Name:______

OBSERVING POND WATER PROTISTS and ANIMALS

Safety – wear gloves.

Prepare each organism for observation as instructed if you are the first observer.

Be sure to observe each organism for 2 – 3 minutes while completing your notes.

The last person at each station at the end of class will clean up each station as specified on the instruction card.

Do NOT wash any organism down the drain, wipe all slides with paper towel and then wash with soap and water and rinse.

Wash your hands with soap and water when you are done!

Name
Write the scientific name / Description
Describe colour, size, shape / Motion
Describe speed, direction, cilia, flagella, flowing cytoplasm / Sketch
On the diagram underline the parts you can see
1. Planaria /
Name
Write the scientific name / Description
Describe colour, size, shape / Motion
Describe speed, direction, cilia, flagella, flowing cytoplasm / Sketch
On the diagram underline the parts you can see
2. Vinegar Eels / - move super fast
- watch the screen carefully for a few minutes and you should see some flash past /
3. Amoeba /
Name
Write the scientific name / Description
Describe colour, size, shape / Motion
Describe speed, direction, cilia, flagella, flowing cytoplasm / Sketch
On the diagram underline the parts you can see
4. Hydra /
5. Paramecium /
Name
Write the scientific name / Description
Describe colour, size, shape / Motion
Describe speed, direction, cilia, flagella, flowing cytoplasm /
6. Daphnia
7. Euglena /

05_OBSERVING_PROTISTS_and_Animals_lab.doc

Name:______

Station 1 - Brown Planaria

Species: tigrina

Genus: Hydra

Family: Hydridae

Order: Hydroida

Class: Hydrozoa

Phylum: Cnidaria

Kingdom: Animalia

Set up

- gently micropipette one planaria into the well of an immersion slide

- avoid using too much water, pipette water back into container

Observe

- observe at low power

- shut off the light when not observing to keep from overheating and killing the organism

Clean Up

- gently push the planaria back into the container if it is still alive

-wipe slide and cover slip dry

- throw paper towel in garbage

- wash slide with soap, rinse well

- return slide and cover slip to cart

- pack up microscope as instructed

Station 2 – Vinegar Eels

Species: aceti

Genus: Turbatrix

Family: Panagrolaimidae

Order: Rhabditida

Class: Secernentea

Phylum: Nematoda

Kingdom: Animalia

Set up

- Pipette from the bottom of the container

- put 2 drops on a slide and put the rest back in the container

- put a cover slip on the slide

- focus on low power

Observation

- observe on medium power, centre sample on slide

- observe on high power

- keep light turned off when not observing

Clean Up

-wipe slide and cover slip dry

- throw paper towel in garbage

- wash slide with soap, rinse well

- return slide and cover slip to cart

- pack up microscope as instructed

Station 3 –Ameoba

Species: proteus

Genus: Amoeba

Family: Amoebidae

Order: Labosa

Class: Protozoa

Phylum: Sarcomastigota

Kingdom: Protozoa

Set up

- gently micropipette 1-2 drops of liquid from the bottom of the container onto a slide

- put the rest back in the container

- place on a cover slip

Observation

- be sure to focus through all the dimensions of the ameoba on low power

- shut off the light when not observing to keep from overheating and killing the organism

- note there are paramecium and other amoeba food in the culture

Clean Up

-wipe slide and cover slip dry

- throw paper towel in garbage

- wash slide with soap, rinse well

- return slide and cover slip to cart

- pack up microscope as instructed

Station 4 – Hydra

Species: viridissima

Genus: Hydra

Family: Hydridae

Order: Hydroida

Class: Hydrozoa

Phylum: Cnidaria

Kingdom: Animalia

Set up

- they are stuck to the bottom of the container

- very gently poke one off the bottom and suck it up into a micropipette

- avoid using too much water

- put one onto a slide and cover with a slip

- avoid using too much water

Observation

- observe on low power

- shut off the light when not observing to keep from overheating and killing the organism

Clean Up

-wipe slide and cover slip dry

- throw paper towel in garbage

- wash slide with soap, rinse well

- return slide and cover slip to cart

- pack up microscope as instructed

Station 5 – Paramecium

Species: caudatum

Genus: Paramecium

Family: Parameciidae

Order: Hymenostomatida

Class: Ciliatea

Phylum: Ciliophora

Kingdom: Protozoa

Set up

- suck up a small sample from the top of the container

- put 2 drops onto a slide and add a tiny microdrop of quietening solution

- do NOT contaminate the quietening solution micropipetter by touching the slide.

- put on a cover slip

Observation

- focus on low power, centre and observe on medium power

Clean Up

-wipe slide and cover slip dry

- throw paper towel in garbage

- wash slide with soap, rinse well

- return slide and cover slip to cart

- pack up microscope as instructed

Station 6 –Daphnia

Species: magna

Genus:Daphnia

Family: Daphniidae

Order: Cladocera

Class: Branchiopoda

Phylum: Arthropoda

Kingdom: Animalia

Set up

- expel air from micropipette before putting it in container

- watch one daphnia

- suck daphnia into pipette

- put into the well of immersion slide, remove excess water

Observation

- use low power then medium power

- keep light off when not observing to keep from overheating the daphnia

- if the daphnia is not alive then follow clean up and set up instructions to observe another daphnia

Clean Up

-wipe slide and cover slip dry

- throw paper towel in garbage

- wash slide with soap, rinse well

- return slide and cover slip to cart

- pack up microscope as instructed

Station 7–Euglena

Species: gracilis

Genus:Euglena

Family: Euglenaceae

Order: Euglenales

Class: Euglenida

Phylum: Euglenozoa

Kingdom: Protozoa

Set up

- put two drops from the bottom of the container on a slide and cover with a slip

Observation

- focus on low power, look for tiny green dots, centre slide

- use medium power and focus through all levels, then centre and try high power

- turn off light when you are not looking

Clean Up

-wipe slide and cover slip dry

- throw paper towel in garbage

- wash slide with soap, rinse well

- return slide and cover slip to cart

- pack up microscope as instructed

05_OBSERVING_PROTISTS_and_Animals_lab.doc