Annex 2
Al Rwahnih M, Daubert S, Úrbez-Torres JR, Cordero F, Rowhani A
Deep sequencing evidence from single grapevine plants reveals a virome dominated by mycoviruses.
Archives of Virology
Department of Plant Pathology, University of California, Davis, California
1 Shields Avenue, Davis, CA 95616
Subject Database / The subject database is composed of 105735964 nucleotide sequences.Database / Version / Release Date / Database Status
Genbank - Bacterial division / 178 / Jul 8, 2010 / Most Recent
Genbank - Environmental Samples / 178 / Jul 8, 2010 / Most Recent
Genbank - Expressed Sequence Tags division / 178 / Jul 8, 2010 / Most Recent
Genbank - Genome Survey Sequences division / 178 / Jul 5, 2010 / Most Recent
Genbank - High-throughput cDNA division / 178 / Jul 9, 2010 / Most Recent
Genbank - High-throughput Genomic sequences division / 178 / Jul 9, 2010 / Most Recent
Genbank - Invertebrate division / 178 / Jul 8, 2010 / Most Recent
Genbank - Other Mammalian division / 178 / Jul 9, 2010 / Most Recent
Genbank - Bacteriophage division / 178 / Jul 9, 2010 / Most Recent
Genbank - Plant / fungal / algal division / 178 / Jul 8, 2010 / Most Recent
Genbank - Primate division / 178 / Jul 9, 2010 / Most Recent
Genbank - Rodent division / 178 / Jul 8, 2010 / Most Recent
GB SET / 178 / Jul 9, 2010 / Most Recent
Genbank - Sequence Tagged Sites division / 178 / Jul 9, 2010 / Most Recent
Genbank - Synthetic division / 178 / Jul 8, 2010 / Most Recent
Genbank - Unannotated division / 178 / Jul 9, 2010 / Most Recent
Genbank - Viral division / 178 / Jul 8, 2010 / Most Recent
Genbank - Other Vertebrate division / 178 / Jul 9, 2010 / Most Recent
Search Strategy / The search strategy was Blast with scoring matrix NUC.3.1, an expect cutoff of 10 and word size 11.
Keep the best alignments / For each query, the 1 best alignments were kept.In this case "best alignment" means the alignment with thehighest score.
Alignments Grouped by Organism [top]
Organism #1: Botryotinia fuckeliana
· Organism #1 / Result 1 next | overview
[Q] contig00151 vs [S] AM491608
Query view (% Id) / 65.8Subj. % Id / 2.89
Align % Id / 81.28
E-val / 1.41e-15
Query Identifier / contig00151
Identifier / AM491608
Total View
Description / Botryotinia fuckeliana totivirus 1, complete genome, genomic RNA.
Gene name / CP, RdRp
o See all subjects mapped to this query
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A part of your query matches a part of this sequence.
Align len= 187 nt, Score= 47, Eval= 1.40e-15, Identity= 81.28%, Similarity= 81.28%
Query len= 231 nt, pos= 37-223 nt (fw), Identity query= 65.8%, Alignment coverage query= 80.95%, Nb gaps query= 0
Subject len= 5261 nt, pos= 4903-5089 nt (fw), Identity subject= 2.89%, Alignment coverage subject= 3.55%, Nb gaps subject= 0
Q: 37 GAGGTATTCTAGAGAGCTACCCCCTCATACGGTTACTCGAAAACCGTTTGAGTGATGAAG 96
||||||||||||| ||||| || || || ||||||||||| |||||| |||| || ||||
S: 4903 GAGGTATTCTAGAAAGCTATCCACTTATTCGGTTACTCGAGAACCGTCTGAGCGACGAAG 4962
Q: 97 ACTTGAAGGAGCTTGCCCTTCTTTGTGGAGTGTCATGCTCCACTAAAGAAGCCCGAGTGA 156
|| | ||| || || ||||| |||||| |||||||||||||||||||| ||||
S: 4963 ACCTTCGGGAACTCGCACTTCTCCTTGGAGTATCATGCTCCACTAAAGAAGCGAGAGTCT 5022
Q: 157 TCTGTTTTGGGCCTGAATCACTTTCCCATAACATCATTGGATATTTACCATATAGTGATG 216
||| |||||| |||| |||||| | ||||||||||| || ||| |||| || |||||||
S: 5023 GCTGCTTTGGGTCTGAGTCACTTACTCATAACATCATAGGGTATCTACCCTACAGTGATG 5082
Q: 217 CTGCGTC 223
|||||||
S: 5083 CTGCGTC 5089
· Organism #1 / Result 2 next | previous | overview
[Q] contig00449 vs [S] AM491608
Query view (% Id) / 45.29Subj. % Id / 5.30
Align % Id / 79.94
E-val / 2.98e-28
Query Identifier / contig00449
Identifier / AM491608
Total View
Description / Botryotinia fuckeliana totivirus 1, complete genome, genomic RNA.
