Supplementary Table 1: Whitefly (Bemisia tabaci) transmissions of Sweet potato leaf curl virus fromwild morning glory (Ipomoea purpurea) showing leaf curl (ILC) and yellow vein (IYV) symptoms

Host plant / Inoculation from ILC / Inoculation from IYV
No. infected /inoculated a / Symptoms b / DPI / No. infected /inoculated / Symptoms / DPI
Rep I / Rep II / Rep I / Rep II
I.purpurea / 2/4 / 3/4 / LC / 20-25 / 2/4 / 2/3 / YV / 20-25
Solanum lycopercisum / 0/6 / 0/6 / NS / - / 0/6 / 0/6 / NS / -
N. benthamiana / 0/5 / 0/6 / NS / - / 0/6 / 0/6 / NS / -

a Whiteflies (10-15/plant) were allowed for 24 h each of acquisition and inoculation access

b LC: leaf curl, YV: yellow vein, NS: No symptom, Rep: Replication

Supplementary Table 2. Percent sequence identities of Sweet potato leaf curl virus isolate obtained from morning glory (Ipomoea purpurea) showing yellow vein symptoms in New Delhi (SPLCV-IYV-1:Del:09 [JX050195]) with the other Ipomoea infecting begomoviruses reported worldwide

Virus/ Isolates / Accession / Host / Country of origin/year / Length of DNA A / DNA A / AV2 / AV1 / AC3 / AC2 / AC1 / AC4
SPLCV-ILC:Del:09 / JX050197 / I.purpurea / India/2012 / 2801 / 97.8 / 97.7 / 100 / 80.5 / 81.0 / 100 / 100
SPLCV-IYV-2:Del:10 / JX050196 / I.purpurea / India/2012 / 2801 / 97.1 / 98.4 / 93.3 / 86.1 / 85.1 / 99.1 / 97.6
SPLCV-[CN:Y338:10] / FN806776 / I. indica / China/2010 / 2801 / 95.3 / 84.7 / 100 / 84.7 / 89.8 / 97.8 / 91.7
SPLCV-[CN:RL7:07] / EU267799 / I.purpurea / China/2007 / 2799 / 95.2 / 85.4 / 100 / 84.0 / 90.5 / 97.5 / 91.7
SPLCV-[CN:RL31:07] / EU253456 / I.purpurea / China/2007 / 2800 / 94.6 / 84.7 / 100 / 85.4 / 91.2 / 97.2 / 91.7
SPLCV-[J:08] / AB433788 / - / Japan/2008 / 2828 / 92.7 / 93.9 / 96.4 / 81.9 / 89.1 / 97.8 / 91.7
SPLCV-[BR:RS2:09] / FJ969834 / I.batatas / Brazil/2009 / 2829 / 92.5 / 67.9 / 96.0 / 82.6 / 91.2 / 97.5 / 87.0
