Supplemental Data. Lee et al. (2009). Heat shock protein cognate 70-4 and an E3 ubiquitin ligase, CHIP, mediate plastid-destined precursor degradation through the ubiquitin-26S proteasome system in Arabidopsis.
CLUSTAL X (1.83) multiple sequence alignment
At1g79910 ------
At1g79930 ------
At1g11660 ------
P34932 ------
At3g12580 ------MAGKGEG------
Os11g0703900 ------MAGKGEG------
At5g02500 ------MSGKGEG------
At5g02490 ------MAGKGEG------
At3g09440 ------MAGKGEG------
At1g16030 ------MATKSE------
M59828_hHsp70_ ------MAKA------
P08107_HspA1A_ ------MAKA------
HSPA1B ------MAKA------
At5g28540_Bip_ MARSFGANSTVVLAIIFFGCLFALSSAIEEATKLG------
At4g24280_cpHsc70-1_ -MASSAAQIHVLGGIGFASSSSS--KRNLNGKGGTFMPRSAFFGTRTGPFSTPTSAFLRM
At5g49910_cpHsc70-2_ -MASSAAQIHILGGIGFPTSSSSSSTKNLDNKTNS-IPRSVFFGNRTSPFTTPTSAFLRM
At4g37910_mtHsc70-1_ --MASVALLRSFRRREVQMASVS-AFKSVSANGKN-SMFG-----KLGYLARPFCS----
At5g09590_mtHsc70-2_ --MATAALLRSIRRREVVSSPFS-AYRCLSSSGKA-SLNSSYLGQNFRSFSRAFSS----
At2g32120 ------MAEAAYTVASDSENTGEEKSSSSP------
AF391283_Ssa1_ ------
At1g79910 ------MSVVGFDFGNENCLVAVARQRGIDVVLNDESNRETPAIVC
At1g79930 ------MSVVGFDFGNENCLVAVARQRGIDVVLNDESNRETPAIVC
At1g11660 ------MSVVGFDVGNENCVIAVAKQRGIDVLLNDESNRENPAMVS
P34932 ------MSVVGIDLGFQSCYVAVARAGGIETIANEYSDRCTPACIS
At3g12580 ------PAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVA
Os11g0703900 ------PAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVG
At5g02500 ------PAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVA
At5g02490 ------PAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVA
At3g09440 ------PAIGIDLGTTYSCVGVWQHDRVEIIANDQGNRTTPSYVA
At1g16030 ------KAIGIDLGTTYSCVGVWMNDRVEIIPNDQGNRTTPSYVA
M59828_hHsp70_ ------AAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVA
P08107_HspA1A_ ------AAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVA
HSPA1B ------AAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVA
At5g28540_Bip_ ------SVIGIDLGTTYSCVGVYKNGHVEIIANDQGNRITPSWVG
At4g24280_cpHsc70-1_ GTRNGGGASRYAVGPVRVVNEKVVGIDLGTTNSAVAAMEGGKPTIVTNAEGQRTTPSVVA
At5g49910_cpHsc70-2_ GRRNNN-ASRYTVGPVRVVNEKVVGIDLGTTNSAVAAMEGGKPTIVTNAEGQRTTPSVVA
At4g37910_mtHsc70-1_ ------RPVGNDVIGIDLGTTNSCVSVMEGKTARVIENAEGSRTTPSVVA
At5g09590_mtHsc70-2_ ------KPAGNDVIGIDLGTTNSCVAVMEGKNPKVIENAEGARTTPSVVA
At2g32120 ------SLPEIALGIDIGTSQCSIAVWNGSQVHILRNTRNQKLIKSFVT
AF391283_Ssa1_ ------MASAARLTMMWEEVTCPICLDP---FVEPVSIE
.. : :
At1g79910 FGDK--QRFIGTAGAASTMMNPKNSISQIKRLIGRQFSDPELQRDIKSLPFSVTEGPDGY
At1g79930 FGDK--QRFIGTAGAASTMMNPKNSISQIKRLIGRQFSDPELQRDIKSLPFSVTEGPDGY
