Sequences of Evolutionarily Conserved MED Subunits
Alignments compare the most highly conserved homology blocks from S. cerevisiae (Sc; upper line) and human (Hs; lower line) MED subunits with proteins from C. albicans (Ca), S. pombe (Sp), A. fumigata (Af), N. crassa (Nc), D. discoideum (Dd), A. thaliana (At), C. elegans (Ce), and D. melanogaster (Dm). The primary sequences of putative MED subunits for C. albicans, N. crassa, A. fumigata, and D. dictyostelium were deduced from genomic sequences (details are available upon request to ). We used genomic sequences of N. crassa, A. fumigata, and A. thaliana that are available on the following websites: http://www-genome.wi.mit.edu/annotation/fungi/neurospora/; http://www.tigr.org/tdb/e2k1_/afu1/; and http://www.tigr.org/tdb/e2k1_/ath1/. Preliminary sequence data for C. albicans was obtained from http://www-sequence.stanford.edu/group/candida. Flanking numbers indicate positions (when known) in the complete peptide sequences. Sizes of intercalary regions are shown in parentheses. Solid and shaded backgrounds indicate identical or similar amino acids (a.a.), in at least two of the aligned sequences. Amino acids considered similar were R/K/H; A/S/T; I/L/V/M/C/F/Y/W; G/P; and E/D/Q/N. Positions are indicated with an asterisk if they harbor identical or similar a.a. in all five sequences, or with a plus if in 4 of 5. Predicted helical (H) or extended (E) secondary conformations are indicated above the alignment.
Srb2/Trfp
EEEE EEEEE HHHHHHHHH EEEEEE HHHHHHHHHHH
Sc 1 MGKSAV(26)DPWSIDFRTYR(60)IDTILSSKLSNI(32)FKGLLIELQA(41)CDLAYQYVRAL 208
Ca VRVLlV(23)GKWNFNFKIFR(82)FDNFIGAKLQSL(34)FRGLLIQIEM(44)GDLCLQYSKIL >233
Sp 1 M-ATFL(16)KKwVVQYKLYR(34)LEDIIVRTKLWN(29)WKGILFHVTY(42)LDSKLLLQLFK 170
Af CRVFFi(28)GRwALEHKLMR(91)FKHFvYAMQPFW(34)VRGTVVEIEY(47)ADIDAEYSATA >299
Dd 3 KRiEFL(07)GtINCLYLERV(37)MVSILEKTNSYK(29)IRGILVEVEY(50)LHTSYQYVNLF 176
At 1 MPVKWL(31)GtwKATLNYYK(48)IQMIMESLQSYK(31)IRGVVMEVEY(51)QHTAVGYAFFM 207
Ce 1 MGVTWV(22)GTFIVDVTPFN(47)FDLILSKLSSGL(33)VKGVVVEIEY(43)LDTMWQYLGIA 191
Dm 1 MGVTIL(30)GQFLVDCETFI(49)FDLLMLKMTNTF(32)FKGILIEIEY(57)MDTVKQYLEQF 214
Hs 1 MGVTCV(30)GTFCVDCETYH(47)FDVLMVKLKGFF(31)VPGISVEVEY(42)ADTMVQYMELF 195
+* * + + + * ** *+*++*+*++ *
Srb4/TRAP80
HHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHH EEEEE HHHHHHHHHHHHHHHHHHHHHHHHH
Sc 208 LEHINLAMNESSLALEFVSLLLS(75)HDYWNKIMQSISNKDVIFK(100)IRKQIQLLKKIiFEKELMYQIKKEC 449
Ca 71 SKNINNALNETQLSLDFVSLLIS(75)RRYWNMiNLVLANDEVLFR( 92)IINDIEKARFFLFEEDLFHQLIREA 304
Sp 109 IEQIMIAQTECSLALDMTSLLLS(70)HEYYTELVKVKEASWPLFN( 73)LLKDIYKLQEILFEMDIWNSLLQEA 318
Af 80 LRSYRQAHMEAMFALDFVSMLLS(75)TRYWHEVLAVKDKGWKLCR( 77)IEGRILQARDTLYEEELFHELFREA 298
Nc 110 IMSLGQAIQISSNFLDFISLLLS(76)TKYWQEVLTVSQKGWSTAR( 76)LDDRVKEARNTiFAQELWHEINREG 328
Dd 97 HEHKNMARIELEQLIYLIDMLRN(55)SGwWndVSQLRTK-WRLKG(244)CTNQLNKAQKYQFNYEIFEQLSKEV 461
At 79 VENLQLAHQELTVIIDLIDTVQA(93)ARFYGALIRLQRN-WKvKR(105)HSLLREVHKSIFAEQVLFDMLNREA 342