Gene name / CP, RdRp
o See all subjects mapped to this query
o See all queries mapped to this subject , [interactive view]
A part of your query matches a part of this sequence.
Align len= 349 nt, Score= 69, Eval= 3.00e-28, Identity= 79.94%, Similarity= 79.94%
Query len= 616 nt, pos= 8-356 nt (rv), Identity query= 45.29%, Alignment coverage query= 56.66%, Nb gaps query= 0
Subject len= 5261 nt, pos= 4063-4411 nt (fw), Identity subject= 5.3%, Alignment coverage subject= 6.63%, Nb gaps subject= 0
Q: 8 TAGGCACACTAGCCAGCGGACACAGAGGGACATCATTCATTAATTCTCTCTGTAATGCCG 67
||||||| || || ||||| ||||||||||||||||||||||| || ||| || || |
S: 4063 TAGGCACGCTGGCAAGCGGGCACAGAGGGACATCATTCATTAACTCCCTCCTGAACGCAG 4122
Q: 68 CGTACATTCGTTGCGCTCTCGGAGCTGCTAGATTCGACTCGATGTTGAGTCTGCACGCTG 127
| |||||||| || ||||| || ||||| | |||||| | ||| |||| ||||||| |
S: 4123 CTTACATTCGGTGTGCTCTTGGGGCTGCAAAGTTCGACACTATGCTGAGCTTGCACGCGG 4182
Q: 128 GTGATGACGTGTATATTAGGGCTAACACTCTTAGTGACGCCGCTGATATACTCAGGAAAT 187
| || ||||| ||||| | || ||||| || ||||| || || ||||| || | |||||
S: 4183 GGGACGACGTATATATCCGCGCCAACACGCTGAGTGAAGCGGCGGATATCCTGAAGAAAT 4242
Q: 188 GTAAGGAGTTTGGCTGTAGGATGAACCCGACGAAACAAAGCATCGGATTCAAGAATGCCG 247
||||| |||||| ||||||||||| || ||||| || || || ||||||| |||||| |
S: 4243 GTAAGATGTTTGGATGTAGGATGAATCCCACGAAGCAGAGTATTGGATTCAGGAATGCAG 4302
Q: 248 AGTTTTTGCGACTCGGAATAAACTCTCGTTACGCTATCGGGTATGTGGCAAGGACCATAT 307
| || || | | ||||| |||||||||||||| | ||||||||||| || |||||||
S: 4303 AATTCTTAAGGTTAGGAATTAACTCTCGTTACGCCGTAGGGTATGTGGCCAGAACCATAT 4362
Q: 308 CTACACTGGTAGCAGGCAATTGGTCTAACCTAGACCCTATGGAACCCCT 356
| || || || ||||| |||||||||||||||||||| |||||||||||
S: 4363 CGACCCTAGTGGCAGGAAATTGGTCTAACCTAGACCCCATGGAACCCCT 4411
· Organism #1 / Result 3 next | previous | overview
[Q] contig00462 vs [S] AM491608
Query view (% Id) / 39.97Subj. % Id / 6.06
Align % Id / 78.19
E-val / 5.44e-18
Query Identifier / contig00462
Identifier / AM491608
Total View
Description / Botryotinia fuckeliana totivirus 1, complete genome, genomic RNA.
