Setting up to Capture Fluorescent Images Using the DP30

Olympus BX51 Microscope

Switch on the mercury bulb if fluorescence is required. Black rocker switch on the U-RFL-T power supply box.

If Brightfield is required switch on the black rocker switch on the right hand side of the microscope.

Switch on the PC you require. This will depend on what camera you are going to use. We have two PCs attached to this microscope. For the DP30 camera ( monochrome 12 bit camera) switch on the DP30 PC and select the green button. For the DP71 Camera ( RGB colour camera) switch on the DP71 PC and select the orange button.

Setting up to capture fluorescent images using the DP30.

Place your slide on the microscope . Make sure the light is going to the eyepieces by pushing the black lever on the right side of the eyepieces all the way in. Top lever for DP30 Bottom lever for DP71

Select the fluorescent filter required for your sample. (Position 2 NU-DAPI, Position 3 GFP, Position 4 RFP position 5 if looking at H+E stained tissue samples ).

Double click on the CellF icon on the desktop to open the software.

Change the light path to the camera by pulling out the correct black lever for the camera you are using on the right hand side of the eyepieces. Top lever for DP30 bottom lever for DP71.

When you are focused on the image. Click on the still camera icon on the top menu to grab an image.

The image will be held in the image manager window for you to save. Do not display more than 20 to 30 images with out saving and clearing this area.

If a false colour image is required with the DP30 camera select Image / Edit fluorescence color and manually add the false colour. Highlight the image to be saves as before and save as a JPEG.