Supplementary Table S1.Pairwise similarities of 16SrRNA, gyrB and rpoD genes between strain NEAU-ST5-5 and type strains of the closely related species.

Type strains / Pairwise similarity (%)
16SrRNA / gyrB / rpoD
Pseudomonas stutzeriATCC17588T / 98.8 (CP002881) / 87.2 (AB039393) / 89.3 (AJ631316)
Pseudomonas xanthomarinaKMM1447T / 98.7 (AB176954) / 83.2 (AM905836) / 81.3 ( AM905872)
Pseudomonas kunmingensisHL22-2T / 98.6 (JQ246444) / 90.5 (KP151496) / 90.4 (KP151500)
Pseudomonas alcaliphilaAL15-21T / 98.1 (AB030583) / 82.5 (FN554167) / 76.6 (FN554448)
Pseudomonas oleovoranssubsp.lubricantisRS1T / 98.1 (DQ842018) / 81.3 (EF675630) / 78.1 (EF667505)
Pseudomonas plecoglossicidaFPC951T / 97.9 (AB009457) / 82.9 (AB178862) / 75.5 (FN554503)
Pseudomonas taiwanensisBCRC17751T / 97.9 (EU103629) / 80.5 ( HE800487) / 73.3 (HE577796)
Pseudomonas toyotomiensis HT-3T / 97.9 (AB453701) / 82.9 (AB494447) / 76.4 (HE573735)
Pseudomonas entomophilaL48T / 97.9 (AY907566) / 82.0 (AY907567) / 75.2 (AY907568)
Pseudomonas chengduensisMBRT / 97.8 (EU307111) / 82.1 (JX042516) / 78.4 (JX042517)
Pseudomonas monteilii CIP 104883T / 97.8 (AF064458) / 81.6 (FN554205) / 82.6 (FN554488)
Pseudomonas indoloxydansIPL-1T / 97.8 (DQ916277) / 81.5 (AB548144) / 76.8 (HE800496)
Pseudomonas mosselii CIP 105259T / 97.7 (AF072688) / 82.6 (FN554207) / 73.9 (FN554491)
Pseudomonas mendocinaLMG1223T / 97.7 (Z76664) / 82.6 (AJ633103) / 78.2 (AJ633567)
Pseudomonas mooreiRW10T / 97.6 (AM293566) / 81.5 (AM293560) / 71.5 (FN554489)
Pseudomonas japonicaIAM15071T / 97.6 (AB126621) / 82.9 (GQ996725) / 72.8 (HE577795)
Pseudomonas soli F-279,208T / 97.5 (HF930598) / 81.6 (HF930595) / 72.5 (HF930597)
Pseudomonas guariconensisPCAVU11T / 97.5 (HF674459) / 83.2 (HF674462) / 75.6 (HF674460)
Pseudomonas alcaligenesNBRC14159T / 97.5 (BATI01000076:175-1701) / 85.1 (AB039388) / 78.4 (AB039606)
Pseudomonas helmanticensisOHA11T / 97.4 (HG940537) / 84.3 (HG940516) / 71.4 (HG940517)
Pseudomonas fuscovaginaeICMP5940T / 97.4 (FJ483519) / 81.1 (FN554185) / 73.9 (FN554467)
Pseudomonas mohniiIpa-2T / 97.4 (AM293567) / 81.4 (AM293561) / 71.5 (FN554487)
Pseudomonas flavescensB62T / 97.3 (U01916) / 81.3 (FN554183) / 76.2 (FN554465)
Pseudomonas putidaNBRC14164T / 97.3 (AP013070) / 81.5 (AB039451) / 73.4 (D86020)
Pseudomonas aspleniiLMG2137T / 97.3 (Z76655) / 81.7 (AB039455) / 75.8 (AB039593)
FlavimonasoryzihabitansIAM1568T / 97.3 (D84004) / 83.5 (FN554210) / 75.8 (FN554494)
Pseudomonas baeticaa390T / 97.2 (FM201274) / 83.2 (HE800470) / 73.1 (FN678357)
Pseudomonas cremoricolorataIAM1541T / 97.2 (AB060137) / 80.5 (FN554181) / 74.7 (FN554462)
Pseudomonas graminis DSM 11363T / 97.2 (Y11150) / 78.9 (FN554187) / 72.0 (FN554469)
Pseudomonas benzenivorans DSM 8628T / 97.2 (FM208263) / 84.7 (HE800472) / 76.9 (HE800490)
Pseudomonas umsongensis Ps 3-10T / 97.2 (AF468450) / 81.8 (AM293564) / 71.3 (FN554516)
Pseudomonas donghuensisHYST / 97.2 (AJJP01000212: 2926-4463) / 83.0 (AJJP01000074: 11425-13842) / 72.8 (AJJP01000029: 6295-8142)
Pseudomonas vancouverensisATCC700688T / 97.1 (AJ011507) / 80.9 (AM293558) / 71.6 (FN554517)
Pseudomonas punonensisLMT03T / 97.1 (JQ344321) / 82.9 (JX435105) / 76.0 (JX435103)
Pseudomonas vranovensisCCM7279T / 97.1 (AY970951) / 82.5 (HE577791) / 71.1 (HE577793)
Pseudomonas parafulvaAJ2129T / 97.1 (AB060132) / 79.9 (FN554216) / 72.1 (FN554500)
Pseudomonas kuykendalliiH2T / 97.1 (JF749828) / 80.7 (JRPO01000013: 73511-75931) / 72.8 (JRPO01000060: 51173-53020)
Pseudomonas luteaOK2T / 97.1 (AY364537) / 78.4 (FN554198) / 71.5 (FN554480)
Pseudomonas rhizosphaeraeIH5T / 97.1 (AY152673) / 79.8 (FN554224) / 74.2 (FN554510)
HalomonaselongataATCC33173T / 87.2 (M93355) / 74.4 (FR771917) / 66.2 (FN869568)

