AVIHAI PERL January 2010



1957 Born in Haifa, Israel.

1975 High school education in "Hareali Haifa".

1976-1979 Military service.

Marital status: Married; 3 sons.

Home address: 6 Hahermon St., Rishon L'e Zion Israel, Tel: +972-3-9699431.

Working address: Dept. of Fruit Tree Sciences, Agricultural Research Organization, The Volcani Center, P.O.Box 6, Bet-Dagan, 50250, Israel. Tel: +972-3-9683825; Cell: +972-506-220025

Fax: +972-3-9669583, email:

University Education and Additional Training

1979-1981 B.Sc. in Life Sciences at the Hebrew University, Jerusalem.

1981-1983 M.Sc. in Agriculture at the Hebrew University, Jerusalem.

Title of thesis: Flowering induction and the in vitro propagation of Iris lortetii.

Supervision by: Prof. Meira Ziv.

1986-1990 Ph.D. in Plant Genetics, at the Weizmann Institute of Science, Rehovot

Title of thesis: Studies on the plasmone and on nuclear-

genome/plasmone compatibility in the

genus Solanum.

Supervision by: Prof. Esra Galun.

1991-1992 Postdoctoral position at the Weizmann Institute of Science, Rehovot, with Prof. Gad Galili.

Research Subject: Studies on the genetic regulation of lysine and threonine over-producing genes in transgenic potato.

1997 Sabbatical leave at Moet-Hennesy (LVMH), Paris, France,

with Dr. Michel Boulay.

Research subject: Recalcitrant grape transformation.

Positions Held and Academic Status

1990-1991 Research Associate, the Weizmann Institute of Science, Rehovot.

1992 to date Research Scientist at the ARO, The Volcani Center, Institute of Plant Science, Department of Fruit Tree Sciences.

1994 Promoted to Senior Scientist (Grade B).

2002  Promoted to Associate Principle Investigator (Grade A).

2000 to 2004 Head, Department of Ornamentals Horticulture.

2005 to 2008 Deputy Head, Institute of Plant Sciences.

AVIHAI PERL January 2010



1a. Perl, A., Aviv, D. and Galun, E. (1988).

Ethylene and in vitro culture of potato. Suppression of ethylene vastly improves protoplast yield, plating efficiency and transient expression of an alien gene.

Plant Cell Rep. 7: 403-406.

2a. Perl, A., Aviv, D. and Galun, E. (1990).

Protoplast-fusion derived Solanum cybrids: application and phylogenetic limitations.

Theor. Appl. Gene. 79: 632-640.

3a. Perl, A., Aviv, D. and Galun, E. (1990).

Protoplast-fusion derived CMS potato cybrids: Potential seed-parents for hybrid, true-potato-seeds.

J. of Heredity 81: 438-442.

4a. Perl, A., Aviv, D. and Galun, E. (1991).

Intergeneric transfer of oligomycin resistance from Nicotiana sylvestris to Solanum tuberosum by protoplast fusion.

Mol. Gen. Gene. 225: 11-16.

5a. Perl, A., Aviv, D. Willmitzer, L. and Galun, E. (1991).

In Vitro Tuberization in Transgenic Potatoes Harboring GUS Linked to a Patatin Promoter: Effects of Sucrose Levels and Photoperiods.

Plant Sci. 73: 87-95.

6a. Perl, A., Aviv, D. and Galun, E. (1991).

Production and analysis of interspecific cybrids in Solanum: Correlation of organelle transfer with a plastome dendogram.

Mol. Gen. Gene. 228: 193-200.

7a. Perl, A., Shaul, O. and Galili, G. (1992).

Regulation of lysine synthesis in transgenic potato plants expressing a bacterial dihydrodipicolinate synthase in their chloroplasts.

Plant Mol. Biol. 19: 815-823.

8a. Perl, A., Kless, H., Blumental, A., Galili, G. and Galun, E. (1992).

Improvement of plant regeneration and GUS expression in scutellar wheat calli by optimizing culture conditions and DNA-microprojectile delivery procedures.

Mol. Gen. Genet. 235: 279-284.

9a. Perl, A., Perl-Treves, R., Aviv, D., Shalgi, E., Malkin, S. and Galun, E. (1993).

Enhanced oxidative stress defense in transgenic potato expressing tomato Cu,Zn superoxide dismutases.

Theor. Appl. Genet. 85: 568-576.

10a. Perl, A., Shaul, O. and Galili, G. (1993).

Bacterial dihydrodipicolinate synthase and desensitized aspartate kinase: Two novel selectable markers for plant transformation relying on overproduction of lysine and threonine.

Bio/Technology 11: 715-718.

11c. Holland, D., Faltin, Z., Perl. A., Ben-Hayyim, G. and Eshdat, Y. (1994).

