Reagents Needed for the Affymetrix Exon 1

Reagents needed for the Affymetrix Exon 1.0 ST Array Whole Transcript Assay

Updated 8/10/2009

Reagents provided by the Genomics Lab

11 ul of Poly A+ controls, sufficient for 10 reactions £20

Reagents to order directly from Affymetrix

(Please contact the GL to get our customer number for discount)

Arrays

Human

·  GeneChip Human Exon 1.0 ST Array – pack of 2 - P/N 900649

·  GeneChip Human Exon 1.0 ST Array – pack of 6 - P/N 900650

·  GeneChip Human Exon 1.0 ST Array – pack of 30 - P/N 900651

Mouse

·  GeneChip Mouse Exon 1.0 ST Array – pack of 2 - P/N 900817

·  GeneChip Mouse Exon 1.0 ST Array – pack of 6 - P/N 900818

·  GeneChip Mouse Exon 1.0 ST Array – pack of 30 - P/N 900819

Rat

·  GeneChip Rat Exon 1.0 ST Array – pack of 2 - P/N 900820

·  GeneChip Rat Exon 1.0 ST Array – pack of 6 - P/N 900821

·  GeneChip Rat Exon 1.0 ST Array – pack of 30 - P/N 900822

Labeling reagents

10 reactions kits

·  10 rxns GeneChip® cDNA Synthesis and Amplification Kit (P/N 900672)

·  10 rxns GeneChip® WT Terminal Labeling Kit (P/N 900670)

·  30 rxns GeneChip® IVT cRNA Cleanup Kit (P/N 900547)

·  30 rxns GeneChip® Sample Cleanup Module (P/N 600371)

30 reactions kits

·  30 rxns GeneChip® WT Target Labeling and Control Reagents (P/N 900652)

Or

·  30 rxns GeneChip® Sample Cleanup Module (P/N 600371)

·  30 rxns GeneChip® cDNA Synthesis and Amplification Kit (P/N 900673)

·  30 rxns GeneChip® WT Terminal Labeling Kit (P/N 900671)

·  30 rxns GeneChip® IVT cRNA Cleanup Kit (P/N 900547)

Reagents to order directly from Invitrogen

·  RiboMinus Transcriptome Isolation Kit 6 rxns (P/N K1550-02). This kit provides enough reagents for 40 rxns using our protocol.

·  Magna-Sep Magnetic Particle Separator for 1.5ml tubes (P/N K1585-01).

Reagents to order directly from Sigma

·  Betaine 5M (P/N B-0300), enough for 25 rxns.