At1g11660 FGEK--QRFMGAAAAASATMHPKSTISQLKRLIGRKFREPDVQNDLRLFPFETSEDSDGG
P34932 FGPK--NRSIGAAAKSQVISNAKNTVQGFKRFHGRAFSDPFVEAEKSNLAYDIVQLPTGL
At3g12580 FTDS--ERLIGDAAKNQVAMNPTNTVFDAKRLIGRRYSDPSVQADKSHWPFKVVSGPGEK
Os11g0703900 FTDS--ERLIGDAAKNQVAMNPINTVFDAKRLIGRRFSDASVQSDIKLWPFKVIAGPGDK
At5g02500 FTDS--ERLIGDAAKNQVAMNPVNTVFDAKRLIGRRFSDSSVQSDMKLWPFKIQAGPADK
At5g02490 FTDS--ERLIGDAAKNQVAMNPVNTVFDAKRLIGRRFSDASVQSDRQLWPFTIISGTAEK
At3g09440 FTDS--ERLIGDAAKNQVAMNPINTVFDAKRLIGRRFTDSSVQSDIKLWPFTLKSGPAEK
At1g16030 FTDT--ERLIGDAAKNQVALNPQNTVFDAKRLIGRKFSDPSVQSDILHWPFKVVSGPGEK
M59828_hHsp70_ FTDT--ERLIGDAAKNQVALNPQNTVFDAKRLIGRKFGDPVVQSDMKHWPFQVIND-GDK
P08107_HspA1A_ FTDT--ERLIGDAAKNQVALNPQNTVFDAKRLIGRKFGDPVVQSDMKHWPFQVIND-GDK
HSPA1B FTDT--ERLIGDAAKNQVALNPQNTVFDAKRLIGRKFGDPVVQSDMKHWPFQVIND-GDK
At5g28540_Bip_ FTDS--ERLIGEAAKNQAAVNPERTVFDVKRLIGRKFEDKEVQKDRKLVPYQIVNK-DGK
At4g24280_cpHsc70-1_ YTKSG-DRLVGQIAKRQAVVNPENTFFSVKRFIGRKMN--EVDEESKQVSYRVVRDENNN
At5g49910_cpHsc70-2_ YTKSK-DRLVGQIAKRQAVVNPENTFFSVKRFIGRRMN--EVAEESKQVSYRVIKDENGN
At4g37910_mtHsc70-1_ MNQKG-ELLVGTPAKRQAVTNPTNTIFGSKRLIGRRFDDPQTQKEMKMVPYKIVKAPNGD
At5g09590_mtHsc70-2_ FNTKG-ELLVGTPAKRQAVTNPTNTVSGTKRLIGRKFDDPQTQKEMKMVPYKIVRAPNGD
At2g32120 FKDEVPAGGVSNQLAHEQEMLTGAAIFNMKRLVGRVDTDPVVHAS-KNLPFLVQTLDIGV
AF391283_Ssa1_ CGHSFCQECISQVGKGGGSVCPVCRQRFLLKNLRPNRQLANMVNNLKEISQEAREGTQGE
:. . : . .
At1g79910 PLIHANYL-GEIRAFTPTQVMGMMLSNLKGIAEKNLNTAVVDCCIGIPVYFTDLQRRAVL
At1g79930 PLIHANYL-GEKRAFTPTQVMGMMLSNLKGIAEKNLNTAVVDCCIGIPVYFTDLQRRAVL
At1g11660 IQIRLRYM-GEIQSFSPVQILGMLLSHLKQIAEKSLKTPVSDCVIGIPSYFTNSQRLAYL
P34932 TGIKVTYM-EEERNFTTEQVTAMLLSKLKETAESVLKKPVVDCVVSVPCFYTDAERRSVM
At3g12580 PMIVVNHK-GEEKQFSAEEISSMVLIKMREIAEAFLGSPVKNAVVTVPAYFNDSQRQATK
Os11g0703900 PMIVVQYK-GEEKQFAAEEISSMVLIKMREIAEAYLGTTIKNAVVTVPAYFNDSQRQATK
At5g02500 PMIYVEYK-GEEKEFAAEEISSMVLIKMREIAEAYLGVTIKNAVVTVPAYFNDSQRQATK
At5g02490 PMIVVEYK-GEEKQFAAEEISSMVLIKMREIAEAFLGTTVKNAVVTVPAYFNDSQRQATK
At3g09440 PMIVVNYK-GEDKEFSAEEISSMILIKMREIAEAYLGTTIKNAVVTVPAYFNDSQRQATK
At1g16030 PMIVVSYK-NEEKQFSPEEISSMVLVKMKEVAEAFLGRTVKNAVVTVPAYFNDSQRQATK
M59828_hHsp70_ PKVQVSYK-GETKAFYPEEISSMVLTKMKEIAEAYLGYPVTNAVITVPAYFNDSQRQATK
P08107_HspA1A_ PKVQVSYK-GETKAFYPEEISSMVLTKMKEIAEAYLGYPVTNAVITVPAYFNDSQRQATK
HSPA1B PKVQVSYK-GETKAFYPEEISSMVLTKMKEIAEAYLGYPVTNAVITVPAYFNDSQRQATK
At5g28540_Bip_ PYIQVKIKDGETKVFSPEEISAMILTKMKETAEAYLGKKIKDAVVTVPAYFNDAQRQATK
At4g24280_cpHsc70-1_ VKLECPAI---NKQFAAEEISAQVLRKLVDDASRFLNDKVTKAVITVPAYFNDSQRTATK
At5g49910_cpHsc70-2_ VKLDCPAI---GKQFAAEEISAQVLRKLVDDASRFLNDKVTKAVITVPAYFNDSQRTATK
