Supplemental Table S1. Animal characteristics and in vivo MR data obtained in wild-type (WT) and long-chain acyl-CoA dehydrogenase (LCAD) knockout (KO) mice without and with (+) carnitine supplementation.

Baseline / Week 7 / Week 9
WT / KO / WT / KO / WT+ / KO+ / WT / KO / WT+ / KO+
Age (days) / 37.6 / ± / 2.3 / 39.8 / ± / 2.4 / 53.8 / ± / 1.0 / 52.7 / ± / 3.3 / 51.4 / ± / 1.2 / 55.4 / ± / 3.2 / 67.9 / ± / 4.1 / 66.7 / ± / 2.1 / 67.5 / ± / 2.0 / 69.5 / ± / 2.0
Body weight (g) / 19.8 / ± / 2.1 / 20.6 / ± / 2.3 / 24.0 / ± / 1.1 / 23.2 / ± / 1.2 / 22.1 / ± / 1.2 / 23.6 / ± / 2.0 / 25.3 / ± / 1.3 / 25.3 / ± / 1.3 / 24.7 / ± / 1.2 / 25.5 / ± / 1.4
Heart mass (mg) / 105.8 / ± / 7.8 / 130.2 / ± / 12.9 / 104.7 / ± / 8.1 / 130.6 / ± / 9.8
MRI
LV mass (mg) / 75.1 / ± / 7.4 / 91.7 / ± / 15.0 / 96.1 / ± / 9.5 / 103.2 / ± / 11.6 / 87.2 / ± / 5.9 / 106.1 / ± / 10.2 / 99.7 / ± / 12.3 / 117.4 / ± / 12.0 / 95.5 / ± / 8.6 / 120.9 / ± / 8.9
LVM/BW (mg/g) / 3.81 / ± / 0.27 / 4.44 / ± / 0.52 / 4.05 / ± / 0.38 / 4.45 / ± / 0.32 / 3.93 / ± / 0.29 / 4.50 / ± / 0.25 / 3.99 / ± / 0.33 / 4.63 / ± / 0.39 / 3.84 / ± / 0.22 / 4.75 / ± / 0.19
EDV (μL) / 48.6 / ± / 8.2 / 52.3 / ± / 6.3 / 59.6 / ± / 9.9 / 59.8 / ± / 6.0 / 57.5 / ± / 8.3 / 59.0 / ± / 7.7 / 58.3 / ± / 10.9 / 63.2 / ± / 8.5 / 60.4 / ± / 8.2 / 59.9 / ± / 4.8
ESV (μL) / 13.5 / ± / 3.4 / 16.1 / ± / 3.1 / 17.6 / ± / 3.3 / 18.9 / ± / 3.9 / 17.7 / ± / 3.3 / 19.6 / ± / 5.9 / 18.5 / ± / 3.7 / 20.3 / ± / 5.2 / 19.7 / ± / 4.5 / 18.3 / ± / 3.8
SV (μL) / 35.0 / ± / 5.5 / 36.2 / ± / 3.8 / 42.0 / ± / 7.9 / 40.8 / ± / 3.2 / 39.8 / ± / 5.5 / 39.4 / ± / 4.0 / 39.8 / ± / 7.4 / 43.0 / ± / 4.3 / 40.6 / ± / 5.1 / 41.6 / ± / 2.2
EF (%) / 72.3 / ± / 4.0 / 69.3 / ± / 3.0 / 70.4 / ± / 3.7 / 68.6 / ± / 4.3 / 69.3 / ± / 2.8 / 67.2 / ± / 6.1 / 68.4 / ± / 1.7 / 68.3 / ± / 4.9 / 67.5 / ± / 4.6 / 69.7 / ± / 4.1
1H-MRS
TG -CH2- (% water) / 0.59 / ± / 0.27 / 0.86 / ± / 0.25 / 0.70 / ± / 0.18 / 0.84 / ± / 0.28 / 0.47 / ± / 0.17 / 0.74 / ± / 0.19 / 0.59 / ± / 0.19 / 1.03 / ± / 0.45 / 0.55 / ± / 0.16 / 0.63 / ± / 0.27

Data are expressed as mean ± SD. LVM, left ventricular mass; BW, body weight; EDV, end-diastolic volume; ESV, end-systolic volume; SV, stroke volume; EF, LV ejection fraction; TG -CH2-, myocardial triglyceride methylene signal relative to the total water signal.

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Supplemental Figure S1.

Supplemental Figure S1. Contributions of fatty acid species relative to the total myocardial fatty acid content in wild-type (WT) and long-chain acyl-CoA dehydrogenase (LCAD) knockout (KO) mice without and with (+) four weeks of carnitine supplementation. General genotype effect: †, P < 0.05; ††, P < 0.01; †††, P < 0.001.

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