Ce 154 IPIFSESLNQLNSLLDVISVTKS(71)TKFFEELREMREI-WRVRK( 95)WKDSLKWAQNTLLLRDVFKTNLLAE 385
Dm 86 RNKLKDALTEICVLSDVISIAKD(55)SDFHIELLRLRQN-WRLKK(106)WQKTLEFAQNVLFCKELFAQLAREA 312
Hs 165 RNNLRSALTEMCVLYDVLSIVRD(59)RDFNSELLRLRQH-WKLRK( 92)WQTKLEAAQNVLLCKEIFAQLSREA 381
+ * + ++*+ + * * + + + + + ++ +** + +
Srb5/Med28b
EEEEE HHHHHHHHHHHH EEEEE EEEEEE
Sc 4 QLSLFGSIGDDGYDLLISTLTTISGN(133)PWSLQISDIPAAGN( 26)LMNGLGYVFEFQYLTIGVKF 222
Ca 4 QLSLVSSIPHNKYLQTISTLQALTGL( 84)VWTLQVSDIPVAGK(115)FLSDLGYEVINQYWQKGVRF 261
Sp 3 ELYLLGVVPSRRFEAVVNSLSKTLDG( 48)QWSMYLEGNSEPKR( 21)FVEKMGYEFSHEYIIQGLEY 131
Af 17 ELLLFASVPAHQHHELLQQLAGLTAM( 94)PWKLEFRDIPEAAT( 21)PMNAWGYNFVTEYAVEGDIF 177
At 4 ECVVQGIIETQHVEALEILLQGLCGV( 36)TWTVKHVGGAMRGA( 23)MFYALGYKLDHELLKVGFAF 122
Ce 27 ECVLYGSIFAKNVPDLERRLAGLCDP( 41)YWQLKYIGVPEPDQ( 21)YAKSLGLRMDYEYMTQGKLW 147
Dm 24 EYLLQGSILDSAVEHLMHRLKGLCDN( 38)PFQMRYLGNPEVDL( 20)FLTEMGFRLEFEYIAKGYMF 141
Hs 20 EYLLQGSVLDHSLESLIHRLRGLCDN( 36)PWHLRYLGQPEMGD( 21)FLMEMGFRMDHEFVAKGHLF 136
** * * + + * + * * + ++ *** * **+ * *
Srb6/Surf5
HHHHHHHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
Sc 4 QALYEKLEQTRTILSVKLAELINMTTIAD(23)MMVNNQTMQLIKNVQDLLILTRSIKEKWLLNQI 88
Ca 6 ISLLQKIDSIIETIIVKFTNIFENLQDAN(10)LAMENNCIQIIRLCQDLISISRNLKEIWVLNSI 77
Sp 15 NTLNSSIDNATLTILSRFQDILDIAINEG(10)YQIECHTVSMVRAVEQLLDVSRQIKSYWLTNSL 86
Af 8 KALHNRINANISQLLQRFENIMATATVRS( 9)LDVESTALVCVRAAEDILSLTRTMKEAWLFGKL 78
Nc 3 PDLENRLTA---SLLRTFQSLLHHGASSV(13)MATDILMNGMVQSVEELLSLTRKMRELWVVGPL 74
Dd 23 QFFQKQIDSKIMQLLENYHNIIKISKVND( 8)YEMETRTSNMLNAGEGLLKIIEELKQNLILNDF 92
At 27 KALLQRVDTDITSVVDNFNQIVNVARVSD( 9)YMMEMRASRLVQAADSLLKLVSELKQTAIFSGF 97
Ce 25 DEFKRRLRDNIKSLNDNFFHIIDAAKVNP(19)NEMAVRAQLMVRASDELLKLTADLKEFLILHDF 105
Dm 19 KSYNARLKDDVRSMLENFEEILKLARRES(14)LEMQVRAANMVRAGESLMKLVADLKQYLILNDF 94
Hs 16 QSYNKRLKDDIKSIMDNFTEIIKTAKIED(14)YEMHVRAANIVRAGESLMKLVSDLKQFLILNDF 91
* * * * ** + + ** * ** * **+ +*+ *
Srb7
HHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHH
Sc 1 MTDRLTQLQICLDQMTEQFCATLNYIDK(32)ELSTDIILKTRQINKLIDSLP 81
Ca 1 MADRLTQLQICLDQLIQQFNSTINYVNT(72)ELCTDLILKSRQIKMLIDSLP 121
Sp 1 MACRCTQLQDTIDEVATQFYSSIHYLSS(31)DLAKDLVTKFMQIDTLINQLP 80
Af 1 MADILTQLQTCLDQLATQFYATIGYLST(95)ELARDLIIKEQQIEYLISVLP 144
Nc 1 MSDRLSQLQEAVDQLMEQFIATYFYIDR(37)ELARDLITREQQIEYLISSLP 86
Dd 1 M-DLITQLQDKLDHLLYVFGTCIGVLQR(85)KMALQVIETSKVIESYIEKLP 133
At 1 M-DIISQLQEQVNTIAAITFNAFGTLQR(32)QLSAGLVKAAKQFDALVAALP 80
Ce 1 MADRMTQLQDMINEMAGLMTNAIGVLQA(21)IFAAGIAKAAKNIEILIDSFP 70
Dm 1 MADRLTQLQDTVNQQAEHFCNAIGVIQQ(29)LFAQLIARCAKDIDTLIESLP 78
Hs 1 MADRLTQLQDAVNSLADQFCNAIGVLQQ(28)LFAALIARTAKDIDVLIDSLP 77
* + ***** ** + + +* +* *+ * +*+ *+ **
Srb8/TRAP230
HHHHHHHHHH HHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHH
Sc 129 WLHELSSSHTSLVKIGKFIPHGLKRRQVIEQCYLKFIPLKRAIWLIKC( 65)ISYFLKLLGNCYSLRLINKEIFHHW(300)
Ca 200 WLNELSNPNVSMTKLGEKIPHGIRNKVLIDCICTKKIPINRSIWFTKC( 90)LSYLLNYIQSLYVESLLDKSLFLSL(353)