Gene name / CP, RdRp
o See all subjects mapped to this query
o See all queries mapped to this subject , [interactive view]
A part of your query matches a part of this sequence.
Align len= 408 nt, Score= 52, Eval= 5.40e-18, Identity= 78.19%, Similarity= 78.19%
Query len= 798 nt, pos= 46-453 nt (rv), Identity query= 39.97%, Alignment coverage query= 51.13%, Nb gaps query= 0
Subject len= 5261 nt, pos= 1528-1935 nt (fw), Identity subject= 6.06%, Alignment coverage subject= 7.76%, Nb gaps subject= 0
Q: 46 ACTGCTCTGGATGTCGACAACCGGCACCTCCGACACGCATCGGTGTCACCGTGGTTCTGG 105
||||| ||||| || |||||||||||||| ||||| || ||||| || |||||||||
S: 1528 ACTGCGCTGGACGTGGACAACCGGCACCTTAAGCACGCGTCAGTGTCTCCTTGGTTCTGG 1587
Q: 106 ATAGAACCGACAAGTATCTTACCGCCGAACCTACTCGGTTCAGAGGCTGAGATCGAAGGT 165
| |||||||| || ||| |||| || |||||||| || || || |||||| | || ||
S: 1588 GTGGAACCGACCAGCATCCTACCCCCCAACCTACTTGGGTCGGAAGCTGAGCTAGAGGGG 1647
Q: 166 TGCGCCTCCTTGGCGTATAAAGATACTGTCGTGTCCAAACCCGCGTGGGATACGATAACT 225
|| || || |||| | |||||||| || || || ||||| || ||||| || || ||
S: 1648 TGTGCATCACTGGCACACAAAGATACGGTGGTCTCAAAACCAGCCTGGGACACCATTACC 1707
Q: 226 AGGGTGGGCTCAAGTGACGTGTCTTTTTCTGCGTATCGTGCTCGTTTTAAATTCGCACGA 285
|||||||| || || |||||||| ||||| |||||||| || || || || || || ||
S: 1708 AGGGTGGGTTCGAGCGACGTGTCCTTTTCCGCGTATCGCGCGCGATTCAAGTTTGCCCGC 1767
Q: 286 CAAACGTGGTTTTTCAACCACTGGCTTAACCACCCCATGAATGGCCTCGGGGCTATGAAG 345
||||| ||||| || ||||| ||||| |||||||| ||||| || ||||| |||||||||
S: 1768 CAAACTTGGTTCTTTAACCATTGGCTCAACCACCCAATGAACGGGCTCGGTGCTATGAAG 1827
Q: 346 GTCAGACAACTCGATCCCAATGGTATCATACACCCTGGGCCCTGTGCCGCACATCCTCAA 405
|| |||| || || || ||||||||||| || || ||||||||| | || || || ||
S: 1828 GTACGACAGCTTGACCCAAATGGTATCATCCATCCGGGGCCCTGTACGGCCCACCCGCAG 1887
Q: 406 GTGCGAGACCGTGTCGAAGCTGACCTTCCGTTCACGGATTACCTCTGG 453
||| ||||||| ||||| || ||||| || || || || |||||||||
S: 1888 GTGAGAGACCGGGTCGAGGCGGACCTACCTTTTACTGACTACCTCTGG 1935
· Organism #1 / Result 4 next | previous | overview
[Q] contig00250 vs [S] AM491608
Query view (% Id) / 38.96Subj. % Id / 5.00
Align % Id / 79.22
E-val / 1.88e-20
Query Identifier / contig00250
Identifier / AM491608
Total View
Description / Botryotinia fuckeliana totivirus 1, complete genome, genomic RNA.
Gene name / CP, RdRp
o See all subjects mapped to this query
o See all queries mapped to this subject , [interactive view]
A part of your query matches a part of this sequence.
