Supplemental Table S1. Cluster fresh weight, leaf area and leaf/cluster ratio of Cabernet Sauvignon fruiting cuttings with different two, six, and twelve leaves per cluster.Asterisks denote significant differences (*P<0.05)
Cluster weight(g) / Leaf area(m2) / Leaf/cluster ratio (m2/Kg)2L / 82,51 ± 8,18 / 0,0356 ± 0,0105 / 0,4363 ± 0,1425
6L / 113,90 ± 15,78 / 0,0710 ± 0,0066 / 0,6375 ± 0,1554
12L / 100,56 ± 17,66 / 0,1556 ± 0,0342* / 1,6249 ± 0,6728*
Supplemental Table S2. Primers used for in real-time PCR studies.
Primer forward(5' - 3') / Primer reverse
(5' - 3') / Accession number (12x)
VvGPT1 / TCAATGCTCTTGGAGCTGCCATC / CTTCCTCACTGCTTTGCCTGAG / GSVIVT00006900001
VvPGM / GATTCAGGCGCTGTTCAAT / CCATTTCCTGCTGCAATTT / GSVIVT01018452001
VvNTT / TTTCAGCAAGAGTGCCAAGT / TCCCTTTAACCTTGGTGTCC / GSVIVT01017211001
VvAGPB1 / ACTTCCCTCGGATTGAGAGTGC / ACCAATGTCTTCCCAGTAGCCATC / GSVIVT01030141001
VvAGPS1 / GAGGCAGGAATGAAATGGTT / TGCATCCTCAAATACCCAAA / GSVIVT01023805001
VvSS1 / TCGGATACTGACAGTTAGCAAGGG / ATGCAGACCATATCCACCTTCGG / GSVIVT01038557001
VvSS2 / TGCAAGGCAGCTATTGAGGTTCC / TCCCATCTCCATAGCAGACACCAC / GSVIVT01011429001
VvSS3 / CCTTAGAACGCAATGGACAGGTTG / AAGCAAAGTCGAGCACGGTCA / GSVIVT01012723001
VvSS4 / TGGCACCAGTTGCTAAGGTTG / TGCAGTGCTCTGCTAAGACCAG / GSVIVT010214040014
VvSS5 / ACAAGGTACCCTTGATCGTTTGC / CCAGAACAATTATCTGCCCACCAC / GSVIVT01032233001
VvGBSS1 / GTGATGTTCTCGGTGGTCTG / CCCAACTGGAATCTCAACAA / GSVIVT01019680001
VVSuSy
(Hren et al. 2009) / TGTTAAGGCTCCTGGATTTCAATTA / AGCCAAATCTTGGCAAGCA / GSVIVT01015018001
VvSWEET1 / TTCTTGGCTCCACTGTATGG / GCCCTCTCCTTCTTGATTGA / GSVIVT01010015001
VvSWEET4
(Chong et al 2014) / GGCTCGGACTGTGATTGGTA / ACATGCAGTTCATCACTGTGG / GSVIVT01032489001
VvSPS1 / GGTTTGCCTATTGTTGCCACCAG / CAAGAAGGCCATTGTCAAGTACCC / GSVIVT01012825001
VvSUC11
(Pastenes et al., 2014) / TGTGCCAATCTCAAGTCTGCC / CCTGGGCTGCTGTTATGCTT / GSVIVT01009254001
VvSUC12
(Pastenes et al. 2014) / ACCAGCCTCACCATTTATCAGAC / ATTTCATAACTGCTCTCAGGGTTG / GSVIVT01020031001
VvSUC27
(Pastenes et al. 2014) / TGCTTGGCACTGACGGTACT / GCTGTAGGTGATCGCAAGAGG / GSVIVT01034886001
VvSTS1 / CGAAGCAACTAGGCATGTGT / CTCCCCAATCCAATCCTTCA / GSVIVT01010590001
VvGAPDH
(Gainza-Cortés et al. 2012) / TTCCGTGTTCCTACTGTTG / CCTCTGACTCCTCCTTGAT / GSVIVT00009717001
Supplemental Figure S1. Scheme of the starch biosynthesis pathway in grapevine woody tissues.G6-Pi is transported into the amyloplast by the action of a GPT and converted into G1-Pi by PGM. Following this reaction, G1-Pi and ATP are combined into ADP-gluc, by the action of AGPase, and its glucosyl moiety is added to the starch granule by the action of starch synthases. ADP-gluc, ADP-Glucose; AGPase, ADP-glucopyrophosphorylase; G1-Pi, glucose-1-phosphate; G6-Pi, glucose-6-phosphate; GPT, glucose-6-phosphate/phosphate translocator; NTT, plastidic nucleotide transporter; PGM, phosphoglucomutase; Pi, inorganic phosphate; PPi, inorganic pyrophosphate.
Supplemental Figure S2. Phylogenetic tree of AGPase complex subunits.Thetree was constructed after aligning sequences from grapevine (Vitis vinifera), potato (Solanum tuberosum) and Arabidopsis thaliana.
Supplemental Figure S3. Phylogenetic tree of starch synthases. Thetree was constructed after aligning sequences from grapevine (Vitis vinifera), potato (Solanum tuberosum) and Arabidopsis thaliana.