ClusterNototalESTsGene NameCG No.E-value Score

Chitin-binding structural protein, non-RR type (5 genes)

4518Chitin binding Peritrophin-ACG102874732.00E-133

2025Chitin binding Peritrophin-ACG47782838.00E-77

3194Chitin binding Peritrophin-ACG81921352.00E-32

4392Chitin binding Peritrophin-ACG170583306.00E-91

12481Chitin-binding, domain 3CG157861538.00E-38

Chitin metabolism (12 genes)

9210Glycoside hydrolaseCG322094199.00E-118

2035Glycoside hydrolaseCG79973252.00E-89

2085Chitinase---

2713Glycoside hydrolaseCG97012652.00E-71

2833ChitinaseCG17802652.00E-71

3362Glycoside hydrolaseCG57311722.00E-43

4102Chitin bindingCG1364358.93.00E-09

5341Glycoside hydrolaseCG97012269.00E-60

5731Glycoside hydrolaseCG93073713.00E-103

11461ChitinaseCG181401914.00E-49

13281galactosyltransferaseCG117802499.00E-67

13721Glycoside hydrolaseCG1814055.14.00E-08

Cuticle Protein Structure Modifier (5 genes)

3512Peptidyl-prolyl cis-trans isomeraseCG28522482E-66

5491Laccase2CG304373434.00E-95

6771Prolyl 4-hydroxylaseCG310221492.00E-36

7241Disulfide IsomeraseCG89832466.00E-66

12791Peptidylprolyl isomeraseCG98473044E-83

Excreted Protease (16 genes)

979prophenoloxidase activating enzymeCG30661077.00E-24

1457trypsin-like serine peptidaseCG53902771.00E-74

1905trypsin-like serine peptidaseCG539083.61.00E-16

1726serine carboxypeptidase---

3292cathepsinDCG15482944.00E-80

3312serine carboxypeptidaseCG45721305.00E-31

3442metallopeptidasesCG67631475.00E-36

4522cathepsinBCG109922583.00E-69

5171trypsin-like serine peptidase---

7391serine carboxypeptidaseCG45721744.00E-44

8691trypsin-like serine peptidaseCG30661414.00E-34

10131trypsin-like serine peptidase---

10411cysteine peptidaseCG3281689.72.00E-18

11111Zinc carboxypeptidaseCG31081675.00E-42

12301Peptidase S28CG99532214.00E-58

12981trypsin-like serine peptidaseCG499892.82.00E-19

Hormone and lipid metabolism (19 genes)

8910Cytochrome P450CG117153131.00E-85

2913Cytochrome P450CG513799.82.00E-21

3642steroid dehydrogenaseCG132842052.00E-53

3682sterol carrier proteinCG173202284.00E-60

4452Di-trans-poly-cis-decaprenylcistransferase---

3183farnesoic acid O-methyltransferaseCG105271761.00E-44

5311farnesoic acid O-methyltransferaseCG1052758.53.00.E-09

54413-oxoacid CoA-transferaseCG11402113.00E-55

7991Cytochrome P450CG85872852.00E-77

8721farnesyl pyrophosphate synthaseCG123891605.00E-40

9431steroid dehydrogenaseCG71131602.00E-40

10381estradiol 17-beta-dehydrogenaseCG1115187.44.00E-18

11391acyltransferase, lipid metabolismCG52781944.00E-50

11791esteraseCG43901742.00E-44

12031haloalkane dehalogenase---

12331Carboxylesterase, type BCG47572299.00E-61

12701Terpenoid synthaseCG105851782.00E-45

13121steroid dehydrogenaseCG71131897.00E-49

13591fatty acid elongaseCG27812782.00E-75

Protease Inhibitor (20 genes)