Sp 137 WLQGLSDPMVPLSSLIKTIPHGLWGEDILRMLVKFRVPFTRAIWFIRC( 53)YTYLLKLFGRLLAEELVSPKHFLLR(220)
Af 88 WLRDLANPNVPLRKLSRTIPHGIRGRVLLDQCLTKWVPVGRAVWLAKC( 53)VTYAVNLTSRLFFEQLLDHDQYLEW(222)
Nc WLKDLANSAIPLRKLSRSIPHGVRNKVLLDQCLNKNVPIDRAVWLAKC( 54)VNYAIQLAAHLYAEGLMDREHYLDW(227)
Dd 124 WVLKLA-GNEPLQKLASSVPHGYKGETLLKMFLDNQVPLIRATWYTKI( 62)ILYIERLIKWNFSEGLLNKDILLSM 257
At 132 WIESLSQQHKRLRSLADNIP-GYRRKTLFEVLIRNNVPLLRATWFIKV( 51)MNFCHGIAHFLLSKLEIGHHRCFIQ(391)
Ce 113 FFSALARG-TPLQQLSQRIPGFRRKELLFLEFSDYNISYDRAIWCVKL( 56)WNYLTGFMKYMFDDGVLDKQEFLND(793)
Dm 112 WFKDLA-GNKPLLSLAKRAPSFNKKEEIFITLCENQVNMQRATWFIKL(175)WKYCHQLSKYMYEESLLDRQEFLNW(343)
Hs 151 WFTDLA-GTKPLTQLAKKVPIFSKKEEVFGYLAKYTVPVMRAAWLIKM( 72)WDYTEKLAMFMFQDGMLDRHEFLTW(340)
** ** * * +* + ** * * * ** ** ** * * * +* +* *+
HHHHHHHHHHHHHHHHHHHHHHHHHH
Sc LYRLLNILITYGIIKVPTYIRKLISSGLL 591
Ca LYVLINELYQLKLITIASYLRKLIASGIF >734
Sp AAQLLDHLAACGYFFFSKYIARLASLGYL 507
Af VYHIVSELVRSQTFSVGKYLQWLMAKGVA 464
Nc LYHLVSELVRSGIFLVSQYIQWLITRGGL >475
At LQLLVFELIRSGIFNPIAYVRQLIVSGMI 670
Ce TVAIMIEMMREKIFSITDILRELEKDSDL 1062
Dm LVQLFSALIRHDVFSHNAYMHTLISRGDL 730
Hs LVLLFCELIRHDVFSHNMYTCTLISRGDL 659
** *+ +* *+ * + +
Srb9/TRAP240
EEEEE EEEEEE HHHHHHHHHHHHHHHHHHH
Sc 912 KQPYVFVKKHHELIKVKHDSQPFIKFLNFRPPNGIKNFKSLLLSS---SFKEDCLSFAPTLSQTYINQELG(234)
Ca 1047 PMVKTRILKNGNIVNLNNIGLKFWKYLNFSPYKKQKDFQILLIAETHRNTASYATEFLDQIIQNYKECNFG(234)
Sp 740 QPPRVLLKYNEKKLSLDSSAPENWISLCLQPYGESKDFEVFLLSSKSPDVSSKAISFFYDVQLAYENCKLG(271)
Af 1049 APPHLRIRRGKDYLEVLPPAISFWETFGLEPAYGSKDIYAYCIHP--QTAAEAANDFLERLGLLYSSCNLG(242)
Nc 914 PTPHIEIRRYETPFSMLPTAISFWESLGLSPAKGAKDIVSVCLFPNVDGLRDSASAFLQRIRSVYESLKLG(239)
At 539 PSPAILVGYQDDWLKISTNSLTHWEKAPFEPYALPKSINYAVVCPDIDPLTCAATDFFQQLGTVYETCRLG(437)
Dd 1878 PIPKIIVGYKEDWLEVPISTIKFWDKAQLEPYSPKKNITYFVLCPDTTAMRMHVPAFFSDLSCIYETNHLG(433)
Ce 1860 PVPAIMRALTKEAIRAAPNIEQYYEQAGLGPYDQPKDVMYITVIPDDDDIYSRTVEFMDTLTRTYEKMRLG(652)
Dm 1847 PIPSVVVGYEKDWISVAPHSIHYWDKFLLEPYSYARDVVYVVVCPDNEHVVNCTRSYFRELSSTYEMCKLG(390)
Hs 1332 PIPTFLLGYDYDYLVLSPFALPYWERLMLEPYGSQRDIAYVVLCPENEALLNGAKSFFRDLTAIYESCRLG(455)
+ + + * + * * * *+* ++* *+ * * **
EEEEE HHHHHHHHH HHHHHHHHHHHHHHHHHHH HHHHHHHHHHHH
Sc CLILTRLNGILPDDELMNWRRLS(109)DSTQLLETILEEFRNLAALNV(11)IPWHILAVKKMMNTLV 1393
Ca FLVLTRINSIIPDVELVHWKRLS(187)NLDVLMKLIMLQYKQMIALND(11)VPWHINAVGKLLDYLV 165858
Sp WIVIVMKIGEMSDAEYLFWDQAI(101)DPYLMLNLILKHYLSMIYLQF( 8)LPLHISTVLYQKQLLQ 1211
Af WKIIIANTEPVDQDEVDTWTSLV(151)LYENLLREIIARYRDLATLAR(10)FPWHIATALRAQELLS 1580
Nc WRVVVTKCSPMEQQEIEYWASLQ(173)VYEQLLREMLSHFRGLGTLAR(10)RPWHVAVAEKAVRALY 1467
At RDFVITRIGNFFELEYQEWQKAI(267)ESHLILESIAAELHALSWMTV(10)LPFHCDMVLRLRRLLH 1383
Dd CQVVIGKFGIISMQEYNDWKTTI(186)NHQELIRSITRSYHRLSYLNI(10)LPIHFSISKRMSRTVC 2637
Ce WRLVVGRLGRIGHGEFRAWTHLL(228)QTEEVLRHVLESYNALSWLNL(10)LPIHIQHLLRLYHSVA 2878
Dm WRLVIGRIGRIGHSELKSWSFLL(238)LTADVLRFVLEGYNALSWLAL(10)LPINVQTLMDLYYLTA 2615
Hs WRVVIGRLGRIGHGELKDWSCLL(244)QTSDVLRFVLEQYNALSWLTC(10)LPIHFVVLNQLYNFIM 2171
+ ** * ** * ** * * * * +**+*
Gal11/Sur2
