Supplemental Table 1. Identified T. Spiralis Proteins Recognized by Mouse Infection Sera

Supplemental Table 1. Identified T. spiralis proteins recognized by mouse infection sera at 10 dpi by shotgun LC-MS/MS

Protein name / Uniprot ID / Theor. MW(kDa) / Theor. pI / No. of peptide sequences / No. of unique peptides / Cover percent (%) / From protein bands (kDa) / Signal peptide / Transmembrane domain / Biological
process / Molecular function / Cell
Component
Large tegument protein / E5SK38 / 84.12 / 8.9 / 27 / 14 / 26.63 / 92 / No / No / proteolysis / catalytic activity; serine-type endopeptidase activity
Putative nudix hydrolase / B0F9S5 / 46.14 / 8.85 / 39 / 14 / 30.84 / 52 / No / Yes / metabolic process / hydrolase activity
Uncharacterized protein / E5SF73 / 59.49 / 8.34 / 69 / 13 / 30.23 / 52 / No / Yes
53kDa excretory/secretory antigen / Q27071 / 46.64 / 8.42 / 38 / 13 / 38.83 / 52 / Yes / No
Uncharacterized protein (Fragment) / E5SYW7 / 43.50 / 5.56 / 44 / 12 / 37.53 / 52 / No / No
Uncharacterized protein / E5SG45 / 51.05 / 5.77 / 37 / 12 / 34.15 / 52 / Yes / No
Uncharacterized protein / E5SBX5 / 56.69 / 6.79 / 29 / 12 / 28.65 / 52 / Yes / Yes
Uncharacterized protein (Fragment) / E5SBX8 / 41.44 / 9.07 / 30 / 10 / 21.66 / 52 / No / Yes
Uncharacterized protein (Fragment) / E5SZT5 / 49.06 / 9.07 / 38 / 9 / 23.27 / 52 / No / Yes
Uncharacterized protein / E5S3K0 / 48.85 / 5.15 / 20 / 9 / 33.18 / 52 / No / Yes
Putative ISDet3, transposase OrfA / E5S9D6 / 49.89 / 4.73 / 8 / 6 / 17.77 / 52 / Yes / No
Uncharacterized protein (Fragment) / E5T014 / 29.98 / 9.64 / 14 / 5 / 18.82 / 52 / No / Yes
Uncharacterized protein / E5SVY5 / 58.25 / 8.38 / 8 / 4 / 11.15 / 52 / Yes / Yes / metabolic process / hydrolase activity
Serine protease / B7SIW4 / 35.21 / 5.97 / 4 / 2 / 8.95 / 52 / Yes / No / proteolysis / catalytic activity; serine-type endopeptidase activity; peptidase activity
Putative sodium/potassium-transporting ATPase subunit beta-1 / E5SK40 / 91.35 / 5.38 / 2 / 2 / 3.21 / 52 / No / Yes / proteolysis; potassium ion transport; sodium ion transport / catalytic activity; serine-type endopeptidase activity / sodium: potassium-exchanging ATPase complex
Multi cystatin-like domain protein / A1BQX7 / 45.97 / 5.35 / 30 / 13 / 37.93 / 45 / Yes / No
Antigen targeted by protective antibodies / Q27074 / 31.26 / 4.76 / 7 / 2 / 9.47 / 45 / Yes / No / proteolysis / catalytic activity; serine-type endopeptidase activity
Serine protease / Q9BJM1 / 71.62 / 8.83 / 50 / 18 / 36.43 / 35 / Yes / No / proteolysis / catalytic activity; serine-type endopeptidase activity; peptidase activity; serine-type peptidase activity; hydrolase activity
Putative trypsin / E5RYV9 / 71.61 / 8.83 / 50 / 17 / 32.08 / 35 / Yes / No / proteolysis / catalytic activity; serine-type endopeptidase activity; peptidase activity; serine-type peptidase activity; hydrolase activity
Deoxyribonuclease II family protein / E5RZG8 / 38.07 / 7.57 / 18 / 9 / 35.73 / 35 / Yes / No / DNA metabolic process / deoxyribonuclease II activity
