MUESTRA 2
Protein View
Principio del formulario
Match to: gi|30794280 Score: 342 Expect: 3.5e-28
albumin [Bos taurus]
Nominal mass (Mr): 71274; Calculated pI value: 5.82
NCBI BLAST search of gi|30794280 against nr
Unformatted sequence string for pasting into other applications
Taxonomy: Bos taurus
Links to retrieve other entries containing this sequence from NCBI Entrez:
gi|2190337 from Bos taurus
gi|3336842 from Bos taurus
gi|23307791 from Bos taurus
gi|148744077 from Bos taurus
Fixed modifications: Carbamidomethyl (C)
Variable modifications: Oxidation (M)
Cleavage by Trypsin: cuts C-term side of KR unless next residue is P
Number of mass values searched: 81
Number of mass values matched: 39
Sequence Coverage: 62%
Matched peptides shown in Bold Red
1 MKWVTFISLL LLFSSAYSRG VFRRDTHKSE IAHRFKDLGE EHFKGLVLIA
51 FSQYLQQCPF DEHVKLVNEL TEFAKTCVAD ESHAGCEKSL HTLFGDELCK
101 VASLRETYGD MADCCEKQEP ERNECFLSHK DDSPDLPKLK PDPNTLCDEF
151 KADEKKFWGK YLYEIARRHP YFYAPELLYY ANKYNGVFQE CCQAEDKGAC
201 LLPKIETMRE KVLTSSARQR LRCASIQKFG ERALKAWSVA RLSQKFPKAE
251 FVEVTKLVTD LTKVHKECCH GDLLECADDR ADLAKYICDN QDTISSKLKE
301 CCDKPLLEKS HCIAEVEKDA IPENLPPLTA DFAEDKDVCK NYQEAKDAFL
351 GSFLYEYSRR HPEYAVSVLL RLAKEYEATL EECCAKDDPH ACYSTVFDKL
401 KHLVDEPQNL IKQNCDQFEK LGEYGFQNAL IVRYTRKVPQ VSTPTLVEVS
451 RSLGKVGTRC CTKPESERMP CTEDYLSLIL NRLCVLHEKT PVSEKVTKCC
501 TESLVNRRPC FSALTPDETY VPKAFDEKLF TFHADICTLP DTEKQIKKQT
551 ALVELLKHKP KATEEQLKTV MENFVAFVDK CCAADDKEAC FAVEGPKLVV
601 STQTALA
Residue Number Increasing Mass Decreasing Mass
Start - End Observed Mr(expt) Mr(calc) ppm Miss Sequence
35 - 44 1249.6610 1248.6537 1248.6139 32 1 R.FKDLGEEHFK.G
37 - 44 974.5135 973.5062 973.4505 57 0 K.DLGEEHFK.G
66 - 75 1163.6433 1162.6361 1162.6234 11 0 K.LVNELTEFAK.T
89 - 100 1419.7192 1418.7119 1418.6864 18 0 K.SLHTLFGDELCK.V
123 - 130 1034.5183 1033.5110 1033.4651 44 0 R.NECFLSHK.D
139 - 151 1576.8161 1575.8089 1575.7603 31 0 K.LKPDPNTLCDEFK.A
161 - 167 927.5251 926.5178 926.4861 34 0 K.YLYEIAR.R
169 - 183 1889.0163 1888.0091 1887.9195 47 0 R.HPYFYAPELLYYANK.Y
184 - 197 1747.7499 1746.7426 1746.6978 26 0 K.YNGVFQECCQAEDK.G
249 - 256 922.5050 921.4978 921.4807 18 0 K.AEFVEVTK.L
267 - 280 1749.7518 1748.7446 1748.6553 51 0 K.ECCHGDLLECADDR.A
286 - 297 1443.7159 1442.7086 1442.6347 51 0 K.YICDNQDTISSK.L
298 - 309 1532.8386 1531.8313 1531.7738 38 1 K.LKECCDKPLLEK.S
300 - 309 1291.6343 1290.6270 1290.5948 25 0 K.ECCDKPLLEK.S
310 - 318 1072.5493 1071.5420 1071.5019 37 0 K.SHCIAEVEK.D
319 - 336 1956.0732 1955.0660 1954.9524 58 0 K.DAIPENLPPLTADFAEDK.D
319 - 340 2458.3545 2457.3472 2457.1733 71 1 K.DAIPENLPPLTADFAEDKDVCK.N
347 - 359 1567.8129 1566.8056 1566.7354 45 0 K.DAFLGSFLYEYSR.R
360 - 371 1439.8657 1438.8584 1438.8045 38 1 R.RHPEYAVSVLLR.L
361 - 371 1283.7525 1282.7452 1282.7034 33 0 R.HPEYAVSVLLR.L
375 - 386 1502.6619 1501.6546 1501.6065 32 0 K.EYEATLEECCAK.D
387 - 399 1554.7489 1553.7416 1553.6457 62 0 K.DDPHACYSTVFDK.L
402 - 412 1305.7490 1304.7418 1304.7088 25 0 K.HLVDEPQNLIK.Q
413 - 420 1068.4783 1067.4710 1067.4342 35 0 K.QNCDQFEK.L
421 - 433 1479.8556 1478.8483 1478.7881 41 0 K.LGEYGFQNALIVR.Y