HHHHHHHHHHH HHHHHHHHHHH EEEEE
Sc 182 LLQRIPNIPPNINTWQQVTALAQQKLLTPQDMEAAKEVYKIHQQLL(650)FKDSPMDLFMSTLGDCLG 893
Ca 235 LLNKMPKLPAGVNTWNQIFDLIRKRLIPPDMVPTIRDIHGMHLQLF(717)INIDPEKFFYASLANLLD 1015
Sp 359 LFDQIPNLPPNVKIWRDVLELGQSQRLPPEQLKLIGMLYRKHLQII(449)SVVNTPDVIMSSVDEIPS 86
At 1086 AARLLPVIPLVLYRLIENNAM-----QADNLLLAHSHFLAYHPLRF(379)QIADLIDFLHHIIHYEGQ 1524
Ce 1010 IERLLPIMDQYLHVALEQQGY----KLNPQILQSVSMLYKFHAMPI(280)YEQHLCDLFYNCKYFFAG 1347
Dm 953 CLRFLPVLDVVVHRFIELTIT-NVHQILGFILDHLSILYKFHDRPI(284)YMDQICDLFYHIKYIHVG 1299
Hs 960 CLRFLPVFDIVIHRFLELLPV---SKSLETLLDHLGGLYHFHDRPV(289)YMDPICDFLYHMKYMFTG 1307
+ ** * * + * + * *+ +* * +**+
Rgr1/TRAP170
HHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH EEEEE
Sc 93 ALVIRNLTVFTMKELAQYM(11)NSAKKIRFLQLIIFLRTQFLKLYVLVKWTRTI( 6)VLIDLLNWF(20)MTNAKLP
Ca 39 SNIIKYYTQEAYKQLTTAI(11)DIKRKKYFLNVIINLRQDFIKVYTLIKWASIS( 4)KFIDLLNWF(19)YNAAKLP
Sp 20 VETFSHHVLQELVSLAEVL( 4)NVEKKKKILDWLLRSRAFTMRLLVLARWVHLS( 4)RCIDVVAFL(20)LSFARLR
Af 86 VQQCWNELSDLITELAAIH(24)NVQKKLRILDFAHAKRAEFIKLLVLSQWSRRA( 4)KLIDLQNFI(20)LVQAQVA
Nc 53 SQWTHTKLQDEIAYLASKP(23)SLEKKVHLLNFLQDMHSKWVKTLVITEWSRKA( 4)ALIDIRVHL(20)LHWAKVP
At 15 VGRAAEESFLSFKELVDKS( 6)DTEFKVSLLKYVAKTQQRMLRLNALAKWCKQV( 0)PLINYFQDL(24)LQQARAP
Ce 26 LDFAIQHVYHEITVLAELM( 8)EQERKMSLVHFAHATRSQFLKLVALVKWIRIS(23)QSIDYLLDL(19)LELARLP
Dm 41 IDFIIQRTYHELTVLAELL( 4)DMERKVEIYNYAARTRHLFTRLNALVKWGNSV( 7)QIMSFLDKQ(18)LVRARLP
Hs 55 IEFLLHRAYSELMVLTDLL( 4)DVERKIEIVQFASRTRQLFVRKLALVKWANNA( 7)MISSFLDQQ(18)LVHARLP
* *+ + ** ** * + ++*+ * * ++ * * *++
EEEEE HHHHHHHHHHHHHHHHHH EEEEE EEEEEE EEEE
Sc NVDLVTALEVLSLGR(29)LILQRLKDLNLTVSIKIA(3)IPKPLN(2)YHIKNGRIYFTVPNEFEIQLST( 4)SPLFFV
Ca NSDIITALEVLYHGR(17)KILETLNDLNLVLMTRFA(4)IPKRFD(0)YEIKDGRAYIRVSNEFEVSITV(14)SPFYFI
Sp NSDLVTALDILSTGT(23)QILQTLHALNMLIRIRLS(4)IPTPFQ(1)FTIANGRCTFTVPNEFSVSLTT(11)FQWIVV
Af NPDLQTALEILSKGR(18)GALRRLHKINRIISARLA(4)IPHPFR(1)YRVHDGRVTFVVRGEFELDLSI( 6)SQFFFV
Nc GPDLKTALEVLTTGQ(18)EKEAFIDNINTMLSVRLT(6)LPAAFR(1)YNIANGRVTFKVKGEFEVDLTI( 6)QQFWFI
At VYDVPSAVEILLTGS(20)QQKPALRKLEVLVRSKLL(3)LPKEIT(1)VKISKGTVTLSVDGEFKVLVTL( 6)SMWRIL
Ce EYHIAPAIDVLVLGT(21)EQKLVTSRLNQLIESRLS(2)IPPNIK(1)IHINNGLATLLVPGEFEIKITL( 6)TKWTLL
Dm NFHIPAAVEVLTTGT(21)EKRQTLLRLNQVIQHRLV(4)LPQMRE(0)FRIRNGRVTFEVKHEFSVSLTV( 6)VPWRLL
Hs SFAIPYAIDVLTTGS(21)EKQATLHQLNQILRHRLV(3)LPPQLA(1)LTVANGRVKFRVEGEFEATLTV( 6)VPWRLL
* ***** * + ** +* ** ** + * * +* * * ** + ** * **
EEEEEE HHHHHHHHHHHHHHHHHHHHHHHHH EEEEEE
Sc DLKLLFN(58)LYNFLHKYVLTLQLYMVHREFLKLA(18)STITVRYW 416
Ca DFKFLFG(42)LYELLHKYSNSFKIYLLAKQFQTLL(17)SLIVINYW 340
Sp DFQFHLP(34)IYNILHKFCLYQRLNLLSQQTFQLS(16)PRLRLYYW 310
Af DIRFLYS(29)CFNFLHGLVLTNKIHILFKQAIELA(14)RTLVIQYW 378
Nc DIRFLFT(29)CYNYLHEFVLTQKIIEFRRQATELA(14)RAMSIQYW 347
At HLDLLVG(33)LYAVLHELCVAIVMDTVIRQVRALL(34)PGMKLFYW 315
Ce NIKILVE(36)AFSFLHSFCVSLQLDVLFCQTSRLA(17)RVLVVGYW 335
Dm DIDVLVE(36)VYNCLHYFCQSLQLEVLYTQTLRLN(17)VKLTVSYW 327
Hs KLEILVE(36)MYNCLHSFCLSLQLEVLHSQTLMLI(16)KCLSLSVW 340
* *+* +* *** *+ + * * * * * * **