Note:The Genbank accession numbers of 16SrRNA, gyrB and rpoD gene sequences are shown in the parentheses.

Supplementary Table S2. DNA-DNA hybridization relatedness values between strainNEAU-ST5-5T from type strains of the five most closely related species of the genus Pseudomonas.

Strain ID / NEAU-ST5-5T / P.stutzeriDSM5190T
NEAU-ST5-5T / 43 ± 1 %
P. stutzeri DSM5190T / 43 ± 1 %
P. xanthomarina DSM18231T / 40 ± 1 % / 21 ± 1 %
P. kunmingensisCGMCC1.12273T / 37 ± 1 % / 28 ± 2 %
P. alcaliphila DSM17744T / 25 ± 2 % / 25 ± 1 %
P. oleovorans subsp. lubricantisDSM21016T / 30 ± 1 % / 23 ± 2 %

Note: DNA-DNA hybridization between strains was performed in triplicate and data were presented as mean±standard deviation.

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Supplementary Fig.S1. Maximum-likelihood phylogenetic tree based on the separate16SrRNA gene sequences showing the inter-relationships of strain NEAU-ST5-5Tandtype strains of the closely related species. The16SrRNA gene sequence of HalomonaselongataATCC33173T was used as anoutgroup.Asterisks denote branches that were also recovered using the Neighbour-joining method. Bootstrap values >50 % (based on 1,000 replications) are shown at branch points. Bar, 0.02 substitutions per nucleotide position.

Supplementary Fig.S2. Maximum-likelihood phylogenetic tree based on concatenated16SrRNA, gyrB and rpoD gene sequences showing the inter-relationships of strain NEAU-ST5-5Tandtype strains of the closely related species. The concatenated16SrRNA, gyrB and rpoD gene sequence of HalomonaselongataATCC33173T was used as anoutgroup.Asterisks denote branches that were also recovered using the Neighbour-joining method. Bootstrap values >50 % (based on 1,000 replications) are shown at branch points. Bar, 0.02 substitutions per nucleotide position.The accession numbers of the sequences used in the concatenated phylogenetic tree were shown in Supplementary Table S1.

Supplementary Fig.S3. Polar lipid profile of strain NEAU-ST5-5T.

Polar lipids were separated by two-dimensional TLC and detected by spraying with molybdatophosphoric acid reagent. PE, phosphatidylethanolamine; PG, phosphatidylglycerol;DPG, diphosphatidylglycerol; UPL, unknown phospholipid; UPAL, unidentified aminophospholipid;UL, unknown lipid.

Supplementary Fig.S4. Transmission electron micrograph showing ultra-thin sections of exponentially growing cells of strain NEAU-ST-5-5T. Bar, 500 nm.

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