Čtvrtek 12. 10. 2017, 14.00 – 15.50, A11/132
Immuno-assay and metal stain development for application inLA-ICP-MS. Norbert JakubowskiHeike Traub, Larissa Mueller, Antje Herrmann; BAM Federal Institute for Materials Research and Testing, Richard-Willstaetter-Str. 11, 12489 Berlin, Germany;
We are using LA-ICP-MS to quantify metals in biological cells and thin cuts of tissues from various organs. Different applications will be discussed to demonstrate the state of the art of bioimaging to visualize the elemental distribution pattern in soft bio-materials (tissue, single cells) of metals and metal-tagged antibodies. For this purpose, different strategies for metal tagging will be discussed and first applications for detection of biomarkers in animal and human tissues will be presented.
In a first example, we have applied LA-ICP-MS to visualize the local distribution of proteins, which are used as bio-markers for neurodegenerative diseases. For this purpose, brain tissues from mice experiments have been stained by metal-tagged antibodies. House-keeping proteins serve as internal standards to overcome differences in protein expression. Additionally, ink-jet printing of metal doped inks onto the surface of tissue samples has been applied for drift corrections and quantification.
In a second example, we used specific metal-tagged antibodies for detection of prostate cancer and a micro tissue array for high throughput-screening of many patient samples using simultaneous detection of all relevant biomarkers.
In the next example application of antibodies for detection of metallothionines in eye lens tissues will be discussed.
Recently we have used our tagging strategies to apply metal tagged antibodies to determine the cell cycle of single cells, which is of interest for toxicological studies.
Finally future trends in mass cytometry imaging will be discussed.
Bundesanstalt für Materialforschung und -prüfung (BAM)
Dr. Norbert Jakubowski
Fachbereich 1.1 Anorganische Spurenanalytik
Richard-Willstätter-Straße 11
12489 Berlin
Die BAM ist eine Bundesoberbehörde im Geschäftsbereich des Bundesministeriums für Wirtschaft und Energie.