, Saint-Quentin-en-Yvelines, France

3 Santa Cruz Biotechnology, Le Perray-en-Yvelines, France

4 Novus Biologicals, Cambridge, UK

5 Sigma-Aldrich, Saint-Quentin-Fallavier, France