437 - 451 1640.0023 1638.9950 1638.9305 39 1 R.KVPQVSTPTLVEVSR.S
438 - 451 1511.9064 1510.8991 1510.8355 42 0 K.VPQVSTPTLVEVSR.S
469 - 482 1740.8863 1739.8791 1739.8222 33 0 R.MPCTEDYLSLILNR.L Oxidation (M)
483 - 489 898.4841 897.4768 897.4742 3 0 R.LCVLHEK.T
499 - 507 1138.5515 1137.5442 1137.4907 47 0 K.CCTESLVNR.R
508 - 523 1881.0158 1880.0085 1879.9138 50 0 R.RPCFSALTPDETYVPK.A
529 - 544 1907.9864 1906.9792 1906.9135 34 0 K.LFTFHADICTLPDTEK.Q
548 - 557 1142.7376 1141.7303 1141.7070 20 1 K.KQTALVELLK.H
562 - 568 818.4329 817.4257 817.4181 9 0 K.ATEEQLK.T
569 - 580 1399.7062 1398.6989 1398.6853 10 0 K.TVMENFVAFVDK.C
569 - 580 1415.6970 1414.6897 1414.6803 7 0 K.TVMENFVAFVDK.C Oxidation (M)
581 - 597 1927.9180 1926.9107 1926.7910 62 1 K.CCAADDKEACFAVEGPK.L
588 - 597 1107.5506 1106.5433 1106.5066 33 0 K.EACFAVEGPK.L
598 - 607 1002.5166 1001.5093 1001.5757 -66 0 K.LVVSTQTALA.-
No match to: 807.3558, 825.4265, 827.4571, 830.4364, 842.5445, 846.5916, 856.5511, 870.5788, 880.4964, 912.4357, 955.4827, 960.4442, 965.4790, 997.6232, 1045.6326, 1051.4451, 1082.5225, 1089.4370, 1121.5279, 1126.5933, 1149.5631, 1179.6477, 1201.6195, 1235.6192, 1265.6378, 1277.7109, 1343.7217, 1467.7599, 1491.7684, 1504.7381, 1542.7502, 1766.8062, 1778.9002, 1802.9917, 1910.8798, 1940.9990, 2211.2386, 2225.2913, 2239.2193, 2249.1748, 2283.3197, 2809.5011
Final del formulario
LOCUS NP_851335 607 aa linear MAM 07-OCT-2007
DEFINITION albumin [Bos taurus].
ACCESSION NP_851335
VERSION NP_851335.1 GI:30794280
DBSOURCE REFSEQ: accession NM_180992.2
KEYWORDS .
SOURCE Bos taurus (cattle)
ORGANISM Bos taurus
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
Pecora; Bovidae; Bovinae; Bos.
REFERENCE 1 (residues 1 to 607)
AUTHORS Vicente-Serrano,J., Caballero,M.L., Rodriguez-Perez,R.,
Carretero,P., Perez,R., Blanco,J.G., Juste,S. and Moneo,I.
TITLE Sensitization to serum albumins in children allergic to cow's milk
and epithelia
JOURNAL Pediatr Allergy Immunol 18 (6), 503-507 (2007)
PUBMED 17680908
REMARK GeneRIF: Bovine serum albumin is common allergen responsible for
cow's milk allergy. Cross reactivity with serum albumins in
meat/epithelial cells of other mammals results.
REFERENCE 2 (residues 1 to 607)
AUTHORS Schlesinger,J.B., van Harmelen,V., Alberti-Huber,C.E. and Hauner,H.
TITLE Albumin inhibits adipogenesis and stimulates cytokine release from
human adipocytes
JOURNAL Am. J. Physiol., Cell Physiol. 291 (1), C27-C33 (2006)
PUBMED 16452161
REMARK GeneRIF: Human preadipocytes and freshly isolated adipocytes
incubated with bovine serum albumin (BSA) in vitro secrete
significantly higher amounts of cytokines IL-6, -8, and -10, and
TNF-alpha compared with cells incubated without BSA.
REFERENCE 3 (residues 1 to 607)
AUTHORS Togashi,D.M. and Ryder,A.G.
TITLE Time-resolved fluorescence studies on bovine serum albumin
denaturation process
JOURNAL J Fluoresc 16 (2), 153-160 (2006)
PUBMED 16382334
REMARK GeneRIF: The results from the models show that there are at least
two different binding sites located in the BSA protein with
different water accessibility
REFERENCE 4 (residues 1 to 607)
AUTHORS Duncan,T., Fariss,R.N. and Wiggert,B.
