Cells are the Starting Point
All living organisms on Earth are divided in pieces called cells. There are smaller pieces to cells that include proteins and organelles. There are also larger pieces called tissues and systems. Cells are small compartments that hold all of the biological equipment necessary to keep an organism alive and successful on Earth.
A main purpose of a cell is to organize. Cells hold a variety of pieces and each cell has a different set of functions. It is easier for an organism to grow and survive when cells are present. If you were only made of one cell, you would only be able to grow to a certain size. You don't find single cells that are as large as a cow. Also, if you were only one cell you couldn't have a nervous system, no muscles for movement, and using the internet would be out of the question. The trillions of cells in your body make your life possible.
One Name, Many Types
There are many types of cells. In biology class, you will usually work with plant-like cells and animal-like cells. We say animal-like because an animal type of cell could be anything from a tiny microorganism to a nerve cell in your brain. Plant cells are easier to identify because they have a protective structure called a cell wall made of cellulose. Plants have the wall; animals do not. Plants also have organelles like the chloroplast (the things that make them green) or large water-filled vacuoles.
We said that there are many types of cells. Cells are unique to each type of organism. Humans may have hundreds of types of cells. Some cells are used to carry oxygen (O2) through the blood (red blood cells) and others might be specific to the heart. If you look at very simple organisms, you will discover cells that have no defined nucleus (prokaryotes) and other cells that have hundreds of nuclei (multinucleated). The thing they all have in common is that they are compartments surrounded by some type of membrane. Cell Membranes
We have been talking about cells being a unit of organization in biology. Let's look at the cell membrane and see how that membrane keeps all of the pieces inside. When you think about a membrane, imagine it is like a big plastic bag with some tiny holes. That bag holds all of the cell pieces and fluids inside the cell and keeps any nasty things outside the cell. The holes are there to let some things move in and out of the cell.
Flexible Containers
The cell membrane is not one solid piece. Everything in life is made of smaller pieces and a membrane is no different. Compounds called proteins and phospholipids make up most of the cell membrane. The phospholipids make the basic bag. The proteins are found around the holes and help move molecules in and out of the cell.
Scientists describe the organization of the phospholipids and proteins with the fluid mosaic model. That model shows that the phospholipids are in a shape like a head and a tail. The heads like water (hydrophilic) and the tails do not like water (hydrophobic). The tails bump up against each other and the heads are out facing the watery area surrounding the cell. The two layers of cells are called the bilayer.
Ingrained in the Membrane
What about the membrane proteins? Scientists have shown that the proteins float in that bilayer. Some of them are found on the inside of the cell and some on the outside. Other proteins cross the bilayer with one end outside of the cell and one end inside. Those proteins that cross the layer are very important in the active transport of ions and small molecules.
Many Membranes
As you learn more about the organelles inside of the cell, you will find that most have a membrane. They do not have the same chemical makeup as the cell membrane. Each membrane is unique to the organelle. The membrane that surrounds a lysosome is different from the membrane around the endoplasmic reticulum. They are both different from the cell membrane. Some organelles have two membranes. A mitochondrion has an outer and inner membrane. The outer membrane contains the mitochondrion parts. The inner molecule holds digestive enzymes that break down food. While we talk about membranes all the time, you should remember they all use a basic phospholipid bilayer, but have many other different parts. Cell Nucleus - Commanding the Cell
The cell nucleus acts like the brain of the cell. It helps control eating, movement, and reproduction. If it happens in a cell, chances are the nucleus knows about it. The nucleus is not always in the center of the cell. It will be a big dark spot somewhere in the middle of all of the cytoplasm (cytosol). You probably won't find it near the edge of a cell because that might be a dangerous place for the nucleus to be. If you don't remember, the cytoplasm is the fluid that fills cells.
Life Before a Nucleus
Not all cells have a nucleus. Biology breaks cell types into eukaryotic (those with a defined nucleus) and prokaryotic (those with no defined nucleus). You may have heard of chromatin and DNA. You don't need a nucleus to have DNA. If you don't have a defined nucleus, your DNA is probably floating around the cell in a region called the nucleoid. A defined nucleus that holds the genetic code is an advanced feature in a cell.