Rox3
EEEEE HHHHHHHHH HHHHHHHHHHHH
Sc 33 DLISVYGLDDISRQVARTN-LDGTKAVKLRKTYKNQIADL 7
Ca NLIKLYGLIPVTKSLARTN-PDGSKGVKLRKSYKNHIQDL >58
Sp 25 NLIELYGLTELAKKVGRVD-EFGNK-RKMRRSYKAYIQDL 62
Af 16 DLISLYGLGPVAKSVARMDPVTGEKINRLRKSYEGKLKGL 52
Nc DLFEMYGLADLAAEYARIK--DGQK-NALRKTYKGHIKKL >58
*** **** **+ *+* + * * +*****+ * *
Sin4/TRAP95
EEEEE EEEEEE EEEEE EEEEEEEEEE EEEEE EEEEE
Sc 9 SWSKTGIIAYS(170)ILEFRWL(41)ACLAIRKNGQI(58)ISFYKLHVNW(133)FVSFYFEDGTIESYN(229)
Ca 25 SWSRNGFIAYI(166)IVAFKWL(51)ACVALRKNGQF(50)INVYSIDINW(163)SFLIIYKDGTVDVVD(250)
Nc 82 AWSRQGTIASI( 94)VVGCYWL(51)ALVSITTNGLL(50)LRIVKATISW(151)VVCFAFSDGTIQYRD(202)
Af 52 AWSKLGCIAYI( 90)IVGMMWL(38)GLVCVTRSGII(50)LCLYRVHIAW(160)VLAITYDDGSVVFYD(200)
Dm 26 SVSARNIVAFS(101)IIEAQFF(41)GCVLLPSSGLL(65)VQCFKVSLSL(121)QVYAILQDNSVQVLE(185)
Hs 38 AWSCRNLITFT( 82)IVALSWL(40)GWIAVTVSGLV(52)VQFYKVCVSV(132)GLALAFHDGSVHIVH(213)
*** +**+ ** + ** ** * * * * **++ * * * + * *+** +++
EEEEEE HHHHHHHHHHHHHHH ------Zinc finger like----
Sc DLIHSLIPVAKWFVKFITYLTQEI(205)LKRCSRCGSVT(19)KRWPTMYTRLCICSGMLFEM 972
Ca ECIMSIIGNFKWLIDLLVYLNQEL(234)LRVCTRCRAVS( 6)GLWTMVFQRTCMCGNAWVNV 1053
Nc EVVEVLVGCVTWAHTLIGWMVDCL(387)WRRCVRCTAVM(11)FTFVMAQQRKCACGAWWALV 1136
Af DVLRMVLGNTKWALDFSFYVLNEL(189)RRRCVRCCEVS(13)MIVKLGLLRACVCGGMWTLE 900
Dm VMLQSLQQLIQWVTDLALNMLHRL(158)IRRCARCGFVF(13)KAWFSKWLH-CHCGGFWKQV 817
Hs NTLQALQQLLQWVGDFVLYLLASL(162)CKACTRCGCVT(11)KQWEQRWIKNCLCGGLWWRV 836
++* * * * +* * +* * ** * * + * * *+ +* +
Med1/TRAP220
HHHHHHHHHHHHHH HHHHHHHH EEEEE HHHHHHHHHHHHHHHHHHH
Sc 8 VETLDSMIELFKDY( 9)ITRLCQTLGLESFTE( 9)LSTASKIIVIDVDYN(44)DLKAFHNNLKSLYLLDAYS(62)
Ca 6 SALDKSLQLLYNYS( 9)IQKLAQQLKLETFID(17)LSIAGSSILLDIDFE(88)KLGNFPVNLQRLATIDKFT(50)
Sp 22 INWTRYWIDCLSSV( 9)FEYLAKKYGLEVFQD( 8)LSLAGKIILIDITVP(38)NTPLFEKNVKWLATFDHSS(48)
Af 122 KRHIEDVVQLLQTR(10)IERLVQLEGFESIWQ( 3)INIAGNFVDLEIEFH(48)SLAGFHGNLQWLSKHDRLS(30)
Nc 118 KAKRLDEVISILSQ( 9)LERLAQKLELDCMWD(10)LIIAGSALELLIGFT(44)QLDKFSVNFERLAILDKLS(60)
Ce 57 RLDVLGSLVFMKEH(12)VQQLSEGLGEHCVFS( 5)FSIRNPDVTIDIGVA(29)EFSKLRDSITAILSSLPKE(34)
Dm 70 KATLQSALDKLQHY(12)LESLSRQLGLKFMED( 3)LFISTDMFYVEILLD(30)DFADFTVQLEGLSSIYQLN(41)
Hs 55 HQHLVSCLETLQKA(12)LESIARQNGLGSHLS( 5)CYITSDMFYVEVQLD(30)NFDEFSKHLKGLVNLYNLP(41)
+ * *+ * + + * ++ * *+* * + * * *
EEEEE HHHHHHHHHHHHHHHHHHHHHHHH
Sc DKFGIYI(133)NLDLISEILKWVQWSRIVLQNVFK 3577
Ca DRLGVTL(170)LIPILRNFLVLINLCRTVIKSKLT 433
Sp GQLGLRI(141)QIRGILAIVRQYLLLQLILENIKS 359
Af SRLGLSL(233)QLADVFPILRQYALLANLIYKIFN 558
Nc GKVGMRI(204)HLIAMLPYLRQYAFLSTLLENSFK 537
Ce GKYGFYA(161)DLSKLIETLRSEWKHASLWESILA 391
Dm SSVGLVL(182)QVPRIVAFLKKQALFYTLLASCVR 431
Hs GSVGYLT(190)RVPLILNLIRHQVAYNTLIGSCVK 426
*** * * +*+ ** +
Med4/TRAP36
HHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHH
Sc 41 QLYEDLCRYEDTLSKLVESV( 8)IAKDLIRTDEALFENVKLLAEY(9)IDKDSEELDSKTRKILEILNECHDELKAL(19)
Ca 78 PMVQKNVEYERLLNELSEAV(10)KIGQIITCNDVLKQQIEDLNKH(9)LSDRNKILEENSKFILKELVSYRNELKKL(13)
