Supplemental Table 1.Characteristics of the 261 Microsatellite Markers Used in This Study

Supplemental Table 1.Characteristics of the 261 microsatellite markers used in this study

Marker / CFAa / Mbb / Forward/Reverse primer sequence (5’ – 3’) / Alleles (n) / Product size (bp) / ATc (°C) / PICd / HETe
FH3325 / 1 / 5.4 / F: GGGTAGATTTCAACAAATAGTACTGG
R: AACTGTTTGATTTTGATGAGGC / 8 / 372-400 / 60 / 65.4 / 70.6
FH3603 / 1 / 14.8 / F: TAGAAAGGCCTGGATGTCG
R: CTTTGACCTACATATAAAACAAGC / 14 / 317-397 / 58 / 71.4 / 74.5
FH2313 / 1 / 19.7 / F: AGTAGAAGAGGCCACGCAAA
R: CACGAAGAAAGCCATGGTTT / 8 / 251-303 / 58 / 62.3 / 67.1
REN189I14 / 1 / 21.7 / F: TTTTCCAAAATGAAAACCTGC
R: CAACAATTCCTTTGTTGGTGC / 2 / 227-235 / 54 / 7.4 / 8.1
REN280C08 / 1 / 23.3 / F: GTGTGTATGGGAGCGGAGTT
R: GCAGCCACCTAATTCGAAAC / 1 / 294 / 60 / 0.0 / 0.0
FH3219 / 1 / 23.5 / F: AGATTGTTTATGCAGGCATTG
R: CTGCCAGCTTCCTACAGC / 7 / 296-332 / 58 / 55.0 / 62.1
FH3413 / 1 / 24.6 / F: AGAGTTGAAAGGTTGAAAATGG
R: TGTGGTCACAAGACTTTAGCC / 8 / 354-390 / 60 / 73.4 / 77.4
REN136G19 / 1 / 24.8 / F: GCTCAGAGGCAGATAGAAA
R: CTGCACCAAAGAAACTCC / 2 / 337-345 / 54 / 34.0 / 43.2
REN307D02 / 1 / 25.0 / F: CAGACATAAGATGCTCGCCC
R: CCCTTGGGAACTTGAAAACA / 1 / 218 / 58 / 0.0 / 0.0
C01.673 / 1 / 26.0 / F: CCACCACTTACATAAACATGGC
R: TTCCAGACTGCTGCCTCC / 3 / 162-166 / 58 / 39.0 / 43.7
REN135K06 / 1 / 30.2 / F: AATTGATTCATGACCCACTAA
R: GGACCTATTCTGAAGCCTAAC / 3 / 152-156 / 56 / 49.4 / 54.9
FH3922 / 1 / 35.2 / F: TTTTGTCAAGCAGCCCTATATC
R: TGGCCTTTAATTTATCATGGAG / 11 / 490-574 / 60 / 82.2 / 85.1
FH3300 / 1 / 39.8 / F: TGAAATGGTACTTCAGCATCG
R: ATCAGGCCCAGATAGTATGC / 7 / 444-468 / 60 / 69.4 / 74.6
C01.424 / 1 / 58.7 / F: AGCTTAGCTTACTGCCCTGG
R: TCCTTTGGTTTTTAGCAGGG / 4 / 179-191 / 58 / 55.7 / 63.3
FH3894 / 1 / 79.7 / F: TTGCCACAAATCACTTAAAGG
R: GCAAAGGTGTATTTACATGATGG / 10 / 204-248 / 58 / 83.7 / 85.0
FH3652 / 1 / 82.3 / F: TGATTGAACACAAAATGAGAGC
R: GACACCAAATGTCTGTTTCTGC / 4 / 213-229 / 60 / 39.9 / 45.9
REN192P12 / 1 / 83.3 / F: TGCTGTATGTCAACAATACTTCAAT
R: GCACTTTAGAAGAAAGCCTGG / 2 / 154-156 / 62 / 10.2 / 10.4
FH2309 / 1 / 85.3 / F: GACTGAGTTCTTTCAGCACAGTG
R: GGCAGCCTTATTATTCATGGA / 8 / 355-415 / 60 / 67.3 / 71.0
REN47D17 / 1 / 85.5 / F: GGCACTTGAGCTCTAATCCTA
R: TGCTAATGAATCCACAGAATG / 4 / 334-358 / 58 / 48.7 / 50.8
FH3883 / 1 / 95.9 / F: GGTGAATCAAGAAAGCATGG
R: TTCCAGGCTAAGGAAAATAGC / 2 / 254-268 / 58 / 37.0 / 51.3
REN04I24 / 1 / 105.0 / F: TATCTTGGCTCCATTTCAG
R: AATCACGGCCTCACGAACTC / 2 / 337-345 / 54 / 5.6 / 5.9
REN06N11 / 1 / 105.5 / F: AAAATAACACAGCATCAGG
R: ACACAGGCACAGGAGCATC / 4 / 325-339 / 52 / 39.5 / 53.6
FH2326 / 1 / 108.7 / F: GAATCCCCAATGTACATGGC
R: CAGCCATCCAGGAAATCG / 7 / 255-279 / 56 / 72.1 / 78.6
FH3322 / 1 / 109.9 / F: AACAGGCGGATTCTGTAGC
R: CTGAACTGGTCCCTCAAGC / 6 / 345-369 / 58 / 50.4 / 58.6
FH3505 / 1 / 110.1 / F: ACTGGCCAAAGAGTACAAGG
R: AGTCATGCTGATTTCTGTGC / 7 / 193-237 / 56 / 64.4 / 72.0
FH2598 / 1 / 111.3 / F: CCTTGACTCAGCAGCCCTAC
R: CGACCTATCCATACATGCCC / 9 / 317-389 / 62 / 71.0 / 76.6
FH2634 / 1 / 112.9 / F: AAAGATTGTCTTGACACGCTG
R: GAAGGAAGGAAGGAAGAAAAGA / 9 / 335-371 / 60 / 66.1 / 76.8
REN223C18 / 1 / 114.3 / F: TGTGTCCAGCATGTGTCTGA
R: GATGGGTGTGAGGGAGAGAG / 2 / 295-301 / 60 / 29.1 / 41.2
FH2294 / 1 / 115.1 / F: GGGTCCTGGGATCAAGCC
R: TTAGTAAAGAGACAGCTGATTGCC / 7 / 213-237 / 58 / 64.3 / 72.8
REN229P15 / 1 / 117.0 / F: ACTGACTGATGTCCTGTGCG
R: ATCTACCCTGCAACCCTGTG / 4 / 308-314 / 62 / 54.1 / 65.8
REN143K19 / 1 / 121.0 / F: GTCAGAGCCCCAGAGACAAG
R: CTTTCTTCCGCCACTACCTG / 1 / 135 / 60 / 0.0 / 0.0

