Archives of Microbiology Online Resource 5
Genome mining of lipolytic exoenzymes from Bacillus safensis S9 and Pseudomonas alcaliphila ED1, isolated from a dairy wastewater lagoon.
Florencia Ficarraa,c, Ignacio Santecchiaa,c, Sebastián Lagorioc, Sergio Alarcónb,c, Christian Magnia,c, Martín Espariza,c*
a Instituto de Biología Molecular de Rosario (IBR-CONICET), Suipacha 531, (S2002LRK) Rosario, Argentina.
b Instituto de Química de Rosario (IQUIR-CONICET), Suipacha 531, (S2002LRK) Rosario, Argentina.
c Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Suipacha 531, (S2002LRK) Rosario, Argentina.
Putative biotechnological relevant enzymes encoded in B. safensis S9 and P. alcaliphila ED1Strain / Protein_name / Sequence* / Length (AA) / Description
Contig or scaffold number / Start pos. / End position
S9 / Acetolactate decarboxylase_S9_3056 / 11 / 42704 / 41937 / 256 / Alpha-acetolactate decarboxylase (EC 4.1.1.5)
Azoreductase_S9_2470 / 25 / 157648 / 157016 / 211 / FMN-dependent NADH-azoreductase
Azoreductase_S9_2732 / 11 / 372479 / 371838 / 214 / FMN-dependent NADH-azoreductase
Catalase_S9_1880 / 8 / 369621 / 370448 / 276 / Catalase (EC 1.11.1.6)
Catalase_S9_3511 / 49 / 1507 / 3093 / 529 / Catalase (EC 1.11.1.6)
Catalase_S9_394 / 17 / 66704 / 65232 / 491 / Catalase (EC 1.11.1.6)
Catalase_S9_816 / 17 / 455745 / 456638 / 298 / Manganese catalase (EC 1.11.1.6)
Glucanase_S9_1073 / 17 / 702121 / 703968 / 616 / Endoglucanase 4 precursor (EC 3.2.1.4)
Glucanase_S9_1074 / 17 / 704004 / 706106 / 701 / Exoglucanase II precursor (EC 3.2.1.91)
Glucanase_S9_2386 / 12 / 31568 / 32293 / 242 / Endo-beta-1,3-1,4 glucanase (Licheninase) (EC 3.2.1.73)
Glucosyltransferase_S9_3013 / 11 / 88892 / 90355 / 488 / Poly(glycerol-phosphate) alpha-glucosyltransferase (EC 2.4.1.52)
Pectate lyase_S9_3303 / 38 / 41725 / 42732 / 336 / pectate lyase( EC:4.2.2.2 )
Xylanase_S9_1275 / 17 / 898767 / 897241 / 509 / Endo-1,4-beta-xylanase A precursor (EC 3.2.1.8)
Xylanase_S9_1354 / 6 / 27572 / 26889 / 228 / xylanase
ED1 / Amylase_ED1_23 / 14.1 / 33165 / 31186 / 660 / Alpha-amylase (EC 3.2.1.1)
Azoreductase_ED1_1176 / 5.1 / 57957 / 58655 / 233 / FMN-dependent NADH-Azoreductase
Azoreductase_ED1_1434 / 1.1 / 6746 / 6141 / 202 / FMN-dependent NADH-Azoreductase
Azoreductase_ED1_1439 / 1.1 / 13363 / 13956 / 198 / Azoreductase
Azoreductase_ED1_1586 / 1.1 / 176782 / 177396 / 205 / FMN-dependent NADH-Azoreductase
Azoreductase_ED1_2931 / 1.1 / 1649530 / 1650126 / 199 / FMN-dependent NADH-Azoreductase
Catalase_ED1_3 / 14.1 / 3320 / 1185 / 712 / Catalase (EC 1.11.1.6)
Catalase_ED1_4065 / 3.1 / 353981 / 351834 / 716 / Catalase (EC 1.11.1.6) / Peroxidase (EC 1.11.1.7)
Catalase_ED1_4662 / 9.1 / 33458 / 34894 / 479 / Catalase (EC 1.11.1.6)
Catalase_ED1_681 / 2.1 / 705986 / 704889 / 366 / Catalase (EC 1.11.1.6)
Glucosyltransferase_ED1_1261 / 5.1 / 141436 / 138899 / 846 / Glucans biosynthesis glucosyltransferase H (EC 2.4.1.-)
Glucosyltransferase_ED1_2205 / 1.1 / 844604 / 845725 / 374 / UDP-glucose:(heptosyl) LPS alpha1,3-glucosyltransferase WaaG (EC 2.4.1.-)
CDS of B. safensis S9 and P. alcaliphila ED1 were identified and assigned using RAST (Aziz et al., 2008). * Data correspond to sequences LIHF01000000 and LLXP01000000, respectively.