At4g37910_mtHsc70-1_ AWVEAN-----GQKFSPSQIGANVLTKMKETAEAYLGKSINKAVVTVPAYFNDAQRQATK
At5g09590_mtHsc70-2_ AWVEAN-----GQQYSPSQIGAFILTKMKETAEAYLGKSVTKAVVTVPAYFNDAQRQATK
At2g32120 RPFIAALVNNAWRSTTPEEVLAIFLVELRLMAEAQLKRPVRNVVLTVPVSFSRFQLTRFE
AF391283_Ssa1_ R------CAVHRERLHLFCEKDG------KALCWVCAQSRKHR
. .: .. . : :
At1g79910 DAATIAGLHPLHLIHETTATALAYGIYKTDLPENDQ-----LNVAFIDIGHASMQVCIAG
At1g79930 DAATIAGLHPLRLIHETTATALAYGIYKTDLPESDQ-----LNVAFIDIGHASMQVCIAG
At1g11660 DAAAIAGLRPLRLMHDSTATALGYGIYKTDLVANSSP----TYIVFIDIGHCDTQVCVAS
P34932 DATQIAGLNCLRLMNETTAVALAYGIYKQDLPALEEKP---RNVVFVDMGHSAYQVSVCA
At3g12580 DAGVISGLNVMRIINEPTAAAIAYGLDKKASSVGEK------NVLIFDLGGGTFDVSLLT
Os11g0703900 DAGVIAGLNVMRIINEPTAAAIAYGLDKKATSVGEK------NVLIFDLGGGTFDVSLLT
At5g02500 DAGVIAGLNVMRIINEPTAAAIAYGLDKKATSVGEK------NVLIFDLGGGTFDVSLLT
At5g02490 DAGVIAGLNVLRIINEPTAAAIAYGLDKKATSVGEK------NVLIFDLGGGTFDVSLLT
At3g09440 DAGVIAGLNVMRIINEPTAAAIAYGLDKKATSVGEK------NVLIFDLGGGTFDVSLLT
At1g16030 DAGAISGLNVLRIINEPTAAAIAYGLDKKGTKAGEK------NVLIFDLGGGTFDVSLLT
M59828_hHsp70_ DAGVIAGLNVLRIINEPTAAAIAYGLDR--TGKGER------NVLIFDLGGGTFDVSILT
P08107_HspA1A_ DAGVIAGLNVLRIINEPTAAAIAYGLDR--TGKGER------NVLIFDLGGGTFDVSILT
HSPA1B DAGVIAGLNVLRIINEPTAAAIAYGLDR--TGKGER------NVLIFDLGGGTFDVSILT
At5g28540_Bip_ DAGVIAGLNVARIINEPTAAAIAYGLDK---KGGEK------NILVFDLGGGTFDVSVLT
At4g24280_cpHsc70-1_ DAGRIAGLEVLRIINEPTAASLAYGFDR----KANE------TILVFDLGGGTFDVSVLE
At5g49910_cpHsc70-2_ DAGRIAGLEVLRIINEPTAASLAYGFER----KSNE------TILVFDLGGGTFDVSVLE
At4g37910_mtHsc70-1_ DAGKIAGLDVQRIINEPTAAALSYGMNN----KEG------VIAVFDLGGGTFDVSILE
At5g09590_mtHsc70-2_ DAGRIAGLDVERIINEPTAAALSYGMTN----KEG------LIAVFDLGGGTFDVSVLE
At2g32120 RACAMAGLHVLRLMPEPTAIALLYAQQQQMTTHDNMGSGSERLAVIFNMGAGYCDVAVTA
AF391283_Ssa1_ DHAMVPLEEAAQEYQEKLQVALGELRRK------QELAEKLEVEIAIKRAD
:. : : :: . . : :
At1g79910 FKKGQLKILSHAFDRSLGGRDFDEVLFNHFAAKFKDEYKIDVSQNAKASLRLRATCEKLK
At1g79930 FKKGQLKILSHAFDRSLGGRDFDEVLFNHFAAKFKDEYKIDVSQNAKASLRLRATCEKLK
At1g11660 FESGSMRVRSHAFDRNLGGRDFDEVLFNHFALEFKEKYNIDVYTNTKACVRLRASCEKVK
P34932 FNRGKLKVLATAFDTTLGGRKFDEVLVNHFCEEFGKKYKLDIKSKIRALLRLSQECEKLK
At3g12580 IEEGIFEVKATAGDTHLGGEDFDNRMVNHFVQEFKRKNKKDITGNPRALRRLRTACERAK
Os11g0703900 IEEGIFEVKATAGDTHLGGEDFDNRMVNHFVQEFKRKNKKDITGNPRALRRLRTACERAK
At5g02500 IEEGIFEVKATAGDTHLGGEDFDNRMVNHFVQEFKRKSKKDITGNPRALRRLRTSCERAK
At5g02490 IEEGIFEVKATAGDTHLGGEDFDNRMVNHFVQEFKRKNKQDITGQPRALRRLRTACERAK
At3g09440 IEEGIFEVKATAGDTHLGGEDFDNRMVNHFVQEFKRKNKKDISGNPRALRRLRTACERAK
At1g16030 IEEGVFEVKATAGDTHLGGEDFDNRLVNHFVAEFRRKHKKDIAGNARALRRLRTACERAK