Important Materials in the Envelope
The things that make a eukaryotic cell are a defined nucleus and other organelles. The nuclear envelope surrounds the nucleus and all of its contents. The nuclear envelope is a membrane similar to the cell membrane around the whole cell. There are pores and spaces for RNA and proteins to pass through while the nuclear envelope keeps all of the chromatin and nucleolus inside.
When the cell is in a resting state there is something called chromatin in the nucleus. Chromatin is made of DNA, RNA, and nuclear proteins. DNA and RNA are the nucleic acids inside of the cell. When the cell is going to divide, the chromatin becomes very compact. It condenses. When the chromatin comes together, you can see the chromosomes. You will also find the nucleolus inside of the nucleus. When you look through a microscope, it looks like a nucleus inside of the nucleus. It is made of RNA and protein. It does not have much DNA at all.
Mitochondria - Turning on the Powerhouse
Mitochondria are known as the powerhouses of the cell. They are organelles that act like a digestive system that takes in nutrients, breaks them down, and creates energy for the cell. The process of creating cell energy is known as cellular respiration. Most of the chemical reactions involved in cellular respiration happen in the mitochondria. A mitochondrion is shaped perfectly to maximize its efforts.
Mitochondria are very small organelles. You might find cells with several thousand mitochondria. The number depends on what the cell needs to do. If the purpose of the cell is to transmit nerve impulses, there will be fewer mitochondria than in a muscle cell that needs loads of energy. If the cell feels it is not getting enough energy to survive, more mitochondria can be created. Sometimes they can even grow, move, and combine with other mitochondria, depending on the cell's needs.
Mitochondria Structure
Mitochondria have two membranes (not one as in other organelles). The outer membrane covers the organelle and contains it. The inner membrane folds over many times (cristae). That folding increases the surface area inside the organelle. Many of the chemical reactions happen on the inner membrane of the mitochondria. The increased surface area allows the small organelle to do as much work as possible. If you have more room to work, you can get more work done. Similar surface area strategies are used by microvilli in your intestinal cells. The fluid inside of the mitochondria is called the matrix.
Using Oxygen to Release Energy
How are mitochondria used in cellular respiration? The matrix is filled with water (H2O) and proteins (enzymes). Those proteins take food molecules and combine them with oxygen (O2). The mitochondria are the only place in the cell where oxygen can be combined with the food molecules. After the oxygen is added, the material can be digested. They are working organelles that keep the cell full of energy. A mitochondrion may also be involved in controlling the concentration of calcium (Ca) within the cell. Chloroplasts - Show me the Green
Chloroplasts are the food producers of the cell. They are only found in plant cells and some protists. Animal cells do not have chloroplasts. Every green plant you see is working to convert the energy of the sun into sugars. Plants are the basis of all life on Earth. They create sugars, and the byproduct of that process is the oxygen that we breathe. That process happens in the chloroplast. Mitochondria work in the opposite direction and break down the sugars and nutrients that the cell receives.
Special Structures
We'll hit the high points for the structure of a chloroplast. Two membranes contain and protect the inner parts of the chloroplast. The stroma is an area inside of the chloroplast where reactions occur and starches (sugars) are created. One thylakoid stack is called a granum. The thylakoids have chlorophyll molecules on their surface. That chlorophyll uses sunlight to create sugars. The stacks of sacs are connected by stromal lamellae. The lamellae act like the skeleton of the chloroplast, keeping all of the sacs a safe distance from each other and maximizing the efficiency of the organelle.
Making Food
The purpose of the chloroplast is to make sugars and starches. They use a process called photosynthesis to get the job done. Photosynthesis is the process of a plant taking energy from the Sun and creating sugars. When the energy from the Sun hits a chloroplast, chlorophyll uses that energy to combine carbon dioxide (CO2) and water (H2O). The molecular reactions create sugar and oxygen (O2). Plants and animals then use the sugars (glucose) for food and energy. Animals also use the oxygen to breathe.
Different Chlorophyll Molecules
We said that chlorophyll molecules sit on the outside of the thylakoid sacs. Not all chlorophyll is the same. Three types of chlorophyll can complete photosynthesis. There are even molecules other than chlorophyll that are photosynthetic. One day you might hear about carotenoids, phycocyanin (bacteria), phycoerythrin (algae), and fucoxanthin (brown algae). While those compounds might complete photosynthesis, they are not all green or the same structure as chlorophyll. Looking at Cell Functions
All cells have a purpose. If they don't do anything productive, they are not needed anymore. In the big picture, a cell's purpose is much more important than acting as small organizational pieces. They had their purpose long before they started working together in groups and building more advanced organisms. When alone, a cell's main purpose is to survive.