A novel plant glutathione peroxidase-like protein provides tolerance to oxidative radicals generated by paraquat in E. coli.

FEBS Letters 337: 52-55.

12a. Perl, A., Saad, S., Sahar, N. and Holland, D. (1995).

Establishment of long term embryogenic cultures of seedless Vitis vinifera cultivars - a synergistic effect of auxins and the role of abscisic acid.

Plant Sci. 104: 193-200.

13a. Perl, A., Lotan, O. Abu-Abeid , M. and Holland, D. (1996).

Establishment of an Agrobacterium mediated genetic transformation system for grape (Vitis Vinifera L.): The role of antioxidants during grape-Agrobacterium interaction.

Nature Biotechnology. 14: 624-628.

14d. Olesinski, A., Almon, E., Navot, N., Perl, A., Galun, G., Lucas, W.J. and Wolf, S. (1996).

Tissue-specific expression of a tobacco mosaic virus movement protein in transgenic potato plants alters plasmodesmal function and carbohydrate partitioning.

Plant Physiol 111: 541-550.

15c. Holland, N., Saad, S., Perl, A. and Holland, D. (1996).

Tentoxin sensitivity of citrus: cotyledons dependency of growth inhibition and reversibility of chlorosis.

J. Exp. Bot. 47: 837-842.

16b. Ben-Tzvi T. I., Perl, A. and Galili, G. (1996).

Lysine and threonine metabolism are subjected to complex patterns of regulation in Arabidopsis.

Plant Mol. Biol. 32: 727-734

17a. Perl, A., Gollop, R., Lipsky, A., Holland, D., Sahar, N., Or, E. and Elyasi, R. (1996).

Regeneration and transformation of grape (Vitis vinifera L.)

Plant Tiss. Cult. Biotechnol. 2: 187-193.

18d. Faltin, Z., Ben-Hayyim, G., Camoin, L., Perl, A., Beeor-Tzahar, T., Strosberg, A.D., Holland, D. and Eshdat, Y. (1998).

Non-selenium plant phspholipid hydroperoxide glutathione peroxidase is over-expressed under salt and oxidative stress.

Physiol. Plant. 104: 741-746.

19b. Radian-Sade, S., Perl, A., Edelbaum, O., Kuznetsova, L., Gafny, R., Sela, I. and Tanne, E. (2000).

Transgenic Nicotiana benthamiana and grapevine plants transformed with grapevine virus A (GVA) sequences.

Phytoparasitica 28: 79-86.

20d. Reuveni, M., Evenor, D., Artzi, B., Perl, A. and Erner, Y. (2001).

Decrease in vacuolar pH during petunia flower opening is reflected in the activity of tonoplast H+/ATPase.

J. Plant Physiol. 158: 991-998.

21a. Gollop, R., Farhi, S. and Perl, A. (2001)

Regulation of the leucoanthocyanidin dioxygenase gene expression in Vitis vinifera.

Plant Sci. 161: 579-588.

22a. Ginzberg, I., Perl, A., Wininger, S., Badani, H., Ovdat, N. and Kapulnik, Y. (2001).

Expression of strepavidin gene impedes growth and development of transgenic tomato plants.

Confidential reviewed.

23a. Gollop, R., Even, S., Colova-Tsolova V. and Perl, A. (2002).

Expression of the grape dihydroflavonol reductase gene and analysis of its promoter region.

J. Exp. Bot, 53: 1397-1409.

24a. Wang, Q., Gafny, R., Sahar, N., Mawassi, M., Tanne. E. and Perl, A. (2002)

Cryopreservation of grapevine (Vitis vinifera L.) embryogenic cell suspensions and subsequent plant regeneration by encapsulation-dehydration.

Plant Sci. 162: 551-558.

25a. Ginzberg, I., Perl, A., Genser, M., Wininger, S., Nemas,C. and Kapulnik Y. (2004).

Expression of streptavidin in tomato resulted in abnormal plant development that could be restored by biotin application.

J. Plant Physiol. 161: 611-620.

26b. J. Li, M. Jain, R. Vunsh, J. Vishnevetsky, U. Hanania, M. Flaishman, A. Perl, M. Edelman (2004).

Callus induction and regeneration in Spirodela and Lemna.

Plant Cell Rep. 22: 457-464.

27a. Wang, Q., Mawassi, M., Sahar, N., Li, P., Colova-Tsolova V., Gafny, R., Sela, I., Tanne, E. and Perl. A. (2004).

Cryopreservation of grapevine (Vitis spp.) embryogenic cell suspensions by encapsulation-vitrification.

Plant Cell Tissue Org. Cult. 77: 267-275.

28a. Hanania, U., Velcheva, M., Sahar, N. and Perl, A. (2004).

