Sample Preparation Laboratories

User Access Agreement

October 2017

Welcome to the SSRL and LCLS sample preparation laboratories. Due to safety concerns for all laboratory users, experimental samples and our equipment, the laboratories have restricted access. Each group member mustcomplete laboratory training and submit the attachedform to access the laboratories. This User Access Agreement form must be completed every time your visitrequires laboratory space. The group will be assigned bench space and granted access to the laboratory via access key codeor RFID badge. You cannotdistribute the codeor provide lab access to other individuals.Detailed Sample Preparation Laboratory information is available online at

Primary Contacts

The primary contacts for all Sample Preparation Laboratory resources and mattersare Cynthia Patty (SSRL) and Kayla Zimmerman (LCLS).

Facility / Name / E-mail / Telephone / Office
SSRL / Cynthia Patty / / Office: 650-926-3925
Cell: 925-381-9299 / B131 Rm211
LCLS / Kayla Zimmerman / / Office: 650-926-6281
Cell: 951-249-1066 / B950 Rm219
SSRL back up / Risa Benwell / / Office: 650-926-6283
Cell: 573-268-6953 / B120 Rm200

Other Important Contacts

  • SSRL Safety: Matt Padilla (x3861), Ian Evans (x2628)
  • LCLS Safety: Ian Evans (x2628)
  • SSRL AFTER HOURS contact:SSRL Duty Operator (x4040)
  • LCLS AFTER HOURS contact: Duty Technicians (x6267) & experimental Point Of Contact
  • Non-emergency: SLAC Medical Department (x2281) or SLAC Security (x5555)
  • Emergency:911 or x5555

Responsible Parties

The SpokespersonorLead Person for each group must ensure that each group membercompletes lab training and any applicable equipment training. The spokesperson takes ultimate responsibility for the entire group’s proper use of the laboratory, including strict adherence to laboratory rules and approved Standard Operating Procedures (SOPs)

Each group member is responsible for completing necessary training, using appropriate personal protection equipment (PPE), properly using laboratory resources, and cleaning up their assigned area and hazardous wastes. Each group member is responsible for strict adherence to laboratory rules and approved Standard Operating Procedures (SOPs).

Safety glasses are required to enter the laboratory, and nitrile/latexgloves are required for all chemical and sample handling. The door to the sample prep labmust be kept closed apart from access.

Laboratory Training

Each group member must complete Course 204, Sample PreparationLaboratory training annually. Course 204 is offered at SSRL and LCLS. SSRL sessions are Monday–Friday at 10am 3pm and meet in B131 Rm 211; drop ins are welcome but not guaranteed. LCLS sessions are by appointment only and meet in B999 Rm 109. To make an appoinment please contact your experimental POC and Kayla Zimmerman .LCLS training slots must be reserved in advance due to space limitations within the LCLS labs. Weekend training is not available.

Your Work Area & Chemicals

Each group must work within its assigned workspace.All chemical containers must be labeled with chemical composition, owner’s name, date, & beam line or extension. Unlabeled chemicals, solutions, or samples may be considered unsafe, and therefore discarded.

All work involving acutely toxic and/or volatile materials isperformed in fume hoods.

Work with any unsealed samples or reagents that are significantly toxic, reactive, corrosive, flammable, or otherwise especially hazardous, may require an approved SOP before work commences. Examples: Reagents with NFPA ratings of 3 or 4, heavy metals, pyrophoric materials, water reactive materials. Each group member is responsible for strict adherence to approved SOPs.

Chemicals and samples cannot be stored in the laboratory after the group’s beam time is complete. The group is required to cleanall workspaces, glassware, analytical balances,and equipment used.

SSRL Glove Boxes & Ultracentrifuges

Use of a dryglove box requires training. Glove Box training is available by appointment. An environmental anaerobic chamber is also available with permission. Use of a glove box, anaerobic chamber, or ultracentrifuge must be registered in the instrumental log book.

Borrowing

Equipment, supplies, and chemicals should not be removed from the laboratory, moved to other laboratories, or relocated to beam lines.