Supplemental Table 1. continued

Marker / CFAa / Mbb / Forward/Reverse primer sequence (5’ – 3’) / Alleles (n) / Product size (bp) / ATc (°C) / PICd / HETe
FH3210 / 2 / 16.7 / F: CAAATGCTTTAGATCAGACATACG
R: CTGAAAAATCTGTAGCGATGC / 12 / 319-379 / 61 / 83.1 / 85.4
FH2613 / 2 / 41.9 / F: AACAATGAAAAGGAATGCCA
R: TAATAGCTGCTTTGAAGCCTTC / 9 / 143-223 / 58 / 30.3 / 34.2
FH2132 / 2 / 73.7 / F: CACTGGGAGTGGAGACTGCT
R: TGCACAGCCAACTAGAGGTG / 10 / 254-342 / 61 / 62.2 / 67.6
C02.342 / 2 / 84.7 / F: TCTTTGAAATGAAATGGGCC
R: GCTCTCATCCCTGTGAAAGC / 4 / 182-190 / 57 / 60.3 / 83.2
FH3396 / 3 / 4.5 / F: GGGTGTGGAGCCTGTTTAAG
R: AGTCCTATGCACTGAGTTCC / 10 / 380-484 / 60 / 53.0 / 61.7
FH3252 / 3 / 37.2 / F: AGCCTGGAGCCTCCTAAAG
R: TGACATGTGTCCACCATTAGTATC / 5 / 442-498 / 60 / 50.9 / 61.4
FH2541 / 3 / 41.6 / F: CGTATGAGTTGGTATAATCTCAGG
R: TGCTTTTCACCTCCCTCTTG / 5 / 152-176 / 60 / 59.2 / 66.4
FH2984 / 3 / 44.6 / F: AGCCTCTATATCAAGGGATGC
R: AGCTGAGCCTTATTCCCAAC / 5 / 200-216 / 58 / 47.8 / 50.9
FH2320 / 3 / 44.8 / F: TTAGTTAGGGTTTTGGTTTGGG
R: AGCCACTGCAATAAGGCAAG / 6 / 225-245 / 60 / 44.8 / 47.9
FH2145 / 3 / 46.3 / F: CAGTCGGGTTTAGGGTCTCA
R: CAGAGCCCCTGACTGCAT / 4 / 272-284 / 58 / 54.2 / 60.1
REN296H17 / 3 / 49.9 / F: TAATGCTTCTTCCACCAGGG
R: CCCAACAGATCTCTGCTTCC / 2 / 320-324 / 60 / 29.9 / 31.2
REN157C08 / 3 / 54.2 / F: GCAAATGGCAAGATTTCGTT
R: CAAGGGTTAGCACCTGGGTA / 3 / 229-235 / 60 / 27.7 / 35.3
FH3464 / 3 / 56.7 / F: AGCAAAGAATCCAGTCAAGC
R: AGCTCACAGTCCATTTTATGC / 4 / 272-284 / 58 / 4.9 / 5.1
REN273H17 / 3 / 57.1 / F: AGTCCATGGAAGGAGGGTCT
R: TCTGCAGCACTTACCCTGTG / 1 / 182 / 60 / 0.0 / 0.0
REN260I04 / 3 / 66.8 / F: CTGTCAGATGCTCTGTCCCA
R: CCACACAAAGACACACACCC / 1 / 185 / 60 / 0.0 / 0.0
CPH19 / 3 / 68.5 / F: AGTCCTATTGTGAAATTCAGCC
R: CGTATTTTAGGCAATGGCAC / 1 / 158 / 58 / 0.0 / 0.0
FH2858 / 3 / 73.3 / F: AAAGATGGGATGCTTCAGAG
R: GACCCCAAGTTTCAGCTCTC / 4 / 191-199 / 58 / 3.9 / 4.0
REN216N05 / 3 / 76.6 / F: GCCACACACATACACCCAGA
R: CTGCCTCTCTTTCTGTCTCTCA / 3 / 265-271 / 60 / 17.3 / 18.8
FH3377 / 3 / 79.3 / F: GAGGAATTAAGAAGATACGAGACC
R: TTGGAATACGTTTATTTTCAGC / 7 / 195-263 / 58 / 42.9 / 46.5
REN249K02 / 3 / 79.4 / F: TGTGCTTGTAAAGGATTTGCT
R: CCTTCATTAAGGCATCGGTT / 2 / 187-191 / 58 / 33.4 / 42.5
FH4076 / 3 / 84.0 / F: TAAGTTCAAGGCCTGATCCC
R: ATATCACTATGGGTCAACCTGC / 6 / 473-493 / 60 / 37.3 / 40.3
FH3373 / 3 / 85.0 / F: TTCTATTTCATGGCAATCAGG
R: GGTGGAAGAAAACATCCTAAGC / 5 / 354-370 / 58 / 41.0 / 46.5
FH3259 / 3 / 89.5 / F: GTAGGGGGTACCTGGGTAAG
R: CAGCTTGTCATTGTCCTAAATG / 6 / 351-391 / 62 / 23.3 / 24.2
REN73P04 / 3 / 90.5 / F: CGCCCAGGAGCCCCAGTAT
R: CCAAGACACGGACACAACCT / 2 / 218-222 / 62 / 25.6 / 34.4
FH2302 / 3 / 91.7 / F: AGTATTTTCACAATTGCTCAGTGC
R: TTTTGGCCTATCTTAAGAGGTCC / 11 / 171-331 / 58 / 68.2 / 75.0
REN298N18 / 4 / 4.7 / F: AAAACATTTCCCTCCCATCC
R: ACACCCAAAGCTGCAGAGAT / 2 / 183-189 / 60 / 22.8 / 24.4
REN303C04 / 4 / 7.0 / F: GAGAGGTCATTGAGCAAGGC
R: ACTGCTCTGGCTGTCGTTTT / 1 / 380 / 58 / 0.0 / 0.0
FH2773 / 4 / 9.0 / F: AAGAGCATCCAGAAATGCAC
R: ATTCGACCAGTCAGAACCTG / 4 / 202-210 / 58 / 26.4 / 28.3
FH2732 / 4 / 12.7 / F: GAAAGGGTGTGGATAAAGAGC
R: TGGCATATTCAACAAATTGC / 1 / 217 / 54 / 0.0 / 0.0
FH3310 / 4 / 20.2 / F: ACTTCTGTGTTTAAATCAGGGC
R: AGAAAGCACTATTGGGACTGTAG / 6 / 298-326 / 62 / 58.6 / 66.5
FH3106 / 4 / 40.7 / F: CAGGATCTGAAGACCACACC
R: AACACCACTAGGAGGGAACC / 3 / 196-200 / 62 / 52.3 / 59.0
FH2412 / 4 / 40.9 / F: GCTGGGGATTTATTCTGACC
R: AAATTAACCAAATGTTTGCAACA / 8 / 162-186 / 58 / 78.0 / 83.0
FH3873 / 4 / 42.8 / F: CACTTCTGCATTGCTTATGG
R: GTCAGCAATCTCTGATCATCC / 5 / 368-396 / 58 / 45.5 / 51.5