M59828_hHsp70_ IDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRKHKKDISQNKRAVRRLRTACERAK
P08107_HspA1A_ IDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRKHKKDISQNKRAVRRLRTACERAK
HSPA1B IDDGIFEVKATAGDTHLGGEDFDNRLVNHFVEEFKRKHKKDISQNKRAVRRLRTACERAK
At5g28540_Bip_ IDNGVFEVLSTNGDTHLGGEDFDHRVMEYFIKLIKKKHQKDISKDNKALGKLRRECERAK
At4g24280_cpHsc70-1_ VGDGVFEVLSTSGDTHLGGDDFDKRVVDWLAAEFKKDEGIDLLKDKQALQRLTEAAEKAK
At5g49910_cpHsc70-2_ VGDGVFEVLSTSGDTHLGGDDFDKRVVDWLASTFKKDEGIDLLKDKQALQRLTEAAEKAK
At4g37910_mtHsc70-1_ ISSGVFEVKATNGDTFLGGEDFDNTLLEYLVNEFKRSDNIDLTKDNLALQRLREAAEKAK
At5g09590_mtHsc70-2_ ISNGVFEVKATNGDTFLGGEDFDNALLDFLVNEFKTTEGIDLAKDRLALQRLREAAEKAK
At2g32120 TAGGVSQIKALAG-SPIGGEDILQNTIRHIAPPNEE------ASGLLRVAAQDAI
AF391283_Ssa1_ WKKTVETQKSRIHAEFVQQKNFLVEEEQRQLQELEKDEREQLRILGEKEAKLAQQSQALQ
: : .: * .:
At1g79910 KVLSANP-MAPLNIECLMAEKD----VRGVIKREEFEEISIPILERVKRP-LEKALSDAG
At1g79930 KVLSANP-LAPLNIECLMDEKD----VRGVIKREEFEEISIPILERVKRP-LEKALSDAG
At1g11660 KVLSANA-EAQLNIECLMEEKD----VRSFIKREEFEQLSAGLLERLIVP-CQKALADSG
P34932 KLMSANASDLPLSIECFMNDVD----VSGTMNRGKFLEMCNDLLARVEPP-LRSVLEQTK
At3g12580 RTLSSTA-QTTIEIDSLFEGID----FYTTITRARFEELNMDLFRKCMEP-VEKCLRDAK
Os11g0703900 RTLSSTA-QTTIEIDSLYEGID----FYSTITRARFEELNMDLFRKCMEP-VEKCLRDAK
At5g02500 RTLSSTA-QTTIEIDSLYEGID----FYSTITRARFEELNMDLFRKCMEP-VEKCLRDAK
At5g02490 RTLSSTA-QTTIEIDSLYGGAD----FYSPITRARFEEMNMDLFRKCMEP-VEKCLRDAK
At3g09440 RTLSSTA-QTTIEIDSLFDGID----FYAPITRARFEELNIDLFRKCMEP-VEKCLRDAK
At1g16030 RTLSSTA-QTTIEIDSLHEGID----FYATISRARFEEMNMDLFRKCMDP-VEKVLKDAK
M59828_hHsp70_ RTLSSST-QASLEIDSLFEGID----FYTSITRARFEELCSDLFRSTLEP-VEKALRDAK
P08107_HspA1A_ RTLSSST-QASLEIDSLFEGID----FYTSITRARFEELCSDLFRSTLEP-VEKALRDAK
HSPA1B RTLSSST-QASLEIDSLFEGID----FYTSITRARFEELCSDLFRSTLEP-VEKALRDAK
At5g28540_Bip_ RALSSQH-QVRVEIESLFDGVD----FSEPLTRARFEELNNDLFRKTMGP-VKKAMDDAG
At4g24280_cpHsc70-1_ IELSSLT-QTNMSLPFITATADGPKHIETTLTRAKFEELCSDLLDRVRTP-VENSLRDAK
At5g49910_cpHsc70-2_ IELSSLT-QTNMSLPFITATADGPKHIETTLTRGKFEELCSDLLDRVRTP-VENSLRDAK
At4g37910_mtHsc70-1_ IELSSTT-QTEINLPFITADASGAKHLNITLTRSKFEGLVGKLIERTRSP-CQNCLKDAG
At5g09590_mtHsc70-2_ IELSSTS-QTEINLPFITADASGAKHFNITLTRSRFETLVNHLIERTRDP-CKNCLKDAG
At2g32120 HRLTDQE---NVQIEVDLGNGN---KISKVLDRLEFEEVNQKVFEECERL-VVQCLRDAR
AF391283_Ssa1_ ELISELDRRCHSSALELLQEVIIVLERSESWNLKDLDITSPELRSVCHVPGLKKMLRTCA
:: . : : . :
At1g79910 LTVEDVHMVEVVGSGSRVPAMIKILTEFF-GKEPRRTMNASECVSRGCALQCAILSP--T
At1g79930 LTVEDVHMVEVIGSGSRVPAMIKILTEFF-GKEPRRTMNASECVSRGCALQCAILSP--T
At1g11660 LSLDQIHSVELVGSGSRIPAISKMLSSLF-KRELGRTVNASECVARGCALQCAMLSP--V
