Figure 2. Neighbor-joining tree based on 16S rRNA gene sequences showing the phylogenetic positions of 185 clones. The 16S rRNA gene sequences, which were referred to for the alignment of the sequencing 16S r RNA gene from 16S rRNA gene clone library, were retrieved from Genbank of NCBI and their corresponding accession numbers are demonstrated in parenthesis. Bootstrap values, expressed as a percentage of 1000 replications, are given at branching nodes. The scale bar indicates the 0.002 divergence.

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Figure 3. Neighbor-joining tree based on 16S rRNA gene sequences showing the phylogenetic positions of the clones belonging to Acidobacteria. The 16S rRNA gene sequences, which were referred to for the alignment of the sequencing 16S r RNA gene from 16S rRNA gene clone library, were retrieved from Genbank of NCBI and their corresponding accession numbers are demonstrated in parenthesis. Bootstrap values, expressed as a percentage of 1000 replications, are given at branching nodes. The scale bar indicates the 0.002 divergence.

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Figure 4. Neighbor-joining tree based on 16S rRNA gene sequences showing the phylogenetic positions of the clones belonging to Proteobacteria. The 16S rRNA gene sequences, which were referred to for the alignment of the sequencing 16S r RNA gene from 16S rRNA gene clone library, were retrieved from Genbank of NCBI and their corresponding accession numbers are demonstrated in parenthesis. Bootstrap values, expressed as a percentage of 1000 replications, are given at branching nodes. The scale bar indicates the 0.002 divergence.

Tables

Table 1. Diversity and relative abundance of the bacterial communities in Mo-he permafrost soils based on the 16S rDNA gene sequences from 266 clones.