Supplemental Table 1. continued

Marker / CFAa / Mbb / Forward/Reverse primer sequence (5’ – 3’) / Alleles (n) / Product size (bp) / ATc (°C) / PICd / HETe
REN282C02 / 4 / 52.5 / F: TGAGATATGCTCCCCAAGAGA
R: CACCTTGCTCAAGGCACAT / 4 / 195-211 / 58 / 24.4 / 25.6
REN160J02 / 4 / 69.5 / F: ACCGTGCACAAGTCAGTCAG
R: AAGCTGAGCCATTCTTTTCC / 6 / 240-252 / 60 / 41.4 / 43.9
REN262N08 / 4 / 87.3 / F: AGGATTTTGTGGTGATTGGG
R: TTGCAGCCTATTGTGGACTTT / 4 / 136-148 / 58 / 44.4 / 59.0
FH2457 / 4 / 88.2 / F: AACCTGGTACTTTGAATTTGCA
R: TTTGGTCAACATACCTCTCTGG / 8 / 340-368 / 58 / 21.3 / 22.0
FH3928 / 5 / 9.3 / F: CATGCTAATCTCTGTGTCATTCC
R: ATTCTCTTTTTCTGCTCCTTCC / 6 / 222-238 / 62 / 54.3 / 60.1
FH3004 / 5 / 14.8 / F: CAAAAATGACCTCTGCTTCC
R: CATAGGAAGCTCCCAGGTG / 9 / 204-316 / 58 / 57.3 / 64.5
DTR05.8 / 5 / 40.3 / F: AGTCATTTGGCCTTACTTCA
R: CAGTCATGTCATGCTGGTAG / 7 / 354-410 / 56 / 52.8 / 61.2
FH3978 / 5 / 54.4 / F: ACCATAGAAGGAATGGTCAGTG
R: TCAGAATCTCTGGGGTCATTAG / 9 / 322-390 / 64 / 17.8 / 18.7
CPH14 / 5 / 89.4 / F: GAAAGACAATCCCTGAAATGC
R: ACCCCATTTATGAGAATCATGT / 2 / 186-202 / 62 / 1.9 / 1.9
FH2576 / 6 / 10.7 / F: CCCCAAAATCTTATCATTTGC
R: CCTGCACTGGGAAAATTAGC / 9 / 295-335 / 60 / 68.0 / 71.3
FH2561 / 6 / 15.2 / F: TGCTCAAGGTTGAATAAATATGC
R: TTTATGGCCTGTGGGCTC / 16 / 298-354 / 57 / 84.7 / 86.6
REN149M14 / 6 / 29.8 / F: AGCATTTTGAATTGGGCACT
R: CTCCACAAGAGCTGATGGGT / 4 / 352-360 / 60 / 47.6 / 57.1
FH3303 / 6 / 69.0 / F: TTGTGGTCCATTTTACATTAGG
R: GCTTACACCACTTGATCATCC / 10 / 263-311 / 60 / 75.0 / 81.4
REN97M11 / 7 / 4.4 / F: TGAGGCTCCGTGGGTATGTG
R: AGGCCAAGGGAATGATGCTC / 3 / 196-200 / 57 / 27.6 / 31.3
FH3972 / 7 / 12.5 / F: ATAAAGCTGGATACAGTTTGGC
R: AGGCTTTTCTAATGAAAGGGAC / 14 / 214-338 / 62 / 70.2 / 75.9
FH3970 / 7 / 46.7 / F: AAGCTTGAGTTTTGATGCTTTC
R: CAGTTGGTAGAAACCAAGGAAG / 15 / 362-422 / 60 / 78.1 / 79.5
FH2581 / 7 / 70.3 / F: ACACTGGGTTGAGTGGTGG
R: CCAGGGGCTAATATCCCTCT / 7 / 310-358 / 58 / 73.4 / 76.6
FH2973 / 7 / 81.1 / F: TCTGGCCCATTTTCTAATTG
R: CCAAAAACCACTGAAATGTG / 6 / 214-242 / 58 / 59.1 / 64.0
FH3241 / 8 / 3.9 / F: AGTTTTAGCCGATCTATTTGG
R: TCAAGATCCTTGTTTGGTAGG / 3 / 310-318 / 58 / 34.7 / 40.5
FH3218 / 8 / 20.8 / F: CACTCAGCATGGAGTTTGC
R: CTCATGTTCACTCATTGAATCC / 10 / 205-317 / 58 / 70.8 / 71.9
FH3425 / 8 / 24.6 / F: TCACTGAATCTTTGATCTCATGG
R: TGTGACTCATACTGACCATAGG / 13 / 274-374 / 62 / 70.0 / 77.7
FH4003 / 8 / 29.0 / F: TGTAAAGAAGATATGATCCGGG
R: CTTTCAATCTCTCTCTTGCTGG / 4 / 351-363 / 62 / 46.8 / 44.7
C08.410 / 8 / 34.0 / F: GAGGAAAACCAAGTGATTTTGG
R: ACCTGCAAGTGACCCTCTCT / 6 / 100-128 / 58 / 65.9 / 71.2
FH3653 / 8 / 41.0 / F: ACCTGCAAGTGACCCTCTCT
R: GTTTTCATGTTTCCCCATAAGAG / 2 / 256-280 / 58 / 0.1 / 0.1
FH3316 / 8 / 48.2 / F: TGACAAGCACAATTCCAAATAC
R: AACTTGTCTGAGCTTCCATTTC / 6 / 344-364 / 60 / 52.2 / 58.6
C08.618 / 8 / 68.4 / F: CAACCCAGGGTGGAAGC
R: TAGCAAGAAAATGTGCCCA / 4 / 194-202 / 56 / 43.9 / 51.5
FH2263 / 9 / 6.3 / F: CATGTAGAGTGATTAGTTGGTCTTT
R: CTGAATATCCTCTGCCCTTC / 13 / 197-245 / 60 / 75.5 / 80.1
REN144L19 / 9 / 14.1 / F: TGTCATCCTGCATCCAATGT
R: CAATTTACTTTTGGGCGTCA / 2 / 216-222 / 56 / 31.5 / 47.1
ZUBECA3 / 9 / 15.4 / F: TCAGGCCTTTGATGATTTCA
R: CAGGGCTGGCATTTATGTAAG / 1 / 200 / 56 / 0.0 / 0.0
G06401 / 9 / 18.0 / F: TGCTTCTCCTCTGTCTGTATCTC
R: CAGGTCCCCCTACACTAAGTG / 2 / 126-134 / 66 / 3.0 / 2.6
REN126A15 / 9 / 20.0 / F: CTACAGGGCAGTTCCACTCTT
R: AAAGGGATTCAGGGAGGTTTG / 2 / 242-254 / 62 / 35.3 / 45.1
FH2722 / 9 / 28.0 / F: CATCAGCCCTCATGTAAAGC
R: ATCTTTTGGCCTTCATCTCC / 3 / 206-214 / 58 / 35.7 / 45.9
REN278L10 / 9 / 37.4 / F: ACTGTGGGGAGAGGACACAC
R: CCTCTAGACAAGAGCCACGG / 6 / 233-253 / 60 / 66.0 / 71.4