P34932 LKKEDIYAVEIVGGATRIPAVKEKISKFF-GKELSTTLNADEAVTRGCALQCAILSP--A
At3g12580 MDKSSVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGN
Os11g0703900 MDKSSVHDVVLVGGSTRIPRVQQLLQDFFNGKELCKNINPDEAVAYGAAVQAAILSGEGN
At5g02500 MDKSTVHDVVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQGAILSGEGN
At5g02490 MDKSTVHEIVLVGGSTRIPKVQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGN
At3g09440 MDKNSIDDVVLVGGSTRIPKVQQLLVDFFNGKELCKSINPDEAVAYGAAVQAAILSGEGN
At1g16030 LDKSSVHDVVLVGGSTRIPKIQQLLQDFFNGKELCKSINPDEAVAYGAAVQAAILTGEGS
M59828_hHsp70_ LDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKS
P08107_HspA1A_ LDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKS
HSPA1B LDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKS
At5g28540_Bip_ LQKSQIDEIVLVGGSTRIPKVQQLLKDFFEGKEPNKGVNPDEAVAYGAAVQGGILSGEGG
At4g24280_cpHsc70-1_ LSFKDIDEVILVGGSTRIPAVQELVRKVT-GKEPNVTVNPDEVVALGAAVQAGVLAG---
At5g49910_cpHsc70-2_ LSFKDIDEVILVGGSTRIPAVQDLVRKLT-GKEPNVSVNPDEVVALGAAVQAGVLSG---
At4g37910_mtHsc70-1_ VTIKEVDEVLLVGGMTRVPKVQEIVSEIF-GKSPCKGVNPDEAVAMGAAIQGGILRG---
At5g09590_mtHsc70-2_ ISAKEVDEVLLVGGMTRVPKVQSIVAEIF-GKSPSKGVNPDEAVAMGAALQGGILRG---
At2g32120 VNGGDIDDLIMVGGCSYIPKVRTIIKNVCKKDEIYKGVNPLEAAVRGAALEGAVTSGIHD
AF391283_Ssa1_ VHIT----LDPDTANPWLILSEDRRQVRLGDTQQSIPGNEERFDSYPMVLGAQHFHSG--
: : . . : . * . .:
At1g79910 -FKVREFQVHESFPFSISLAWKGAATDAQNGGTENQQSTIVFPKGNPIPSVK--ALTFYR
At1g79930 -FKVREFQVHESFPFSISLAWKGAASEAQNGGAENQQSTIVFPKGNPIPSVK--ALTFYR
At1g11660 -FRVRDYEVQDSYPFAIGFSSD------KGPINTPSNELLFPKGQIFPSVK--VLTLHR
P34932 -FKVREFSITDVVPYPISLRWN------SPAEEGSSDCEVFSKNHAAPFSK--VLTFYR
At3g12580 -EKVQDLLLLDVTPLSLGLETAG------GVMTVLIPRNTTIPTKKEQIFSTYS
Os11g0703900 -EKVQDLLLLDVTPLSLGLETAG------GVMTVLIPRNTTIPTKKEQVFSTYS
At5g02500 -EKVQDLLLLDVTPLSLGLETAG------GVMTTLIPRNTTIPTKKEQVFSTYS
At5g02490 -EKVQDLLLLDVTPLSLGLETAG------GVMTTLIQRNTTIPTKKEQVFSTYS
At3g09440 -EKVQDLLLLDVTPLSLGLETAG------GVMTVLIQRNTTIPTKKEQVFSTYS
At1g16030 -EKVQDLLLLDVAPLSLGLETAG------GVMTVLIPRNTTVPCKKEQVFSTYA
M59828_hHsp70_ -ENVQDLLLLDVAPLSLGLETAG------GVMTALIKRNSTIPTKQTQIFTTYS
P08107_HspA1A_ -ENVQDLLLLDVAPLSLGLETAG------GVMTALIKRNSTIPTKQTQIFTTYS
HSPA1B -ENVQDLLLLDVAPLSLGLETAG------GVMTALIKRNSTIPTKQTQIFTTYS
At5g28540_Bip_ -DETKDILLLDVAPLTLGIETVG------GVMTKLIPRNTVIPTKKSQVFTTYQ
At4g24280_cpHsc70-1_ --DVSDIVLLDVTPLSIGLETLG------GVMTKIIPRNTTLPTSKSEVFSTAA
At5g49910_cpHsc70-2_ --DVSDIVLLDVTPLSLGLETLG------GVMTKIIPRNTTLPTSKSEVFSTAA
At4g37910_mtHsc70-1_ --DVKDLLLLDVVPLSLGIETLG------AVFTKLIPRNTTIPTKKSQVFSTAA
At5g09590_mtHsc70-2_ --DVKELLLLDVTPLSLGIETLG------GVFTRLITRNTTIPTKKSQVFSTAA
