Supplementary Fig.S1Schematic diagram of the T-DNA region of binary plasmidpCAMBIA1301-AtMYB12. LB, left border; RB, right border; hptⅡ,hygromycinphosphotransferaseⅡgene; AtMYB12,ArabidopsisMYB transcription factor gene; gusA, β-glucuronidase gene; 35S, cauliflower mosaic virus (CaMV) 35S promoter; 35S T,CaMV 35S terminator; NOS T,nopaline synthase terminator.

Supplementary Fig.S2PCR analysis ofthe AtMYB12-overexpressing Arabidopsisplants.M, DL2000 DNA marker; W, water as negative control; P, plasmid pCAMBIA1301-AtMYB12 as positive control; WT, negative control; #1, #3, #4, #6, #8, transgenic plants.

Supplementary Fig.S3Expression analysis ofthe AtMYB12 gene in transgenicplants and WT.The Arabidopsisactin gene was used as an internal control. The results are expressed as relative values based on WT as reference sample set to 1.0. Data are presented as means ± SE (n=3). * and ** indicate a significant difference from that of WT at P < 0.05 and < 0.01, respectively, by Student’s t-test.

Supplementary TableS1Primers used in this study

Primer name / Primer sequence (5′-3′)
Primers for chemical synthesis of AtMYB12 gene
AtMYB12-1 / GGA,TCC,ATG,GGA,AGA,GCA,CCA,TGC,TGT,GAG,AAG,GTT,GGC,ATC,AAG,AGA,GGC,AGA,TGG,ACT
AtMYB12-2 / GCT,GAG,GAG,GAC,CAG,ATC,CTC,TCC,AAC,TAC,ATT,CAA,TCC,AAC,GGT,GAA,GGT,TCT,TGG,AGA
AtMYB12-3 / TCC,CTG,CCT,AAG,AAT,GCT,GGC,TTG,AAG,AGA,TGT,GGC,AAG,AGC,TGT,AGA,CTG,AGA,TGG,ATC
AtMYB12-4 / AAC,TAT,CTG,AGA,AGT,GAC,CTG,AAG,AGA,GGC,AAC,ATC,ACT,CCA,GAA,GAA,GAA,GAG,CTG,GTT
AtMYB12-5 / GTC,AAA,CTG,CAT,TCC,ACT,CTT,GGC,AAC,AGA,TGG,TCC,CTG,ATT,GCT,GGT,CAT,CTG,CCA,GGC
AtMYB12-6 / AGA,ACT,GAC,AAC,GAG,ATC,AAG,AAC,TAC,TGG,AAC,TCT,CAC,CTG,AGC,AGA,AAG,CTG,CAC,AAC
AtMYB12-7 / TTC,ATC,AGA,AAG,CCA,TCC,ATC,TCT,CAA,GAC,GTC,TCT,GCT,GTC,ATC,ATG,ACC,AAT,GCC,TCC
AtMYB12-8 / TCT,GCA,CCA,CCA,CCA,CCA,CAG,GCT,AAG,AGA,AGA,CTT,GGC,AGA,ACC,AGT,AGA,AGT,GCC,ATG
AtMYB12-9 / AAA,CCA,AAG,ATC,CAC,AGA,ACC,AAG,ACC,AGA,AAG,ACC,AAG,AAG,ACC,TCT,GCA,CCA,CCT,GAG
AtMYB12-10 / CCT,AAT,GCT,GAT,GTT,GCT,GGT,GCT,GAC,AAA,GAA,GCA,CTG,ATG,GTC,GAG,TCT,AGT,GGA,GCT
AtMYB12-11 / GAG,GCT,GAG,CTT,GGC,AGA,CCA,TGT,GAC,TAC,TAT,GGA,GAT,GAC,TGC,AAC,AAG,AAC,CTG,ATG
AtMYB12-12 / AGC,ATC,AAT,GGT,GAC,AAC,GGA,GTT,CTG,ACC,TTC,GAT,GAC,GAC,ATC,ATC,GAT,CTG,CTG,CTG
AtMYB12-13 / GAT,GAG,TCC,GAT,CCT,GGA,CAC,CTG,TAC,ACC,AAC,ACC,ACC,TGT,GGT,GTC,ATG,GGT,AGC,TGC
AtMYB12-14 / ATC,ACC,GAA,TTC,TAA,GAG,CTC
Primers for subcellular localization of AtMYB12
AtMYB12-SL-F / AAACTAGTATGGGAAGAGCACCATGC
AtMYB12-SL-F / AAGGCGCGCCGAATTCGGTGATGCAGCTA
Primers for constructing expression vector
AtMYB12-OE-F / CGGGATCCATGGGAAGAGCACCATGCTGTGAGA
AtMYB12-OE-R / TTCGAGCTCGAATTCGGTGATGCAGCTACCC
Primers for identifying transformants
hptⅡ-PCR-F / ACAGCGTCTCCGACCTGATGCA
hptⅡ-PCR-R / AGTCAATGACCGCTGTTATGCG
Primers for real-time quantitative PCR
AtMYB12-F / CCAACTACATTCAATCCAACGGT
AtMYB12-R / ACGTCTTGAGAGATGGATGGC
AtPAL-F / ATCAGCAGTGAGTCAGGTGG
AtPAL-R / CTTGAGACATTCCAACAACG
AtCHS-F / CGCATCACCAACAGTGAACAC
AtCHS-R / TCCTCCGTCAGATGCATGTG
AtCHI-F / CCGGTTCATCGATCCTCTTC
AtCHI-R / ATCCCGGTTTCAGGGATACTATC
AtF3H-F / CAGATCGTTGAGGCTTGTGAGA
AtF3H-R / GACGAGTCATATCCGCCACTAAGT
AtF3’H-F / GCTCTCGCCGGAGTATTCAA
AtF3’H-R / CCAGCGACGCCTTGTAAATC
AtFLS-F / CCGTCGTCGATCTAAGCGAT
AtFLS-R / CGTCGGAATCCCGTGGT
AtDFR-F / CTCCTATCACTCGGAACGAGGCGCATTAC
AtDFR-R / TGGTCGGTCCATTCATCACA
AtZEP-F / CGGAGCTTTCTTCTTGATGG
AtZEP-R / TCGATTTCGGAGTTTTCCTG
AtNCED-F / CGCCGGTTTAGTTTATTTCAATGGT
AtNCED-R / AATCGTACCGACCCGAAGTTTCTAA
AtABA2-F / ATTGATCACTGGAGGAGCCACAG
AtABA2-R / ATTACGAATATCAGGGCACGGTG
AtAAO-F / CAACAGCCATGTTGATACCG
AtAAO-R / TCTTTGACCTGCACATCGAG
AtP5CS-F / ATGATCTTATTTATGTTCTGC
AtP5CS-R / CACTATCTTCCGTCACTAT
AtP5CR-F / AGTTTAGCTTCACAGACCGTTC
AtP5CR-R / GCTCTGTGAGAGCTCGCGGCTTC
AtLEA-F / GATTGACCCGGCTGAGCTACGA
AtLEA-R / AGATGGGATTCACCACAAAAGA
AtSOD-F / ATGAGAAGTTCTATGAAGAG
AtSOD-R / GTCTTTATGTAATCTGGT
AtCAT-F / GCAACTACCCCGAGTGGAAA
AtCAT-R / TGTTCAGAACCAAGCGACCA
AtPOD-F / TCCGGGAGCCACACCATTGG
AtPOD-R / TGGTCGGAATTCAACAG
Atactin-F / GCACCCTGTTCTTCTTACCGA
Atactin-R / AGTAAGGTCACGTCCAGCAAGG