Putative uncharacterized protein / Q962V2 / 30.06 / 8.66 / 17 / 8 / 36.96 / 35 / No / No / DNA metabolic process / deoxyribonuclease II activity
Adult-specific DNase II-3 / Q32R73 / 38.45 / 7.58 / 17 / 8 / 29.23 / 35 / Yes / No / DNA metabolic process / deoxyribonuclease II activity
Adult-specific DNase II-6 (Fragment) / Q32R70 / 33.60 / 8.65 / 15 / 8 / 30.1 / 35 / No / No / DNA metabolic process / deoxyribonuclease II activity
Deoxyribonuclease II family protein / E5SGF7 / 38.00 / 6.99 / 15 / 7 / 25.94 / 35 / Yes / No / DNA metabolic process / deoxyribonuclease II activity
Adult-specific DNase II-10 / Q32R66 / 38.25 / 6.52 / 12 / 7 / 29.31 / 35 / Yes / No / DNA metabolic process / deoxyribonuclease II activity
Adult-specific DNase II-9 / Q32R67 / 38.18 / 8.06 / 10 / 7 / 25.29 / 35 / Yes / No / DNA metabolic process / deoxyribonuclease II activity
Adult-specific DNase II-8 / Q32R68 / 38.18 / 8.07 / 13 / 6 / 22.13 / 35 / Yes / No / DNA metabolic process / deoxyribonuclease II activity
DNase II-ll-like protein (Fragment) / A0MTB2 / 24.78 / 8.7 / 11 / 6 / 33.62 / 35 / No / No / DNA metabolic process / deoxyribonuclease II activity
Adult-specific DNase II-7 / Q32R69 / 38.22 / 7.56 / 11 / 6 / 20.98 / 35 / Yes / No / DNA metabolic process / deoxyribonuclease II activity
Deoxyribonuclease II family protein / E5SGG7 / 38.23 / 6.94 / 11 / 6 / 25.86 / 35 / Yes / No / DNA metabolic process / deoxyribonuclease II activity
Adult-specific DNase II-11 (Fragment) / Q32R65 / 34.48 / 8.75 / 9 / 6 / 22.47 / 35 / No / No / DNA metabolic process / deoxyribonuclease II activity
Putative serine protease / B0F9T9 / 47.55 / 8.73 / 8 / 4 / 12.12 / 35 / Yes / No / proteolysis / catalytic activity; serine-type endopeptidase activity; peptidase activity; serine-type peptidase activity; hydrolase activity
Uncharacterized protein / E5SAA1 / 109.28 / 5.76 / 5 / 4 / 5.25 / 35 / No / No / regulation of cyclin-dependent protein serine/threonine kinase activity; regulation of transcription, DNA-templated; proteolysis / catalytic activity; serine-type endopeptidase activity; protein kinase binding
Multi cystatin-like domain protein / A1BQX7 / 45.97 / 5.35 / 4 / 4 / 13.79 / 35 / Yes / No
Uncharacterized protein / E5T2P5 / 28.14 / 5.05 / 5 / 3 / 14.23 / 35 / Yes / Yes
Putative uncharacterized protein ad48h-Ts3 (Fragment) / F0V432 / 33.85 / 8.81 / 7 / 2 / 9.06 / 35 / No / No
Antigen targeted by protective antibodies / Q27074 / 31.26 / 4.76 / 6 / 2 / 9.47 / 35 / Yes / No / proteolysis / catalytic activity; serine-type endopeptidase activity
AP-1 complex subunit beta-1 / E5S0E7 / 46.65 / 8.7 / 4 / 2 / 7.39 / 35 / Yes / No
MAP kinase-activating death domain protein / E5RYD6 / 31.26 / 4.36 / 4 / 2 / 9.57 / 35 / Yes / No / phosphorylation / catalytic activity; kinase activity
Uncharacterized protein / E5SH24 / 57.43 / 8.96 / 3 / 2 / 4.77 / 35 / No / No
Uncharacterized protein / E5RZ94 / 36.88 / 8.84 / 3 / 2 / 6.44 / 35 / Yes / No
Putative venom allergen 5 / E5SVR8 / 50.20 / 9.2 / 3 / 2 / 7.49 / 35 / Yes / No