At2g32120 PFGSLDLLTIQATPLAVGVRANG------NKFIPVIPRNTMVPARKDLFFTTVQ
AF391283_Ssa1_ -KHYWEVDVTGKEAWDLGVCRDS------VRRKGHFLLSSKSGFWTIWL
: . :.. : :. : :
At1g79910 SGTFSIDVQYSDVNDLQ--APPKISTYTIGPFQ-SSKGERAKLKVKVRLNLHGIVSVESA
At1g79930 SGTFSVDVQYSDVNDLQ--APPKISTYTIGPFQ-SSKGERAKLKVKVRLNLHGIVSVESA
At1g11660 ENTFQLEAFYANHNELSPDIPTQISSFMIGPFH-ISHGEAARVKVRVQLNLHGIVTIDSA
P34932 KEPFTLEAYYSSPQDLP-YPDPAIAQFSVQKVTPQSDGSSSKVKVKVRVNVHGIFSVSSA
At3g12580 DNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPRG-VPQITVCFDIDANGILNVSAE
Os11g0703900 DNQPGVLIQVYEGERTRTRDNNLLGKFELSGIPPAPRG-VPQITVCFDIDANGILNVSAE
At5g02500 DNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPRG-VPQITVCFDIDANGILNVSAE
At5g02490 DNQPGVLIQVFEGERARTKDNNLLGKFELSGIPPAPRG-VPQITVCFDIDANGILNVSAE
At3g09440 DNQPGVLIQVYEGERARTKDNNLLGKFELSGIPPAPRG-VPQITVCFDIDANGILNVSAE
At1g16030 DNQPGVLIQVYEGERARTRDNNLLGTFELKGIPPAPRG-VPQINVCFDIDANGILNVSAE
M59828_hHsp70_ DNQPGVLIQVYEGERAMTKDNNLLGRFELSGIPPAPRG-VPQIEVTFDIDANGILNVTAT
P08107_HspA1A_ DNQPGVLIQVYEGERAMTKDNNLLGRFELSGIPPAPRG-VPQIEVTFDIDANGILNVTAT
HSPA1B DNQPGVLIQVYEGERAMTKDNNLLGRFELSGIPPAPRG-VPQIEVTFDIDANGILNVTAT
At5g28540_Bip_ DQQTTVSIQVFEGERSLTKDCRLLGKFDLNGIPPAPRG-TPQIEVTFEVDANGILNVKAE
At4g24280_cpHsc70-1_ DGQTSVEINVLQGEREFVRDNKSLGSFRLDGIPPAPRG-VPQIEVKFDIDANGILSVSAV
At5g49910_cpHsc70-2_ DGQTSVEINVLQGEREFVRDNKSIGSFRLDGIPPAPRG-VPQIEVKFDIDANGILSVSAS
At4g37910_mtHsc70-1_ DNQMQVGIKVLQGEREMAADNKVLGEFDLVGIPPAPRG-MPQIEVTFDIDANGITTVSAK
At5g09590_mtHsc70-2_ DNQTQVGIRVLQGEREMATDNKLLGEFDLVGIPPSPRG-VPQIEVTFDIDANGIVTVSAK
At2g32120 DNQKEALIIIYEGEGETVEENHLLGYFKLVGIPPAPKG-VPEINVCMDIDASNALRVFAA
AF391283_Ssa1_ WNK------QKYEAGTYPQTPLHLQVPPCQVGIFLDYEAGMVSFYNITDH
. : . : : . . :
At1g79910 TLLEEEEVEVSVTKDQSEETAKMDTDKASAEAAPASGDSDVNMQDAKDTSDATGTDNGVP
At1g79930 TLLEEEEVEVPVTKEHSEETTKMDSDKASAEAAPASGDCDVNMQDAKDTSDATGTDNGVP
At1g11660 TLIEYHKENITSEEMISEENHQSSAMKD-GSLDPSSG------
P34932 SLVEVHKSEENEEPMETDQNAKEEEKMQVDQEEPHVEE---QQQQTPAENKAESEEMETS
At3g12580 DKTTGQKNKITITNDK------
Os11g0703900 DKTTGQKNKITITNDK------
At5g02500 DKTTGQKNKITITNDK------
At5g02490 DKTTGKKNKITITNDK------
At3g09440 DKTTGQKNKITITNDK------
At1g16030 DKTAGVKNQITITNDK------
M59828_hHsp70_ DKSTGKANKITITNDK------
P08107_HspA1A_ DKSTGKANKITITNDK------
HSPA1B DKSTGKASKITITNDK------
At5g28540_Bip_ DKASGKSEKITITNEK------
At4g24280_cpHsc70-1_ DKGTGKKQDITITGA------
At5g49910_cpHsc70-2_ DKGTGKKQDITITGA------
At4g37910_mtHsc70-1_ DKATGKEQNITIRSS------
At5g09590_mtHsc70-2_ DKTTGKVQQITIRSS------
At2g32120 VLMPGS------