Even if you were a single cell, you would have a purpose. You would have to survive. You would be moving around (probably in a liquid) and just trying to stay alive. You would have all of your pieces inside of you. If you were missing a piece you needed to survive, you would die. Scientists call those pieces organelles. Organelles are groups of complex molecules that help a cell survive.
All Cells are not Created Equal
In the same way that cells survive in different ways; all cells have different types and amounts of organelles. The larger a cell becomes the more organelles it will need. It makes sense if you think about it. If you are a big cell, you will need to eat more than a little cell. You will also need to convert that food into energy. A larger cell would need to eat more and may wind up having more mitochondria to process that food into energy. While they might have a purpose, more advanced cells have a difficult time surviving on their own. A cell from your brain could not survive in a Petri dish for long. It doesn't have the right pieces to live on its own. It does have the ability to transmit electrical systems around your body. An amoeba could survive in a dish forever, thrive, and reproduce. On the other hand, that amoeba will never help you transmit electrical impulses. The brain cell is far more advanced and has specific abilities and organelles. Simpler cells have a better chance of surviving on their own while complex cells can accomplish tasks that are more advanced. PASSIVE TRANSPORT - TAKING THE EASY ROAD
While active transport requires energy and work, passive transport does not. There are several different types of this easy movement of molecules. It could be as simple as molecules moving freely such as osmosis or diffusion. You may also see proteins in the cell membrane that act as channels to help the movement along. And of course there is an in-between transport process where very small molecules are able to cross a semi-permeable membrane.
Sometimes, proteins are used to help move molecules more quickly. It is a process called facilitated diffusion. It could be as simple as bringing in a glucose molecule. Since the cell membrane will not allow glucose to cross by diffusion, helpers are needed. The cell might notice outside fluids rushing by with free glucose molecules. The membrane proteins then grab one molecule and shift their position to bring the molecule into the cell. That's an easy situation of passive transport because the glucose is moving from higher to lower concentration. It's moving down a concentration gradient. If you needed to remove glucose, the cell would require energy.
LETTING CONCENTRATION DO THE WORK
Sometimes cells are in an area where there is a large concentration difference. For example, oxygen molecule concentrations could be very high outside of the cell and very low inside. Those oxygen molecules are so small that they are able to cross the lipid bilayer and enter the cell. There is no energy needed for this process. In this case, it's good for the cell because cells need oxygen to survive. It can also happen with other molecules that can kill a cell.
OSMOSIS
Another big example of passive transport is osmosis. This is a water specific process. Usually, cells are in an environment where there is one concentration of ions outside and one inside. Because concentrations like to be the same, the cell can pump ions in an out to stay alive. Osmosis is the movement of water across the membrane. For a cell to survive, ion concentrations need to be the same on both sides of the cell membrane. If the cell does not pump out all of its extra ions to even out the concentrations, the water is going to move in. This can be very bad. The cell can swell up and explode. The classic example of this type of swelling happens when red blood cells are placed in water. The water rushes in to the cells, they expand and eventually rupture (POP!).
ACTIVE TRANSPORT - ENERGY TO TRANSPORT
Active transport describes what happens when a cell uses energy to transport something. We're not talking about phagocytosis (cell eating) or pinocytosis (cell drinking) in this section. We're talking about the movement of individual molecules across the cell membrane. The liquids inside and outside of cells have different substances. Sometimes a cell has to work and use some energy to maintain a proper balance of ions and molecules.
PROTEINS IN THE MEMBRANE
Active transport usually happens across the cell membrane. There are thousands of proteins embedded in the cell's lipid bilayer. Those proteins do much of the work in active transport. They are positioned to cross the membrane so one part is on the inside of the cell and one part is on the outside. Only when they cross the bilayer are they able to move molecules and ions in and out of the cell. The membrane proteins are very specific. One protein that moves glucose will not move calcium (Ca) ions. There are hundreds of types of these membrane proteins in the many cells of your body.
Many times, proteins have to work against a concentration gradient. That term means they are pumping something (usually ions) from areas of lower to higher concentration. This happens a lot in neurons. The membrane proteins are constantly pumping ions in and out to get the membrane of the neuron ready to transmit electrical impulses.