Sp 2 YQRAIDSIEECLNKQLRLSS( 7)LIENWTSLVGHLKTLHSLISNY(5)LQNEISSLLKQDKEIDLQIQDCMREMTSI(16)
Af 1 MNVEFQKTLSNLEDKLNALI(14)AALALLDADDSLTSAIETLRRH(9)LRAEAESLEEKVKGIVRDVVGYEKEIRRI(39)
Nc 1 MDKYLDTRFERVEKALATFI(11)LAEDLVAADRELAAGLKELERH(9)LRQETASLDQQTKDIIGGLWNMRKEVKST(13)
Dd 19 RDISDFQRVSRTLFECFSSL(32)LMNHLIDVDKIYQNTLKKLEKH(6)ITEIQKEIEEKDKLISTLALNLKDIESFL(44)
At 85 RAQALLQQMAVLTSKLFDVS(39)ILSQFNSLQTQLFEAVTELQEI(3)KQKVAREIKSKDSSILAFANKLKDAERVL(39)
Ce 12 LLLESADDLEHIVKMIVDTL(17)IIKLFDSKQDSIKTLLKRVPEF(6)IRTLEAHVEKRDEVIQQLETNLKSCEVAL(21)
Dm 10 RLLLLIDDIEMIAKELIEQA(11)LLDLLVAKDEEFRKMLELAEEQ(6)MDQLRAKVEVHDREIQKLQKSLKDAELIL(21)
Hs 29 RLLSALEDLEVLSRELIEML(16)VLELLIHRDGEFQELMKLALNQ(6)MQVLEKEVEKRDSDIQQLQKQLKEAEQIL(21)
+ * * + * + + * * * +
HHHHHHHHHHHHHH EEEEE HHHHHHHHH
Sc INSTELLDYATKLS( 4)IPPTFDKGA( 7)WPAEDALRRGML 193193
Ca VDVDDILKYAFKLA( 4)APATVANMP( 9)WPAEDSLRRGML 228
Sp VNAETLLDYGRKLS( 4)APPGYNPET(11)WPSEDQMRKTLL 151
Af IDYKLLLDFARRIS(67)TRQVYMIP-( 0)YPMEDRIRMGLM 234
Nc FTTHELLNYAKRIS( 5)PPAHLLYNS(99)WPTEDKVRMGGM 242
Dd VSPEDLISYAHKIS( 4)APFGYQPNA( 9)APQDEMMRSSVL 219
At LKLKDILAYAHKIS( 4)APPEFGAGQ( 9)APQDEQMRASQL 287
Ce VNSEMLIKLAHQIS( 6)APLTWQMGD( 4)FPQEHEFRAGHL 171
Dm VSSEELIKYAHRIS( 6)APLTWCIGD( 4)YPTDIEMRNGLL 163
Hs ISSEEIIKYAHRIS( 6)APLTWVPGD( 4)YPTDLEMRSGLL 187
* ** *+ +** + * * * ** *
Med6
EEEEE EEEEE EEEEE EEEEE
Sc 5 PLDELQWKSPEWIQ(3)LRTENVLDYFAES(0)PFFDKTSNNQVIKMQRQ(27)LEEELMKLDGTEYVLSSVREPD( 0)
Ca 3 SLDEIQWKSPEFIQ(3)LNTNNVLEYFSLS(0)PFYDRTSNNQVLMMQFQ(15)FQSRLSEMTGIEFVIAYTKEPD( 0)
Sp 10 DLTSIQWRMPEWVQ(4)LRTENVLEYFSQS(0)PFYSHKSNNEMLKMQSQ( 8)LNSQLKRLTGIQFVIIHERPPF( 0)
Af 8 SLEEILWRSPPHVQ(5)LHSNNILFYFAES(0)PFFDATSNNASLAIQAN(14)FEGRLRTMQGLEFVVAYDPLQA(14)
Nc 12 PLDEIQWHTPPQFE(2)IHSNSILYYFAQS(0)PFYDKTSNNEVVFQQGL(14)FESRLKEMSGLEFIVAQEPAET( 7)
Dd 16 DLTQVMWRDPLWLQ(3)LNPQTILDYFSYS(0)QFYDKNCNNEQLKMQRL(11)NIFKFRNMDGLEYELIKFVEPS( 0)
At 32 DMTGISFRDQLWIN(3)LDRNYIFDYFALS(0)PFYDTTCNNEILRRRSI( 3)DLSHLSKMTGLEYMLTDATEPN( 0)
Ce 15 NPLHVSFRNPQWPP(2)INKDNVLDYFCNQ(2)AFYEMNSCNQQIRMQNI( 4)VEECLRTMPGIQYVLWYS-QPP( 0)
Dm 9 DHLRLSWHDTQMMA(1)LSPQTVMDYFCRK(2)PFYDHMCNNETVRMQRL( 0)GPEHLHNMIGLEYILLHVAEPI( 0)
Hs 9 NLLGISWVDSSWIP(1)LNSGSVLDYFSER(2)PFYDRTCNNEVVKMQRL( 0)TLEHLNQMVGIEYILLHAQEPI( 0)
* *+ + * + ** ** **+ +*+ * + * * *+**+*
EEEEE EEEEEEE EEEEE
Sc FWVIRKQRRTNNS( 8)EIIPLQDYYIIGANIYQSPTIFKIVQSRLMSTSYHLNSTLE 186
Ca FWIIRKQKRQDPQ( 0)NTVTLQDYYIIGANVYQAPRIYDVLSSRLLASVLSIKNSTD 140
Sp LWVIQKQNRLNEN( 0)EVKPLTVYFVCNENIYMAPNAYTLLATRMLNATYCFQKALT 141
Af VWVIRKQMRRKRA( 4)EVVVLATFFVVGDCIYMAPSVASVVGNRIVRRRWFCFAHVR 164
Nc VWVINKQTRRKRP(13)EIIVHSVYFVVGENIYMAPTLADVLSSRIGAIATAITKTIP 167
Dd FFLIAKQTRISPT( 0)DVLINTLYYVINGNIYQAPELHVVFKSRVSQSISHLSEAFN 149
At LFVFRKQKRDGPE( 0)KVTPMLTYYILDGSIYQAPQLCSVFAARVSRTIYNISKAFT 157
Ce LFIICKQRRNNVT( 0)NVSPIAYYYVINGSVHQAPDMYSLVQSRLLGALEPLRNAFG 141
Dm LYVIRKQHRHNPS( 0)EATPIADYYIIGGTVYKAPDLANVINSRILNTVVNLQSAFE 131
Hs LFIIRKQQRQSPA( 0)QVIPLADYYIIAGVIYQAPDLGSVINSRVLTAVHGIQSAFD 1311
**** ** * + **** *+ ** + ** +** * ++
Med7