Align len= 332 nt, Score= 56, Eval= 1.90e-20, Identity= 79.22%, Similarity= 79.22%
Query len= 675 nt, pos= 327-658 nt (rv), Identity query= 38.96%, Alignment coverage query= 49.19%, Nb gaps query= 0
Subject len= 5261 nt, pos= 3624-3955 nt (fw), Identity subject= 5%, Alignment coverage subject= 6.31%, Nb gaps subject= 0
Q: 327 TACAGACGGATGGCTGCCGAGGAGGTCCACATCAACCCCGTACCCTCATGGGACGGTACC 386
|||||||| |||||||| |||||||| |||| |||||| ||||| |||||||| ||
S: 3624 TACAGACGCATGGCTGCAGAGGAGGTACACAGCAACCCAGTACCAGACTGGGACGGCACA 3683
Q: 387 ACTTCTGTCTCGGCAAGCATCAAACTAGAAGCAGGCAAAGATCGAGCTATCTTCGCCTGT 446
||||| || || || || ||||| || || ||||| || ||||| || |||||||| ||
S: 3684 ACTTCGGTATCAGCGAGTATCAAGCTCGAGGCAGGTAAGGATCGCGCAATCTTCGCTTGC 3743
Q: 447 GATACTAGGTCTTACTTCGCTTTTTCCTGGATACTCAATGACGTCCAAAAGAGGTGGAGG 506
|| || | ||||||||||| || || ||||||||||||||||| ||||||| ||||| |
S: 3744 GACACCCGTTCTTACTTCGCGTTCTCGTGGATACTCAATGACGTGCAAAAGAAGTGGAAG 3803
Q: 507 GGTGAGCGTATACTGCTGGATCCGGGGAAGGGTGGTTTGTACGGTATCTCGCGTAGGATA 566
|| ||| | || | ||||||| ||||| || || ||||||||||||| || | ||
S: 3804 GGGGAGAGGATCCCTTTGGATCCAGGGAAAGGCGGACTGTACGGTATCTCACGCCGAATC 3863
Q: 567 CGCAACTGTCAGAAGGGCGGTGGCGTCAACCTAATGCTCGACTACGACAATTTCAACTCT 626
|| ||| |||||| || ||||| ||||| | ||||| ||||||||||| || |||||
S: 3864 CGTAACAGTCAGAGAGGAGGTGGAGTCAATTTGATGCTTGACTACGACAACTTTAACTCG 3923
Q: 627 CATCACTCTAACTCTGTCCAGAGTATGATCTT 658
|||||||| ||| |||||||||||||||||
S: 3924 CATCACTCCAACAGCGTCCAGAGTATGATCTT 3955
· Organism #1 / Result 5 next | previous | overview
[Q] contig00473 vs [S] AM491608
Query view (% Id) / 31.73Subj. % Id / 2.76
Align % Id / 79.67
E-val / 1.68e-7
Query Identifier / contig00473
Identifier / AM491608
Total View
Description / Botryotinia fuckeliana totivirus 1, complete genome, genomic RNA.
Gene name / CP, RdRp
o See all subjects mapped to this query
o See all queries mapped to this subject , [interactive view]
A part of your query matches a part of this sequence.
Align len= 182 nt, Score= 34, Eval= 1.70e-7, Identity= 79.67%, Similarity= 79.67%
Query len= 457 nt, pos= 2-183 nt (rv), Identity query= 31.73%, Alignment coverage query= 39.82%, Nb gaps query= 0
Subject len= 5261 nt, pos= 1690-1871 nt (fw), Identity subject= 2.76%, Alignment coverage subject= 3.46%, Nb gaps subject= 0
Q: 2 GCCTGGGATACTATAACTAGGGTCGGGTCCAGCGACGTTTCTTTCTCTGCGTACCGTGCG 61
|||||||| || || || ||||| || || |||||||| || || || ||||| || |||
S: 1690 GCCTGGGACACCATTACCAGGGTGGGTTCGAGCGACGTGTCCTTTTCCGCGTATCGCGCG 1749
Q: 62 CGTTTCAAGTTCGCGAGGCAGACTTGGTTCTTCAATCATTGGTTGAACCACCCGATGAAC 121
|| |||||||| || | || ||||||||||| || |||||| | |||||||| ||||||
S: 1750 CGATTCAAGTTTGCCCGCCAAACTTGGTTCTTTAACCATTGGCTCAACCACCCAATGAAC 1809
Q: 122 GGTTTAGGTGCGATGAAGGTGCGGCAGCTCGATCCGAACGGCATCATACACCCGGGGCCC 181
|| | ||||| |||||||| || ||||| || || || || ||||| || |||||||||
S: 1810 GGGCTCGGTGCTATGAAGGTACGACAGCTTGACCCAAATGGTATCATCCATCCGGGGCCC 1869
Q: 182 TG 183
||
S: 1870 TG 1871
· Organism #1 / Result 6 next | previous | overview
[Q] contig00271 vs [S] AM491608
Query view (% Id) / 30.25Subj. % Id / 1.62
Align % Id / 81.73
E-val / 3.82e-4
Query Identifier / contig00271
Identifier / AM491608
Total View
Description / Botryotinia fuckeliana totivirus 1, complete genome, genomic RNA.
