Supplementary materials
Genome-wide transcriptional analysis of Saccharomyces cerevisiae during industrial bioethanol fermentation
Fig. S1. Real-time RT-PCR validation. This microarray platform was validated by comparing the microarray data with real time PCR results under four conditions, C0 (control), C1, C2, C3. (a) Microarray results of selected genes. (b) Real-time RT-PCR results of selected genes. All data showed here are fold changes for each gene under different conditions normalized by the expression under control condition.
Fig. S2. Transcriptional responses of all genes at the late main fermentation stage in continuous and fed-batch fermentation. Each point represents the log2 change of the mRNA level for a single gene at 36 h relative to 5 h under fed-batch fermentation on the vertical axis and the log2 change of the mRNA level for the gene in the fifth fermentor relative to the first fermentor under continuous fermentation on the horizontal axis.
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Table S1. Transcriptional responses of glucose repressible genes in continuous and fed-batch fermentation.
Genes / Continuous a / Fed-batch (h)b3rd / 5th / 24 / 36
GDH3 / 2.2 / 1.8 / 2.5 / 2.6
ACH1 / 1.2 / 1.2 / 1.3 / 1.6
GLK1 / 0.1 / 1.4 / 0.9 / 1.3
CIT2 / 0.2 / 1.5 / 1.8 / 2.7
HXT7,HXT6 / 4.4 / 6.0 / 1.2 / 1.9
GLC3 / 1.0 / 2.8 / 1.0 / 1.5
HXT13 / 2.2 / 3.5 / 1.1 / 2.4
HXT10 / 2.8 / 3.7 / 1.6 / 1.7
PFK26 / 2.8 / 4.0 / 1.9 / 2.5
GPX1 / 3.2 / 5.8 / 2.8 / 2.7
HAP4 / 1.5 / 4.9 / 2.6 / 3.9
GSY2 / 2.2 / 3.6 / 1.7 / 1.9
NDI1 / 1.2 / 0.8 / 0.3 / 0.9
PGM2 / 1.5 / 3.8 / 2.1 / 2.8
HXT14 / 2.3 / 3.9 / 1.2 / 0.8
HXT17 / 1.6 / 2.3 / 1.2 / 2.2
a data represent the log2(change fold) in samples from the 3rd and 5th fermentor based samples from the 1st fermentor.
b data represent the log2(change fold) in samples from 24 h, and 36 h based samples from 5 h.
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Fig. S1. (Li and Yuan, Genome-wide transcriptional analysis…)
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Fig. S2. (Li and Yuan, Genome-wide transcriptional analysis…)
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