Title: Taxonomically restricted genes of Craterostigma plantagineum are modulated in their expression during dehydration and rehydration
Journal: Planta
Valentino Giarola, Stephanie Krey, Anneke Frerichs and Dorothea Bartels
Author for correspondence: Dorothea Bartels
Institute of Molecular Physiology and Biotechnology of Plants (IMBIO), University of Bonn, Kirschallee 1, D-53115 Bonn, Germany
Tel: +49 228 73 2070
Email:
Table S1 Primers used in this study
5’ and 3’-end cloning / Sequence (5’ to 3’) / Restriction siteZAP_F2 / CAGGAAACAGCTATGAACC / -
CpCRP1_R / GCGATGAGTTTACCAGCACA / -
CpEDR1_R1 / GGACGATCTTGCGATGAAGT / -
CpEDR1_R2 / TCCTTTGCCTTCTCCTTGAG / -
Protein overexpression / Sequence (5’ to 3’) / Restriction site
CpCRP1_CDS_F / CCATGGCCCACGAAATGGAAAAC / NcoI
CpCRP1_CDS_R / CTCGAGGCCTCTTATACCGCAGCAAAC / XhoI
CpEDR1_CDS_F / CCATGGGGCTCGCCGCAG / NcoI
CpEDR1_CDS_R / CTCGAGAGGACTTTTTACTGCCT / XhoI
Protein localization / Sequence (5’ to 3’) / Restriction site
CpCRP1_GFP_F1 / ACCCACCATGGCTCAAAG / NcoI
CpCRP1_ GFP_F2 / CCATGGCCCACGAAATGGAAAAC / NcoI
CpCRP1_GFP_R1 / AGGGCATCCCATGGCG / NcoI
CpCRP1_GFP_R2 / GTTTTCCATTTCGTGGGCCATGG / NcoI
CpEDR1_GFP_F1 / CGGACCATGGCAATATCCGCCGTC / NcoI
CpEDR1_GFP_F2 / GCTCGCCATGGCTCTAATCTCATC / NcoI
CpEDR1_GFP_R1 / CAAGCCATGGGACTTTTTACTGCCTC / NcoI
CpEDR1_GFP_R2 / AGAGCCATGGCGAGCCCAGCTAGC / NcoI
RT-PCR / Sequence (5’ to 3’) / Restriction site
CpCRP1_RT_F / CAGCATAAAGCGCCAAGTTC / -
CpCRP1_RT_R / GCGATGAGTTTACCAGCACAG / -
LbCRP1_RT_F / AAGACCAATGTTCCCGTCAA / -
LbCRP1_RT_R / CTCGTTTTCAGTGGATGTGC / -
LsCRP1_RT_F / CAAGGTCCCCGTCAATAGG / -
LsCRP1_RT_R / TGGCATTATTATGTTATGGTGAGTG / -
CpEDR1_RT_F / TTGAACCGAGGGACGATG / -
CpEDR1_RT_R / GTTTAACGTTGCAAGGATTGTCTC / -
LbEDR1_RT_F / ACAAGGCGTTGCCTAAATTG / -
LbEDR1_RT_R / TTTCTCGATCACATCCCACA / -
LsEDR1_RT_F / AAGAACGATGCACCCAAATC / -
LsEDR1_RT_R / AGGATGTTCTCGATCCCAAC / -
CpEIF5A_RT_F / CATCAATCGTGAAGGAGAGGA / -
CpEIF5A_RT_R / CTAAACCTTGGCAGCAGCAC / -
LbEIF5A_RT_F / GGGAAAGGACCTTGTTGTGA / -
LbEIF5A_RT_R / TGGGCTCATTACTCCACTGA / -
LsEIF5A_RT_F / TGGGTGGTTTTTATCAATGTCC / -
LsEIF5A_RT_R / GGTTGAGTGATAACACAGTAGCAAA / -
RT-qPCR / Sequence (5’ to 3’) / Restriction site
CpCRP1_QRT_F / CGTCACGGTGAGAAGTCGT / -
CpCRP1_QRT_R / GCGATGAGTTTACCAGCACAG / -
CpEDR1_QRT_F / AGGCAGTAAAAAGTCCTCGGAC / -
CpEDR1_QRT_R / GTTTAACGTTGCAAGGATTGTCTC / -
CpACT_QRT_F / CGATTTTGGCATCTCTCAGC / -
CpACT_QRT_R / AGCATTTTCTGTGAACAATCGAC / -
RT-qPCR / Sequence (5’ to 3’) / Restriction site
CpEF1α_QRT_F / TCGTATCTGCTTTTGCATTGAGT / -
CpEF1α_QRT_R / ACGAACAGCAACGACTCACA / -
CpEIF5A_QRT_F / CATCAATCGTGAAGGAGAGGA / -
CpEIF5A_QRT_R / CTAAACCTTGGCAGCAGCAC / -
CpGAPDH_QRT_F / GGCTTCGATTCGGATCTTCT / -
CpGAPDH_QRT_R / AGCCCACAAATATATCAAGAACTCA
CpTKT3_QRT_F / AGGTGAAAAAGGAGTGAAAATGG
CpTKT3_QRT_R / GTCTGCTGCAAACATGAGAAAA / -
CpYLS8_QRT_F / TCATCCGTCTCCTCAGGTGT / -
CpYLS8_QRT_R / TCAACAGCACAAAAGCATCAA / -