AF391283_Ssa1_ GSLIYSFSECAFTGPL------
At1g79910 ESAEKPVQMETDSKAKAPKKKVKKTNVPLSELVYGALKTVEVEKAVEKEFEMALQDRVME
At1g79930 ESAEKPVQMETDSKAEAPKKKVKKTNVPLSELVYGALKTVEVEKAVEKEFEMALQDRVME
At1g11660 ------SIGNEPK-AIKRMEIPVVANVSGALTKDELSEAKQRENSLVEQDLKME
P34932 QAGSKDKKMDQPPQAKKAKVKTSTVDLPIENQLLWQIDREMLNLYIENEGKMIMQDKLEK
At3g12580 ------GRLSKEEIEKMVQEAEKYKAEDEEHK
Os11g0703900 ------GRLSKEEIEKMVQEAEKYKSEDEEHK
At5g02500 ------GRLSKDEIEKMVQEAEKYKSEDEEHK
At5g02490 ------GRLSKEDIEKMVQEAEKYKSEDEEHK
At3g09440 ------GRLSKDEIEKMVQEAEKYKSEDEEHK
At1g16030 ------GRLSKEEIEKMVQDAEKYKAEDEQVK
M59828_hHsp70_ ------GRLSKEEIERMVQEAEKYKAEDEVQR
P08107_HspA1A_ ------GRLSKEEIERMVQEAEKYKAEDEVQR
HSPA1B ------GRLSKEEIERMVQEAEKYKAEDEVQR
At5g28540_Bip_ ------GRLSQEEIDRMVKEAEEFAEEDKKVK
At4g24280_cpHsc70-1_ ------STLPKDEVDQMVQEAERFAKDDKEKR
At5g49910_cpHsc70-2_ ------STLPKDEVDTMVQEAERFAKEDKEKR
At4g37910_mtHsc70-1_ ------GGLSDDEINRMVKEAELNAQKDQEKK
At5g09590_mtHsc70-2_ ------GGLSEDDIQKMVREAELHAQKDKERK
At2g32120 ------SSPVVPVIEVRMPTVDDGHGWCA
AF391283_Ssa1_ ------RPFFSPGFNDGGKNTAPLTLCP
.
At1g79910 ETKDRKNAVESYVYDMRNKLSD--KYQN-ISLTRR------GK
At1g79930 ETKDRKNAVESYVYDMRNKLSD--KYQEYITDSEREAFLANLQEVEDWLYEDGEDETKGV
At1g11660 STKDKKNALESFVYEMRDKMLN--TYRNTATESERECIARNLQETEEWLYEDGDDESENA
P34932 ERNDAKNAVREYVYEMRDKLSG--EYEKFVSEDGRNSFTLKLEDTENWLYEDGEDQPKQV
At3g12580 KKVDAKNALENYAYNMRNTIKD-EKIASKLDAADKKKIEDAIDQAIEWLDGNQLAEADEF
Os11g0703900 KKVESKNALENYAYNMRNTIKD-EKIASKLPAADKKKIEDAIDQAIQWLDGNQLAEADEF
At5g02500 KKVEAKNALENYAYNMRNTIQD-EKIGEKLPAADKKKIEDSIEQAIQWLEGNQLAEADEF
At5g02490 KKVEAKNALENYAYNMRNTIRD-EKIGEKLPAADKKKVEDSIEEAIQWLDGNQLGEADEF
At3g09440 KKVDAKNALENYAYNMRNTIRD-EKIGEKLAGDDKKKIEDSIEAAIEWLEANQLAECDEF
At1g16030 KKVEAKNSLENYAYNMRNTIKD-EKLAQKLTQEDKQKIEKAIDETIEWIEGNQLAEVDEF
M59828_hHsp70_ ERVSAKNALESYAFNMKSAVED-EGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEF
P08107_HspA1A_ ERVSAKNALESYAFNMKSAVED-EGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEF
HSPA1B ERVSAKNALESYAFNMKSAVED-EGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEF
At5g28540_Bip_ EKIDARNALETYVYNMKNQVNDKDKLADKLEGDEKEKIEAATKEALEWLDENQNSEKEEY
At4g24280_cpHsc70-1_ DAIDTKNQADSVVYQTEKQLKE---LGEKIPGEVKEKVEAKLQELKDKIGSGSTQEIKDA
At5g49910_cpHsc70-2_ DAIDTKNQADSVVYQTEKQLKE---LGEKIPGPVKEKVEAKLQELKEKIASGSTQEIKDT
At4g37910_mtHsc70-1_ QLIDLRNSADTTIYSVEKSLSE---YREKIPAEIASEIETAVSDLRTAMAGEDVEDIKAK
At5g09590_mtHsc70-2_ ELIDTKNTADTTIYSIEKSLGE---YREKIPSEIAKEIEDAVADLRSASSGDDLNEIKAK
At2g32120 QALNVKYGATLDLITLQRKM------
AF391283_Ssa1_ LNIGSQGSTDY------
:
