Supplementary Table 1:

ISOLATE NAME
GENE / RVA/Human-wt/IND/MCS-KOL-383/2014 / RVA/Human-wt/IND/MCS-KOL-29/2014 / RVA/Human-wt/IND/MCS-KOL-127/2014 / RVA/Human-wt/IND/MCS-KOL-16/2014
VP1 / KU292519 / KU292530 / KU292541 / KU292552
VP2 / KU292520 / KU292531 / KU292542 / KU292553
VP3 / KU292521 / KU292532 / KU292543 / KU292554
VP4 / KU292522 / KU292533 / KU292544 / KU292555
VP6 / KU292523 / KU292534 / KU292545 / KU292556
VP7 / KU292524 / KU292535 / KU292546 / KU292557
NSP1 / KU292525 / KU292536 / KU292547 / KU292558
NSP2 / KU292526 / KU292537 / KU292548 / KU292559
NSP3 / KU292527 / KU292538 / KU292549 / KU292560
NSP4 / KU292528 / KU292539 / KU292550 / KU292561
NSP5 / KU292529 / KU292540 / KU292551 / KU292562

The GenBank accession numbers relates to the corresponding genes of four unusual rotavirus strains collected in Kolkata, India during 2014.

Supplementary Table 2:

Mixed RVA infections detected by multiplex PCR, which were further confirmed that possibly preceded the emergence of unusual genotypes confirmed by sequencing.

Multiplex PCR based
mixed genotypes / Unusual genotypes confirmed by Sequencing
G1P[4] / G1P[6] / G2P[8] / G9P[4]
G1P[4,8] / 11 / - / - / -
G2P[4,8] / - / - / 26 / -
G9P[4,6] / - / - / - / 21
G9P[4,8] / - / - / - / 15
G1,9P[6] / - / 19 / - / -
Total (n=92) / 11 / 19 / 26 / 36

Supplementary Table 3:

Comparative Nucleotide identity of 11 genome segments of Indian G10P[14] rotavirus strain with V585 Australian G10P[14] rotavirus strain.

Gene
encoding / Identity of V585 against indicated strains
KOL-29 / KOL-383
VP1 / 84.2% / 84.7%
VP2 / 87.4% / 87.1%
VP3 / 86.7% / 87.4%
VP4 / 88.0% / 86.5%
VP6 / 93.4% / 92.9%
VP7 / 85.5% / 85.6%
NSP1 / 74.7% / 78.2%
NSP2 / 91.3% / 85.5%
NSP3 / 89.7% / 91.6%
NSP4 / 88.4% / 87.4%
NSP5 / 94.5% / 94.1%