SPLCV-[CN:10] / FJ176701 / I.batatas / China/2008 / 2828 / 92.2 / 85.4 / 96.4 / 81.2 / 87.8 / 98.9 / 92.9
SPLCV-[BR:AM1:09] / HQ393471 / I.batatas / Brazil/2010 / 2829 / 92.4 / 82.4 / 96.0 / 81.9 / 91.2 / 97.2 / 87.0
SPLCV-[BR:AM2:09] / HQ393472 / I.batatas / Brazil/2010 / 2829 / 92.3 / 82.4 / 96.0 / 81.9 / 91.2 / 97.2 / 87.0
SPLCV-[US:98] / AF104036 / - / USA/1998 / 2828 / 92.1 / 83.2 / 96.8 / 82.6 / 89.8 / 96.9 / 87.0
SPLCV-[BR:PA:08] / HQ393450 / I.batatas / Brazil/2010 / 2829 / 92.3 / 82.4 / 96.0 / 81.9 / 91.2 / 97.5 / 87.0
SPLCV-[US:MS:WS3-8:10] / HQ333142 / I.setosa / USA/2010 / 2828 / 91.8 / 84.3 / 97.6 / 81.9 / 85.8 / 96.4 / 92.9
SPLCLaV-[ES:MAL:BG30:06] / EU839579 / I.batatas / Spain/2009 / 2814 / 89.7 / 94.6 / 96.8 / 81.2 / 84.4 / 90.1 / 65.5
SPLCLaV-[ES:CI:BG27:02] / EF456746 / I.batatas / Spain/2009 / 2814 / 89.5 / 94.6 / 96.4 / 82.6 / 85.1 / 89.2 / 64.3
SPLCCaV-[ES:CI:BG25:02] / FJ529203 / I.batatas / Spain/2008 / 2807 / 88.5 / 96.2 / 98.0 / 81.9 / 85.1 / 82.4 / 51.7
SPLCCaV-[ES:CI:BG21:02] / EU856365 / I.batatas / Spain/2009 / 2807 / 88.5 / 95.4 / 97.6 / 81.9 / 85.1 / 82.4 / 51.7
SPLCCaV-[ES:CI:BG7:02] / EF456745 / I.batatas / Spain/2009 / 2807 / 88.4 / 96.2 / 97.6 / 80.5 / 85.1 / 82.4 / 51.7
IYVV-[ES:MAL:IG3:06] / EU839577 / I.indica / Spain/2009 / 2783 / 87.6 / 94.6 / 95.6 / 64.8 / 59.7 / 92.3 / 92.9
IYVV-[ES:MAL:IG5:06] / EU839578 / I.indica / Spain/2009 / 2783 / 85.5 / 93.1 / 92.1 / 65.5 / 58.3 / 92.5 / 89.4
SPLCBV-[IN:BCKV:10] / FN432356 / I.batatas / India/2010 / 2823 / 86.2 / 81.6 / 91.3 / 67.5 / 63.0 / 75.0 / 54.1
SPLCCNV-[CN:05] / DQ512731 / - / China/2006 / 2771 / 80.4 / 77.0 / 93.3 / 68.7 / 62.8 / 66.2 / 81.1
SPLCGoV-[US:Geo16] / AF326775 / I.batatas / USA/2000 / 2773 / 80.7 / 85.4 / 95.6 / 67.5 / 65.1 / 80.4 / 47.0
SPLCESV-[ES:CI:BG1:02] / EF456741 / I.batatas / Spain/2009 / 2780 / 78.5 / 71.7 / 90.5 / 66.2 / 59.0 / 85.9 / 56.4

