BIO 101 – Exam #2 Study Guide
1)Chromatin – strands of DNA in nucleus during Interphase
2)Sister chromatids = two half’s of a chromosome
3)Chromatids = 2x # of chromosomes /one half of a replicated chromosome
4)Chromosome = coiled up DNA that passes genes on the DNA to daughter cells when the cell divides
- most visible during mitosis/meiosis
5)Centromere – middle of a sister chromatid
- Divides at onset of Anaphase in Mitosis and Anaphase II in Meiosis
6)Spindle fibers – what pull chromosomes apart during cell division
7)Cleavage furrow – bit of cleavage that animal cells make when dividing
8)Diploid (2n) – cell containing 2 homologous sets of chromosomes, one from each parent
- Reproduces sexually
- What all cells have except egg + sperm
- Double the haploid
9)Haploid(n)– half the Diploid # of genes, is the egg + sperm cells
10)Dyad = 2 chromatid
11)Tetrad = 4 chromatid (Haploid #)
12)Crossing over – when chromosomes exchange segments to rearrange genetic information during Prophase I of Meiosis
13)Homologous chromosomes – 2 chromosomes making up matched pair in a diploid cell
- Same length, centromere position, and staining pattern
- Possess genes for same traits at corresponding loci
- 1 from father, 1 from mother
14) Autosomes – chromosome not involved in determining an organism’s sex
15)Sex chromosomes – determine organism’s sex
16)Gametes – reproductive cells (egg/sperm)
17)Chiasmata – microscopically visible site where crossing over has occurred between chromatids of homologous structures during Prophase I of Meiosis
18)Somatic cell – typical body cell
19)Karyotype – micrographs of the metaphase chromosomes of a cell (nucleus)
20)Karyokinesis – division of nucleus
21)Gametogenesis – creation of gametes within gonads
22)OOgenesis – creation of mature egg cells
23)Spermatogenesis – creation of sperm cells
24)Polar bodies – byproducts of primary/secondary oocyte at each point of meiotic division in oogenesis (not in spermatogenesis)
25)Girls are born with their eggs already made in Prophase I; puberty comes during Metaphase II
26)Prophase is longest phase of Meiosis
27)Klinefelters – XXY (when a male has an extra X chromosome)
28)Metafemale – XXX (when a female has an extra X chromosome)
29)Turners Syndrome – X0 (45 chromosomes in female, where they lack another X chromosome)
30)Not viable – Y0
31)Mitosis – growth/repair
- One diploid parent cell divides to form 2 genetically identical daughter cells
- Used for asexual reproduction in unicellular organisms
- Cell cycle = 90% Interphase, 10% Mitotic phase
- Interphase
- G1–cell grows + carries out normal metabolism; organelles duplicate
- S – DNA replication + chromosome duplication
- G2 – construction of spindle apparatus; chromosomes condense; cell continues growing
- Prophase – nuclear envelope + nucleoli disappear
- Spindle fibers begin to form
- Chromosomes begin to supercoil and are visible for 1st time
- Centrosome head to poles of cell
- Metaphase
- Spindle fibers fully formed
- Attached to all of the kinetochores
- Centrosome are on polar sides of cell
- Anaphase
- Sister chromatids pulled apart at centromere by spindle fibers retracting, resulting in 1 member of each sister chromatid pair moving into a new cell
- Telophase
- 2 daughter cells have clear body
- Spindle fibers start disappearing
- Nucleoli reappears
- Chromosomes uncoil
- Cytokinesis–division of the cell/cytoplasm to form 2 separate daughter cells
- Cleavage furrow forms
32)Meiosis – egg/sperm (sexual cell division)
- One parent cell(2n) divides to form 4 haploid daughter cells which are then processed into gametes
- Meiosis I – homologous chromosomes separate
- InterphaseI – chromosomes duplicates each chromosome has 2 genetically identical sister chromatids
- Prophase I – crossing over occurs tetrad forms as a result of synapsis
- Metaphase I – spindle microtubules attached to a kinetochore
- Anaphase I – sister chromatids remain attached homologous chromosomes separate toward 2 poles of cell
- Telophase I + Cytokenesis – cleavage furrow forms
- Meiosis II – sister chromatids separate during Anaphase II
33)Chemistry/Periodic Table of Elements
- Proton = positive charge, Electron = negative charge, Neutron = neutral charge
- # of rows = # of rings of electrons an element has
- Atomic # = # of protons
- Atomic weight = weight of atom’s protons & neutrons
- 1st ring holds 2 electrons max
- 2nd ring holds 8 electrons max
- 3rd ring holds 18 electrons max
- 4th ring holds 32 electrons max
- Octet rule = outer ring of an atom holds 8 electrons or less
- Electrons are in electron cloud that surrounds nucleus of atom
- Protons & Neutrons are in the nucleus
- An atom is positively charged if it is missing 1 or more electrons, negatively charged if it has 1 or more electrons.
- Negatively atoms are attracted to positively charged atoms and vice versa
- Ions are a charged atom, positive or negative
- Isotopes are atoms with the same # of protons & electrons, but different # of neutrons.