Putative serine protease / B0F9T9 / 47.55 / 8.73 / 20 / 8 / 26.34 / 32 / Yes / No / proteolysis / catalytic activity; serine-type endopeptidase activity; peptidase activity; serine-type peptidase activity; hydrolase activity
Putative venom allergen 5 / E5SVR8 / 50.20 / 9.2 / 13 / 7 / 23.35 / 32 / Yes / No
Serine protease / Q9BJM1 / 71.62 / 8.83 / 11 / 7 / 14.69 / 32 / Yes / No / proteolysis / catalytic activity; serine-type endopeptidase activity; peptidase activity; serine-type peptidase activity; hydrolase activity
Putative trypsin / E5SE12 / 49.89 / 8.94 / 17 / 6 / 16.93 / 32 / Yes / No / proteolysis / catalytic activity; serine-type endopeptidase activity; peptidase activity; serine-type peptidase activity; hydrolase activity
Multi cystatin-like domain protein / A1BQX7 / 45.97 / 5.35 / 12 / 6 / 22.17 / 32 / Yes / No
Putative serine protease / B0F9T6 / 47.36 / 8.64 / 13 / 5 / 13.99 / 32 / Yes / No / proteolysis / catalytic activity; serine-type endopeptidase activity; peptidase activity; serine-type peptidase activity; hydrolase activity
Uncharacterized protein / E5SY67 / 33.44 / 8.09 / 7 / 5 / 21.14 / 32 / Yes / No
Uncharacterized protein / E5T2P5 / 28.14 / 5.05 / 4 / 3 / 14.63 / 32 / Yes / Yes
Secreted from muscle stage larvae 1 / B6SEV2 / 31.81 / 7.43 / 4 / 3 / 12.19 / 32 / Yes / Yes
AP-1 complex subunit beta-1 / E5S0E7 / 46.65 / 8.7 / 5 / 2 / 7.39 / 32 / Yes / No
43 kD secreted glycoprotein / Q27073 / 37.70 / 5.95 / 5 / 2 / 8.14 / 32 / Yes / No / DNA metabolic process / deoxyribonuclease II activity
Uncharacterized protein / E5SAQ2 / 34.91 / 8.82 / 3 / 2 / 7.38 / 32 / Yes / No
DNase II-ll-like protein (Fragment) / A0MTB2 / 24.78 / 8.7 / 2 / 2 / 10.92 / 32 / No / No / DNA metabolic process / deoxyribonuclease II activity
MAP kinase-activating death domain protein / E5RYD6 / 31.26 / 4.36 / 2 / 2 / 11.35 / 32 / Yes / No / phosphorylation / catalytic activity; kinase activity
Uncharacterized protein (Fragment) / E5SVT5 / 28.07 / 4.85 / 2 / 2 / 8.63 / 32 / No / No / metabolic process / catalytic activity
Cystatin-like protein / B0FJW6 / 27.56 / 8.02 / 25 / 8 / 43.04 / 29 / Yes / No
Putative uncharacterized protein ad48h-Ts3 (Fragment) / F0V432 / 33.85 / 8.81 / 11 / 7 / 27.18 / 29 / No / No
Uncharacterized protein / E5SXP9 / 58.91 / 9.17 / 9 / 6 / 12.43 / 29 / No / No / regulation of transcription, DNA-templated / nucleus
Putative trypsin / E5S7R2 / 62.02 / 9.21 / 12 / 5 / 12.52 / 29 / No / No / proteolysis / catalytic activity; serine-type endopeptidase activity
Secreted from muscle stage larvae 1 / B6SEV2 / 31.81 / 7.43 / 7 / 5 / 22.22 / 29 / Yes / Yes
Peptidase, S1A subfamily / E5S2E7 / 28.66 / 8.32 / 4 / 3 / 13.13 / 29 / No / No / proteolysis / catalytic activity; serine-type endopeptidase activity
Uncharacterized protein / E5SEX2 / 26.51 / 8.79 / 4 / 2 / 7.17 / 29 / No / No
Uncharacterized protein / E5S741 / 39.27 / 4.97 / 3 / 2 / 3.82 / 29 / Yes / Yes
Uncharacterized protein / E5SVT0 / 16.32 / 4.8 / 2 / 2 / 19.86 / 29 / No / No