EEEEE EEEEE HHHHHHHHHHHHHHHHHHHHHHH
Sc 9 VSSLYPPPPPYVKFFTQSNLE( 34)YLIPPPMPKNQQYRAFGSIWQVK( 28)KIQELRKLLKSLLLNYLELLGVL
Ca 14 ISSLYPPPPPYYKFFTKDNLQ( 43)FLIPPKQPEGPQYRGYGNIWLFE( 63)KIKELHKLMDSLLLNFLELIGIL
Sp 9 LFSAFPPPPPYYKLFTRENIE( 32)LFKKPSCLTSGTYQMFGDTWRLD(140)RAYELRFLSRSLMLNFLELLGIM
Af 9 PLTAFAPPPPLWKHFTQDNLK( 37)FLVPPEIPKSGQYSVFGELQSEQ( 26)HAYYLLKISKSLLLNFLEFVGIL
Nc 11 LASFFPDPPLFWKAFTPSNLQ(138)SLSLLLPLQPPVVPPVGASSTQT( 3)LHLHLKRLSKSLLLNFLELLGIM
At 2 ATATYPPPPPYYRLYKDYSEN( 2)SAPEPPPPIEGTYVCFGGNYTTE( 22)YKNELRSLNRELQLHILELADVL
Ce 9 TVSPFPNPPEYASAYTSDRIN( 3)GSAPPPPHPLTEFKVYGEEYRLE( 20)WKTEMKKLNRSAIVAFFDLVEIL
Dm 8 VSSLPMPPERYIANYTDENIR( 4)PRPPPPPAQHEVYSMFGIQYNND( 20)RRKELKKLNHSLLVNFLDLIDFL
Hs 7 VSALPPPPMQYIKEYTDENIQ( 2)LAPKPPPPIKDSYMMFGNQFQCD( 21)HKKELRKLNMSILINFLDLLDIL
* * *+ *+ + + * ** *+ * +++* ****+ **
HHH HHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
Sc SINPD(0)MYERKVENIRTILVNIHHLLNEYRPHQSRESLIMLLEEQLEYKRGEIREIEQVCKQVHDKLTSIQD 208
Ca SIDPI(0)KYDKKIHDINLILININHLLNTYRPHQSRESLIMLLKNQIDYKFLEIDQINKKCLDIKSKIKNLIN 257
Sp AKAPE(0)QFPSKVENIRVLLLNLHHLINDYRPHQSRESLIMLLEKQLKHEESQVELLRTHNRQMTETLEKYKS 317
Af SIAPE(0)QFQSKVEDLRNLFINAHHLLNLYRPHQARESLIMMMEEQLNRSREEIQQMDKMHAEINGFLEQLKA 209
Nc SLDPA(0)AGSQKAADLRTLFLNFHHVLNEYRPHQAREQLIQLMQERLDQTRAETAANRAVAEKARRVLEGLGS 289
At VDRPS(0)QYAKRIGEISSIFKNLHHLLNSLRPHQARATLIHIMELQIQQRKQAVEDIKRRREEAQRLLKDAYL 163
Ce IRAPD(2)MREEKMVDLHTIFINMHHLINEFRPVQARDSVRILQERQIEELSDICKDFKKYLRDGREVVDDQFQ 172
Dm ILNPD(2)RRTEKIDDISLLFVNMHHLLNEFRPHQARETLRVMMEMQKRQRVETAARFQKHLERVREIVNTAFS 171
Hs IRSPG(2)KREEKLEDLKLLFVHVHHLINEYRPHQARETLRVMMEVQKRQRLETAERFQKHLERVIEMIQNCLA 169
* *+ ** **++++**** ***+***++* *++ + + *
Med8/ARC32
HHHHHHHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHH HHHHHHHHH HHHHHHHH
Sc 31 QALDAVRMRLAQLTHSLRRIRDEM( 6)QWYTLQSQLNVTLSQLVSVTSTL(25)LVTTLLRKKNIPEVDEWMKYV 129
Ca 30 DVLESIRNRLNQIRQSLTKLADQI(13)SYLYFQNQFQVLITQLMSITNQL(25)MLTTLLRKKVAPEVDEWINGA 135
Sp 11 ESLEAIRHRIAQIVQSLTHFLAIL( 7)PWPTIHKNFNLLLSQIHSLSNNL(25)LLTTLLRTKALPEVEEWEANT 110
Af 10 KILEQSRQRLVQLTRSLASLIGSL( 7)SWSSLQSQAGIISNNLVSISEHL(25)TLEQLLRTKLDPRVEDWVSRG 109
Nc 16 KQLEVLRNRFHQLTHSLNSLFNDI( 7)SQESLTASAAILQLSLETIQKLA(25)ILLQLLRKKLEPEVESWVEDA 115
Ce 23 QATDMMIKRVTDAKHIIEELLQML( 6)PWPLMLEKFSTLASFMSSLQSSV(48)LVPEYLRTKPNPEMENEEGML 144
Dm 10 LTLDTVLQRLNDLKLAVLSMIQKL( 7)NWPTFLDNFAIISSHLTGLTKIL(40)IVPDYLRTRPDPITEQKMLQN 124
Hs 10 ASLDALLNQVADLKNSLGSFIYKL( 7)TWPSVLDSFALLSGQLNTLNKVL(40)VVPDHLRTKPDPEVEEQEKQL 124
+* + +* *+ +* * * + * ++ * +* + +* +** * * +*+
Med9/ARC105
HHHHHHHHHHH HHHHHHHHHHHHHHHHHHH HHHHHHHHHHHH HHHHHHHHHHH
Sc 3 LQNNVLNQIHQ(10)KPNAEATKEEFSSAENRDEKDYL( 40)LETLTGSIRHRL(12)EWQDIIHQREQ 131
Sp 3 SARELLRKVKQ( 5)KRGLKASSQELKRQKTRDHKSFE( 41)IAKNKELIEEEW(43)EVYDILYEEES 148
Ce 11 FREHVIQRLEP(15)GDARQVEEYVFAKCMSKDEYMRT(642)YDDLQNSVYERL(13)AWEEQVNQYQN 737
Dm 10 FRQNVISKIHD(10)KNAGVMENHIFRKSRTKDEYLGL(642)LQTVQQALIARI(14)TWEMAVRQACS 732
Hs 15 FRQKLVSQIED(10)KSSKDMESHVFLKAKTRDEYLSL(648)LQSVHRCMTSRL(14)TWAQSVHQACL 743