Supplemental Table 1. continued

Marker / CFAa / Mbb / Forward/Reverse primer sequence (5’ – 3’) / Alleles (n) / Product size (bp) / ATc (°C) / PICd / HETe
REN177B24 / 9 / 43.3 / F: GAGGAGCAGGTGAGGCATAG
R: GCTGCTCTCGATCAACACAC / 5 / 366-378 / 62 / 60.0 / 70.6
FH2885 / 9 / 49.6 / F: CTTTTAGGGTGCCTTCAACC
R: TGGATTATTAAGGGGAATTTAGC / 4 / 200-212 / 60 / 44.7 / 55.0
REN287G01 / 9 / 52.2 / F: CCCATAAGGACCACAGGTTTT
R: TTCCTAAGGCCCTCCTGATT / 2 / 187-189 / 60 / 36.4 / 47.9
REN73K24 / 9 / 54.6 / F: AATAAAAGCAATCTCAACTC
R: TCGGCACACTACCTGGCAGTC / 4 / 199-207 / 58 / 45.3 / 70.2
FH2537 / 10 / 3.5 / F: AAAAAGTGTAGAGCTTTCTTCAAA
R: ATTGAGACCCAAGACTGTTAGTG / 5 / 144-166 / 62 / 36.0 / 42.8
FH2293 / 10 / 31.6 / F: GAATGCCCTTCACCTTGAAA
R: AGGAAAAGGAGAGATGATGCC / 5 / 231-247 / 58 / 36.3 / 42.5
FH2422 / 10 / 56.3 / F: TTGCCCGTCCTATACTCCTG
R: CCACATGATTTCACTTGTATATGG / 8 / 186-270 / 62 / 73.1 / 76.9
FH3381 / 10 / 69.0 / F: CCCAGAAACTCAACTGATGC
R: AGCTCTTACACGCATTGAGG / 9 / 268-308 / 60 / 76.3 / 77.1
AHT137 / 11 / 9.1 / F: TACAGAGCTCTTAACTGGGTCC
R: CCTTGCAAAGTGTCATTGCT / 5 / 133-149 / 52 / 43.6 / 53.7
FH2096 / 11 / 14.2 / F: CCGTCTAAGAGCCTCCCAG
R: GACAAGGTTTCCTGGTTCCA / 3 / 96-104 / 60 / 49.3 / 61.4
FH2319 / 11 / 35.8 / F: GGAACACTGTATCCTCAGTGTCC
R: TGGGAAGGAAGGAAGTGTTG / 10 / 292-328 / 58 / 79.2 / 84.9
REN147O02 / 11 / 59.7 / F: CTCCTGTTTTCCGTGGTGTT
R: CCCTCCGAGCAAGTAGCATA / 2 / 241-243 / 60 / 35.3 / 46.5
DGN13 / 11 / 71.1 / F: GGAACCCTCAGCTGTTATATGC
R: GATCTAAGGGCTCCAGAACACC / 9 / 297-349 / 66 / 61.2 / 67.5
REN153O12 / 12 / 11.8 / F: CCAACCTTGGGTCACTTTTC
R: GTGTCTTTCCCTGAGCATCC / 4 / 215-221 / 60 / 44.5 / 57.5
FH3711 / 12 / 23.8 / F: CTTGAACCTGTTATCTGATTTCC
R: GGAGGAGTTAAATCTCAAAACC / 5 / 234-270 / 62 / 54.5 / 55.7
FH3591 / 12 / 44.4 / F: AACTGTCACACAATGTTACATCC
R: TCATTACTATGCAAAACTTCAAGG / 10 / 305-381 / 64 / 81.8 / 84.9
FH2347 / 12 / 62.5 / F: TTAGCTCTTCTGATCTGTCCCC
R: TCTCTTTCCCACCCCTCC / 18 / 445-529 / 58 / 84.2 / 84.2
FH3494 / 13 / 5.7 / F: AAGCCTATTTCTCCCTCTGC
R: CATTCTATTTGCTCCTGTGG / 8 / 213-237 / 58 / 78.7 / 81.6
FH3619 / 13 / 22.8 / F: CTGGCAATCACCATTCTACC
R: GGAAAATCTATTTGTAACGATGG / 8 / 242-274 / 60 / 71.8 / 77.8
REN166I13 / 13 / 48.7 / F: TTTGAATGGCTGTGACATGG
R: CCATCCCACAATGGAAAAAG / 2 / 221-229 / 60 / 11.6 / 12.8
C14.866 / 14 / 15.2 / F: TGTCATAATAGTTGGAATGAC
R: TTAGAGCTTACTCATGATATCTG / 4 / 239-249 / 56 / 54.5 / 65.4
FH3725 / 14 / 24.2 / F: GAAAGAACTCACTCAAAACTTCC
R: AAATGTTACTTCAGAAAAGCTGG / 2 / 161-165 / 60 / 37.4 / 54.0
FH2763 / 14 / 47.3 / F: CAGGATCATCTCTCCCACTG
R: CAGAGTCTCCTCTGCCACAC / 7 / 182-196 / 62 / 65.2 / 71.2
PEZ10 / 14 / 58.4 / F: CTTCATTGAAGTATCTATCC
R: CCTGCCTTTGTAAATGTAAG / 14 / 261-361 / 55 / 75.2 / 83.5
FH4012 / 15 / 13.8 / F: TCCCTATCATCAAGGAGTTCAC
R: CATTCCTTCATTTTCACCTCTC / 8 / 415-443 / 62 / 49.8 / 50.6
FH2535 / 15 / 31.1 / F: GTCATTGACAGACTACAAATCTCC
R: ACAGACTTGCAGTATTTTGTCTG / 3 / 117-157 / 58 / 32.9 / 43.0
FH2360 / 15 / 56.5 / F: CATTGATGCTGAATTTGACTTC
R: TTACACACACTATTTTGCTGGTCA / 15 / 282-332 / 58 / 76.6 / 80.4
FH3939 / 15 / 66.7 / F: CTTTGTCACGTTTGTCTTGG
R: CCTTCTCCTCCTATCTCTCAGG / 13 / 183-307 / 58 / 78.0 / 81.7
REN214L11 / 16 / 8.5 / F: AAATTGCAAATCTTGGCCC
R: TCCATGCTAAGACCCCCATA / 4 / 115-234 / 60 / 32.6 / 42.3
FH2670 / 16 / 10.0 / F: GTGGAGTCCTGTGTCTTGATG
R: CTTCTGTTGAAAAATGCAGTCA / 5 / 189-205 / 60 / 63.3 / 68.6
REN199I06 / 16 / 11.1 / F: ACGCAGTGAGAAGAGAAGGC
R: GAGTGTAAAAGGTTAGGGTGTGTG / 3 / 223-227 / 62 / 34.3 / 44.4
REN85N14 / 16 / 21.1 / F: AAGGCAGGAGGAGGAGCAC
R: TATGGAGATGGAGGGCACAC / 4 / 232-242 / 56 / 18.6 / 21.7
FH3290 / 16 / 30.9 / F: CTGAAATACAGACATCCTGTGG
R: CGCGCAAATAAATAAGTAAAACC / 6 / 284-304 / 62 / 66.7 / 74.6

