ISOLATION of CHEMICAL CONSTITUNTS and PHRMACOLOGICAL STUDYOF Tylophora Indica

ISOLATION OF CHEMICAL CONSTITUNTS AND PHRMACOLOGICAL STUDYOF Tylophora Indica

PROTOCOL FOR

M.PHARM DISSERTATION

SUBMITTED TO THE

RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCES,

KARNATAKA, BANGALORE

SUHAS BABANRAO GURAV, B.Pharm.

DEPARTMENT OF PHARMACEUTICAL CHEMISTRY,

2010-2011.

UNDER THE GUIDANCE OF

Mr. DEV PRAKASH,M.Pharm, (Ph.D), F.A.G.E

ASSISTANT PROFESSOR,

DEPARTMENT OF PHARMACEUTICAL CHEMISTRY,

BHARATHI COLLEGE OF PHARMACY,

BHARATHI NAGARA,

KARNATAKA- 571422.

RAJIV GANDHI UNIVERSITY OF HEALTH SCIENCE, KARNATAKA, BANGALORE.

ANNEXURE-II

PROFORMA FOR REGISTRATION OF SUBJECTS FOR DISSERTATION

1. / Name of the Candidate and Address (In Block Letters) / PRESENT ADDRESS:
SUHAS BABANRAO GURAV,
I M. Pharm.,
DEPARTMENT OF PHARMACEUTICAL CHEMISTRY,
C/O BHARATHICOLLEGE OF PHARMACY,
BHARATHI NAGARA,
MANDYA (DIST),
KARNATAKA-571422.
2. / Name of the Institution / BHARATHICOLLEGE OF PHARMACY
BHARATHI NAGARA, MANDYA- 571422.
3. / Course of Study and Subject / MASTER OF PHARMACY IN PHARMACEUTICAL CHEMISTRY
4. / Date of Admission of Course / 18/3/ 2010
5. / Title of Topic / ISOLATION OF CHEMICAL CONSTITUNTS AND PHRMACOLOGICAL STUDY
OF Tylophora Indica
6. / Brief Resume of the Intended Work
6.1 Need for the study
6.2 Review of the literature
6.3 Objectives of the study / ENCLOSURE-I
7 / Materials and Methods
7.1 Source of data
7.2 Method of collection of data
7.3 Does study require any investigations or interventions to conduct on patients or other human or animal? If so, please describe briefly.
7.4 Has ethical clearance been obtained from your institution in case of 7.3 / ENCLOSURE-II
8 / List of References / ENCLOSURE-III
9 / Signature of Candidate / (SUHAS BABANRAO GURAV)
10 / Remarks of Guide / This work will carry out in college laboratory
11 / Name and Designation of
11.1 Guide
11.2 Signature / Mr. DEV PRAKASH ,
ASSISTANT PROFESSOR,
DEPARTMENT OF
PHARMACEUTICALCHEMISTRY,
BHARATHICOLLEGE OF PHARMACY,
BHARATHI NAGARA,
KARNATAKA-571422.
11.3 Co- Guide (if any)
11.4 Signature / NOT APPLICABLE.
11.5 Head of Department
11.6 Signature / Dr. SENTHIL KUMAR. G.P.,
PROFESSOR AND HEAD,
DEPARTMENT OF PHARMACEUTICAL CHEMISTRY,
BHARATHICOLLEGE OF PHARMACY,
BHARATHI NAGARA,
KARNATAKA-571422.
12 / 12.1 Remarks of the Chairman and Principal
Forwarded for Approval
12.2Signature / Dr. T. TAMIZH MANI, PRINCIPAL, BHARATHI COLLEGE OF PHARMACY, BHARATHI NAGARA, KARNATAKA -571422.

ENCLOSURE-I

6. BRIEF RESUME OF THE INTENDED WORK

6.1 NEED FOR THE STUDY:

In developing world, the trend and utility of natural product medicinal substances is increased in last decade over synthetic medicine, because of toxic effect. WHO currently recommended and encourages traditional herbal remedies in National Health Care Programs, because herbal drug easily available at low cost and comparatively safe. Plants are source of many medicines.

Tylophora indica is a medium sized plant belonging to family Asclepidaceae. It is found in southern and eastern part of India. From the literature survey, preliminary investigation shows alkaloids, glycosides, carbohydrates, saponin, tannins, protein and amino acids1,2,5.