An improved method for the isolation of high quality DNA from Vitis vinifera.

Plant Mol. Biol. Rep. 22: 173-177.

29a. Velcheva, M., Faltin, Z., Flaishman, M., Eshdat, Y.,. and Perl, A. (2005).

A liquid culture system for Agrobacterium-mediated transformation of tomato (Licopersicon esculentum, L. Mill).

Plant Sci. 168: 121-130.

30a. Wang, Q., Li, P., Hanania, U., Sahar, N., Mawassi, M., Gafny, R., Sela, I., Tanne, E. And Perl. A. (2005).

Improvement of Agrobacterium-mediated transformation efficiency and transgenic plant regeneration of Vitis vinifera L. by optimizaing selection regimes and utilizing cryopreserved cell suspensions.

Plant Sci. 168: 565-571.

31a. Velcheva, M., Faltin, Z., Vardi, A., Eshdat, Y. and Perl, A. (2005).

Regeneration of Aloe Arborescence via somatic organogenesis from young inflorescences.

Plant Cell Tissue Org. Cult. 83: 293-301.

32b. Yancheva, S. D., Golubowicz, S., Yablowicz, Z., Perl, A. and Flaishman, M.A. (2005).

Efficient Agrobacterium-mediated transformation and recovery of transgenic plants from fig (Ficus carica L.).
Plant Sci. 168: 1433-1441.

33b. Yancheva, S. D., Shlizerman, L.A., Golubowicz, S., Yabloviz, Z., Perl, A.,
Hanania, U. and Flaishman, M.A. (2006).

Green fluorescent protein as a tool for improving Agrobacterium - mediated transformation of ‘Spadona’ pear (Pyrus communis L.).
Plant Cell Rep25: 183-189.

34a. Hanania, U., Velcheva, M., Or, E., Flaishman, M., Sahar, N. and Perl, A., (2007).

Silencing of Chaperonin 21 that was differentially expressed in inflorescence of seedless and seeded grapes, promoted seed abortion in tobacco and tomato fruits.

Transgenic Research 16: 515-525.

34b. Vunsh, R., Li, J., Hanania, U., Edelman, M., Flaishman, M., Perl, A., Wisniewski, J.P. and Freyssinet, G. (2007).

High expression of transgene protein in Spirodela.

Plant Cell Rep 26: 1511-1519.

35b. Rival, S., Wisniewski, J.P., Langlais, A., Kaplan, H., Freyssint, G., Vancannyet, G., Vunsh, R., Perl, A. and Edelman, M. (2008).

Spirodela (duckweed) as an alternative production system for pharmaceuticals: a case study, aprotinin.

Transgenic Research 17: 503-513.

36a. Hanania, U., Velcheva, M., Sahar, N., Flaishman, M., Or, E., Dgani, O. and Perl, A., (2009).

Suppression and over-expression of ubiquitin extension protein S27a affects proliferation and differentiation of Nicotiana benthamiana.

Plant Sci 176: 566-574.

37a. Hanania, U., Velcheva M., Sahar, N., Flaishman, M., Or, E., Dgani, O. and Perl, A. (2009).

The ubiquitin extension S27a protein is differently expressed in developing flower organs between the Thompson seedless and the Thompson seeded grape cultivars.

Plant Cell Rep 28: 1033-1042.

38b. Zhang, J.W., Ma, H.Q., Chen, S., Min Ji, M., Perl, A. and Chen, S.W. (2009).

Stress related proteins in embryogenic callus of Vitis vinifera L. cv. Cabernet Sauvignon – a proteomic approach.

Plant Sci 177: 103-113.

39a. Velcheva, M., Faltin, Z., Vardi, A., Hanania, U., Eshdat Y. and Perl, A. (2010).

Amberite XAD-4 beads and antioxidants enhance transformation and regeneration of Aloe Vera in suspension cultures.

(In Vitro Plant Cell Devel submitted)

40a. Vishnevetsky, J., Flaishman, M., Cohen, Y., Elad, I., Velcheva, M., Hanania, U. and Perl, A. (2010).

Improved tolerance toward fungal diseases in transgenic Cavendish banana (Musa spp. AAA group) cv. ‘Grand Nain’.

(Transgenic Research submitted)

41a.Faltin, Z., Velcheva, M., Tsapovetsky, M., Holland, D., Roeckel-Drevet, P., Handa, A., Abu-Abid, M., Friedman-Einat, M., Eshdat, Y. and Perl, A. (2010).

Glutathione peroxidase activity regulating ROS level is crucial for plant development during regeneration.

(Plant Physiol. submitted).

42b. Zhao, F., Chen, S., Perl, A. and Ma, H. (2010).

Differentially expressed epigenetic- related genes in embryogenic and non-embryogenic calli of grapevine (Vitis vinifera L.).