SPLCV:Sweet potato leaf curl virus, SPLCLaV: Sweet potato leaf curl Lanzarote virus, SPLCCaV:Sweet potatoleaf curl Canary virus, IYVV:Ipomoea yellow vein virus, SPLCBV: Sweet potato leaf curl Bengal virus,SPLCCNV:Sweet potato leaf curl China virus,SPLCGoV:Sweet potato leaf curl Georgia virus, SPLCESV: Sweet potato leaf curlSpain virus, ‘-’ : not known. ILC-Del:Ipomoea purpurea leaf curl-Delhi isolate, IYV-Del:I. purpurea yellow vein-Delhi isolate, Identity of DNA A is based on nucleotide sequence and identity of open reading frames in DNA A is based on amino acid sequence.

Supplementary Table 3. Percent sequence identities of the betasatellite isolates obtained from wild morning glory (Ipomoeapurpurea) showing leaf curl (ILC-beta, JX050198) and yellow vein (IYV-beta, JX050199) symptoms in Delhi with the selected betasatellites

Betasatellitea / Acc. No. / Host / Country of origin/year / Length of betasatellite / With ILC beta / With IYV beta
Full lengthb / BC1c / Full lengthb / BC1c
CroYVMB-[IN:Pan:08] / HM143908 / Papaya / India/2010 / 1358 / 90.6 / 96.6 / 58.2 / 61.8
CroYVMB-[IN:Bar3:08] / GQ183866 / Sun hemp / India/2009 / 1359 / 85.2 / 79.6 / 59.6 / 66.9
CroYVMB-[PK:Pun:06] / AM410551 / Croton / Pakistan/2006 / 1346 / 84.9 / 86.4 / 60.1 / 68.6
CroYVMB-[IN:Pat:08] / FJ593630 / Radish / India/2008 / 1367 / 84.2 / 85.5 / 59.5 / 66.1
CroYVMB-[IN:Raj:10] / HQ631430 / Calotropis / India/2010 / 1391 / 82.4 / 96.6 / 59.8 / 61.8
CroYVMB-[IN:Bhu:09] / GU111995 / Bhendi / India/2009 / 1344 / 82.4 / 86.4 / 58.8 / 68.6
CroYVMB-[IN:Luc:08] / EU604296 / Jatropha / India/2008 / 1315 / 82.0 / 83.0 / 57.7 / 67.7
CroYVMB-[IN:Bar:06] / EF597245 / Croton / India/2007 / 1357 / 80.8 / 79.6 / 58.3 / 66.9
PaLCuB-[IN:ND:03] / AY244706 / Papaya / India/2003 / 1372 / 57.6 / 59.8 / 95.4 / 94.2
PaLCuB-[IN:Chi:05] / DQ118862 / Mungbean / India/2005 / 1367 / 57.3 / 62.7 / 95.6 / 98.3
PaLCuB-[IN:Mb:05] / AY438557 / Mungbean / India/2003 / 1360 / 57.2 / 61.0 / 95.1 / 96.6
ToLCBDB-[IN:Pusa4:10] / HQ180396 / Tobacco / India/2010 / 1377 / 56.8 / 50.8 / 59.2 / 61.0
RaLCuB-[IN:Var:06] / EF175734 / Radish / India/2006 / 1358 / 56.1 / 32.0 / 54.6 / 40.8
ToLCMaB-[IN:Pun:06] / AY754815 / Tomato / India/2004 / 1380 / 55.7 / 63.5 / 75.3 / 76.2
ToLCMaB-[IN:Pun:04] / AY838894 / Tomato / India/2004 / 1380 / 55.4 / 58.4 / 75.0 / 87.2
PaLCuB-[IN:Jab:03] / AY230138 / Tomato / India/2003 / 1424 / 54.5 / 61.8 / 90.4 / 96.6
ToLCBB-[IN:Hes:10] / GU984046 / Tomato / India/2010 / 1339 / 54.3 / 62.7 / 73.7 / 75.4
ToLCBB-[IN:Ban5:07] / EU280314 / Tomato / India/2007 / 1361 / 54.1 / 61.0 / 72.6 / 71.1
TbCSB-[CN:Yn291:04] / AM260735 / Tobacco / China/2006 / 1356 / 54.1 / 31.3 / 53.2 / 26.2
ToLCB-[IN:Coi:03] / AY438560 / Tomato / India/2003 / 1376 / 53.9 / 63.5 / 72.9 / 76.2
ChLCB-[IN:Pat:08] / EU582020 / Chilli / India/2008 / 1380 / 53.9 / 56.7 / 60.1 / 61.0
ToLCJoB-[IN:Var:10] / JF681133 / Chilli / India/2011 / 1369 / 52.9 / 79.6 / 56.4 / 66.9
ToLCPaB-[IN:Pusa2:10] / HQ180394 / Tobacco / India/2010 / 1338 / 52.9 / 79.6 / 56.4 / 66.9
CLCuMB-[IN:Har05:06] / EF614158 / Hibiscus / India/2007 / 1351 / 47.6 / 52.5 / 50.0 / 65.0

aCroYVMB: Croton yellow vein mosaic betasatellite, PaLCuB: Papaya leaf curl betasatellite, ToLCBDB: Tomato leaf curl Bangladesh betasatellite, RaLCuB: Radish leaf curl betasatellite, ToLCMaB: Tomato leaf curl Maharastra betasatellite, ToLCBB:Tomato leaf curl Bangalore betasatellite, TbCSB:Tobacco curly shoot betasatellite, ToLCB:Tomato leaf curl betasatellite, ChLCB: Chilli leaf curl betasatellite, ToLCJoB:Tomato leaf curl Joydebpur betasatellite, ToLCPaB: Tomato leaf curl Patna betasatellite, CLCuMB: Cotton leaf curl Multan betasatellite

bBased on nucleotide sequence, c Based on amino acid sequence. The highest identity is highlighted in bold.