The number of biological and technical replicates for each experiment. A biological replicate refers to 1 dissection and plating procedure. A technical replicate refers to 1 well of a 96-well plate from within a biological replicate.
Experiment / Figure / Isolations (n) / Technical replicates / Data pointsper condition / conditions / total
Cell count / 1b / 2 / 8 / 16 / 6 / 96
Calcium activity over time / 1f / 8 / 3 / 24 / 5 / 120
NO & SD over time / 2b / 2 / 6 / 12 / 5 / 60
Dendritic spines over time / 2d / 2 / 750 µm; 5 images / 1500 µm; 10 images / 5 / 7500 µm; 50 images
Astrocytes: calcium imaging / 3a / 2 / 3 / 6 / 6 x 3 / 108
Astrocytes: count / 3b / 2 / 2 / 4 / 6 x 2 / 48
Astrocytes: NO & SD / 3c / 2 / 6 / 12 / 6 / 72
Astrocytes: spines / 3c / 2 / 1250 µm; 8 images / 2500 µm; 16 images / 6 / 15000 µm; 96 images
Anti-NGF: calcium imaging / 4a / 2 / 3 / 6 / 5 x 3 / 90
Anti-NGF: NO & SD / 4b / 2 / 4 / 8 / 5 / 40
Anti-NGF: spines / 4b / 2 / 750 µm; 5 images / 1500 µm; 10 images / 5 / 7500 µm; 50 images
Forskolin / 5c / 3 / 2 / 6 / 5 / 30
MK-801 / 5e / 3 / 2 / 6 / 4 / 24
NMDA-R qPCR / 6a / 3 / 3 / 9 / 5 x 3 / 135
NMDA-R: recording medium / 6b, 6f / 16 / 5 / 80 / 2 / 160
NMDA-R: ket/KA/QA / 6c-e, 6g-i / 3 / 1-2 / 5 / 4 x 2 x 3 / 120
NMDA-R: successive addition / 6j-m / 3 / 2-3 / 7 / 4 / 28
NO & SD: Neurite outgrowth & synapse density