(J. Plant Physiol. Biochem. submitted)

43b. Zhanga, J., Zhaob, X., Perl, A., Chena, S. and Ma, H. (2010).

Proteomic analysis of differentially expressed proteins of vacuoles during grape berry development.

(J. Exp. Bot. submitted)


1c. Galun, E., Aviv, D., Breiman, A., Fromm, H., Perl, A. and Vardi, A. (1987).

Cybrids in. Nicotiana, Solanum and Citrus Isolation and characterization of plastome mutants, pre-fusion treatment, selection and analysis of cybrids.

In: Plant Molecular Biology, NATO Adv. Study Inst. (D. von Wettstein and N.H. Chua, eds.), Plenum Press pp 199-207.

2b. Galun, E., Perl, A. and Aviv, D. (1988).

Protoplast-fusion mediated transfer of male-sterility and other plastome-controlled traits. Application of plant cell and tissue culture.

In: Ciba Symp. Vol. 37. (J. Wiley and Sons eds.), pp 97-112.

3a. Galun, E., Aviv, D. and Perl, A. (1994).

Cybridization in potato.

In: Biotechnology in Agriculture and Forestry, Somatic Hybridization in Crop Improvement, (Y.P.S. Bajaj, ed.), Springer-Verlag, Berlin, Heidelberg, Vol. 27, pp. 167-182.

4d. Galili, G., Shaul, O., Perl, A. and Karchi, H. (1994).

Synthesis and accumulation of the essential amino acids lysine and threonine in seeds.

In: Seed development and germination, (H. Kigel, and G. Galili, eds.), Marcel Dekker, NY, pp. 811-832.

5a. Perl, A. and Eshdat, Y (1998).

DNA transfer and gene expression in transgenic grapes.

In: Biotechnology and Genetic Engineering Reviews, (M.P. Tombs ed.), Intercept Publishers UK, Vol. 15, pp. 365-386.

6a. Perl-Treves, R. and Perl, A. (2001).

Oxidative stress: an introduction.

In: Oxidative Stress in Plants. (M. Van Montagu and D. Inze, eds.), Harwood Academic Publishers, pp. 1-36.

7a. Colova-Tsolova, V., Perl, A., Krastanova, S., Tsvetkov, I. and Atanassov, A. (2001).

Genetically engineered grape for disease and stress tolerance.

In, Molecular Biology & Biotechnology of Grapevine, (K.A. Roubelakis-Angelakis, ed.), Kluwer Academic publishers, Dordrecht, pp. 411-432.

8a. Perl, A., Colova-Tsolova, V. and Eshdat, Y. (2004).

Agrobacterium-mediated transformation of grape embryogenic calli. In: Transgenic Crops of the World, Essential Protocols, (I. Curtis ed.), Kluwer Academic Publisher,Dordrecht, pp. 229-242.

9a. Wang, Q. and Perl, A. (2005).

Cryopreservation of embryogenic cell suspensions by encapsulation-vitrification.

In: Plant Cell Culture Protocols – Methods in Molecular Biology, (V.M. Loyola-Vargass, and F. Vázquez-Flota, eds.) Second Edition, Humana Press, pp. 77-86.

10a. Veltcheva, M., Svetleva, D., Petkova, S.P. and Perl, A. (2005).

In vitro regeneration and genetic transformation of common bean (Phaseolus vulgaris L.) – problems and progress.

Scientia Horticultureae 107:2-10.

11a. Wang, O., Perl, A. (2006).

Cryopreservation in floricultural crops.

In: Floriculture, Ornamentals and Plant Biotechnology, Vol. 1, (J.A. Teixeira de Silva, ed.) Global Science Books Ltd. London, UK, pp. 523-539.

12a. Perl, A., and Eshdat, Y. (2007).


In: Biotechnology in Agriculture and Forestry - Transgenic Crops V. Vol. 60. (E.C. Pua and M.R. Davey, eds.) Springer-Verlag, Berlin Heidelberg, pp. 189-208.

13a. Velcheva, M., Svetleva, D., Vidmar, J. and Perl, A. (2009).

In vitro plant regeneration and genetic transformation events in common bean (Phaseolus vulgaris L.).

In: Grain Legume Research, Tissue Culture, Biotechnology and Genetic Engineering, Vol. 1, (P. Sivakumar, and E. Ganam-Kokiladevi eds.), Bioscience Publications, India, pp. 121-131.

14a. Colova-Tsolova, V., Perl, A., Krastanova, S., Samuelian, S. and Atanassov, A. (2009).

Progress in genetic engineering of grapevine for disease & stress tolerance.

In: Grapevine molecular physiology & biotechnology, 2, (K.A. Roubelakis-Angelakis ed.), Springer, Netherlands, pp. 509-533.