Applicant (Last, First, Middle): Wenzlau, Janet M.

BIOGRAPHICAL SKETCH
Provide the following information for ALL key personnel in the order listed in section “Applicant and Key Personnel”.
Follow this format for each person. Duplicate biosketch form as needed. DO NOT EXCEED 2 PAGES per person.

NAME

Janet M. Wenzlau /

POSITION TITLE

Instructor
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)
INSTITUTION AND LOCATION / DEGREE / YEAR(s) / FIELD OF STUDY
Denison University, Granville, OH / BS / 1983 / Biology
Ohio State University, Columbus, OH / PhD / 1989 / Molecular Genetics
University of Texas Southwestern Medical Center, Dallas / Post-doctoral Fellowship / 1990-92

A.  Positions and Honors. List in chronological order previous positions, concluding with your present position. List any honors. Include present membership on any public or private advisory committee.

1983-89 Graduate student/Graduate Research Assistant, Dept of Molecular Genetics, Ohio State University, Columbus, OH

1989-90 Post Doctoral Research Fellow, Dept of Molecular Genetics, Ohio State University, Columbus, OH

1990-92 Post Doctoral Research Fellow, Dept of Molecular Genetics, University of Texas Southwestern Medical Center, Supervisors: Drs. Michael S. Brown and Joseph L. Goldstein

1993-94 Adjunct Asst Professor, Department of Biology, University of Colorado, Colorado Springs

1995 Research Associate, Department of Medical Oncology, University of Colorado Health Sciences Center, Denver, CO Supervisor: Dr. Jeanette Mladenovic

1995-2000 Research Associate, Department of Pathology, University of Colorado Health Sciences Center, Denver, CO Supervisor: Dr. Steven Nordeen

2000-04 Research Associate, Department of Pediatrics, Division of Critical Care, University of Colorado Health Sciences Center, Denver, CO Supervisor: Dr. Mary Weiser-Evans

2004-08 Research Associate, Barbara Davis Center for Childhood Diabetes, Dept of Pediatrics, University of Colorado Health Sciences Center, Denver, CO Supervisor: Dr. John C. Hutton

12/08 Instructor, Dept of Pediatrics, University of Colorado Denver, Aurora, CO

HONORS:

1986 Award winner at the ICSABER Twelfth Annual Forum for Graduate Students in the Biological and Health Sciences

7/87-6/88 Deans's Graduate Research Associate (12 month fellowship) in the College of Biological Sciences, One awarded annually

1988 Winner of the Outstanding Research Award for Biological Sciences at the Council of Graduate Students Research and Scholarly Activities Forum. One awarded annually

1989 Award winner at the ICSABER Fourteenth Annual Forum for Graduate Students in the Biological and Health Sciences

6/90-6/92 Post-doctoral Research Fellow for the Jane Coffin Childs Memorial Fund for Medical Research

B.  Selected peer-reviewed publications (in chronological order). List the title and complete reference to all publications during the past three years and to representative earlier publications pertinent to this application. Do not include publications submitted or in preparation. (Do not exceed this page plus one continuation page. Consecutively number continuation pages for this section.)

Wenzlau, J.M., Saldanha, R.J., Butow, R.A., and Perlman, P.S. (1988). A Latent Intron-Encoded Maturase is Also an Endonuclease Needed for Intron Mobility. Cell 56, 421-430. PMID 2536592

Wenzlau, J.M. and Perlman, P.S. (1990). Mobility of Two Optional G+C-Rich Clusters of the var1 Gene of Yeast Mitochondrial DNA. Genetics 126, 53-62. PMID 2227389

Andres, D.A., Milatovich, A., Ozcelik, T., Wenzlau, J.M., Brown, M.S., Goldstein, J L. and Francke, U. (1993). cDNA Cloning of the Two Subunits of Human CAAX Farnesyltransferase and Chromosomal Mapping of FNTA and FNTB Loci and Related Sequences. Genomics 18 (1), 105-112. PMID 8276393

Wenzlau, J.M., and Berry-Lowe, S. (1995). Nucleotide Sequence of a Gene Encoding Glutamate 1-Semialdehyde Aminotransferase (U10278) from Arabidopsis thaliana “Columbia”. Plant Physiology 108 (3) 1342.

