Supplementary Table 1. Standard Chlamydomonas reinhardtii strains examined in this study

Subline I Sager line / History
CCa-1690 * ** / Sager strain 21 gr, received from Sager by CC in 1983
UTEX 2247 ** / sent to UTEX in 1980 by Sager as "ammonium-requiring, basic strain mating type minus"; probably originally from Duke, may be same as CC-124
UTEX 2246 * ** *** / sent to UTEX in 1980 by Sager as "basic strain mating type plus"
CC-377 * *** / cleocin resistant, sent to Duke by Sager in 1976
CC-379 * *** / streptomycin and spectinomycin resistant, , sent to Duke by Sager in 1976
CC-215 * / streptomycin resistant, sent to Duke by Sager in 1976
CC-216 * / streptomycin resistant, sent to Duke by Sager in 1976
CC-270 * / streptomycin resistant, sent to Duke by Sager in 1976
CC-1691 * / Sager strain 6145, received from Sager by CC in 1983
CC-1692 * ** *** / Sager strain 5177D, received from Sager by CC in 1983
Subline II Cambridge line
UTEX 89 * ** *** / received by UTEX in 1953 from CCAP
UTEX 90 * ** *** / received by UTEX in 1953 from CCAP
SAG 11-32a * / received by SAG from Indiana (now UTEX) prior to 1964
SAG 11-32b * / received by SAG from Indiana (now UTEX) prior to 1964
CC-1080 * / Smith strain sent to Provasoli, given to Starr but not accessioned as part of the Indiana (UTEX) collection; sent by Starr to CC in 1980
CC -1081 * *** / "
CC -1082 * / "
CC -1083 * *** / "
Subline III E/L line
CC-29 * / multiply marked mapping strain from Ebersold, sent to Duke in 1973; parent in crosses done by Ranumet al.
CC-124 * ** *** / brought to Duke by Gillham in 1968, from Levine's lab at Harvard
CC-124 J * ** *** / sent to Matsuda by CC in 1983 as CC-124
CC-125 * ** *** / brought to Duke by Gillham in 1968, from Levine's lab at Harvard
UTEX 2243 * ** / = CC-124 sent by CC to UTEX in 1980
UTEX 2244 * ** / = CC-125 sent by CC to UTEX in 1980
CC-620 * ** / subclone of Ebersold/Levine wild-type selected by Goodenough for high mating efficiency, sent to CC in 1978
CC-621 * ** *** / subclone of Ebersold/Levine wild-type selected by Goodenough for high mating efficiency, sent to CC in 1978
CCAP 11-32D * / sent by Levine to CCAP in 1972
CF-1 * / brought from Togasaki lab by Forest
CF-2 * / brought from Togasaki lab by Forest
CW-derived strains
SAG 83.81 * / cw15 mutant, from Davies to CCAP 1976; CCAP to SAG 1981
CC-277 ** / cw15 mutant, from Davies to Duke in 1972
CC-278 ** / cw15 mutant, from Davies to Duke in 1972
UTEX 2337 ** *** / = SAG 83.81, from SAG to UTEX 1983
CC-406 *** / cw15 mutant, from Davies to Surzycki to Chiang prior to 1976; from Chiang's lab to Duke 1977
CC-400 * / cw15 mutant, from Davies to Levine to Chiang prior to 1976; from Chiang's lab to Duke 1977
CC-3491 * / cw15 derived from CC-406 by four successive backcrosses to CC-125
Returned standard strains
SAG 11-31 C. sp. * / in SAG as Pringsheim isolate, C. sp.
SAG 73.72 C. reinhardtii * / in SAG as "C-8 C. mutabilis
deposited by H. Tsubo; received from Inst. Appl. Microbiol. Japan, Tokyo"
SAG 11-32c C. reinhardtii * / in SAG as Lewin deposition 1957, "from Caroline Islands, South Pacific, soil from Jaluit"
SAG 18.79 C. reinhardtii * / in SAG as C. "red tide Florida" deposited by Provasoli, 1977
SAG 77.81 C. reinhardtii * / in SAG as "G. Paris No. 170, France"

The ITS of the following clonal cultures were examined by direct PCR (* ), subcloning of PCR products ( **), and/or Southern blot (*** ).

aCC = Chlamydomonas Collection, Duke University; CCAP = Culture Collection of Algae and Protozoa, Scotland; CF = Charlene Forest, Brooklyn College of CUNY; SAG = Sammlung von Algenkulturen, Göttingen; UTEX = The Culture Collection of Algae at the University of Texas, Austin

Supplementary Table 2. Organisms used for phylogenetic analyses.

Taxon / Identitya / Source / GenBank #
C. smithii + / CC-1373 / MA, USA / AF033291
C. reinhardtii / CC-2935 / Quebec, Canada / AF033288
C. reinhardtii / CC-2342 / PA, USA / AF033290
C. reinhardtii / CC-1952 / MN, USA / AF033289
C. reinhardtii / CC-2931 / NC, USA / AF033287
C. reinhardtii std "long" / CC-620 / MA, USA / U66954
C. reinhardtii std "short" / CC-620 / MA, USA / AJ749638
C. sp. / CCAP 11/132 / Kenya / AJ749626
C. incerta / SAG 7.73 / Cuba / U66950
Volvox carteri / UTEX 1885 / Japan / U67017
Gonium pectorale / Alaska-1 / AK, USAb / AF054429
Gonium pectorale / Africa-2 / S. Africa b / AF054431
Gonium sacculiferum / UTEX 935 / CA, USA / U66973
C. sp. (Rt. 621A) / c / TX, USA / AF033277
C. debaryana=komma / UTEX 579 / Japan / U66951
C. debaryana / CCAP 11/130 / Germany / AJ749627
C. sphaeroides (iyengar) / UTEX 221 / India / U66952
C. typica / SAG 61.72 / TX, USA / AJ749622
C. parallestriata / SAG 14.88 / Portugal / AJ749623
C. sp. / UTEX 796 / IN, USA / AJ749621
C. parallestriata / SAG 2.73 / unknown / AJ749613
Vitreochlamys ordinata / NIES-882d / Germany / AJ749611
C. sp. (Chile J) / c / Chile / AF033295
C. petasus / SAG 11-45 / CT, USA / AJ749615
C. petasus / SAG 10.73 / Czech / AJ749614
C. zebra (texensis) / UTEX1904 / TX, USA / AF033294
C. callosa / UTEX 624 / Netherlands / U66945
C. cribrum / UTEX 1341 / Czech / AF033281
C. marvanii / SAG 73.81 / Germany / AJ749620
C. pygmaea / SAG 5.93 / Prudhoe Bay,Antarctica / AJ749619
C. ulvaensis / SAG 62.72 / Scotland / AJ749612
C. minuta / SAG 19.90 / NV,USA / AJ749617
C. segnis (asymmetrica) / SAG 18.90 / unknown / AJ749616
C. asymmetrica(peterfii) / SAG 70.72 / Czech / U66944
C. gyrus / SAG 17.90 / Germany / AJ749618
Paulschulzia pseudovolvox / UTEX 167 / Finland / AF182428

aCC, CCAP, SAG, UTEX : see supplementary Table 1.

b see Fabryet al. 1999

c see Coleman and Mai 1997

d Microbial Culture Collection, National Inst. for Environ. Studies, Japan