- Ex. Carbon-12, carbon-13, and carbon-14 are 3 isotopes of Carbon with mass #’s 12, 13, and 14, respectively. Carbon’s atomic # is 6 meaning each carbon atom has 6 protons, thus they contain 6, 7, and 8 neutrons, respectively.
- ionic bond – chemical bond resulting from attraction between oppositely charged ions
- covalent bond – strong chemical bond where 2 or more atoms share one or more pairs of outer-shell electrons
- organic chemicals have carbon in covalent linkage
- hydrogen bond – hydrogen atoms holding molecules together
34)Cells
- Characteristics of life
- Nutrients
- Respiration
- Need energy
- Enzymes
- Cell structure
- Homeostasis
- Respond to stimuli, i.e. moisture/temp./taste
- Genetic material
- Reproduction
- Growth
- Waste
- Organelles
- Cytoskeleton – maintenance of cell shape; anchorage for organelles; movement of organelles within cells; cell movement; made up of microtubules, microfilaments, and intermediate filaments
- Flagella & cilia – used for movement; have a 9 + 2 arrangement of microtubules covered by cell’s plasma membrane
- Plasma membrane – consists of phospholipid bilayer with embedded proteins, at boundary of cell, acting as selective barrier to passage of ions/molecules in/out of cell
- Smooth ER – lipid synthesis; detox in liver cells; calcium ion storage
- Rough ER – synthesis of membrane lipids & proteins, secretory proteins, and hydrolytic enzymes; formation of transport vesicles
- Nucleus – DNA replication, RNA synthesis; assembling of ribosomal subunits
- Golgi body – organelle in eukaryotic cells consisting of membranous sacs that modify, store, and ship products of the ER
- Peroxisome – breaks down hydrogen peroxide
- Lysosomes – help digest food, bacteria, and a cell’s damaged organelles for recycling
- Mitochondria – conversion of food to energy (ATP)
- Chloroplasts – conversion of light energy to sugar (plant cells only)
- Ribosomes – protein synthesis
- Cell wall – support & protection; binding of cells in tissues (plant cells only)
- Centrioles – microtubules that are important for cell division in mitosis
- Chromatin – genetic material when a cell is not dividing
- Chromosome – genetic material when a cell is dividing
- Eukaryotic cell – cell that has a membrane-enclosed nucleus (plants/animals/fungi/Protista)
- Prokaryotic cell – cell lacking membrane-enclosed nucleus (bacteria/archaea = like extreme environments)
- Not in animal cells – central vacuole, chloroplast, cell wall, plasmodesmata
- Not in plant cells – lysosomes, centriole, flagellum
- Cytoplasm – contents of eukaryotic cell between plasma membrane & nuclear membrane
- Diffusion – going from area of high concentration to one of low concentration
- Osmosis – diffusion of water through a selectively permeable membrane
- Passive transport – diffusion of a substance across biological membrane w/o energy
- Active transport – movement of a substance across biological membrane against its concentration gradient aided by specific transport proteins and require energy (often as ATP)
- Facilitated diffusion – passage of a substance w/ help of specific transport protein across a biological membrane down its concentration gradient
- Phospholipid bilayer – phosphate head = hydrophilic/water-soluble; fatty acid = hydrophobic/water-insoluble
- Endocytosis – cellular uptake of molecules or particles via formation of new vesicles from plasma membrane
- Phagocytosis – type of endocytosis consisting of cell “eating”
- Pinocytosis – type of endocytosis consisting of cell “drinking”
- Exocytosis – movement of materials out of a cell’s cytoplasm by fusion of vesicles w/ plasma membrane
- Receptor-mediated endocytosis – movement of specific molecules into a cell by inward budding of membrane vesicles, containing proteins w/ receptor sites specific to molecules being taken in
- Isotonic – a solution when surrounding a cell that has no effect on passage of water in/out of the cell
- Hypertonic – more solute outside, water flows out, cell shrinks (crenation)
- Hypotonic – more solute inside, water flows in, cell bursts (lysis)
- Word roots
- Cyto = cell
- Lyso = breakdown
- Some = body
- Endo = inside
- Exo = outside
- Pro = before
- Eu = real/true
- Kary = nucleus
- Chrome = color
- Lipo = fat
- Hyper = too much
- Hypo = too little
35)Scientific Method
- Observations
- Hypotheses
- Predictions
- Experimentation
- Conclusions
36)Metric System
- Used to measure length, weight, volume, and temperature
- Length
- 1,000m = 1km
- 1.6km = 1 mile
- Kilo = 1,000
- 1m = 100 centi (hundredth) = 1,000 milli (thousandth) = 1,000,000 micro (millionth) = 1,000,000,000 nano (billionth) = 10,000,000,000 angstrom (ten billionth)
- 5,280 ft = 1 mile
- 30cm = 1ft
- 2.54cm = 1in
- Weight
- ST (short ton) = 2,000lbs (used in U.S.)
- Long Ton = 2,240lbs (used in ships)
- Metric Ton = 1,000kg = 2,206lbs
- Volume
- 1 barrel of oil = 42 gallons
- 1 gallon = 3.78 liters
- 1 liter = 1.06 quarts
- Temperature
- 0 degrees C = 32 degrees F
- 100 degrees C = 212 degrees F
- 37 degrees C = 98.6 degrees F
- 20 degrees C = 68 degrees F
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