Supporting Information

Organotellurium-bridged cyclodextrin dimers as Artificial Glutathione Peroxidase models

Aiquan Jiaoa,b, Na Yang a,b, Jinpeng Wang a,b, Alhassane Toure a,b, Xueming Xu a,b, and Zhengyu Jina,b,

aThe State Key Laboratory of Food Science and Technology and bSchool of Food Science and Technology, Jiangnan University, 1800 Lihu Road, Wuxi 214122, China

*Corresponding author ()

  1. Kinetic Study of Peroxidase Reaction of 2-TeCD (Fig.1-10)

Fig. 1Lineweaver-Burk plots of [E]0/v0 (min) vs 1/[H2O2] (mM-1) for 2-Te-α-CD (2μM), in 50 mM potassium phosphate buffer, pH 7.0, and 37 °C.

Fig. 2Lineweaver-Burk plots of [E]0/v0 (min) vs 1/[GSH] (mM-1) for 2-Te-α-CD(2μM), in 50 mM potassium phosphate buffer, pH 7.0, and 37 °C.

Fig. 3Lineweaver-Burk plots of [E]0/v0 (min) vs 1/[t-BuOOH] (mM-1) for 2-Te-α-CD(2μM), in 50 mM potassium phosphate buffer, pH 7.0, and 37 °C.

Fig. 4Lineweaver-Burk plots of [E]0/v0 (min) vs 1/[GSH] (mM-1) for 2-Te-α-CD(2μM), in 50 mM potassium phosphate buffer, pH 7.0, and 37 °C.

Fig. 5Lineweaver-Burk plots of [E]0/v0 (min) vs 1/[H2O2] (mM-1) for 2-Te-γ-CD (2μM), in 50 mM potassium phosphate buffer, pH 7.0, and 37 °C.

Fig.6Lineweaver-Burk plots of [E]0/v0 (min) vs 1/[GSH] (mM-1) for 2-Te-γ-CD(2μM), in 50 mM potassium phosphate buffer, pH 7.0, and 37 °C.

Fig.7Lineweaver-Burk plotsof [E]0/v0 (min) vs 1/[t-BuOOH] (mM-1) for 2-Te-γ-CD (2μM), in 50 mM potassium phosphate buffer, pH 7.0, and 37 °C.

Fig.8Lineweaver-Burk plots of [E]0/v0 (min) vs 1/[GSH] (mM-1) for 2-Te-γ-CD (2μM), in 50 mM potassium phosphate buffer, pH 7.0, and 37 °C.

Fig.9Lineweaver-Burk plots of [E]0/v0 (min) vs 1/[CuOOH] (mM-1) for 2-Te-γ-CD (2μM), in 50 mM potassium phosphate buffer, pH 7.0, and 37 °C.

Fig.10Lineweaver-Burk plotsof [E]0/v0 (min) vs 1/[GSH] (mM-1) for 2-Te-γ-CD (2μM), in 50 mM potassium phosphate buffer, pH 7.0, and 37 °C.

2. 1H NMR Study on the Inclusion Complex of GSH with2-Te-γ-CD (Fig. 11-13)

Fig.111H NMR spectra as a function of molar ratios of GSH/2-Te-γ-CD ranging from 1:0 (a) to 1:9 (j) at room temperature.

Fig. 12 Curve-fitting analysis of the differential shift of H5 proton of GSH (Δδobs) to calculate stoichiometry and the complex stability constant (Kc) upon addition of 2-Te-γ-CD (0–9 mM) in D2O at temperature.

Fig. 13 Plots of the shift values of the H5 signal of GSH by adding2-Te-γ-CD versus the molar concentration ratio of 2-Te-γ-CD to GSH atroom temperature.

Corresponding authorat:School of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu provinces, China.Tel./fax: +86 510 85913299.

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