Mourani, P.M., Garl, P.J., Wenzlau, J.M., Stenmark, K.R. Weiser-Evans, M.C.M. (2004). A Unique Highly Proliferative Growth Phenotype Expressed by Embryonic and Neointimal SMC is Driven by Constitutive Akt, mTOR, and p70S6K Signaling and is Actively Repressed by PTEN. Circulation 109(10): 1299-1306. PMID 14993145

Garl, P.J., Wenzlau, J.M., Walker, H.A., Whitelock J.M., Costell, M., and Weiser-Evans, M.C.M. (2004). Perlecan-Induced Suppression of Smooth Muscle Cell Proliferation is Mediated through Increased Activity of the Tumor Suppressor PTEN. Circulation Research 94: 175-183. PMID 14656929

Gerasimovskaya, E.V., Tucker, D.A., Weiser-Evans, M., Wenzlau, J.M., Klemm, D.J., Banks, M., and Stenmark, K.R. (2005). Extracellular ATP-induced proliferation of advential fibroblast requires phphoinositide 3-kinas, Akt, mamamalian target of rapamycin, and p70 S6 kinase signaling pathways. Journal of Biological Chemistry 280: 1838-1848. PMID 15522879

Wenzlau, J.M., Garl P.J., Simpson P., Stenmark, K.R., West J., Artinger, K.B., Nemenoff, R.A., and Weiser-Evans, M.C.M. (2006). Embryonic Growth-Associated Protein is One Subunit of a Novel N-Terminal Acetyltransferase Complex Essential for Embryonic Vascular Development. Circulation Research 98: 846-855.

Wenzlau, J.M., Juhl, K., Yu, L., Moua, O., Sarkar, S., Gottlieb, P., Rewers, M., Eisenbarth, G.S., Jenson, J., Davidson, H.W., and Hutton, J.C. The Cation Efflux Transporter ZnT8 (Slc30A8) is a Major Autoantigen in Human Type I Diabetes. (2007). Proceedings of the National Academy of Science 104(43): 17040-5. PMID 17942684

Wenzlau, J.M., Liu, Y., Yu, L., Moua, O., Fowler, K.T., Rangasamy, S., Walters, J., Eisenbarth, G.S., Davidson, H.W., Hutton, J.C. (2008). A Common Non-synonymous Single Nucleotide Polymorphism in the Slc30A8 Gene Determines ZnT8 Autoantibody Specificity in Type 1 Diabetes. Diabetes 57(10): 2693-7. PMID 18591387

Wenzlau J.M., Hutton, J.C., and Davidson, H.W. (2008). New Antigenic Targets in Type 1 Diabetes. Current Opinions in Endocrinology, Diabetes, and Obesity 15(4): 315-20. PMID 18594270

Kawasaki, E., Uga M., Nakamura, K., Kuriya, G., Satoh, T., Fujishima, K., Ozaki, M., Abiru, N., Yamasaki, H., Wenzlau, J.M., Davidson, H.W., Hutton, J.C., and Eguchi, K. (2008). Association Between Anti-ZnT8 Autoantibody Specificities and Slc30A8 Arg325Trp Variant in Japanese Patients with Type 1 Diabetes. Diabetologia 2008 Dec;51(12):2299-302, PMID 18850084.

Wenzlau, J.M., Moua, O., Sarkar, S.A., Yu, L., Rewers, M., Eisenbarth, G.S., Davidson, H.W., and Hutton, J.C. (2008). Slc30A8 is a Major Target of Humoral Autoimmunity in Type 1 Diabetes and a Predictive Marker in Prediabetes. Annual New York Academy of Science. 2008 Dec;1150:256-259.

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Liu, Y., Wenzlau, J.M., Yu, L., Patel, C., Eisenbarth, G.S., Hutton, J.C., and Davidson, H.W. Conserved Epitopes in the Protein Tyrosine Phosphatase Family of Diabetes Autoantigens. (2008). Annual New York Academy of Science. 2008 Dec;1150:245-257.

Wenzlau, J.M., Moua, O., Liu, Y., Eisenbarth, G.S., Hutton, J.C., and Davidson, H.W. Identification of a Major Humoral Epitope in Slc30A8 (ZnT8). Annual New York Academy of Science. 2008 Dec;1150:252-255.

Wenzlau JM, Frisch LM, Gardner TJ, Sarkar S, Hutton JC, Davidson HW. Novel antigens in type 1 diabetes: the importance of ZnT8. Curr Diab Rep. 2009 Apr;9(2):105-112.

Wenzlau JM, Walter M, Gardner T, Frisch L, Yu L, Eisenbarth G, Ziegler A, Davidson HW, Hutton, JC. Kindetics of zinc transport 8 autoantibodies following clinical onset of type 1 diabetes in humans. Submitted.

Grijse JD, Asanghanwa M, Alrecher N, Goubert P, Vermeulen I, Vand Der Meeren S, Decodhex K, Weets I, Keymeulen B, Lampasona V, Wenzlau J, Hutton JC, Pepeleers D, Gorus FK and the Begian Deabetes Registry. Predictive power of screening for antibodies against IA2(beta) and zinc transporter-8 to select first-degree relatives of type 1 diabetic patients with rapid progression to clinical onset of the disease. Implications for prevention trials. Submitted.