The plant has been reported to contain 0.2-0.46% alkaloids viz. Tylophorine,tylophorinine, tylophorinidine,septicine, isotylocrebrine,tylophorinicine, sterols, flavanoids, wax,resins, and tannins11,2,4. The plant has beentraditionally used for the treatment ofbronchial asthma, jaundice andinflammation1,2. Antitumor14, anticancer13,immunomodulatory12, antiasthmatic15, smooth muscle relaxant6,and hypotensive9 activity of this plant was scientifically proven.

It was found that no substantial work of Tylophora indica will carried out for its Anti-inflammatory, antioxidant, nephroprotective activities4. Hence, the present work to study these activities of various extract ofTylophora indica and to isolate the chemical constituent.

6.2REVIEW OF THE LITERATURE:-

Mayank Guptaet al.,4 studies on Phyto-pharmacological and plant tissue culture overview of Tylophora indica (burm f.) Merill. It is said to have laxative, expectorant, diaphoretic and purgative properties. It has been used for the treatment of various respiratory problems besides asthma including allergies, bronchitis and colds as well as dysentery and osteoarthritis pain. The plant has been reported to contain 0.2-0.46% alkaloids, flavanoids, wax, resins, and tannins,etc.
Meera et al.,5studies on Aqueous and alcoholic extracts of Tylophora indica leaves were tested for diuretic activity in rats. Theparameters studied on individual rat were body weight before and after test period, total urine volume,urine concentration of Na+, K- and Cl-. It showed increase in urine volume, cation and anion excretion.
Narasimhaet al.,6 studies on Antifeedent and antimicrobial activity of crude and pure extract of Tylophora indica. Tylophorine showed the highest antifeedant activity. The crude extracts of leaf have exhibited higher antibacterial activity than root and shoot against Basillus subtilis, Staphylococcus aureus, Mycrococcus luteus and P.aergenosa.
Mujeebet al.,7studies Hepatoprotective activity of the methanolic extract of Tylophora indica leaves. This plant screened for hepatoprotective activity in carbon tetrachloride induced hepatotoxicity in albino rats.
Nayampalliet al.,8 studies on Evaluation of anti-allergic activity of Tylophora indica using rat lung perfusion. This article show, the anti-allergic effect of Tylophora indica was compared with that of disodium cromoglycate on perfused rat lung in sensitized rats by observing the changes in the volume of the perfusate per minute. Administration of aqueous extract of Tylophora indica and disodium cromoglycate during perfusion of sensitized rat lung significantly increased the rate of flow.
Dhananjayanet al.,9 studies on the pharmacological effect such as myocardial depression, smooth muscle relaxant, etc., of extract and total alkaloids of Tylophora indica.
Gupta Mayanket al., 10 studies on HPTLC Fingerprinting of different leaf extracts of Tylophora indica. A method has been developed for different extracts of Tylophora indica for HPTLC fingerprinting analysis for identification and quantification of marker compound.
Venkat N Rao et al.,11 studies on Hepatoprotective activity of alcoholic and aqueous extracts of leaves of Tylophora indica (Linn.) in rats. They investigate the hepatoprotective activity against ethanol-induced hepatotoxicity. The alcoholic extract was found to exhibit greater hepatoprotective activity than the aqueous extract.
Ganguly et al., 12 studies on Immunomodulatory effect of Tylophoraindica on Con A induced lymph proliferation.
Chia-Maoet al., 13 studies on anticancer activity.The Tylophorine arrests carcinoma cells at G1 phase by downregulating cyclin A2 expression.

6.3 OBJECTIVES OF THE STUDY:

Phytochemical and pharmacological study of Tylophora indica by

1) Authentification and collection of plant

2) Extraction with various solvent.

3) Isolation of phytoconstituents from extracts.

4) Evaluate Anti-inflammatory, antioxidant, nephroprotective activities studies of extracts.

ENCLOSURE-II

7. METHODOLOGY:

7.1 SOURCE OF DATA:

The literature survey will be done by referring abstracts of the many national and international journals.
The information about pharmacological and biological activity has be collected by referring International Journal of Green Pharmacy, Journal of Pharmaceutical Sciences and Research, Journal of Pharmaceutical Sciences, Indian Journal of Pharmacology.
Library of Bharathi college of Pharmacy, Bharthinagara.
The day to day development in this area will be updated by literature survey through E-publishing and current periodical in library of Bharathi college of Pharmacy, Bharthinagara.
All the basic facilities required for pharmacological activity are available in the laboratories of Bharathi college of Pharmacy, Bharthinagara.