Supplement table 2. Oligonucleotide primers used for the sequencing of the M RNA segment of SATV, DOUV and SHAV

Primer / Sequence (5’-3’) / Position*** / Purpose
For SATV
SATVM3R6* / CTATTGAAGTGACATGTGGAT / 502-529 / sequencing
SATVM 3F6 / CAAGATTTGTACAAAATGATTC / 619-640 / sequencing
SATVM 3R5 / ATACTTACAGTTTTATATATTCTTGTG / 1,106-1,132 / sequencing
SATVM 3F5 / CGTCTTTCATGTATCATACCG / 1,203-1,223 / sequencing
SATVM3R4 / GATCATGCTAGGGAGATGTC / 1819-1838 / sequencing
SATVM 3F4 / CACATCTGCATAGACTCTAAGC / 1781-1802 / sequencing
SATVM 3F3* / TCCAGTGCTATATGGTGATTC / 2404-2424 / sequencing
SATVM 3R3 / GTTTCAGCTAAATCTCATTCAAG / 2518-2540 / sequencing
SATVM 3R2 / ATCAAGACGGTTGGATCACT / 3,050-3,069 / sequencing
SATVM 3F2* / TGAACCATATAAGCAACCAT / 3,131-3,150 / sequencing
SATVM3R1* / GAAATTAGGTGTGGTGGATGCT / 3,694-3,715 / sequencing
SATVM3F1* / TAAGTGAGCAGGCCATTCCT / 3729-3,748 / sequencing
For DOUV
DOUVM 3F6 / CAAGATTTGTACAAAATGACTC / 619-640 / sequencing
DOUVM 3R5 / CTTATGGTTTTATATATTCTTGTGC / 1,109-1,133 / sequencing
DOUVM 3F5 / ACGTCTTTCATGTATCATTCC / 1,204-1,224 / sequencing
DOUVM 3F4 / GCTGCATAGATTCTAAACACC / 1,778-1,798 / sequencing
DOUVM 3R4 / GATCATGCCAGGGAGATGTC / 1,819-1,838 / sequencing
For SHAV
SHAVM 3R6 / GACATTTCTTTAAACAGGGACC / 373-394 / sequencing
KSB6C02M 3R6** / GTGATATCTCCTTAAATAGAGACC / 371-394 / sequencing
SHAVM 3F6 / ATGCTTTCACATGTCGAAAG / 496-515 / sequencing
SHAVM 3R5 / TGGTCAATTACATCGGAATG / 1067-1086 / sequencing
SHAVM 3F5 / CCGATGTAATTGACCATATT / 1063-1082 / sequencing
SHAVM 3R4 / CAATACTTCTTAATGCTGGAC / 1718-1738 / sequencing
SHAVM 3F4 / CGCCATTTAACAGTGTCAGG / 1696-1715 / sequencing
SHAVM 3R3 / CAAATGGTATGCTAAAAAGTTCAC / 2402-2425 / sequencing
SHAVM 3F3 / GAACTTTTTAGCATACCATTTG / 2402-2423 / sequencing
SHAVM 3R2 / CTACAAACTGGTTAACATTTAC / 3050-3071 / sequencing
SHAVM 3F2 / CCATATACACAGCCAGTATTTAT / 3121-3143 / sequencing
SHAVM 3R1 / ACTGGTTTATATGGTTCAGT / 3604-3623 / sequencing
SHAVM 3F1 / CTCGGCATGATACATCATCA / 3726-3745 / sequencing

*The primers are also used for DOUV.

**The primer is specific for sequencing analysis of SHAV Japanese strains.

***The nucleotide positions given according to the numbering in the SATV OY-1/P/99 (AB698471), DOUV CSIRO150 (AB698478) and SHAV An5550 (AB698475).