Krenn et al. Integrin-linked kinase in CLL

Supplemental Table 1: Patient characteristics. M:Male; F:Female; UMCLL: unmutated IgVH; MCLL: mutated IgVH; n.A.: data not available

PatientID / Informed Consent / Sex / IgVH Status / Zap70 / CD38 / VLA4 / del11q / del13q / del17p / tri12
1 / yes / F / MCLL / low / low / low / no / yes / no / no
2 / yes / M / MCLL / low / low / low / no / yes / yes / no
3 / yes / M / MCLL / low / low / high / no / yes / no / no
4 / yes / F / MCLL / borderline / low / high / no / no / no / no
5 / yes / M / UMCLL / high / high / high / no / no / no / no
6 / yes / M / MCLL / low / low / low / no / yes / yes / no
7 / yes / F / UMCLL / n.A. / high / n.A. / no / yes / no / yes
8 / yes / M / MCLL / low / low / low / no / yes / no / no
9 / yes / F / MCLL / low / low / low / no / no / no / yes
10 / yes / M / MCLL / low / low / low / no / yes / no / no
11 / yes / M / MCLL / low / low / low / no / yes / no / no
12 / yes / M / MCLL / low / high / high / no / no / no / yes
13 / yes / M / UMCLL / n.A. / high / n.A. / no / no / no / yes
14 / yes / F / MCLL / low / high / high / no / no / no / yes
15 / yes / F / MCLL / low / low / low / no / yes / no / no
16 / yes / F / MCLL / low / low / high / no / no / no / yes
17 / yes / M / MCLL / low / low / low / no / yes / no / no
18 / yes / F / UMCLL / high / high / high / no / no / no / yes
19 / yes / M / UMCLL / high / high / high / no / no / no / yes
20 / yes / M / MCLL / low / low / low / no / yes / no / no
21 / yes / M / UMCLL / high / high / high / no / no / no / no
22 / yes / M / MCLL / low / low / low / no / yes / no / no
23 / yes / F / MCLL / low / low / low / no / yes / no / no
24 / yes / F / MCLL / low / low / low / no / yes / no / no
25 / yes / M / UMCLL / high / low / high / no / no / no / yes
26 / yes / F / MCLL / low / low / high / no / no / no / no
27 / yes / M / MCLL / low / high / high / no / yes / no / no
28 / yes / M / MCLL / high / high / high / no / no / no / no
29 / yes / F / MCLL / low / low / low / no / yes / no / no
30 / yes / M / MCLL / low / low / low / no / yes / no / no
31 / yes / M / MCLL / high / low / low / no / no / no / no
32 / yes / F / UMCLL / high / high / high / no / yes / no / no
33 / yes / F / MCLL / low / low / low / no / yes / yes / no
34 / yes / F / MCLL / low / low / low / no / yes / no / no
35 / yes / M / MCLL / low / low / low / no / yes / no / no
36 / yes / M / MCLL / high / high / high / no / yes / no / no
37 / yes / M / MCLL / borderline / low / low / no / no / no / no
38 / yes / M / MCLL / low / low / high / no / no / no / no
39 / yes / M / MCLL / high / low / low / yes / no / no / no
40 / yes / M / MCLL / low / low / low / no / no / no / no
41 / yes / F / UMCLL / borderline / high / low / no / no / no / no
42 / yes / F / MCLL / low / low / low / no / yes / no / no
43 / yes / F / MCLL / low / low / low / no / yes / no / no
44 / yes / M / UMCLL / high / high / high / no / yes / yes / yes
45 / yes / F / MCLL / low / low / low / no / no / no / no
46 / yes / M / UMCLL / low / high / low / yes / yes / no / no
47 / yes / M / MCLL / low / low / low / no / yes / no / no
48 / yes / M / UMCLL / high / high / high / no / no / no / no
49 / yes / M / MCLL / low / low / high / yes / no / yes / no
50 / yes / M / MCLL / low / low / low / no / yes / no / no
51 / yes / F / MCLL / low / low / low / no / no / no / no
52 / yes / M / UMCLL / high / high / high / no / no / no / no
53 / yes / F / MCLL / low / low / low / no / yes / yes / no
54 / yes / F / UMCLL / borderline / low / high / no / yes / no / no
55 / yes / F / MCLL / low / low / low / no / yes / no / no
56 / yes / M / MCLL / borderline / low / low / no / yes / no / no
57 / yes / M / MCLL / low / low / low / no / yes / no / no
58 / yes / M / UMCLL / low / high / high / no / no / no / yes
59 / yes / F / MCLL / low / low / low / no / yes / no / no
60 / yes / F / MCLL / high / low / low / no / yes / no / no
61 / yes / M / MCLL / low / low / low / no / yes / no / no
62 / yes / F / UMCLL / low / high / low / yes / yes / no / no
63 / yes / M / UMCLL / high / high / low / yes / yes / no / no
64 / yes / M / UMCLL / high / high / low / yes / no / no / no
65 / yes / M / MCLL / low / low / low / no / yes / no / no
66 / yes / F / UMCLL / low / low / high / no / no / no / yes
67 / yes / M / n.A. / high / high / high / no / yes / no / no
68 / yes / M / MCLL / low / low / low / no / yes / no / no
69 / yes / M / UMCLL / high / high / high / no / yes / yes / no
70 / yes / M / MCLL / low / low / low / no / no / no / no
71 / yes / F / UMCLL / high / high / n.A. / no / no / no / no
72 / yes / M / MCLL / high / low / low / yes / yes / no / no
73 / yes / M / MCLL / high / low / high / no / yes / no / no
74 / yes / F / UMCLL / high / high / low / yes / yes / no / no
75 / yes / F / MCLL / high / high / high / no / no / no / yes
76 / yes / F / MCLL / high / low / n.A. / no / no / no / yes
77 / yes / M / UMCLL / high / high / low / yes / yes / no / no
78 / yes / F / UMCLL / low / low / low / no / yes / no / no
79 / yes / F / UMCLL / low / low / low / no / yes / no / no
80 / yes / F / MCLL / low / low / low / no / yes / no / no
81 / yes / M / UMCLL / high / high / high / no / yes / no / no
82 / yes / M / n.A. / high / low / low / no / no / no / no
83 / yes / F / UMCLL / low / low / low / no / no / no / no
84 / yes / M / UMCLL / high / high / low / no / yes / no / no
85 / yes / M / UMCLL / borderline / low / low / yes / no / no / no
86 / yes / M / MCLL / low / low / low / no / yes / no / no
87 / yes / F / MCLL / low / low / low / no / yes / no / no
88 / yes / F / MCLL / low / low / low / no / yes / no / no
89 / yes / F / MCLL / high / low / low / no / yes / no / yes
90 / yes / M / UMCLL / high / high / low / no / no / no / no
91 / yes / M / MCLL / low / high / low / no / yes / no / no
92 / yes / M / n.A. / high / high / low / yes / yes / no / no
93 / yes / F / n.A. / high / high / low / no / no / yes / no
94 / yes / M / MCLL / high / low / high / no / yes / no / no
95 / yes / M / MCLL / high / low / high / no / yes / no / no
96 / yes / M / n.A. / high / low / low / no / yes / no / no
97 / yes / M / n.A. / high / high / n.A. / no / yes / no / no
98 / yes / M / UMCLL / high / high / high / yes / yes / no / no
99 / yes / M / UMCLL / high / high / high / no / no / no / yes
100 / yes / F / n.A. / high / high / high / no / yes / no / no
101 / yes / M / n.A. / high / high / high / no / no / no / no
102 / yes / M / UMCLL / high / high / low / no / no / no / no
103 / yes / M / UMCLL / high / high / low / no / no / no / no
104 / yes / M / MCLL / n.A. / n.A. / n.A. / no / yes / no / no
105 / yes / M / MCLL / borderline / low / low / no / yes / no / no
106 / yes / F / MCLL / n.A. / n.A. / low / no / n.A. / no / n.A.
107 / yes / M / MCLL / n.A. / low / low / no / no / no / no
108 / yes / M / MCLL / low / low / low / no / yes / no / no