Supplemental Table 1. continued

Marker / CFAa / Mbb / Forward/Reverse primer sequence (5’ – 3’) / Alleles (n) / Product size (bp) / ATc (°C) / PICd / HETe
REN130B10 / 16 / 31.4 / F: GTGTCTCTGCCTCTGTGTA
R: TATTCATTTCGTAACTGGA / 5 / 131-139 / 52 / 52.6 / 61.8
FH2175 / 16 / 33.7 / F: TTCATTGATTTCTCCATTGGC
R: AGGACTCTAAAAACTTGCCTCC / 7 / 247-271 / 58 / 68.7 / 74.2
REN54I19 / 16 / 34.5 / F: CTTCCAGGGATTTCTTTCA
R: GGGCCTACTTTATGTGTCTG / 4 / 159-171 / 52 / 55.9 / 67.5
FH3058 / 16 / 34.7 / F: GCCTTCCATAGATGAATGAGG
R: CCATACATGGTTTTGAGAACG / 3 / 222-230 / 60 / 42.3 / 54.5
FH3096 / 16 / 35.8 / F: TCAGCTCAGGGAGTGATCC
R: ATGAGGAGGTGCAACTATCC / 5 / 199-207 / 60 / 72.6 / 77.6
REN292N24 / 16 / 45.4 / F: GCCCTCAAGCTCAATCTACG
R: TGCAACAGTCAAGACATGGA / 2 / 140-144 / 58 / 33.8 / 42.4
FH2990 / 16 / 55.5 / F: GGTTGGGAATTTCTTACTGG
R: CTTGCTCAGCTGGAGACC / 7 / 341-451 / 60 / 57.4 / 62.8
FH3592 / 16 / 57.4 / F: AGGTGCTGAGCATGTTATCC
R: GGGTCAAAGTGTTGACATGG / 9 / 284-412 / 60 / 69.7 / 76.9
FH3369 / 17 / 1.9 / F: TCCAGTTACCTAATGTTTAGCC
R: TAGGTGACCACATGGTCTGG / 7 / 308-340 / 62 / 46.6 / 61.7
REN294E18 / 17 / 26.5 / F: CACTGCCATTGCTGTCACTT
R: CCACCATAATGTCACCCACA / 4 / 302-310 / 55 / 5.8 / 6.1
FH3995 / 17 / 45.6 / F: AAGGAAATGCTTTCTCAAAGAG
R: GGTCAATATAGTCCAGTGGCAG / 6 / 334-386 / 60 / 19.8 / 22.1
FH2869 / 17 / 66.8 / F: CATTAACAAAATTGCTTCAGTCC
R: CACACCAATAACCCAGAACG / 2 / 200-202 / 60 / 30.4 / 34.1
FH3944 / 18 / 8.1 / F: CTGTTGAGGAACAGAGAGAAGC
R: TGTGTTAGAATGTTGCTTCGAC / 9 / 484-516 / 62 / 60.8 / 68.1
REN54P11 / 18 / 20.9 / F: GGGGGAATTAACAAAGCCTGAG
R: TGCAAATTCTGAGCCCCACTG / 5 / 233-245 / 55 / 41.9 / 53.2
FH3824 / 18 / 47.2 / F: AGGAAAAATACCAAACCAGAAA
R: TTTATCTCTGATTACCTCCTGCC / 13 / 216-316 / 58 / 82.3 / 87.9
REN248C19 / 18 / 51.9 / F: TGACTGTGGCAAGCAAGAAC
R: GGCAAAGAAAGATGGACTGG / 1 / 319 / 60 / 0.0 / 0.0
REN47J11 / 18 / 52.6 / F: TCTCCTCGCGTGTTTCTG
R: GGGGACACTCAGAAGGACG / 5 / 165-179 / 55 / 39.4 / 41.6
REN50L03 / 18 / 54.3 / F: TTCTTGGGTGTGATAATAGTG
R: TGCACCTTTCACCTACATT / 4 / 263-271 / 54 / 40.2 / 45.5
FH2429 / 18 / 61.2 / F: GATCCACTTTGAATTGATTTTTG
R: TTCAGCAAATGGTTCTGGAA / 9 / 171-191 / 54 / 54.7 / 57.4
FH3969 / 19 / 4.4 / F: TCAGGAATAGACTCAAAATGGG
R: CACTATCTCTTTCTGGGGACAG / 5 / 429-473 / 62 / 53.2 / 60.7
REN183C01 / 19 / 8.0 / F: GGTGTTCACTTGGAGGCTTT
R: TGCTTTTTCTAATGCCATGC / 1 / 253 / 58 / 0.0 / 0.0
REN274F18 / 19 / 11.2 / F: TTCTCGTGACCCCTAAAGGA
R: GGCATAAATTGTCTTTGCCC / 3 / 200-204 / 58 / 28.4 / 33.6
FH3313 / 19 / 24.6 / F: TGCACACCCAAAAAGTAAGC
R: CAATCTGAAGCCAATCTCATC / 8 / 385-421 / 58 / 67.0 / 69.4
FH3491 / 19 / 36.3 / F: CAGGTTCCTTGCTCAGTGG
R: ACCCAAAGTTGGCACAAGG / 6 / 260-304 / 58 / 19.7 / 20.6
FH5165 / 19 / 40.4 / F: TGCATCCCCTGGTTTATCTC
R: AAGAATGAGTTCAGGATGACTGG / 8 / 421-489 / 58 / 66.1 / 73.7
FH3830 / 19 / 45.8 / F: TTGTCTATGAAGCCTGGAAGTC
R: AGTTGTGAGATCTAGTCCCGTG / 8 / 107-435 / 64 / 63.8 / 60.0
REN213G21 / 19 / 45.9 / F: AAGAAAATCACCAGCCCAAA
R: CTCCCTTCATCCTTCACCAA / 4 / 253-267 / 58 / 67.6 / 73.7
DTR19.12 / 19 / 48.6 / F: AATACTAAGAGCTGCGTTGC
R: GGATCCTATTGTGCTTCTGT / 8 / 178-222 / 58 / 69.6 / 74.4
FH3868 / 19 / 50.5 / F: AGCCTGCTTAAGATTCTCACC
R: AAAGTGCTTCCACCTCAGC / 4 / 166-182 / 58 / 25.5 / 28.3
PEZ19 / 20 / 4.3 / F: GACTCATGATGTTGTGTATC
R: TTTGCTCAGTGCTAAGTCTC / 3 / 192-200 / 58 / 19.6 / 22.6
REN100J13 / 20 / 25.6 / F: TGATTGACTCTACTTTACACA
R: TATATTAGGCGGTTTTCTTCT / 4 / 164-174 / 49 / 51.6 / 62.1
FH2158 / 20 / 46.9 / F: ATGGCCACATCACCCTAGTC
R: CTCTCTCTGCATCTCTCATGAA / 11 / 262-342 / 58 / 64.9 / 71.5
FH2312 / 21 / 3.1 / F: AAAATAATACTCATCTATATGCTGCC
R: ACAACATAAGAATGTGTGTCATCA / 5 / 326-342 / 63 / 45.2 / 52.5