+ + ** * + + * + +**+ *+ + * +* * + * *
Med10/Nut2
EEEEE HHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
Sc 83 VVQYIEDGRNPDIYTREFVEAIRRSNQYQRGKMHGLKQLRDSLADKIVDEFPELKEPVEDIIKRT 147
Ca 86 VISYIEDGRNPDIYTREFIEVNAKSNARLKGKMLGFKKLRDVFGDKLKQEFPQLEKGVDDIINRT 150
Sp 67 VLEYIEQGRNPDVYARQFSELVQKDNQYVNGKLYAIEGFQKAFAEEIKQAYPEVSSVVDKILNEG 131
Af 54 IIDYVDAARNPDIYTREFVELVQRGNQDLKGKKEAFASFRDVLAREMRSAMPECRGEVERVLAAT 118
Nc 120 LITYVENGRNPDVYTREFIELVRRSNQLMRGKMHAFRDFRDVLAGEMEAALPELREDIKRVVEAT 184
Dd IFRVIDQSKNPDLYVKETLQNCLSANEKTKGKIESIKNFKKELESHISESFASEYAEYKLLTQKK >135
At 100 VLNLIDDGKNPDEFTKDVLNSCIARNQVTKGKTDAFKDLRKHILEELEQNFPDEVDMYREIRASS 164
Ce 106 LLPYLDDGKNPCLYSKHCMEKTLEKNKAVNGKIEIYKKFRAHLMKEFSEEMPDLVMYYRSIREDL 170
Dm 68 FFDYIDQDKNPQLYTKDCVEKALAKNEEVKGKIEGLKKFKTNLLLELYKTFPNEMNNYRAYRKDS 132
Hs 67 VFEYIDQGRNPQLYTKECLERALAKNEQVKGKIDTMKKFKSLLIQELSKVFPEDMAKYRSIRGED 131
** ***+ ***++*+*+++* * ** * *+ * * *++ * *
Med11/TRAP25
HHHHHHHHHHHHHHHHHHH HHHHHHHHHHHHHHHHHHH EEEEE
Sc 21 YIQERLKSLNDIETQLCSMLQ(33)LDKSTTQLRKEIQLLDENVG---( 2)LLPINVN( 9)
Ca 12 FIQERLDSLHEIDCKVVTLLD(27)LSKVAIDLRKEIKIMDDNIGAYD( 7)ILPISNV( 9)
Sp 16 QQSESVEVIKRHFREFRKIWE(19)EKAVQEYIRLNNRRYRNKIASGE( 1)WLPIAQE(115)
At 17 LAMEGEKQLEETIEAAFQIIS(55)GGSGNSVLDEASLRYKNSVTSLR( 4)AIPNSQK( 37)
Dm 21 LGQETVQDIASRFQEVFASLK(12)EKKVQEYFRTIRLLFKRVRIIYE(14)LIPYRDE( 31)
Hs 36 IGQETVQDIVYRTMEIFQLLR(18)LTKLQDNLRQLSVLFRKLRLVYD(16)LIPYVEE( 33)
+ * + * ++ +* *+ * +**+ +
HHHHHHHHHHHHHHHHHH
Sc EKMEEQLDLLSAILDPSKSK 131
Ca KRLDLELAELKRLISDEKQV 125
Sp FIFVVVYLLFKRIVWPILSM 220
At VHVKELIDKLRELIADISTW 183
Dm RQLREIIDRTRIIIWEINTM 148
Hs QQLKQIMDQLRNLIWDINAM 176
* * ++ ** ++
Soh1
HHHHHHHHHHHHH HHHHHHHHHH EEEEE HHHHHHHHH
Sc 22 RFEVELEFIQSLANIQYVTY(1)LTQQQIWKSPNFKNYLKYLE(0)YWCNPPYSQCIVYP(0)NCLFILKLL
Ca 30 RWEIELEFVQSLSNIPYVNY(0)LAQNNYFNDENFINYLNYLQ(0)YWTQPEYSKFLVYP(0)NCLHILKLL
Sp 16 RFEIELEFVQMLSNPWYLNF(0)LAQHKYFEDEAFLQYLEYME(0)YWREPEYVKFIIYP(0)TCLHMLTLL
Af 21 RFTLELEFVSSLANPYYLSH(0)LAG-PDPEAQAFAAYLAYLY(1)YWKTPEYAQFLTHP(1)ATLRALRLL
Nc 33 RFELELEFVQALGNPLYLNH(0)LASRKLLSNPAFIAYLDYLQ(0)YWSRPPYLKYITYP(1)PTLKNLELL
Dd 55 RFIMELEFIQCLSNPRYLNY(0)LAQNRYFFDKAFVNYLVYLQ(0)YWKKPEYAKFIVYP(0)QSLYFLDLL
At 31 RFLLELEFIQCLANPTYIHY(0)LAQNRYFEDEAFIGYLKYLQ(0)YWQRPEYIKFIMYP(0)HCLYFLELL
Ce 10 RFEVECEFVQALANPNYLNF(0)LAQRGYFKEEYFVNYLKYLL(0)YWKDPQYARCLKFP(0)QCLHMLEAL
Dm 21 RWQIELEFVQCLSNPNYLNF(0)LAQRGFFKDQSFINYLKYLQ(0)YWKEPDYAKYLMYP(0)MCLYFLDLL
Hs 17 RFQLELEFVQCLANPNYLNF(0)LAQRGYFKDKAFVNYLKYLL(0)YWKDPEYAKYLKYP(0)QCLHMLELL
** ******+ *+* ** ** ++ * ** ** ** * * ** +* * * +*
HHHHHHHHHHH EEEEE
Sc NGFMESAIVNE(18)PQWMNEMVERWAN 127
Ca QQDENRKNIIN( 4)NSLMNDMVKRWQL 120
Sp KNPQFRNDISR( 4)KQVNDEIYYEWLG 106
Af QEESFRRDIIR(13)NVQGGQNVEAGDT 121
Nc QQEKFRQDIIS( 7)RLEGMKAGIDWHR 127
Dd QEERFRQELNH( 4)DFIHEQQFYHWQY 145
At QNPNFRTAMAH( 4)ELAHRQQFYYWKN 121
Ce QSQQFRDSMAY( 4)KFVEDQVVLQWQF 100
Dm QYEHFRREIVN( 4)KFIDDQAILQWQH 111
Hs QYEHFRKELVN( 4)KFIDEQQILHWQH 107
* + * + + +
1