Cytisus villosus from Northeastern Algeria is nodulated by genetically diverse Bradyrhizobium strains

Hadjira Ahnia, FaridaBoulila, Abdelghani Boulila, Karima Boucheffa, David Durán, Yasmina Bourebaba, Adouda Salmi, Juan Imperial, Tomás Ruiz-Argüeso, Luis Rey

Supplementary Material

Table S1.Distribution of fifty-one isolates of C. villosus in 16 RAPD electrophoretic patterns and accession numbers for sequences of a representative (in boldface) of each group. Public sequences from other strainsalso used in this work are included.

Patterns / Strains / Accession numbers
16S rDNA / atpD / glnII / recA / nodC
I / CTO1, CTS45, CTO56 / KF527961 / KF527945 / KF527929 / KF527913 / KF527977
II / CTO11, CTO8, CTO37, CTO15, CTO6, CTO7, CTO4 / KF527962 / KF527946 / KF527930 / KF527914 / KF527978
III / CTO13, CTO48, CTO27 / KF527963 / KF527947 / KF527931 / KF527915 / KF527979
IV / CTO21, CTO47, CTO45 / KF527964 / KF527948 / KF527932 / KF527916 / KF527980
V / CTO24, CTO39, CTO49, CTO50 / KF527965 / KF527949 / KF527933 / KF527917 / KF527981
VI / CTO29, CTS47, CTO19 / KF527966 / KF527950 / KF527934 / KF527918 / KF527982
VII / CTO33, CTO5 / KF527967 / KF527951 / KF527935 / KF527919 / KF527983
VIII / CTO41, CTO40 / KF527968 / KF527952 / KF527936 / KF527920 / KF527984
IX / CTO44, CTO32, CTO51, CTO52 / KF527969 / KF527953 / KF527937 / KF527921 / KF527985
X / CTO55, CTO42, CTO43, CTO53 / KF527970 / KF527954 / KF527938 / KF527922 / KF527986
XI / CTS7, CTS29 / KF527971 / KF527955 / KF527939 / KF527923 / KF527987
XII / CTS8, CTS1 / KF527972 / KF527956 / KF527940 / KF527924 / KF527988
XIII / CTS12, CTO17 / KF527973 / KF527957 / KF527941 / KF527925 / KF527989
XIV / CTS21, CTS20 / KF527974 / KF527958 / KF527942 / KF527926 / KF527990
XV / CTS24, CTS2, CTO20, CTS38 / KF527975 / KF527959 / KF527943 / KF527927 / KF527991
XVI / CTS57, CTO10, CTS10, CTO3 / KF527976 / KF527960 / KF527944 / KF527928 / KF527992
Azorhizobium caulinodans ORS 571 / - / - / - / - / AP009384
Bradyrhizobium arachidis CCBAU 051107T / HM107167 / - / - / - / HM107267
B. betae PL7HG1T / AY372184 / FM253129 / AB353733 / AB353734 / -
B. canariense CTFI47 / - / - / GU001610 / GU001591 / EU597858
B. canariense BTA-1T / AJ558025 / AY386739 / AY386765 / AY591553 / AJ560653
B. canariense CTAW31 / - / - / GU001597 / GU001578 / EU597847
B. canariense CTAW33 / - / - / GU001598 / GU001579 / EU597848
B. canariense CTFI26 / - / - / GU001606 / GU001587 / EU597856
B. canariense CTFI54 / - / - / GU001612 / GU001593 / EU597860
B. cytisi CTAW11T / EU561065 / GU001613 / GU001594 / GU001575 / EU597844
B. daqingense CCBAU 15774T / HQ231274 / HQ231289 / HQ231301 / HQ231270 / HQ231326
B. denitrificans LMG 8443T / X66025 / FM253153 / HM047121 / FM253196 / -
B. diazoeffieciens USDA110T / BA000040 / NP767080 / BA000040 / BA000040 / BA000040
B. elkanii USDA76T / U35000 / AY386758 / AY599117 / AM168342 / AM117554
B. huanghuaihaiense CCBAU 23303T / HQ231463 / HQ231682 / HQ231639 / HQ231595 / HQ231507
B. iriomotenseEK05T / AB300992 / AB300994 / AB300995 / AB300996 / AB301000
B. japonicum BGA-1 / - / - / - / - / HQ233213
B. japonicum CTAW56 / - / - / GU001602 / GU001583 / EU597851
B. japonicum CTFI32 / - / - / GU001608 / GU001589 / -
B. japonicum USDA6T / AB231927 / AJ294388 / AF169582 / AM168341 / AM117545
B. jicamae PAC68T / AY624134 / FJ428211 / FJ428204 / HM047133 / JX518552
B. lablabi CCBAU 23086T / GU433448 / GU433473 / GU433498 / GU433522 / JX518553
B. liaoningense LMG 18230T / AF208513 / AY386752 / AY386775 / AY591564 / FJ418727
B. oligotrophicum LMG 10732T / JQ619230 / JQ619232 / JQ619233 / JQ619231 / -
B. pachyrhizi PAC48T / AY624135 / FJ428208 / FJ428201 / HM047130 / AB573868
B. retamaeRo19T / KC247085 / KC247101 / KC247109 / KC247094 / KC247112
B. retamaeRs1 / - / - / - / - / KC247111
B. rifense CTAW71T / EU561074 / GU001617 / GU001604 / GU001585 / EU597853
B. yuanmingense CCBAU 10071T / AF193818 / AY386760 / AY386780 / AY591566 / AM117557
B. sp ISLU40 / - / - / - / - / HQ233219
B. sp ISLU101 / - / - / - / - / EF694757
Bosea thiooxidans DSM9653 T / NR041994 / - / - / - / -
Mesorhizobium loti MAFF303099 / - / - / - / - / BA000012
Rhizobium leguminosarum 3841 / - / - / - / - / AM236084
Sinorhizobium fredii 205 T / - / - / - / - / GU994072
Sinorhizobium meliloti 1021 T / - / AL591688 / AL591985 / AL591688 / AE006469

Fig. S1. M13 RAPD strain fingerprinting. Lanes are labelled with the name of the representative strain for each pattern. M: molecular weight marker ladder ranged from 3,000 to 100 bp

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