Prokaryotic group / Nearest phylogenetic relative & it’s accession number / Identity
% / No. of clones / Proportion
%
Acidobacteria / Uncultured Acidobacteria bacterium clone HEW_08_323
HQ598557.1 / 97 / 1 / 0.38
Uncultured Acidobacteria bacterium clone KBS_T1_R4_149264_f4
HM062465.1 / 99 / 7 / 2.63
Uncultured Acidobacteria bacterium clone SEW_08_287
HQ598995.1 / 96 / 1 / 0.38
Uncultured Acidobacteria bacterium clone M-B250
JN038970.1 / 98 / 6 / 2.26
Uncultured Acidobacteria bacterium clone SEW_08_251
HQ598963.1 / 99 / 3 / 1.13
Uncultured Acidobacteria bacterium clone QEDN7BD04
CU927113.1 / 98 / 10 / 3.76
Uncultured Acidobacteria bacterium clone SEW_08_182
HQ598902.1 / 98 / 16 / 6.02
Uncultured Acidobacteria bacterium clone F04_WMSP1
DQ450716.1 / 97 / 6 / 2.26
Uncultured Acidobacteria bacterium clone HEW_08_231
HQ598507.1 / 95 / 1 / 0.38
Uncultured Acidobacteria bacterium clone SEW_08_034
HQ598775.1 / 97 / 1 / 0.38
Uncultured Acidobacteria bacterium clone 2OL3_3
GQ342341.1 / 95 / 6 / 2.26
Uncultured Acidobacteria bacterium clone KBS_T8_R3_149249_a4
HM062040.1 / 98 / 1 / 0.38
Uncultured Acidobacteria bacterium clone KBS_T8_R1_149288_f11
HM061766.1 / 97 / 6 / 2.26
Uncultured Acidobacteria bacterium clone KBS_T8_R1_149288_c5
HM061755.1 / 98 / 8 / 3.01
Uncultured Acidobacteria bacterium clone JG36-GS-69
AJ582046.1 / 95 / 7 / 2.63
Proteobacterium / Uncultured alpha proteobacterium clone GC0AA5ZF09PP1
JQ919489.1 / 94 / 1 / 0.38
Uncultured alpha proteobacterium clone JG37-AG-102 AJ518774.1 / 97 / 4 / 1.50
Uncultured alpha proteobacterium clone SNNP_2012-6 JX114339.1 / 95 / 3 / 1.13
Uncultured alpha proteobacterium clone JG37-AG-1 AJ518761.1 / 97 / 4 / 1.50
Uncultured delta proteobacterium clone ANTXXIII_706-4_Bac6
FN429803.1 / 98 / 3 / 1.13
Uncultured delta proteobacterium clone UMAB-cl-183 FR749808.1 / 97 / 2 / 0.75
Uncultured Methylococcales bacterium clone KWJ_B01
GU572365.1 / 99 / 1 / 0.38
Uncultured gamma proteobacterium clone FAC29 DQ451468.1 / 98 / 4 / 1.50
Uncultured gamma proteobacterium clone A450 JF833520.1 / 98 / 4 / 1.50
Uncultured beta proteobacterium clone:84 AB252927.1 / 97 / 4 / 1.50
Uncultured Gallionella sp. clone: 472 AB252929.1 / 99 / 14 / 5.26
Uncultured Oxalobacteraceae bacterium clone Amb_16S_1099
EF018473.1 / 97 / 1 / 0.38
Uncultured beta proteobacterium clone fjc-41 JQ278786.1 / 98 / 4 / 1.50
Uncultured beta proteobacterium clone 54 HQ462519.1 / 98 / 5 / 1.88
Uncultured Methylobacillus sp. clone R15-12 JF808845.1 / 97 / 4 / 1.50
Verrucomicrobia / Uncultured Verrucomicrobia bacterium clone MVS-16
DQ676318.1 / 98 / 9 / 3.38
Uncultured Verrucomicrobia bacterium clone KWK12F.70
JN656840.1 / 98 / 11 / 4.14
Uncultured Verrucomicrobia bacterium clone A07_WMSP1
DQ450782.1 / 98 / 4 / 1.50
Uncultured Verrucomicrobia bacterium clone Amb_16S_1030
EF018414.1 / 97 / 10 / 3.78
Uncultured Verrucomicrobia bacterium clone E153
JF833884.1 / 96 / 1 / 0.38
Bacteroidetes / Uncultured Bacteroidetes bacterium clone MVP-85
DQ676360.1 / 95 / 1 / 0.38
Uncultured Bacteroidetes bacterium clone IRD18D05
AY947931.1 / 99 / 4 / 1.50
Uncultured Mucilaginibacter sp. clone A199 JF833495.1 / 97 / 3 / 1.13
Uncultured Bacteroidetes bacterium clone: VE-31-3-3-11
AB748640.1 / 99 / 8 / 3.01
Uncultured Bacteroidetes bacterium clone Alchichica_AQ1_2_1B_156
JN825482.1 / 97 / 6 / 2.26
Uncultured Bacteroidetes bacterium clone P-R4
JN038795.1 / 94 / 4 / 1.50
Chloroflexi / Uncultured Chloroflexi bacterium clone Z17M70B FJ484472.1 / 94 / 5 / 1.88
Chlorobi / Uncultured Chlorobi bacterium clone AKYH1119 AY921840.1 / 94 / 7 / 2.63
Uncultured Chlorobi bacterium clone 1OL9 GQ342332.1 / 86 / 1 / 0.38
Actinobacteria / Uncultured Actinobacteria bacterium clone AKYG699
AY921659.1 / 98 / 6 / 2.26
Uncultured Cellulomonadaceae bacterium clone Elev_16S_1338
EF019959.1 / 96 / 4 / 1.50
Actinobacterium YJF1-30
FJ405885.1 / 98 / 5 / 1.88
Syntrophorhabdaceae / Uncultured Syntrophorhabdaceae bacterium clone MBT13
FJ538126.1 / 98 / 4 / 1.50
Uncultured Syntrophorhabdaceae bacterium clone Agri_anode1_161
JN540172.1 / 88 / 1 / 0.38
Nitrospira / Uncultured Nitrospira sp. clone sf-32 JQ278947.1 / 99 / 5 / 1.88
Uncultured Nitrospirae bacterium clone H06_WMSP2 DQ450808.1 / 98 / 2 / 0.75
Firmicutes / Uncultured Clostridium sp. clone ObedB-2D EU073770.1 / 98 / 4 / 1.50
Fibrobacteres / Uncultured Fibrobacteres bacterium clone GASP-WC1S3_A05
EF074547.1 / 70 / 5 / 1.88
Planctomycetales / Planctomycetales bacterium Ellin7244 AY673410.1 / 99 / 5 / 1.88
Gemmatimonadales / Uncultured Gemmatimonadales bacterium clone Amb_16S_1803
EF019156.1 / 83 / 3 / 1.13
Uncultured candidate division / Uncultured candidate division TM7 bacterium clone AMBE4
AM935752.1 / 98 / 4 / 1.50
Uncultured candidate division OP10 bacterium clone D15_23
EU266862.1 / 94 / 1 / 0.38
Uncultured candidate division OP11 bacterium clone DA26
AY193188.1 / 99 / 4 / 1.50

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