Supplemental Table 1Ticks specimens classified as Ixodes scapularis

I.D. / Origin / Province / Infection
Status / Host / CO1
Sequence / 28S
Sequence / 16S
Sequence / 18S
Sequence
T006-12 / Aulac / NB / N / Dog
T013-12 / Port Elgin / NB / N / Cat
T045-12 / Dorchester / NB / N / Human
T117-12 / Dorchester / NB / N / Cat
T122-12 / Saint John / NB / N / Dog
T130-12 / Pictou / NS / N / Dog / I. scapularis / I. scapularis / I. scapularis / I. scapularis
T133-12 / New Glasgow / NS / P / Dog
T149-12 / Dorchester / NB / N / Dog
T154-12 / Saint Stephan / NB / N / Dog
T162-12 / Port Howe / NB / N / Cat
T164-12 / Riverview / NB / N / Cat
T169-12 / Dieppe / NB / N / Dog
T172-12 / Aulac / NB / N / Dog
T182-12 / St. Andrews / NB / N / Dog / I. scapularis / I. scapularis / ND / ND
T200-12 / Dieppe / NB / N / Human
T208-12 / St. Andrews / NB / N / Dog
T227-12 / Little Forks / NS / N / Cat
T229-12 / Sackville / NB / N / Cat
T246-12 / Merigomish / NS / N / Human
T253-12 / St. Anne / NB / N / N/A
T263-12 / Dufferin / NB / N / Dog
T264-12 / Unknown / NB / N / Cat
T265-12 / Todd's Point / NB / N / Dog
T271-12 / Sackville / NB / N / Dog
T299-12 / Hampton / NB / N / Dog
T315-12 / Sackville / NB / N / Dog
T331-12 / Hampton / NB / N / Dog
T332-12 / Saint John / NB / N / Dog
T337-12 / Shediac / NB / N / Dog
T338-12 / Shediac / NB / N / Cat
T363-12 / Moncton / NB / P / Dog
T379-12 / Sackville / NB / N / N/A
T031-13 / St. Stephan / NB / N / Dog
T064-13 / Rothesay / NB / N / Dog
T067-13 / St. Charles / NB / N / Dog
T072-13 / New Glasgow / NS / P / Human
T085-13 / Fundy / NB / P / Dog
T086-13 / Moncton / NB / P / Dog
T088-13 / Sackville / NB / N / Dog
T089-13 / Saint John / NB / P / Dog
T092-13 / Riverview / NB / N / Human / I. scapularis / I. scapularis / ND / ND
T093-13 / Riverview / NB / N / Dog
T098-13 / Dieppe / NB / N / Dog
T100-13 / Hampton / NB / N / Dog
T112-13 / Port Rowan / Ontario / N / Human / I. scapularis / I. scapularis / I. scapularis / I. scapualris
T113-13 / St.Charles / NB / N / Cat / I. scapularis / I. scapularis / I. scapularis / I. scapualris
T127-13 / Saint John / NB / N / Cat
T142-13 / South / NB / N / Human
T144-13 / Moncton / NB / N / Dog / I. scapularis / I. scapularis / I. scapularis / I. scapualris
T156-13 / Fredericton / NB / N / Dog
T161-13 / Tracy / NB / N / Dog
T164-13 / Little Harbour / NS / P / Human / I. scapularis / I. scapularis / ND / ND
T166-13 / Merigomish / NS / P / Human
T170-13 / Dieppe / NB / N / Dog
T173-13 / Moncton / NS / P / Dog
T203-13 / Center Village / NB / N / Dog
T209-13 / Amherst / NS / N / Human
T223-13 / Hampton / NB / N / Human
T257-13 / Sackville / NB / N / Dog
T266-13 / Sackville / NB / N / Cat
T267-13 / Sackville / NB / N / Cat
T277-13 / Quispamsis / NB / P / Human
T278-13 / Saint John / NB / N / Dog
T279-13 / Amherst / NS / N / Dog
T280-13 / Amherst / NS / N / Dog
T283-13 / Gage / NB / N / Dog
T284-13 / Gage / NB / N / Dog
T293-13 / Jolicure / NB / N / Dog
T295-13 / Aulac / NB / P / Dog
T300-13 / Bath / NB / N / Dog
T301-13 / WoodPoint / NB / N / Dog
T307-13 / Hampton / NB / N / Cat
T314-13 / Gagetown / NB / N / Dog
T315-13 / Gagetown / NB / N / Dog
T316-13 / Gagetown / NB / N / Dog
T317-13 / Gagetown / NB / N / Dog
T318-13 / Gagetown / NB / P / Dog / I. scapularis / I. scapularis / ND / ND
T319-13 / Amherst / NS / N / Horse
T320-13 / Saint John / NB / N / Dog
T322-13 / Brookdale / NS / N / Cat
T324-13 / Amherst / NS / N / Cat / I. scapularis / I. scapularis / I. scapularis / I. scapualris
T331-13 / Hampton / NB / N / Dog
T336-13 / Bathurst / NB / N / Dog
T342-13 / Rothesay / NB / N / Dog
T343-13 / Rothesay / NB / N / Dog
T347-13 / Amherst / NS / N / Dog
T355-13 / Saint John / NB / P / Dog
T502-13 / Amherst / NS / N / Cat

