Table S1. Bacterial strains and plasmids used in the study.

Designation / Relevant characteristics / Source
Strains
A. tumefaciens
GV3101 / Disarmed A. tumefaciens strain, RifR / Van Larebeke et al. 1974
31::WB6 / GV3101 carrying pGWB6, KnR, RifR / This study
AG::2201 / AGL-1 carrying pCAMBIA2201, CmR, RifR / Moore, G.a
AG::2202 / AGL-1 carrying pCAMBIA2202, CmR, RifR / Moore, G.a
31::Gf1_2 / GV3101 carrying pGavrGf1_2, KnR, RifR / This study
31::Gf1_5 / GV3101 carrying pGavrGf1_5, KnR, RifR / This study
AvrGf1::N13 / GV3101 carrying p5N13, KnR, RifR / This study
AvrGf1::N116 / GV3101 carrying p5N116, KnR, RifR / This study
AvrGf1::C7 / GV3101 carrying p5C7, KnR, RifR / This study
AvrGf1::C83 / GV3101 carrying p5C83, KnR, RifR / This study
AvrGf1::N13* / GV3101 carrying p2N13, KnR, RifR / This study
AvrGf1::N116* / GV3101 carrying p2N116, KnR, RifR / This study
AvrGf1::C7* / GV3101 carrying p2C7, KnR, RifR / This study
AvrGf1::C83* / GV3101 carrying p2C83, KnR, RifR / This study
E. coli
DH5 / FrecA80dlacZM15 / Invitrogen
Plasmids
pENTR-D / Entry Vector / Invitrogen
pGWB2 / Binary vector for overexpression using CaMV35S promoter / Nakagawa et al. 2007
pGWB5 / Binary gfp expression vector, contains 35S promoter upstream of attR1-CmR-ccdB-attR2- sgfp / Nakagawa et al. 2007
pGWB6 / For N-fusion with CaMV35S promoter / Nakagawa et al. 2007
pL799 / X. citri strain AwavrGf1 fragmentin pLAFR3 / Rybak et al. 2009
pGavrGf1_2 / avrGf1 full sequence in pGWB2, KnR / This study
pGavrGf1_5 / avrGf1 full sequence in pGWB5, KnR / This study
pEavrGf1 / avrGf1 full sequence in pENTR-D, KnR / This study
pEN13 / 13 a.a.b deleted, after ATG from avrGf1in pENTR-D, KnR / This study
pEN116 / 116 a.a.b deleted, after ATG from avrGf1in pENTR-D, KnR / This study
pEC7 / 525 to 532 a.a.b were deleted in the C-terminus with the stop codon in pENTR-D, KnR / This study
pEC83 / 449 to 532 a.a.b were deleted in the C-terminus with the stop codon in pENTR-D, KnR / This study
p5N13 / 13 a.a.b deleted, after ATG from avrGf1in pGWB5, KnR / This study
p5N116 / 116 a.a.b deleted, after ATG from avrGf1in pGWB5 / This study
p5C7 / 525 to 532 a.a.b were deleted in the C-terminus with the stop codon in pGWB5 / This study
p5C83 / 449 to 532 a.a.b were deleted in the C-terminus with the stop codon in pGWB5 / This study
p2N13 / 13 a.a.b deleted, after ATG from avrGf1in pGWB2 / This study
p2N116 / 116 a.a.b deleted, after ATG from avrGf1in pGWB2 / This study
p2C7 / 525 to 532 a.a.b were deleted in the C-terminus with the stop codon in pGWB2 / This study
p2C83 / 449 to 532 a.a.b were deleted in the C-terminus with the stop codon in pGWB2 / This study

a Moore, G. University of Florida, Gainesville, FL, U.S.A.

b Amino acids (a.a.).

1