Supplementary material. S-Table 3 Specificity tests using SYBR Green fluorescence and real time qPCR experiments for the evaluation of the 15 set of primers against these species and with S. rarum (Srar), S. riobrave (Srio), Steinernema sp. glaseri-group (Sx) and H. indica (Hi). Data from the averaged melting temperature (Tm), if registered, is presented in °C. The Tm for the right combination of primers and target species is highlighted in bold; n.a., amplifications that gave not appreciable melting curves

Primers sets / Species of nematodes used for amplifications
Sf / Sca / Sg / Sx / Sbic / Srio / Srar / Saff / Swei / Skr / Ssilv / Sint / Sint-g / Spoin / Hm / Hb / Hi / Hz / Acrob
S. feltiae (Sf) / 78.4 / – / – / – / n.a. / – / – / – / 79.8 / – / – / – / – / – / – / – / – / – / –
S. carpocapsae (Sca) / – / 81.0 / – / – / – / – / – / – / – / – / – / – / – / – / – / – / – / – / –
S. glaseri (Sg) / – / – / 85.0 / – / – / – / – / – / – / – / – / – / – / – / – / – / – / – / –
S. bicornutum (Sbic) / – / – / – / – / 79.0 / – / – / – / – / – / – / – / – / – / – / – / – / – / –
S. affine (Saff) / – / – / – / – / – / – / – / 79.0 / – / – / – / – / – / – / – / – / – / – / –
S. weiseri (Swei) / – / – / – / – / – / – / – / – / 80.4 / – / – / – / – / – / – / – / – / – / –
S. kraussei (Skr) / 81.2 / – / – / – / – / – / – / – / 80.3 / 80.5 / 80.7 / – / – / – / – / – / – / – / –
S. silvaticum (Ssilv) / – / – / – / – / – / – / – / n.a. / – / 80.5 / 80.7 / – / – / – / – / – / – / – / –
S. intermedium (Sint) / – / – / – / – / – / – / – / – / – / – / 77.0 / 76.1 / – / – / – / – / – / – / –
Steinernema sp. intermedium-group (Sint-g) / – / – / – / – / n.a. / – / – / 78.1 / – / – / – / n.a. / 78.1 / 78.0 / – / – / – / – / –
S. poinari (Spoin) / – / – / – / – / – / – / – / 76.3 / n.a. / – / – / – / n.a. / 76.0 / – / – / – / – / –
H. megidis (Hm) / – / – / – / – / – / – / – / – / – / – / – / – / – / – / 82.9 / – / – / – / –
H. bacteriophora (Hb) / – / – / – / – / – / – / – / – / – / – / – / – / – / – / – / 80.5 / – / – / –
H. zealandica (Hz) / – / – / – / – / – / – / – / – / – / – / – / – / – / – / – / – / 77.8 / 76.8 / –
Acrobeloides-group (Acrob) / – / – / – / – / – / – / – / – / – / – / – / – / – / – / – / – / – / – / 83.5