(E)T-1 Genes analyzed for mutations, PCR primer sequences and annealing temperatures (Tm).
GENE / Exon / Forward/Reverse primers / Tm
2_19 / 1 / ccagagtcctccatttccaa
cacccaaacctgtccttcc / 62
2,3 / attctggggttcgctggtat
caggcagggatgtcacaaag / 65
4-6 / taccatgtgtgctggtcctg
gagcagtgtctcaggggaag / 67.5
7-9 / gtgctcagtggtgcctgat
gtgtgagcctcagcctctgt / 60
BGN / 1 / acaaactgcccaggagtgagta
gcagagcaaggtcagaagggag / 60
2 / ctgatgatcccctcgcctcttc
cagcatgtgcccccttcagaag / 60
3 / gtcagtgtccctgtgtgtgtgtccc
cacactggctacaatgaaatccgtt / 60
4 / tgggtttgagcaaccacagctg
cagtcttctctttagccgtggtgat / 60
5 / gatcaccacggctaaagagaagact
ctgttgagcctttccctgagtagat / 60
6 / cgatctactcagggaaaggctcaac
ggactcaaaggctggaggtaaggat / 60
7 / cagagtccctcagtgctgtgtg
ttagggaacctggctgtgcatg / 60
8, first half / cctcacaccaccaaacacacctctaaggctgatgccgttgtagtagg / 60
8, 2nd half / gtgtggcccttgaagtaggttg
aaggtctgaggaagcacaag / 60
CLIC2 / 1 / tttctgcgtcacacctcttg
gcccaaatgtggagaattttt / 58
2 / agcaactggctcatttccta
ggtacctggggctaagtcct / 62
3 / tgaccaggtaagagaaatcagga
aggcccagaaatccaaaagt / 58
4 / caagctagggcacctggtc
actatgccagtccccaaaat / 58
5 / ggtgtctaaatgtgtggaaagc
ccgcactggaaggtcactat / 58
6 / tgcaaaaacctttctaatatgtcaa
ttgtgtcgataagatgcctttg / 58
DNL1L / 1 / gtaaggtggttcatgtactc
aagggacctcggtccagtac / 58
2 / ctcaggaacacagcctcttc
tatcagccttggtgggagcc / 60
3 / tcactgggaggctggtgc
ttggctcatggtggttaggc / 60
4, 5 / cagtcagtgagccttaacac
ctaatgagcctacctcac / 56
6, 7 / agcgccgtttggcacttg
tatccttgtccctcctgtac / 56
8, first half / ttgtacaggagggacaagg
ttccctgtgaccctgctg / 62
8, 2nd half and 9 / gcacccactgcacctatg
cctgactgcattgttcac / 56
FLNA / 2 / gaagggggtggtttggag
ctagtgggggcattccaac / 62
3, 4 / ctccgagtctctcccaactg
agaccctgaccccaaagc / 65
5 / gccacgtttagatgggacac
atcggcagttgggagagact / 60
6- 8 / gctctccccacagaccag
ggaatgtgcctgacttaggg / 60
9, 10 / tctccctctgccaagacaag
gatctttacggcaggtgagg / 65
11, 12 / gtccttgccatcgtctgtc
gtgaagggccccagtaagtt / 60
13 / cagggacacagagtgccatc
gacagacgatggcaaggac / 62
14, 15 / gtgtgccacaaccacttgaa
tgcctgggttgtagctctg / 60
16-18 / gaggaacacacagggaccat
gctgagggagcttctgagtc / 60
19, 20 / atccctggatgtgacaaagg
atcaagggtaggagggcttg / 67
21 / agggcaggtctggagaagat
aaggcctttgtcacatccag / 60
22 / acctgcaaaggcacagagag
tggaaagtcaagcaggaggt / 62
23, 24 / aaacagacagccggtcattc
gggacagggaccagaacc / 62
25, 26 / ctgtcacctgaggctcttcc
caacaagcccaacaagttca / 62
27, 28 / ccctccttggtgcagctc
ccaggagactagctgatgc / 62
29, 30 / ctggaactgtcctgggaatc
agactgtccctgtagcagca / 62
31-33 / gaggtcagggcagctcatt
gacgggaacaacctgaacc / 60
34, 35 / tctcctgagtccagccctta
agccaaggaggccagact / 67
36, 37 / cgaatgaaatccctggacac
agtgcaggccaactggag / 62
38, 39 / cccctccagtcacatactgc
cagggggttcagaaaggag / 62
40, 41 / ctttcctccacccccaac
gcccacaggcagtatgtgac / 65
42, 43 / gggggtcactgaacacagg
caggcatgtctaccttggcta / 62
44 / ttgtactctcagcctgcttcc
cctttccgtcctcccttg / 65
45 / gaggcaagggcctagcag
gacaccatctgctggtttga / 56
46 / gctaagaggtggctgtggtg
aggtgacaagcctgtgctg / 71
47 / ctcattttggtgggaaggtg
tcaaggccaaagtcacaggt / 58
48 / ggggttgaggggaagagg
agaggagagcaggacaccag / 67
(E)T-1 Genes analyzed for mutations, PCR primer sequences and annealing temperatures (Tm) (continued)
GENE / Exon / Forward/Reverse primers / Tm
GDX / 1- 4 / agtggacgccgtcgcgac
aacacacttagtgcgacatgc / 60
HSPRY3 / 2 / gccaccctgacttaaaacca
tatcactcctccccctctcc / 63
IRAK1 / 1 , 2 / caaggaaggaaaggaaggttg
tcgaaggggaagtgagtcag / 60
3 , 4 / tccttcctccttgtctcgaa
accttcctttccttccttgg / 56
5 / aaaggctggagaagtggtga
cttgggcagaagggaagag / 60
6 , 7 / aggtgtcaggagtgctttgg
ttcgcttcgagagcctca / 62
8 , 9 / ctgtggcttctcctcctctg
gatggttgggatcaaggaac / 60
10 / cagctgtgtgtccctcctg
caatccatgtccacaccaga / 65
11 / gaatgcgggctttctatctg
gccacctggtagccatactg / 65
12 / agaggccttggtccctctc
ccatctcaccctgagagtcc / 65
13 , 14 / actttgacttccggatttgg
cagcatgaggctaagggagt / 58
P3 / 2, first
half / ccatcaaggaagacagca
atgagccccttcagaacctc / 62
2, 2nd
half / ctgcatcaaggtctcacctg
gcctggatttttctgagtgc / 67.5
SYBL1 / see reference 23
TMG3 / 1,2 / ggtttcactgcaactttgctt
cagggcctgttcttgctatt / 62
3 / ctctgagcccacctggag
taggagacctcggaaagagg / 67.5
ZNF185 / 4 , 5 / cctctggctacaagatgacca
caaaaggcaccattccatta / 60
6 , 7 / tggcacccatcagatgctc
taacaggcctccaaggtgtc / 60
8 / ctctagggcagccaagaaga
tgactgagtggcaggtatgg / 60
9 / tttctcacctgcaacccact
ggctgctgcttccatctcta / 62
10 / ccagccttctttggtctgag
ctctccaccccagaagttca / 60
11 / ggctgaggcagggttagttt
aacaaaaacatgggctccag / 60
12 / gtctcagggcactcacttca
ctgccctggacaaaaacact / 60
13 / gtgtcagctcccaggttgac
agtggcggaggaacatctc / 60
14 / ttccctggaatcttctggtg
gagaaggccgacatggaag / 58
15 / ccctcctctcccctatcaaa
cccttccctgattctcctct / 58
16, 17 / tctgtacaccagcctgacca
tgagtctcttggccacttgtc / 70