Supplemental Table 1.continued

Marker / CFAa / Mbb / Forward/Reverse primer sequence (5’ – 3’) / Alleles (n) / Product size (bp) / ATc (°C) / PICd / HETe
FH2441 / 21 / 32.8 / F: TAGTTGTGTGCATGATCTCG
R: TGGAGAAAGTTCCATGTGCA / 4 / 121-133 / 58 / 14.6 / 15.0
REN285A14 / 21 / 34.6 / F: GGCTCCTGTACACCCCACTA
R: CAAGACCGACCACCCTAGAA / 3 / 309-313 / 62 / 2.3 / 2.3
REN118B15 / 21 / 38.0 / F: TAAACTGGCGTAACCTCTC
R: TCCCAACAGCCAGTCCGTATC / 3 / 222-230 / 52 / 42.2 / 49.8
FH3880 / 21 / 43.3 / F: GCAGCCTTAGAAGATAAGAAAAGG
R: CTCTCACTCTGCGTTCTGC / 8 / 224-360 / 60 / 70.8 / 76.5
FH2233 / 21 / 47.8 / F: CCAATGCCAAATTCCTGACT
R: ACCATTTCCTTTCCCAAAGT / 16 / 262-410 / 56 / 83.7 / 85.5
REN49F22 / 22 / 3.9 / F: GGGGCTCTGTTATTAGGTG
R: TCATAAGGCAAAGAAAACC / 3 / 149-157 / 49 / 33.6 / 38.4
REN68D20 / 22 / 5.5 / F: GCCGCTTTTCAGGTGTC
R: TCAAATGGGGAAAGAAAAACT / 4 / 225-277 / 52 / 32.5 / 37.0
REN42F10 / 22 / 7.4 / F: AATGTCTCATCGGAGGAA
R: AGACCGAGCTTTCATCAGA / 4 / 210-216 / 52 / 51.7 / 58.2
FH3355 / 22 / 12.9 / F: TAGAAACAGATTGAGAGGGCAG
R: ATCTTGAACATCTCTCCTTTGC / 10 / 406-446 / 62 / 76.4 / 78.3
FH3411 / 22 / 39.6 / F: CAAATGGAAGTGAAAAGAAAGC
R: ATATGTTCTGGCTGGATCATTC / 17 / 268-332 / 60 / 88.5 / 90.2
FH3853 / 22 / 64.0 / F: ATAGCCAAAAGGTAGAAATAATCC
R: GTAAGAGGGAGCACAAGTGG / 3 / 337-349 / 62 / 35.1 / 39.6
FH3078 / 23 / 12.1 / F: GCCTTCTTTGGAAAAACACC
R: TTCAGGGAATTCTTTCTTGG / 4 / 190-202 / 56 / 40.2 / 47.9
FH2626 / 23 / 19.2 / F: TTCTGCCATTCTGGAAAACA
R: TCCATGGATCAGCAGCAG / 8 / 218-242 / 58 / 49.7 / 50.1
FH2227 / 23 / 38.2 / F: ATCCCAGAACAGAAGAGAGTCC
R: CCTTTGCCTTTGGAATCAAA / 8 / 311-617 / 60 / 69.0 / 76.5
DTR23.28 / 23 / 40.2 / F: CCTGCAAACAACTAAAGAGTC
R: GACACCTCATCAGAAGAACG / 7 / 219-265 / 60 / 65.8 / 72.7
FH3108 / 23 / 49.3 / F: GTCCATGCTTATTTTTTTAAAT
R: ATGATTGGTCTCCCATAGCC / 1 / 232 / 54 / 0.0 / 0.0
REN181K04 / 23 / 55.4 / F: ACAAGCCGACTCTAGCGAAA
R: AGATGGGGCCTAACCAAAGT / 4 / 212-230 / 60 / 45.8 / 56.9
FH3023 / 24 / 7.4 / F: AAGCTCATGGAGTTTCTCACTC
R: AGGGAGCAGCTTTGAGTTTC / 8 / 336-396 / 58 / 48.7 / 52.5
FH2261 / 24 / 21.3 / F: TTAAAACAATGCCTAGACAATGC
R: GCCTCTCCCATTCATGCTAA / 5 / 178-194 / 58 / 56.6 / 66.7
FH3287 / 24 / 44.4 / F: AGGAATGCAGCAAAGTGG
R: AGTCCATGCACACAGAAGG / 14 / 336-480 / 54 / 76.6 / 83.3
FH2324 / 25 / 17.5 / F: AGCTCTATGAAAGGTGATTGCC
R: AGACAGCCATACAAATGAGAATTG / 11 / 251-287 / 64 / 70.7 / 72.5
FH2141 / 25 / 34.8 / F: CAATGCTCCTTAGATTGCAGG
R: ACCCAAGGAAACTAGAAGGAAG / 11 / 530-622 / 58 / 73.6 / 79.7
FH3627 / 25 / 42.2 / F: CCCACTTAAGCAACACAGC
R: GAACCAAGCACCATAGAACC / 7 / 374-398 / 58 / 63.6 / 70.1
DTR26.9 / 26 / 11.7 / F: TAAGCACTAAAGTTTCCCCA
R: GATAAAGACCATTGTGAGCC / 6 / 294-314 / 56 / 53.2 / 58.3
REN299M21 / 26 / 20.5 / F: AAAGTTGCCCACCTGTTGAC
R: GACTTGGAGAGGACTGTGCC / 1 / 242 / 60 / 0.0 / 0.0
REN01O23 / 26 / 23.4 / F: TTCCCTGCAGCCCTTCCTCA
R: TGTGCCTCATTCCTTTTTAT / 2 / 195-203 / 54 / 23.4 / 27.1
DGN10 / 26 / 26.1 / F: TCTGCCTATGTCTCTGCCTCTC
R: GGCACCTATTCTGGGAAACTTT / 7 / 243-267 / 62 / 33.6 / 36.3
FH5173 / 26 / 31.4 / F: TGCTGAAATTTTGGGGTTATC
R: GCCATGGACCAAGCTGAC / 4 / 226-234 / 58 / 49.0 / 59.6
REN88N03 / 26 / 31.6 / F: TGCTTCTTGACTTCCCCTACA
R: GGTTTGCCTCCCTCTATGC / 8 / 149-167 / 60 / 56.8 / 66.5
REN276I22 / 26 / 33.4 / F: AACAATGTCCACAACAGCCA
R: CATTTTTGTGATGGCTGAACA / 4 / 159-165 / 58 / 46.8 / 54.4
FH2130 / 26 / 35.5 / F: GCTGTCCTGCACTTTTCCTC
R: GTTAAGGAATAGTTGGGGGTCC / 7 / 292-312 / 62 / 49.1 / 53.1
C26.733 / 26 / 37.9 / F: CCCTCTACTTATGTCTCGGCC
R: GAGAGGAGAAACAACCAACACC / 2 / 243-255 / 58 / 2.4 / 2.4
FH2289 / 27 / 5.0 / F: CATGGTCTCAGGATCCTAGGA
R: CTAAGCATTCTCTCTGATGGTCTT / 6 / 285-305 / 63 / 55.1 / 62.9