Gene name / CP, RdRp
o See all subjects mapped to this query
o See all queries mapped to this subject , [interactive view]
A part of your query matches a part of this sequence.
Align len= 104 nt, Score= 28, Eval= 3.80e-4, Identity= 81.73%, Similarity= 81.73%
Query len= 281 nt, pos= 166-269 nt (fw), Identity query= 30.25%, Alignment coverage query= 37.01%, Nb gaps query= 0
Subject len= 5261 nt, pos= 3624-3727 nt (fw), Identity subject= 1.62%, Alignment coverage subject= 1.98%, Nb gaps subject= 0
Q: 166 TACAGACGGATGGCTGCCGAGGAGGTCCATGTCAACCCTGTTCCATCATGGGATGGTACA 225
|||||||| |||||||| |||||||| || |||||| || ||| ||||| || |||
S: 3624 TACAGACGCATGGCTGCAGAGGAGGTACACAGCAACCCAGTACCAGACTGGGACGGCACA 3683
Q: 226 ACCTCTGTCTCAGCCAGTGTCAAGCTCGAAGCAGGTAAGGATCG 269
|| || || ||||| ||| |||||||||| ||||||||||||||
S: 3684 ACTTCGGTATCAGCGAGTATCAAGCTCGAGGCAGGTAAGGATCG 3727
· Organism #1 / Result 7 next | previous | overview
[Q] contig00720 vs [S] AM491608
Query view (% Id) / 29.4Subj. % Id / 2.51
Align % Id / 83.54
E-val / 1.92e-19
Query Identifier / contig00720
Identifier / AM491608
Total View
Description / Botryotinia fuckeliana totivirus 1, complete genome, genomic RNA.
Gene name / CP, RdRp
o See all subjects mapped to this query
o See all queries mapped to this subject , [interactive view]
A part of your query matches a part of this sequence.
Align len= 158 nt, Score= 54, Eval= 1.90e-19, Identity= 83.54%, Similarity= 83.54%
Query len= 449 nt, pos= 291-448 nt (rv), Identity query= 29.4%, Alignment coverage query= 35.19%, Nb gaps query= 0
Subject len= 5261 nt, pos= 4251-4408 nt (fw), Identity subject= 2.51%, Alignment coverage subject= 3%, Nb gaps subject= 0
Q: 291 TTTGGTTGTAGGATGAATCCAACAAAGCAAAGCATCGGATTCAGGAATGCCGAGTTCTTA 350
||||| |||||||||||||| || ||||| || || |||||||||||||| || ||||||
S: 4251 TTTGGATGTAGGATGAATCCCACGAAGCAGAGTATTGGATTCAGGAATGCAGAATTCTTA 4310
Q: 351 CGTCTCGGAATCAACTCTCGTTACGCTATCGGTTATGTCGCCAGGACAATATCCACACTG 410
| | ||||| |||||||||||||| | || ||||| ||||| || ||||| || ||
S: 4311 AGGTTAGGAATTAACTCTCGTTACGCCGTAGGGTATGTGGCCAGAACCATATCGACCCTA 4370
Q: 411 GTAGCAGGCAATTGGTCTAACCTAGACCCAATGGAACC 448
|| ||||| |||||||||||||||||||| ||||||||
S: 4371 GTGGCAGGAAATTGGTCTAACCTAGACCCCATGGAACC 4408
· Organism #1 / Result 8 next | previous | overview
[Q] contig00626 vs [S] AM491608
Query view (% Id) / 19.48Subj. % Id / 2.70
Align % Id / 80.68
E-val / 7.18e-11
Query Identifier / contig00626
Identifier / AM491608
Total View
Description / Botryotinia fuckeliana totivirus 1, complete genome, genomic RNA.