Supplemental Table 2: List of used antibodies. IF: Immunofluorescence; FACS: flow cytometry; WB: Western blotting; IHC: Immunohistochemistry; ChIP: Chromatin Immunopreicipitation; Blocking: Blocking experiment

Target / Company / Cat# / Usage
7-AAD / eBioscience / 00-6993-50 / FACS
aCD40L / eBioscience / BMS153 / Blocking
aIFNy / Beckman Coulter / 731749 / Blocking
aIL8 / R&D / MAB208 / Blocking
aMouse-AF488 / Jackson ImmunoResearch / 715-545-151 / IF
aMouse-HRP / Cell signaling / 7076 / WB
Annexin V-FITC / eBioscience / BMS306FI / FACS
aRabbit-AF488 / Invitrogen / A11008 / FACS, IF
aRabbit-AF647 / Jackson ImmunoResearch / 711-605-152 / IF
aRabbit-HRP / Cell signaling / 7074 / WB
aTNFa (Adalimumab) / AbbVie / xxx / Blocking
CD19 / AbD Serotec / MCA2454T / IHC
CD19-APC / Beckman Coulter / IM2470 / FACS
CD19-APC / Biolegend / 115512 / FACS
CD19-PC7 / Beckman Coulter / IM3628 / FACS
CD29-FITC / eBioscience / 11-0299 / FACS
CD38-PE / Beckman Coulter / IM1832U / FACS
CD49d-PE / BD / 555503 / FACS
CD5-AA700 / Beckman Coulter / A78835 / FACS
CD5-FITC / Beckman Coulter / A08932 / FACS
CD5-PE / BD / 553023 / FACS
CXCR4-PE / BD / 555974 / FACS
gTubulin / Sigma-Aldrich / T6557 / IF
IgG rabbit / Santa Cruz / sc-2027X / ChIP
IkBa / Cell signaling / 4812 / WB
ILK / Cell signaling / 3856 / WB, IF, FACS, IHC
ILK / BD / 611803 / WB
Ki67 / DAKO / MWB1 / IHC
p65 / Santa Cruz / sc-372X / ChIP
pan-Actin / Cell Signaling / 8456 / WB
pericentrin / abcam / ab4448 / IF
p-IkBa / Cell signaling / 2859 / WB
p-p65-PC7 / BD / 560335 / FACS
PPIA/Cyclophilin A / Cell Signaling / 2175 / WB
viability dye-eF450 / eBioscience / 65-0863 / FACS
ZAP70-FITC / Upstate / 16-210 / FACS

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