Supplemental Table 1. continued

Marker / CFAa / Mbb / Forward/Reverse primer sequence (5’ – 3’) / Alleles (n) / Product size (bp) / ATc (°C) / PICd / HETe
LEI002 / 27 / 21.7 / F: CCCAATACAGCAAGACCTCT
R:ACCTACTCTGCACAGAGAAGAAAGGAAG / 3 / 128-146 / 55 / 37.8 / 47.8
FH3924 / 27 / 39.3 / F: CAGCCTACAATTCCAACAGC
R: GCTGTGCTCAACATAGTAATTGG / 5 / 353-397 / 60 / 7.3 / 7.6
REN72K15 / 27 / 47.6 / F: CCGATGCCTGTCCTTTGA
R: ACCTGGCCTCTTCTGTTGTCT / 4 / 246-258 / 54 / 43.6 / 52.6
C28.176 / 28 / 4.5 / F: TCAGGCTCTCCAGAGACACA
R: TTGCCAATAGCAGACTGTGG / 4 / 186-222 / 58 / 29.7 / 35.4
FH2585 / 28 / 26.0 / F: TCGATGTCTGCCTTTCTTGA
R: GCCCCCTCACCTCATATTCT / 9 / 288-324 / 58 / 55.3 / 60.1
REN51i12 / 28 / 37.4 / F: ACTTTCTTTGAGCGAGGTG
R: CTGGCCTGTGCTGGGTGAG / 4 / 212-226 / 55 / 63.2 / 71.2
FH2952 / 29 / 5.6 / F: AAAGGCATGATCCTCAGAGA
R: CCAGCTCTTGGTTTCATCAT / 11 / 289-357 / 58 / 70.5 / 75.1
REN52D08 / 29 / 16.8 / F: CAAAAGGAACCAGACATG
R: GTGTGTTTTTCTGCTTCTC / 2 / 157-165 / 52 / 37.3 / 53.3
REN164F23 / 29 / 27.8 / F: GGAGGTGAAAGTGCAATGCT
R: GGGAACAGTGGTTACGAGGA / 3 / 282-296 / 60 / 46.2 / 52.3
REN45F03 / 29 / 28.2 / F: CTGGAAGTTGAGAAGTAGGAG
R: TGTGATGGTATTTAGAGGTGA / 4 / 258-276 / 58 / 55.3 / 60.5
REN169O18 / 29 / 34.7 / F: CACCCAACCTGTCTGTTCCT
R: ACTGTGTGAGCCAATCCCTT / 8 / 155-171 / 60 / 73.7 / 75.1
FH2385 / 29 / 35.3 / F: AGTTCCCTTTATTCTTCCAAAGTG
R: GATGAATCACTAATTCTATTCCCG / 7 / 300-344 / 63 / 48.5 / 52.6
C29.002 / 29 / 36.4 / F: TATTAAATCCCAGTCACCACCC
R: AGGTCCCAGACCGAGTCC / 3 / 212-218 / 60 / 17.7 / 19.1
FH3082 / 29 / 40.2 / F: AGTAAAGGGTTCTCAAGTGTGC
R: CATTTGTCATTGTGGAAAACC / 4 / 218-228 / 58 / 39.3 / 41.7
REN187M05 / 30 / 5.9 / F: GCAGCCACAAATACCAAGTG
R: GTCCCATTCTGCTCCCTCTT / 2 / 332-334 / 62 / 35.7 / 48.6
FH3489 / 30 / 6.1 / F: GGCCACGAATGCTATTTACC
R: TCCTCATATGTCCTCAACTGC / 5 / 254-264 / 60 / 59.8 / 70.8
FH3761 / 30 / 8.1 / F: GCTGAGAGAGTCAGGAAAAGC
R: AATAAGGCTGGTGTGAAAGG / 1 / 203 / 58 / 0.0 / 0.0
REN51C16 / 30 / 11.9 / F: CAGTTCATCCTTCCCCCTCTC
R: GTGCTAGTCTGGCTGTGCTCA / 3 / 258-272 / 58 / 31.0 / 35.3
REN248F14 / 30 / 21.5 / F: CACCTTCGTCTTGTGCAGTT
R: ACTAGGCAGGGTGAGCTGAA / 4 / 212-222 / 60 / 21.5 / 21.1
FH3053 / 30 / 36.7 / F: GATTAAAAGGGCAAGCAACC
R: TCATCTCCAGCTTTTCATGG / 5 / 189-199 / 58 / 52.2 / 60.3
FH2189 / 31 / 51.8 / F: CCATAAGATACTCAGAAACATGCA
R: CACAGTGAGGAGGGGTGG / 6 / 258-278 / 60 / 56.7 / 66.1
REN109B10 / 31 / 13.3 / F: TTACTGAATGGAGATGACTGA
R: AAGAGCTGATTTTGTAGGTG / 3 / 229-237 / 58 / 6.1 / 6.4
FH2712 / 31 / 37.5 / F: AAGGTAGTCCCACGATCCTC
R: GAGCCCTGTTCTCAGGTTG / 5 / 172-184 / 60 / 54.3 / 63.7
REN244E04 / 32 / 7.5 / F: GAGGAAGAGAAGGGAAGCAAA
R: ATGGCCTATGATTGAGCCTG / 2 / 130-138 / 60 / 36.6 / 60.5
CPH2 / 32 / 9.6 / F: TTCTGTTGTTATCGGCACCA
R: TTCTTGAGAACAGTGTCCTTCG / 4 / 95-105 / 57 / 50.5 / 61.1
REN41D20 / 32 / 12.3 / F: TGTCTATGTAATATCACAGG
R: TTCTGGGTATTTATCTGAAG / 4 / 205-217 / 54 / 58.8 / 70.7
FH3744 / 32 / 16.0 / F: GATAAACCGTGTGATAAATTGG
R: TTTCTGTTAATGTAACCCTAGCC / 7 / 510-554 / 60 / 26.0 / 30.0
FH3635 / 32 / 20.0 / F: TGTCAGTCCCTGAACATTTACC
R: AAGCATCTGACGATTTCAGC / 3 / 410-422 / 58 / 11.0 / 11.8
FH3294 / 32 / 29.3 / F: CAACATCTCCCTGTTTCTTCC
R: TCTCACTTACATGTGGAATGG / 10 / 317-349 / 60 / 69.7 / 72.6
MS33.3.2Mb / 33 / 3.2 / F: GCATAGGGGGAAAATCATTC
R: CCTAAAGCTGACCCCTGACT / 3 / 466-486 / 58 / 46.0 / 52.3
FH2790 / 33 / 5.5 / F: CCAATATTGTTAAGAAGTTCAAGC
R: AGGCCTTCTCTGTCCTCTTG / 4 / 204-214 / 62 / 32.6 / 37.9
FH2965 / 33 / 9.1 / F: TGACAATTGATATGGGCAGA
R: TAGTTCATTAGCACAGTGTCCA / 6 / 212-232 / 56 / 59.4 / 62.9
REN112D03 / 33 / 9.5 / F: AAAATCTGGGTAGGGTTATG
R: AAAGAGTGGACTATTCAAAG / 1 / 220 / 58 / 0.0 / 0.0