Gene name / CP, RdRp
o See all subjects mapped to this query
o See all queries mapped to this subject , [interactive view]
A part of your query matches a part of this sequence.
Align len= 176 nt, Score= 40, Eval= 7.20e-11, Identity= 80.68%, Similarity= 80.68%
Query len= 729 nt, pos= 83-258 nt (fw), Identity query= 19.48%, Alignment coverage query= 24.14%, Nb gaps query= 0
Subject len= 5261 nt, pos= 775-950 nt (fw), Identity subject= 2.7%, Alignment coverage subject= 3.35%, Nb gaps subject= 0
Q: 83 GGCACTTACGACGGGCCAGTCAACTCTTTGACTGATGCCGTCTACATACCGAGGTTGGTG 142
||||| || ||||| |||||||| || ||||||| ||||| |||||||| ||| ||||
S: 775 GGCACCTATGACGGCCCAGTCAATTCGCTGACTGACGCCGTGTACATACCAAGGCTGGTA 834
Q: 143 AATACCACCTTGACCGGCGATGTGTTCGCCGTCCTTTGTAACGCCGTGGCTGGTGAGGGA 202
|| ||||||||||| || || || ||||| || | ||||||||||| |||||||||||
S: 835 AACACCACCTTGACAGGTGACGTCTTCGCGGTGTTATGTAACGCCGTTGCTGGTGAGGGC 894
Q: 203 TCACAAGTGGTCTCTGATGTTGTCGAGCTTGATGCGAACACGCGTCAACCTATAAT 258
|| || || || ||||| || || ||||| ||||| ||||| || ||||| |||||
S: 895 TCGCAGGTCGTTTCTGACGTCGTTGAGCTCGATGCCAACACCCGCCAACCCATAAT 950
· Organism #1 / Result 9 next | previous | overview
[Q] contig00510 vs [S] AM491608
Query view (% Id) / 13.71Subj. % Id / 1.62
Align % Id / 84.16
E-val / 3.75e-9
Query Identifier / contig00510
Identifier / AM491608
Total View
Description / Botryotinia fuckeliana totivirus 1, complete genome, genomic RNA.
Gene name / CP, RdRp
o See all subjects mapped to this query
o See all queries mapped to this subject , [interactive view]
A part of your query matches a part of this sequence.
Align len= 101 nt, Score= 37, Eval= 3.70e-9, Identity= 84.16%, Similarity= 84.16%
Query len= 620 nt, pos= 140-240 nt (rv), Identity query= 13.71%, Alignment coverage query= 16.29%, Nb gaps query= 0
Subject len= 5261 nt, pos= 2970-3070 nt (fw), Identity subject= 1.62%, Alignment coverage subject= 1.92%, Nb gaps subject= 0
Q: 140 AGGAGGGGGTATCACCGGAAGTTGGGTAATGCCATCATTACCAATACCGAGCTAAGGGAC 199
|||||||| || ||||| ||| | ||||| || || |||||||| | ||||| ||||||
S: 2970 AGGAGGGGCTACCACCGAAAGCTAGGTAACGCTATAATTACCAACAAGGAGCTTAGGGAC 3029
Q: 200 TCACTCTTCCCTAAGAAGAAACACAACGCAGCAGGCGTTAA 240
|| |||||||||||||||||||| |||||||| || |||||
S: 3030 TCCCTCTTCCCTAAGAAGAAACATAACGCAGCCGGTGTTAA 3070