4418Protease Inhibitor, Kazal-typeCG122066.22.00E-11

1109serpinCG94531701.00E-42

1666Cathepsin propeptide inhibitor---

2204Protease Inhibitor, Kazal-typeCG836952.42.00E-07

3572serpinCG109131031.00E-22

3662Protease Inhibitor, Kazal-type---

3912serpinCG66801052.00E-23

4532serpinCG134271.25.00E-13

4582serpinCG93341352.00E-32

4642serpinCG946062.43.00E-10

4752Protease Inhibitor, Kunitz typeCG1254083.61.00E-16

5021Cathepsin propeptide inhibitorCG12163477.00E-06

5121serpin---

6111Protease Inhibitor, Kunitz typeCG331031039.00E-23

6281Protease Inhibitor, Kunitz type---

8481Protease Inhibitor, Kazal-typeCG323541915.00E-49

8891Protease Inhibitor, Kunitz typeCG1671262.45.00E-11

11221Protease Inhibitor, Kunitz typeCG281671.62.00E-13

11621Protease Inhibitor, Kazal-type---

11071Cathepsin propeptide inhibitor---

Small ligand binding (41 genes)

1178JH-bindingCG144572015.00E-52

1218JH-binding(An0921)CG1185268.25.00E-12

2055JH-bindingCG265073.61.00E-13

2154JH-binding(Brp2095)CG104071023.00E-22

2683JH-binding---

3372JH-bindingCG1040795.14.00E-20

4482JH-bindingCG1040790.97.00E-19

4792JH-bindingCG1445751.64.00E-07

5351JH-bindingCG1040795.92.00E-20

6461JH-bindingCG1185463.21.00E-10

6611JH-binding(wdS30639)CG10407992.00E-21

6651JH-binding---

7371JH-bindingCG1185260.86.00E-10

8051JH-binding(JHBP)---

9511JH-bindingCG265088.63.00E-18

9671JH-bindingCG1040762.43.00E-10

4020Odorant-bindingCG113901022.00E-22

5614Odorant-bindingCG113901222.00E-28

8510Odorant-bindingCG1139085.91.00E-17

1566Odorant-bindingCG1139096.71.00E-20

1965Odorant-bindingCG846273.68.00E-14

1985Odorant-bindingCG113901146.00E-26

2503Odorant-bindingCG3017270.97.00E-13

3762Odorant-bindingCG113901113.00E-25

4512Odorant-binding---

4622Odorant-bindingCG935864.34.00E-11

7541Odorant-bindingCG1139085.92.00E-17

7621Odorant-bindingCG113901061.00E-23

9711Odorant-binding---

10631Odorant-bindingCG935879.31.00E-15

8331VitaminE-bindingCG1292677.46.00E-15

2783Cellular retinaldehyde-bindingCG26632352.00E-62

4302Cellular retinaldehyde-bindingCG26631022.00E-22

5921Cellular retinaldehyde-bindingCG2663823.00E-16

7571Cellular retinaldehyde-bindingCG38231621.00E-40

11611Cellular retinaldehyde-bindingCG38231431.00E-34

12841Cellular retinaldehyde-bindingCG102372442.00E-65

13231Cellular retinaldehyde-bindingCG100261939.00E-50

3802FK506-bindingCG110011814.00E-46

12181FK506-bindingCG18471422.00E-34

Transcription Factor Activity Genes (17 genes)

2893modifier of mdg4CG324912E-46182

3672LimpetCG321711E-111399

4162LimpetCG321711E-25113

4912bunchedCG54618E-24107

5131CG7839CG78392E-1579.3

6121Suppressor of HairlessCG34974E-66247

6391E(spl) region transcript mbetaCG145482E-30128

7331CG4914CG49142E-28120

7661anterior openCG31665E-47184

8011nautilusCG102503E-2198.6

9001drumstickCG100161E-36149

10611MTA1-likeCG22442E-37151

10821CG5343CG53435E-105377

11531slow border cellsCG43544E-30128

12381apterousCG83763E-45178

12501DP transcription factorCG46542E-1269.7

13541no ocelliCG44911E-36150

pigmentation genes (9 genes)

Melanin biosynthesis

7012yellowCG37573E-125445

2663tanCG121201E-57219

2753pale (tyrosine hydroxylase)CG101182E-52202

5591yellow-fCG185509E-1887

6541yellow-f2CG80634E-1681.6

7861Henna (phenylalanine hydroxylase)CG73991E-79292

Ommochrome biosynthesis

10121cinnabarCG15559E-28119

10271rubyCG114271E-30129

Uric acid biosynthesis

12071rosy (xanthine dehydrogenase I)CG76422E-75278

Additional File 3 Characteristic gene groups identified in epM dataset

* E-value and score are calculated in Drosophila homologs.