TITLE Confocal immunolocalization of bovine serum albumin, serum
retinol-binding protein, and interphotoreceptor retinoid-binding
protein in bovine retina
JOURNAL (er) Mol. Vis. 12, 1632-1639 (2006)
PUBMED 17200663
REMARK GeneRIF: Serum albumin and serum retinol-binding protein(sRBP) are
not components of bovine interphotoreceptor matrix(IPM). Serum
albumin and sRBP can not participate in binding and transport of
visual cycle retinoids in IPM of bovine retina.
REFERENCE 5 (residues 1 to 607)
AUTHORS Wolf,G., Schroeder,R., Ziyadeh,F.N. and Stahl,R.A.
TITLE Albumin up-regulates the type II transforming growth factor-beta
receptor in cultured proximal tubular cells
JOURNAL Kidney Int. 66 (5), 1849-1858 (2004)
PUBMED 15496155
REMARK GeneRIF: Albumin up-regulates ligand-binding TGF-beta receptors on
cultured proximal tubular cells. Albumin-induced activation of
local Ang II production appears to be responsible for this effect.
REFERENCE 6 (residues 1 to 607)
AUTHORS Hirayama,K., Akashi,S., Furuya,M. and Fukuhara,K.
TITLE Rapid confirmation and revision of the primary structure of bovine
serum albumin by ESIMS and Frit-FAB LC/MS
JOURNAL Biochem. Biophys. Res. Commun. 173 (2), 639-646 (1990)
PUBMED 2260975
REFERENCE 7 (residues 1 to 607)
AUTHORS Strawich,E. and Glimcher,M.J.
TITLE Tooth 'enamelins' identified mainly as serum proteins. Major
'enamelin' is albumin
JOURNAL Eur. J. Biochem. 191 (1), 47-56 (1990)
PUBMED 2379503
REFERENCE 8 (residues 1 to 607)
AUTHORS Carraway,R.E., Mitra,S.P. and Cochrane,D.E.
TITLE Structure of a biologically active neurotensin-related peptide
obtained from pepsin-treated albumin(s)
JOURNAL J. Biol. Chem. 262 (13), 5968-5973 (1987)
PUBMED 2437111
REFERENCE 9 (residues 1 to 607)
AUTHORS McGillivray,R.T., Chung,D.W. and Davie,E.W.
TITLE Biosynthesis of bovine plasma proteins in a cell-free system.
Amino-terminal sequence of preproalbumin
JOURNAL Eur. J. Biochem. 98 (2), 477-485 (1979)
PUBMED 488109
REFERENCE 10 (residues 1 to 607)
AUTHORS Patterson,J.E. and Geller,D.M.
TITLE Bovine microsomal albumin: amino terminal sequence of bovine
proalbumin
JOURNAL Biochem. Biophys. Res. Commun. 74 (3), 1220-1226 (1977)
PUBMED 843354
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence was derived from X58989.1.
Publication Note: This RefSeq record includes a subset of the
publications that are available for this gene. Please see the
Entrez Gene record to access additional publications.
FEATURES Location/Qualifiers
source 1..607
/organism="Bos taurus"
/db_xref="taxon:9913"
/chromosome="6"
/map="6"
Protein 1..607
/product="albumin"
/calculated_mol_wt=69193
Region 29..208
/region_name="ALBUMIN"
/note="Albumin domain, contains five or six internal
disulphide bonds; albuminoid superfamily includes
alpha-fetoprotein which binds various cations, fatty acids
and bilirubin; vitamin D-binding protein which binds to
vitamin D, its metabolites, and fatty acids; cd00015"
/db_xref="CDD:28899"
Site order(51,55,70,91,95,98,123,126..127)
/site_type="binding"
/db_xref="CDD:28899"
Site order(158,161..162,165,181,184)
/site_type="binding"
/db_xref="CDD:28899"
Region 220..400
/region_name="ALBUMIN"
/note="Albumin domain, contains five or six internal
disulphide bonds; albuminoid superfamily includes
alpha-fetoprotein which binds various cations, fatty acids
and bilirubin; vitamin D-binding protein which binds to
vitamin D, its metabolites, and fatty acids; cd00015"
/db_xref="CDD:28899"
Site order(242,246,261,280,284,287,310,313..314)
/site_type="binding"
/db_xref="CDD:28899"
Site order(350,353..354,357,373,376)
/site_type="binding"
/db_xref="CDD:28899"
Region 412..598
/region_name="ALBUMIN"
/note="Albumin domain, contains five or six internal
disulphide bonds; albuminoid superfamily includes
alpha-fetoprotein which binds various cations, fatty acids
and bilirubin; vitamin D-binding protein which binds to
vitamin D, its metabolites, and fatty acids; cd00015"
/db_xref="CDD:28899"
Site order(434,438,453,476,480,483,508,511..512)
/site_type="binding"
/db_xref="CDD:28899"
Site order(548,551..552,555,571,574)
/site_type="binding"
/db_xref="CDD:28899"
CDS 1..607
/gene="ALB"
/coded_by="NM_180992.2:33..1856"
/db_xref="GeneID:280717"