At1g79910 HSWRICRKLRIG------CMKMGKMRPKV-FMLQSSRSS------R
At1g79930 YVAKLEELKKVGDPVEVRYKESLERGSVIDQLGYCINSYREAAMSTDPK---FDHIELAE
At1g11660 YIEKLNDVKKLIDPIENRFKDGEERVQASKDLLKTIADNRMAAESLPP------PR
P34932 YVDKLAELKNLGQPIKIRFQESEERPKLFEELGKQIQQYMKIISSFKNKEDQYDHLDAAD
At3g12580 EDKM-KELESLCNPIIARMYQG------
Os11g0703900 DDKM-KELEGICNPIIAKMYQG------
At5g02500 EDKM-KELESICNPIIAKMYQG-AG------
At5g02490 EDKM-KELESVCNPIIAKMYQGGAG------
At3g09440 EDKM-KELESICNPIIAKMYQG------
At1g16030 EYKL-KELEGICNPIISKMYQG------
M59828_hHsp70_ EHKR-KELEQVCNPIISGLYQG------
P08107_HspA1A_ EHKR-KELEQVCNPIISGLYQG------
HSPA1B EHKR-KELEQVCNPIISGLYQG------
At5g28540_Bip_ DEKL-KEVEAVCNPIITAVYQR------
At4g24280_cpHsc70-1_ MAALNQEVMQIGQSLYNQPGAGGPG------
At5g49910_cpHsc70-2_ MAALNQEVMQIGQSLYNQPQPGG------
At4g37910_mtHsc70-1_ VEAANKAVSKIGEHMS------
At5g09590_mtHsc70-2_ IEAANKAVSKIGEHMS------
At2g32120 ------
AF391283_Ssa1_ ------
At1g79910 RWVTLLKCVTRSRWKEGR-----SLISLAT--VLTATERQLCLMIPSLITLNWQRSKRF-
At1g79930 KQKVLNECVEAEAWLRGKQQQQDTLPKYATPALLSADVKSKAEALDKFCRPIMTKPKPVA
At1g11660 KNAVLDECHKAERWLHEKTTEQESLPKDANPELQSAEIRRKADALNATCKYIGKSNSPPA
P34932 MTKVEKSTNEAMEWMNNKLNLQNKQSLTMDPVVKSKEIEAKIKELTSTCSPIISKPKPKV
At3g12580 -----AGPDMGGAGGMDDDTPAGGSGGAG-PKIEEVD------
Os11g0703900 -----AGADMAGGMDEDDAPPAGGSG-AG-PKIEEVD------
At5g02500 -----GEAGGPGASGMDDD-APPASGGAG-PKIEEVD------
At5g02490 -----GEAGGPGASGMDEDEAPPASGGAG-PKIEEVD------
At3g09440 -----GEAGGPAAGGMDED-VPPSAGGAG-PKIEEVD------
At1g16030 ------GAAAGGMPTDGDFSSSGAAGGPKIEEVD------
M59828_hHsp70_ ------AGGPGPGGFGAQGPKGGSG-SG-PTIEEVD------
P08107_HspA1A_ ------AGGPGPGGFGAQGPKGGSG-SG-PTIEEVD------
HSPA1B ------AGGPGPGGFGAQGPKGGSG-SG-PTIEEVD------
At5g28540_Bip_ ------SGGAPGGAGGESSTEEEDESH----DEL------
At4g24280_cpHsc70-1_ -----AGPSPGGEGASSGD-SSSSKGGD-GDDVIDADFTDSQ------
At5g49910_cpHsc70-2_ -----ADSPPGGEASSSSDTSSSAKGGDNGGDVIDADFTDSN------
At4g37910_mtHsc70-1_ ------KGSGSSGSD-GSSGEGTSGTEQTPEAEFEEASGSRK------
At5g09590_mtHsc70-2_ ------GGSGGGSAP-GGGSEG--GSDQAPEAEYEEVKK------
At2g32120 ------
AF391283_Ssa1_ ------
At1g79910 ------
At1g79930 KAEAPQAKGGEQADEGKSEPEQPASAEPMETENPAEGST------
At1g11660 KPEHNGSYGSRKSDDMELD------
P34932 EPPKEEQKNAEQNGPVDGQGDNPGPQAAEQGTDTAVPSDSDKKLPEMDID
At3g12580 ------
Os11g0703900 ------
At5g02500 ------
At5g02490 ------
At3g09440 ------
At1g16030 ------
M59828_hHsp70_ ------
P08107_HspA1A_ ------
HSPA1B ------
At5g28540_Bip_ ------
At4g24280_cpHsc70-1_ ------
At5g49910_cpHsc70-2_ ------
At4g37910_mtHsc70-1_ ------
At5g09590_mtHsc70-2_ ------
At2g32120 ------
AF391283_Ssa1_ ------