UOW Laboratory Safety 2000

UNIVERSITY OF WESTMINSTER

School of Biosciences

Control of Substances Hazardous to Health (COSHH)

***** APPENDIX 4 - CHEMICAL SAFETY *****

Sheet...... 1... of...1......

Title of Experiment Room/Laboratory Site

1. / 3BCM505 Proteins and Enzymes PROTEIN PURIFICATION / 7th Floor Teaching Laboratory / NCS

Brief description of work

Students determine the specific activity of wheat germ acid phosphatase by carrying out a timed enzyme assay and protein concentration determination using Coomassie blue (Bradford) reagent.

Note that a COSHH assessment for class activity may not be of a sufficiently high level to cover technician preparation; double assessment usually required. Note also that it is RISK that is to be assessed, not HAZARD.

2.LIST OF SUBSTANCES

NAME OF SUBSTANCE
(CODE) / PHYSICAL
DESCRIPTION / MAX.
QUANTITY
EXPOSED TO / DATA AVAILABLE
(YES/NO) / KNOWN HAZARDS
(See Note;
use numbers)
Sodium Hydroxide (0.1 M) / Colourless liquid / ml / Y / 5 (Harmful)
6 (Corrosive)
7 (Irritant)
13 (Ingestion)
14 (Skin Absorption)
15 (Skin and Eyes)
16 (Ingestion)
Sodium Acetate (0.05 M; pH 5.0) / White powder or colourless liquid / mg / Y / 7 (Irritant)
14 (Skin Absorption)
15 (Skin and Eyes)
p-nitrophenyl phosphate (0.01M) / White powder or colourless liquid / mg / Y / 7 (Irritant)
14 (Skin Absorption)
15 (Skin and Eyes)
Acid phosphatase (from wheat germ) / White powder or cloudy liquid / mg / Y / 20 (Sensitization)*
* may cause allergic reaction upon prolonged or repeated exposure
Bovine Serum Albumin / White powder or colourless liquid / mg / N
Coomassie Blue (Bradford Reagent) / Blue coloured liquid / ml / Y / 4 (Toxic)
5 (Harmful)
6 (Corrosive)
7 (Irritant)
13 (Ingestion)
14 (Skin Absorption)
15 (Skin and eyes)

NOTE: 1) EXPLOSIVE 2) OXIDIZING 3) FLAMMABLE 4) TOXIC 5) HARMFUL 6) CORROSIVE

7) IRRITANT 8) CARCINOGEN 9) MUTAGEN 10) TERATOGEN 11) DUST 12) INHALATION

13) INGESTION 14) SKIN ABSORPTION 15) SKIN OR EYE 16) INJECTION 17) MAXIMUM EXPOSURE LIMIT

18) OCCUPATIONAL EXPOSURE STANDARD 19) RADIATION 20) OTHER (specify) Ref Note 17, 18: see EH40 (current edition).

3.PERSONNEL INVOLVED WITH SUBSTANCES (continue on a separate sheet if necessary)

NAME/CLASS / INITS / STATUS / INVOLVEMENT - DATE OF USE or
DAILY/WEEKLY/MONTHLY/OCCASIONALLY
3BCM505 Class / Once per year
Curley / P / Lecturer / Occasionally
Bucke / C / Lecturer / Occasionally
Perry / D / Lecturer / Occasionally
Thompson / S / Lecturer / Occasionally

4.OTHER GROUPS/PERSONS WHO MAY HAVE ACCESS TO THE SUBSTANCES

(e.g. students, cleaners, maintenance staff, contractors, visitors, storekeepers, etc.):

Reagents are available to students (under supervision) and Technical/Stores staff only.

State control measures (to prevent inappropriate persons/groups having access to the substances):

Reagents are only made available to students on the day of laboratory session. Once the practical session is completed reagents are either disposed of or stored away by the technical staff.

EMERGENCY PROCEDURES (as per Hazard Data Sheet).

if any of the substances or procedures identified above is likely to pose a special hazard in an emergency, then identify below action to be taken.

CODE / SPILLAGE/UNCONTROLLEDRELEASE / FIRE
None / None
If personnel are affected (fumes, contamination, etc.) treatment to be adopted: SPECIFY PER SUBSTANCE
(CODE AS PER HAZARD DATA SHEET)
Sodium Acetate Wash skin/eye wash
p-nitrophenyl phosphate Wash skin/eye wash
Acid phosphatase Wash skin/eye wash
Bovine Serum Albumin Wash skin/eye wash
Coomassie Blue (Bradford Reagent) Wash skin/eye wash*
NaOH Wash skin/eye wash**
* In case of contact with eyes, flush with copious amounts of water for at least 15 min. Ensure adequate flushing by separating eyelids with fingers. CONSULT A PHYSICIAN AS SOON AS POSSIBLE. If swallowed, wash the mouth (if person is conscious) with copious amounts of water. Do NOT induce vomiting. CALL A PHYSICIAN.
** In case of contact with eyes, flush with copious amounts of water for at least 15 min. Ensure adequate flushing by separating eyelids with fingers. CONSULT A PHYSICIAN AS SOON AS POSSIBLE.
(NB. Antidotes and special treatment may be obtained through...... N/A...... )

UOW Laboratory Safety 2000

6.CONTROL MEASURES TO BE ADOPTED (NB. Consider the following points, the appropriate Biosciences Safety Code plus any other necessary measures. SPECIFY PER SUBSTANCE AS NECESSARY FOR EACH OF THE ITEMS BELOW.)

1. Limiting exposure time of personnel - state maximum time

2. Partial enclosure with local exhaust ventilation

3. Local exhaust ventilation

4. Sufficient general ventilation

5. Personal protective equipment (goggles/gloves/clothing/mask/breathing apparatus/safety screen, etc.) SPECIFY

* 6. Safe disposal/storage of hazardous substances

* 7. Regular cleaning of the workplace

* 8. Adequate washing facilities* = MANDATORY

STORAGE - SAFETY CONSIDERATIONS
Commassie Reagent stock solutions should be kept in fume hood when not is use. /

HANDLING PRECAUTIONS

Disposable rubber gloves and fume hood should be used when dispensing large volumes (> 10 ml) of Coomassie Reagent.
DISPOSAL PROCEDURES DURING AND AT END OF EXPERIMENT
Unused reagents can be safely disposed of down the drain. Wash sink with copious amounts of water.

7.REVIEW AND MONITORING OF CONTROL MEASURES

(Required checks, and their frequency, on the adequacy and maintenance of the control measures during the course of the experiment)

(NB. Biosciences Safety Code in respect of health surveillance needs. This form is not adequate if health surveillance is required.)

8.OTHER RELEVANT INFORMATION (continue on a separate sheet if necessary)

(e.g. restriction of usage; transportation risks; labelling requirements; assessment review date, etc.)

None.

9.I certify that, to the best of my knowledge, the above precautions will adequately control the risks from the hazardous substances listed. I have brought the assessment to the notice of the relevant technicians; academic staff; students; researchers (delete as necessary).

Name of Assessor:Dr Paul CurleySigned:

Status of Assessor:Senior LecturerDate: 27th September, 2003

UOW Laboratory Safety 2000