Leader

-10

p-12 (NS3-12) TTTTCCTCCTGATTTTCACAAGAACCTTCACG ATG ATT GAA CGG GTT CGA GCA CTC GCG GCT CTG CTG TTC TGT CTC CAC AGT GCA AAT

M I E R V R A L A A L L F C L H S A N

adult ......

p-32 (NS3-8) AATCTACAAC ATG AAC GAG TGG CTT CGT GTT CTC GCC GCC CTG GTA CTT GGT TTG CAA TGT ACT GAC

M N E W L R V L A A L V L G L Q C T D

FR1 CDR1FR2

-1 1 11 20 30

pup-12GGG GAT CCT GTT CTG ACT CAG CCA GGT TCC ATC TCA ACA TCC CCG GGC AAA ACT GTG ACG ATT ACC TGT ACC ATG TCC GGA GGC AGC ATC GGC AGC TAC TAC ACG AGC TGG TAC TGG CAG

G D P V L T Q P G S I S T S P G K T V T I T C T M S G G S I G S Y Y T S W Y W Q

adult...... T ...... CT......

pup-32GCT GGA ACC GTA CTG ACT CAG CCA GGG TCC ATC TCA AGC TCA TTG GGG AAA ACG GTC ACA ATT ACT TGT ACC ATA TCC GGA GGC AGC ATA GGC AGC TAC CGC ACG AGC TGG TAC TGG CAG

A G T V L T Q P G S I S S S L G K T V T I T C T I S G G S I G S Y R T S W Y W Q

CDR2 FR3

40 50 60 70

pup-12AAA CCC AAA AGT GCC CCG GTC TTT GTT TGG CAC GAA AGC ATA GGT ACA --- GCT TCG GGA ATT CCA GAT CGA TTT ACC GGG TCC GTG GAC TCG TCA AGT AAC AAG ATG CAT TTA ACC ATC

K P K S A P V F V W H E S I G T - A S G I P D R F T G S V D S S S N K M H L T I

adult...... CGA ...... A ...... T. --- ..C G.. .AG ......

pup-32AAA CGC GAT AGT GCC CCC GCC TAT GTT TGG AAC GAT TAT GAT TCT AGC AGA GGC CCT GGG ATT CCT GTT CGA TTC ACT GGG TCC AGG GAC ACG TCG AAG AAC CTT ATG CAT CTA ACT ATT

K R D S A P A Y V W N D Y D S S R G P G I P V R F T G S R D T S K N L M H L T I

CDR3 FR4 Constant Region

80 90 100 110

pup-12ACA ACC GTA CAG TCG GAG GAC GCC GCC GAT TAT TAT TGC GCT GTA GCA GAA AGC GGA ATA ------GTT ACA TTC GGG AAA GGA ACC AAG CTG AAC CTG GGC AAT CCA CGA GCG CCT TCA

T T V Q S E D A A D Y Y C A V A E S G I - - V T F G K G T K L N L G N P R A P S

adult...... G ...... G.. .C......

pup-32TCC AAT GTA CAG ACC GAA GAT GTC GCT GAC TAT TAT TGC GGC GCC TGG CAT GCC TCC TAT GGT GGA TTC ACT TTC GGG AAA GGA ACG AAA CTG AGT GTG GGC GAT CCG CGG CCT CCT TCT

S N V Q T E D V A D Y Y C G A W H A S Y G G F T F G K G T K L S V G D P R P P S

120 130 140 150

pup-12CTT TCC CTC CTT CCA CCT TCA CCG GTT CAA ATC ACG GAA AAG AGC AAG GCG ACC TTG GTG TGT TTG GTG AGC GGT TTT AAC CCG GGA GCT GCG GAG ATT CAG TGG ACG GTA GAC GGA AAT

L S L L P P S P V Q I T E K S K A T L V C L V S G F N P G A A E I Q W T V D G N

adult......

pup-32GTC TCC CTC CTT CCG CCT TCA TCG GTT CAA ATT ACA GAA AAG AAC AAA GCG ACC CTG GTG TGT TTG GTC AGC GGT TTT ATC CCG GGC GCT GTG GAG ATT GAG TGG ACG GTA GAC GGC AGT

V S L L P P S S V Q I T E K N K A T L V C L V S G F I P G A V E I E W T V D G S

160 170 180 190

pup-12GTC AGA GGG AAC GGA GTT GAG ACC AGT CGG ATC CAG CAG GAA ACG GAC AAC ACG TTC AGT ATG AGC AGT TAT CTG ACT TTG TCA GCC TCA GAG TGG AAT TCA CAC GAG CTT TAC TCC TGT

V R G N G V E T S R I Q Q E T D N T F S M S S Y L T L S A S E W N S H E L Y S C

adult......

pup-32GGC AGG GGG GAT GCT GTT GAG ACC AGT CGG ATC CAG CAG GAG AAG GAC AAC ACG TTC AGT GTG AGC AGT TTT CTG ACC CTG TCA GCC TCA GAG TGG AAC TCA CAC GAG CTT TAC TCC TGT

G R G D A V E T S R I Q Q E K D N T F S V S S F L T L S A S E W N S H E L Y S C

200 210

pup-12GGG GTT AAA CAC GAG ACT CTA ACA AAC CCA CTT CAA AAA AGC ATC ACG AAA TCT AGT TGT GTT TGATTCAAATATCTAATTTTTTTTATGCTAGCGGCAAGTTTTGTGTAAAATCAAACAAGCTCGGTATAGTAGACA

G V K H E T L T N P L Q K S I T K S S C V

adult......

pup-32GCG GTT AAA CAC GAG ACT CAG GCA ACC CCG TTT AAA TCT AGT GTC AAA AGG TCC AGT TGT GTG TGATGCGAGAATCGTACGTAAACATCTATTGTGCCATGAGTAGACTTCAGTTGAACAGTATTCGAGCTTAAAGGT

A V K H E T Q A T P F K S S V K R S S C V

pup-12AATTCAAATCATAACAGGTGACAATTTCTCTGTGCATATTTCACAGTGTGTTCACTCTGAAGTGGTCAAATCAACGTGCAACTGCTCGCAGTATAATAAAAATGACTATTGCTTATCTC (A20)

adult...... (A)

pup-32TGGAGGGTTTTTTTAAAATTTTAATTGCTGTTTTGGAAATTACCACAATGTGTGGTCAATTTGTTTGTTTGATTCCACTGGGCGTGACTCTGTAATCGTCTCACCTCTATCATTGAGTATAATAAAATAATTCTCCCAC (A18)

Lee et al. Figure 1S