ND= not done

Supplemental Table 2Tick specimens classified as Morphologically Ambiguous

I.D. / Origin / Province / Infection Status / Host / CO1
Sequence / 28S
Sequence / 16S
Sequence / 18S
Sequence
T016-12 / Sackville / NB / N / Cat / I. cookei / I. cookei / I. cookei / I.cookei
T025-12 / Moncton / NB / N / Groundhog / I. cookei / I. cookei / I. cookei / I.cookei
T026-12 / Moncton / NB / P / Groundhog / I. cookei / I. cookei / I. cookei / I.cookei
T033-12 / Aulac / NB / N / Human / I. cookei / I. cookei / I. cookei / I.cookei
T034-12 / Aulac / NB / N / Human / I. cookei / I. cookei / I. cookei / I.cookei
T036-12 / Aulac / NB / N / Human / I. cookei / I. cookei / I. cookei / I.cookei
T039-12 / Sackville / NB / N / Cat / I. cookei / I. cookei / I. cookei / I.cookei
T041-12 / Sackville / NB / P / Cat / I. cookei / I. cookei / I. cookei / I.cookei
T042-12 / Dorchester / NB / P / Cat / I. cookei / I. cookei / I. cookei / I.cookei
T043-12 / Dorchester / NB / N / Human / I. cookei / I. cookei / I. cookei / I.cookei
T046-12 / Sackville / NB / N / Cat / I. cookei / I. cookei / I. cookei / I.cookei
T049-12 / Dorchester / NB / N / Cat / I. cookei / I. cookei / I. cookei / I.cookei
T050-12 / Dorchester / NB / N / Cat / I. cookei / I. cookei / I. cookei / I.cookei
T056-12 / Cole's Island / NB / N / Human / I. cookei / I. cookei / I. cookei / I.cookei
T058-12 / Sackville / NB / N / Cat / I. cookei / I. cookei / I. cookei / I.cookei
T060-12 / Dorchester / NB / N / Cat / I. cookei / I. cookei / I. cookei / I.cookei
T072-12 / Saint John / NB / N / Dog / I. scapularis / I. scapularis / I. scapularis / I. scapularis
T073-12 / St. Paul / NB / N / Dog / I. cookei / I. cookei / I. cookei / I.cookei
T088-12 / Dorchester / NB / N / Cat / I. cookei / I. cookei / I. cookei / I.cookei
T105-12 / Cornhill / NB / P / Dog / I. cookei / I. cookei / I. cookei / I.cookei
T149-13 / Dorchester / NB / P / Human / I. cookei / I. cookei / I. cookei / I.cookei
T247-13 / Sackville / NB / N / Dog / I. cookei / I. cookei / I. cookei / ND
T291-13 / Sackville / NB / N / Dog / I. scapularis / I. scapularis / I. scapularis / I. scapularis
T299-13 / Bathurst / NB / N / Dog / I. cookei / I. scapularis / I. cookei / ND
T300-13 / Bathurst / NB / N / Dog / I. cookei / I. cookei / I. cookei / I.cookei
T327-13 / Saint John / NB / N / Dog / I. scapularis / I. scapularis / I. scapularis / I. scapularis
T328-13 / New Glasgow / NS / Unknown / Dog / I. scapularis / I. scapularis / I. scapularis / ND
T393-13 / Point Verte / NB / N / Dog / I. scapularis / I. scapularis / I. scapularis / I. scapularis
T413-13 / Sackville / NB / N / Dog / I. scapularis / I. scapularis / I. scapularis / I. scapularis
T414-13 / Sackville / NB / N / Dog / ND / ND / ND / ND
T426-13 / Grand Bay / NB / N / Dog / ND / ND / ND / ND
T465-13 / Dieppe / NB / N / Dog / ND / ND / ND / ND
T498-13 / Rothesay / NB / N / Dog / I.scapularis / I. scapularis / I. scapularis / I. scapularis
T510-13 / Dieppe / NB / N / Dog / I.scapularis / I. scapularis / I. scapularis / I. scapularis
T506-13 / Sackville / NB / N / Dog / I.scapularis / I. scapularis / I. scapularis / I. scapularis