Supplemental Table 1. continued

Marker / CFAa / Mbb / Forward/Reverse primer sequence (5’ – 3’) / Alleles (n) / Product size (bp) / ATc (°C) / PICd / HETe
REN153J07 / 33 / 12.2 / F: GGCCTGATGAACGAACACTT
R: TCCCCCTTCCTAACAGAACC / 3 / 285-289 / 58 / 26.2 / 25.8
FH3608 / 33 / 12.7 / F: GACCACAAAGAAATCACTTGC
R: GACTTGCGGGATGAAAGG / 4 / 382-394 / 56 / 39.9 / 51.9
FH2361 / 33 / 19.1 / F: GCTTGGAAGGTGAGACTGAATG
R: AGCACTTAGAATGTACCAGGCAC / 15 / 336-432 / 66 / 68.7 / 71.8
REN98D17 / 33 / 24.5 / F: AGGGTGGCCAGAAGGTC
R: GAAGGAAGGTATAGGGGTGTG / 3 / 205-213 / 56 / 37.7 / 59.7
REN291M20 / 33 / 27.2 / F: CCTCTAGATCCATCCATATTGTCA
R: TGTCCACCCACAGATGAATG / 3 / 137-145 / 60 / 52.8 / 62.8
FH2165 / 33 / 32.6 / F: GGATGGAGTCCCACATCG
R: CCTCACCCCAAATTCTTTAGC / 8 / 304-472 / 58 / 73.2 / 76.0
FH3721 / 34 / 4.5 / F: GTTGGCAAATTGAACACCAA
R: ATTACTTTCAACAACCCACTCC / 10 / 197-245 / 60 / 71.9 / 75.5
REN243O23 / 34 / 26.8 / F: TCTGAGGAAAGCCAATGTGA
R: TCATAATGATGCAGCTGGGA / 3 / 258-268 / 60 / 27.5 / 32.4
FH3836 / 34 / 34.9 / F: TAAAATAGGAGTGCTCAGGTGG
R: TCCTCTGTCTTCATTTCATGC / 6 / 444-472 / 61 / 30.8 / 36.4
FH3570 / 35 / 13.0 / F: CATTTGCCGTAAGAAGAAGC
R: TCACATGGAGGAAGAAATACC / 7 / 203-327 / 56 / 68.7 / 72.7
REN214H22 / 35 / 22.5 / F: TGAAAAGAGCTACTGTTTCTATGC
R: CACCTCCTCCATGGGTTTTA / 4 / 319-325 / 60 / 49.6 / 57.2
REN94K23 / 35 / 23.4 / F: ACGTGCTTAAAATCTGTTGAA
R: TATGCTGGAAGGTGGTATTTT / 6 / 234-250 / 52 / 44.6 / 47.4
REN106I07 / 36 / 7.1 / F: TTCCCCAAGCCACACC
R: AACCACTATCCAACTTTAT / 2 / 210-214 / 49 / 35.4 / 44.6
REN179H15 / 36 / 16.7 / F: AGCCATTTGCCAACCTACAC
R: AACACACACCAGTTTTGCCA / 4 / 304-316 / 60 / 42.8 / 50.5
REN252E18 / 36 / 25.5 / F: CAGCATTTCCTCACTTTCCC
R: GGGGAGATTGTGTATCGGAA / 4 / 251-261 / 60 / 24.9 / 28.3
FH2998 / 36 / 28.1 / F: GATTTTGATACCCTGAGAATGC
R: CTCACTGGCTCTCACATGC / 7 / 201-225 / 60 / 59.9 / 66.2
DTR36.3 / 36 / 38.0 / F: TTCCTAACCAGCTACCAGAG
R: TGTTGATATTTGATGTTGCC / 7 / 202-246 / 54 / 77.2 / 79.8
FH3272 / 37 / 5.0 / F: GGATCCGGGATAGAGCAG
R: CTTCCCCAATCTTCCTTGTC / 15 / 295-399 / 60 / 69.6 / 70.4
REN67C18 / 37 / 22.3 / F: TCTGTGCGTTTCCGTTTATG
R: TTAGTACCTGTTTGTTATCC / 5 / 121-133 / 49 / 50.2 / 59.1
FH2532 / 37 / 25.1 / F: CACGCAGAAAGGCAGAAAG
R: TTTCCATAGTGGCTGCATCA / 14 / 332-432 / 58 / 67.3 / 70.5
FH2766 / 38 / 6.3 / F: TTTCAAGAAAGGCAGAAAGTG
R: TTAAGGGAGCCATGAATCTG / 2 / 252-262 / 58 / 1.4 / 1.1
REN164E17 / 38 / 22.6 / F: GGTCTTCACCCATCACCATT
R: TTAGATGGAAAATGTGGCCC / 5 / 137-153 / 61 / 59.4 / 66.1
FH3399 / 38 / 26.0 / F: TCTCTATGCCTGCAGTTTCC
R: TTCTGATGCCCTCATAAAGC / 10 / 224-272 / 58 / 57.3 / 66.3
REN230I20 / X / 12.1 / F: CACTTAGCAGAGCACCCTCA
R: CCAGGTTCACTGCAGCACTA / 2 / 212-218 / 61 / 22.8 / 24.2
REN101G16 / X / 17.8 / F: AGACATGTGAACCTGCATG
R: TATCATCTCACTCTGGCACA / 4 / 141-149 / 52 / 31.7 / 38.0
FH2985 / X / 20.7 / F: AGGGGCAACTCAAAGGTTAC
R: ATGTGTGGAACTGAGCTTGG / 6 / 303-323 / 58 / 39.6 / 43.7
FH2916 / X / 34.0 / F: CTGGCATACCTCAACAAAGC
R: CAAAATTGTGGACCTAAAATCG / 16 / 207-251 / 52 / 85.9 / 87.9
FH1020 / X / 36.6 / F: TCTACAATGTCAGTGGAAAGC
R: CACAGTGGGGAGTAGACAGC / 8 / 155-274 / 60 / 69.1 / 76.2
FH3027 / X / 40.8 / F: GTTTCCTCACATGCAAAAGC
R: GCTGGAGGTCAAGGATAAGG / 6 / 196-213 / 58 / 52.7 / 55.1
REN130F03 / X / 70.1 / F: ATCGAGTCCCACATCAA
R: TTCTACTTCAGCCCCTACC / 3 / 169-181 / 52 / 17.2 / 19.7
FH2584 / X / 103.9 / F: GTTAGGTTCACAGTGGGCGT
R: ACTCAAAGACCTGGAGGGGT / 8 / 300-320 / 58 / 57.7 / 64.8
REN44K10 / X/Y / 3.0 / F: CATATTGGACCTTCACAT
R: TTAACGCACAACTTCATC / 3 / 200-208 / 49 / 47.9 / 58.2

aCanis familiaris autosome; bPosition of the marker on CFA (Mb); cAnnealing temperature;

dPolymorphism information content (%); eMean observed heterozygosity.

Supplemental Table 2.Hip scoring scheme according to FCI as applied in German shepherd dogs

CHD / Rating / Radiological signs
A / No signs of hip dysplasia / The femoral head and the acetabulum are congruent. The craniolateral rim of acetabulum appears sharp and slightly rounded. The joint space is narrow and even. The acetabular angle according to Norberg (adapted for position I) is about 105° (as a reference). In excellent hip joints the craniolateral rim of the acetabulum encircles the femoral head somewhat more in laterocaudal direction.
B / First signs of changes / Either the femoral head and the acetabulum are slightly incongruent and the acetabular angle according to Norberg (adapted for position I) is about 105°; or the centre of the femoral head lies medial to the dorsal rim of the acetabulum and the femoral head and the acetabulum are congruent.
C / Mild hip dysplasia / The femoral head and the acetabulum are incongruent, the acetabular angle according to Norberg is about 100° and/or there is a slightly flattened craniolateral rim of the acetabulum. Possible presence of irregularities or only than slight signs of osteoarthrotic changes of the margo acetabularis cranialis, caudalis or dorsalis or on the femoral head and neck.
D / Moderate hip dysplasia / Obvious incongruency between the femoral head and the acetabulum with subluxation. Acetabular angle according to Norberg more than 90° (only as a reference). Flattening of the craniolateral rim of the acetabulum and/or osteoarthrotic signs.
E / Severe hip dysplasia / Marked dysplastic changes of the hip joints, such as luxation or distinct subluxation. Acetabular angle according to Norberg less than 90°, obvious flattening of the margo acetabularis cranialis, deformation of the femoral head (mushroom shaped, flattening) or other signs of osteoarthrosis.

1