Specialty supplies (micropipettes, agate mortar & pestles, PTFE tools, etc.) are available on a check-out basis. A complete list is available here:

Each user assumes responsibility for exercising exemplary care in the use of the equipment. The cost for damage or malfunction caused by negligence will be billed to the user's home institution.

Hazardous Waste

Detailed hazardous waste disposal guidelines are posted in each laboratory. All hazardous wastes are segregated by hazard class and stored in the Hazardous Waste cabinetwithin each laboratory. Hazardous wastes must be contained appropriately, and labeled with chemical composition, hazards, contact person, beam line, and date. Glass & plastic containers and labels are provided in the laboratories.Transfer of toxic or volatile materials to waste containers must occur in a fume hood. Empty reagent bottles should also be treated as hazardous waste.

Solvent Waste Accumulation (SWA) bottles are available in the Hazardous Waste cabinets for disposal of organic solvents. SWA bottles are labeled according to solvent type, and disposal must be made accordingly.Aqueous, corrosive, or reactive chemicals must never be deposited in SWA bottles.

Sharps & Glassware Disposal

Non-hazardous damaged glassware and broken glass are put in the Broken Glassware Disposalcontainer. Disposable pipets and capillary tubes must be cleaned prior to disposal. If damaged glassware is contaminated with hazardous material, please follow guidelines for hazardous waste. Empty reagent bottles must also be treated as hazardous waste. Used syringe needlesand other sharp items are rinsed and disposed of in Sharps containers. Glassware or needles must never be thrown in the trash bin.

Sample Preparation Laboratory User Agreement, C. Patty 10/25/2017

Sample Preparation Laboratory Responsibility Form

I hereby take responsibility for the use of the Sample Preparation Laboratory by myself and my group, for which I am the Spokesperson, according to the rules outlined above and within the laboratory training. Each member of my group (listed below) has current laboratory training and understands the rules and responsibilities therein.

Spokesperson (printed) …………………………………….………… / Date ……………………………….….
Signature ……………….……………………………………………… / Beam line ………………..………….
Institution …………………………………………………………………………………….……………….………

If the Spokesperson is unavailable, please indicate a Lead Person in her/his place, below. The Lead Person will take responsibility for the entire group’s activities on behalf of the Spokesperson.

I hereby take responsibility for the use of the Sample Preparation Laboratory by myself and my group, for which I am the Lead Person, according to the rules outlined above and within the laboratory training. Each member of my group (listed below) has current laboratory training and understands the rules and responsibilities therein.

Lead Person (printed) ……………………………………………….… / Date ……………………………….….
Signature ……………….…………………………………………..…… / Beam line …………………..……….

Please have any other members of your group sign below.

I hereby take responsibility for my use of the Sample Preparation Laboratory according to the rules outlined above and within the laboratory training. I have current laboratory training and understand the rules and responsibilities therein.

Group Member (printed) …….…………………… / Signature …………………………. / Date ……………
Group Member (printed) ………….……………… / Signature …………………………. / Date ……………
Group Member (printed) ………….……………… / Signature …………………………. / Date ……………
Group Member (printed) ………….……………… / Signature …………………….…… / Date ……….…..
Group Member (printed) …………………….…… / Signature ……………………...….. / Date ……………
Group Member (printed) …………………….…… / Signature ……………………...….. / Date ……………

Sample Preparation Laboratory User Agreement, C. Patty 10/25/2017

Sample Preparation Laboratory Space Request Form

Proposal #: / Spokesperson: / Lead Person:
Beam Line: / Start date: / End date:
Lab Room Assignment:

nitialsstood? s) are understood. use an aproved longer exists.edures (SOPs)y to Stanford Chemical Compatibility sytem . Please indicate below if your approved Standard Operating Procedures (SOPs) will be used. All SPL experimenters must initial below to indicate that they have read and understood their SOP(s) and SOP parameters will be strictly followed.

SOP(s) in use: / Participants (initial):

Please list your chemicals and samples below.

Describe Chemical/Sample Composition: / Quantity (mass or volume): / pH: / Hazards: / Nanomaterial Particle Size:

Sample Preparation Laboratory User Agreement, C. Patty 10/25/2017