ND= not done

Supplemental Table 3 Tick specimens identified as I. cookei

I.D. / Origin / Province / Infection Status / Host / CO1 / 28S / 16S / 18S
T011-12 / Amherst / NS / N / Cat / I. cookei / I. cookei / I. cookei / I.cookei
T003-12 / Sackville / NB / Unknown / Cat
T008-12 / Sackville / NB / N / Human
T014-12 / Sackville / NB / N / Cat
T017-12 / Moncton / NB / N / Dog
T022-12 / Sackville / NB / P / Cat
T032-12 / Halifax / NS / N / Human
T039-12 / Sackville / NB / N / Cat
T090-12 / Dorchester / NB / N / Cat
T103-12 / Hillsborough / NB / N / Dog
T209-12 / Bathurst / NB / N / Dog
T211-12 / Gallagher Ridge / NB / N / Dog
T255-12 / Hillcrest / NB / N / Dog
T059-13 / Frosty Hollow / NB / N / Dog
T077-13 / St. Charles / NB / N / Cat / I. cookei / I. cookei / I. cookei / I.cookei
T078-13 / St. Charles / NB / N / Cat
T079-13 / St. Charles / NB / N / Cat
T080-13 / St. Charles / NB / N / Cat / I. cookei / I. cookei / I. cookei / I.cookei
T081-13 / St. Charles / NB / N / Cat / I. cookei / I. cookei / I. cookei / I.cookei
T090-13 / Melrose / NB / N / Cat
T095-13 / Aulac / NB / N / Dog
T096-13 / Saint John / NB / N / Cat
T097-13 / Aulac / NB / N / Dog
T103-13 / Melrose / NB / N / Cat
T105-13 / Sackville / NB / N / Cat
T110-13 / Aulac / NB / N / Dog
T138-13 / Sackville / NB / N / Cat / I. cookei / I. cookei / I. cookei / I.cookei
T147-13 / Dorchester / NB / P / Cat
T148-13 / Dorchester / NB / N / Cat
T151-13 / Sackville / NB / N / Cat / I. cookei / I. cookei / I. cookei / I.cookei
T157-13 / Heath Steele Mine / NB / N / Human / I. cookei / I. cookei / I. cookei / I.cookei
T162-13 / Mettigonish / NS / N / Human
T188-13 / Sackville / NB / N / Cat
T207-13 / Fredericton / NB / N / Cat / I. cookei / I. cookei / I. cookei / I.cookei
T209-13 / Amherst / NS / N / Human
T216-13 / Saint John / NB / N / Dog
T239-13 / Dorchester / NB / N / Cat
T247-13 / Sackville / NB / N / Dog
T248-13 / Sackville / NB / N / Cat
T249-13 / Sackville / NB / N / Cat
T269-13 / Prince William / NB / N / Dog
T270-13 / Cocagne / NB / N / Cat
T271-13 / Cocagne / NB / N / Cat / I. cookei / I. cookei / I. cookei / I.cookei
T359-13 / Janeville / NB / N / Dog
T366-13 / Hampton / NB / N / Cat
T367-13 / Hampton / NB / N / Cat
T419-13 / Penn, NB / NB / N / Cat / I. cookei / I. cookei / I. cookei / I.cookei
T420-13 / Pennfield / NB / N / Cat
T421-13 / Pennfield / NB / N / Cat
T428-13 / Sackville / NB / N / Dog