Supporting Information

Table S1 Species-specific allele size ranges and allele sizes for jaguars, pumas, and ocelots in Belize using 14 microsatellite loci. n, number of individuals genotyped. Analysis is based on fecal DNA samples, obtained from captive facilities and study sites in Belize. Allele range is measured in base pairs (bp). Species-specific alleles are in bold. a-bmicrosatellite repeat motif – adinucleotide, btetranucleotide

Panthera onca / Puma concolor / Leopardus pardalis
Primer Name / n / Allele range (bp) / Alleles / n / Allele range (bp) / Alleles / n / Allele range (bp) / Alleles
F124b / 72 / 186-204 / 186, 190, 194, 198, 200, 204 / 48 / 202-246 / 202, 206, 210, 214, 216, 218, 220, 222, 224, 226, 230, 232, 234, 238, 246 / 29 / 153-181 / 153, 157, 161, 165, 169, 173, 177, 181
FCA391b / 68 / 203-223 / 203, 207, 211, 215, 219, 223 / 46 / 185-199 / 185, 187, 189, 191, 193, 195, 199 / 23 / 187-209 / 187, 199, 201, 205, 209
FCA043a / 69 / 118-124 / 118, 120, 124 / 47 / 118-140 / 118, 120, 124, 126, 132, 134, 136, 138, 140 / 28 / 113-123 / 113, 115, 117, 119, 123
FCA275a / 73 / 124-134 / 124, 130, 134 / 54 / 126-142 / 126, 132, 134, 136, 138, 140, 142 / 26 / 124-138 / 124, 126, 128, 130, 132, 134, 136, 138
FCA096a / 70 / 178-200 / 178, 182, 188, 192, 194, 198, 200 / 53 / 191-211 / 191, 201, 203, 205, 207, 209, 211 / 24 / 175-195 / 175, 181, 183, 185, 193, 195
FCA126a / 71 / 153-163 / 153, 155, 157, 159, 161, 163 / 52 / 131-141 / 131, 133, 135, 137, 139, 141 / 30 / 119-135 / 119, 121, 123, 125, 127, 129, 131, 135
FCA090a / 71 / 107-115 / 107, 109, 111, 113, 115 / 52 / 91-113 / 91, 93, 99, 101, 103, 105, 109, 111, 113 / 29 / 93-103 / 93, 95, 97, 99, 101, 103
F85b / 66 / 152-172 / 152, 156, 160, 172 / 41 / 152-196 / 152, 156, 160, 164, 168, 176, 180, 184, 196 / 29 / 163-189 / 163, 167, 171, 175, 179, 183, 185, 189
F98b / 67 / 168-174 / 168, 172, 174 / 46 / 163-175 / 163, 173, 175 / 30 / 160-180 / 160, 166, 168, 174, 176, 180
FCA741b / 61 / 148-160 / 148, 156, 160 / 49 / 143-185 / 143, 147, 153, 157, 161, 163, 165, 169, 183, 185 / 4 / 147-185 / 147, 161, 177, 183, 185
FCA225a / 66 / 229-243 / 229, 233, 239, 241, 243 / 48 / 215-245 / 215, 229, 235, 245 / 23 / 217-243 / 217, 227, 229, 231, 233, 235, 237, 239, 241, 243
FCA008a / 69 / 110-126 / 110, 114, 116, 126 / 52 / 129-163 / 129, 131, 149, 161, 163 / 30 / 124-138 / 124, 126, 128, 130, 132, 134, 138
F53b / 62 / 142-182 / 142, 146, 162, 166, 170, 174, 178, 182 / 35 / 180-262 / 180, 182, 186, 190, 194, 198, 230, 234, 238, 242, 250, 254, 258, 262 / 26 / 142-158 / 142, 146, 150, 154, 158
FCA441b / 61 / 141-157 / 141, 145, 147, 149, 153, 157 / 43 / 131-155 / 131, 141, 143, 147, 151, 155 / 21 / 119-131 / 119, 127, 131