Supplemental Table 2. MMP proteolysis of Golli-MBP BG21 and a MALDI-TOF MS analysis of the digest fragments. The arrows indicate the positions of the scissile bonds.The numbering starts from the N-terminal methionine.

1)

MMP-2 fragments
MGNHSGKREL10 SAEKASKDGE20IHRGEAGKKR30 SVGK↓LSQTAS40 EDSDVFGEAD50
AIQNNGTSAE60 DTAVTDSKHT70ADPKNNWQGA80 H↓PADPGNRPH90 LIRLFSRDAP100
GREDNTFKDR110PSESDELQTI120QEDPTAASGG130 LDVMAS↓QKRP140SQRSKY↓LATA150
STMDHARHGF160LPRHRDTGIL170 DSIGR↓FFSGD180 RGAPKRGSGK190 VSLEHHHHHH200
Peptide sequences / Molecular mass, Da
Calculated / Measured
1 / 1-175 / 18813 / 18811
2 / 35-175 / 15328 / 15328
3 / 1-136 / 14372 / 14372
4 / 35-146 / 12146 / 12157
5 / 35-136 / 10887 / 10890
6 / 35-81 / 4929 / 4925

2)

MMP-8 fragments
MGNHSGKREL10 SAEKASKDGE20 IHRGEAGKKR30 SVGK↓LSQTAS40 EDSDVFGEAD50
AIQNNGTSAE60 DTAVTDSKHT70 ADPKN↓NWQGA80HPADPGNRPH90 LIRLFSRDAP100
GREDNTFKDR110 PSESDELQTI120QEDPTAASGG130 LDVMASQKRP140 SQRSKY↓LATA150
STMDHARHGF160 LPRHRDTGIL170 DSIGRFFSGD180 RGAPKRGSGK190 VSLEHHHHHH200
Peptide sequences / Molecular mass, Da
Calculated / Measured
1 / 35-200 / 18127 / 18118
2 / 76-200 / 13909 / 13904
3 / 35-146 / 12146 / 12157

3)

MMP-9 fragments
MGNHSGKREL10 SAEKASKDGE20 IHRGEAGKKR30 SVGK↓LSQTAS40 EDSDVFGEAD50 AIQNNGTSAE60 DTAVTDSKHT70 ADPKN↓NWQGA80HPADPGN↓RPH90LIRLFSRDAP100GREDNTFKDR110 PSESDELQT↓I120QEDPTAASGG130 LDVMASQKRP140 SQRSKY↓LATA150 STMDHARHGF160 LPRH↓RDTGIL170DSIGRFFSGD180 RGAPKRGSGK190 VSLEHHHHHH200
Peptide sequences / Molecular mass, Da
Calculated / Measured
1 / 76-200 / 13909 / 13910
2 / 35-146 / 12146 / 12165
3 / 35-119 / 9244 / 9244
4 / 88-164 / 8682 / 8684
5 / 1-75 / 7721 / 7726

4)

MMP-10 fragments
MGNHSGKREL10 SAEKASKDGE20 IHRGEAGKKR30 SVGK↓LSQTAS40EDSDVFGEAD50 AIQNNGTSAE60 DTAVTDSKHT70 ADPKNNWQGA80 HPADPGNRPH90 LIRLFSRDAP100GREDNTFKDR110 PSESDELQTI120 QEDPTAASGG130LDVMASQKRP140 SQ↓RSKY↓LATA150 STMDHARHGF160 LPRH↓RDTGIL170DSIGRFFSGD180 RGAPKRGSGK190 VSLEHHHHHH200
Peptide sequences / Molecular mass, Da
Calculated / Measured
1 / 35-146 / 12146 / 12161
2 / 143-200 / 6533 / 6538

5)

MMP-12 fragments
MGNHSGKREL10 SAEKASKDGE20 IHRGEAGKKR30 SVGK↓LSQTAS40EDSDVFGEAD50 AIQNNGTSAE60 DTAVTDSKHT70 ADPKN↓NWQGA80H↓PADPGNRPH90LIRLFSRDAP100GREDNTFKDR110PSESDELQT↓I120QEDPTAASGG130 LDVMASQKRP140 SQRSKY↓LATA150 STMDHARHGF160LPRHRDTGIL170 DSIGRFFSGD180 RGAPKRGSGK190 VSLEHHHHHH200
Peptide sequences / Molecular mass, Da
Calculated / Measured
1 / 35-200 / 18127 / 18130
2 / 76-200 / 13909 / 13906
3 / 35-146 / 12146 / 12167
4 / 35-119 / 9244 / 9247
5 / 35-81 / 4929 / 4924

6)

MT1-MMP fragments
MGNHSGKREL10 SAEKASKDGE20 IHRGEAGKKR30 SVGK↓LSQTAS40 EDSDVFGEAD50
AIQNNGTSAE60 DTAVTDSKHT70 ADPKNNWQGA80 H↓PADPGNRPH90 LIRLFSRDAP100
GREDNTFKDR110PSESDELQT↓I120QEDPTAASGG130LDVMA↓S↓QKRP140SQ↓RSKY↓LATA150
STMDHARHGF160LPRHRDTGIL170 DSIGRFFSGD180 RGAPKRGSGK190 VSLEHHHHHH200
Peptide sequences / Molecular mass, Da
Calculated / Measured
1 / 1-146 / 15630 / 15620
2 / 1-136 / 14372 / 14367
3 / 1-135 / 14285 / 14281
4 / 35-146 / 12146 / 12159
5 / 35-119 / 9244 / 9241
6 / 136-200 / 7345 / 7345
7 / 137-200 / 7258 / 7259
8 / 143-200 / 6533 / 6536
9 / 147-200 / 5999 / 6001
10 / 35-81 / 4929 / 4922

7)

MT2-MMP fragments
MGNHSGKREL10 SAEKASKDGE20 IHRGEAGKKR30 SVGK↓LSQTAS40 EDSDVFGEAD50
AIQNNGTSAE60 DTAVTDSKHT70 ADPKNNWQGA80 H↓PADPGNRPH90 LIRLFSRDAP100
GREDNTFKDR110PSESDE↓LQT↓I120QEDPTAASGG130 LD↓VMA↓S↓QKRP140SQRSKY↓LATA150
STMDHARHGF160LPRHRDTGIL170 DSIGRFFSGD180 RGAPKRGSGK190 VSLEHHHHHH200
Peptide sequences / Molecular mass, Da
Calculated / Measured
1 / 1-135 / 14285 / 14279
2 / 35-146 / 12146 / 12164
3 / 35-119 / 9244 / 9246
4 / 35-116 / 8902 / 8904
5 / 133-200 / 7646 / 7654
6 / 136-200 / 7345 / 7349
7 / 137-200 / 7258 / 7264
8 / 147-200 / 5999 / 6004
9 / 35-81 / 4929 / 4926

8)

MT3-MMP fragments
MGNHSGKREL10 SAEKASKDGE20 IHRGEAGKKR30 SVGK↓LSQTAS40 EDSDVFGEAD50
AIQNNGTSAE60 DTAVTDSKHT70 ADPKNNWQGA80 H↓PADPGNRPH90 LIRLFSRDAP100
GREDNTFKDR110 PSESDELQT↓I120QEDPTAASGG130LDVMA↓S↓QKRP140SQRSKY↓LATA150
STMDHARHGF160 LPRHRDTGIL170 DSIGRFFSGD180RGAPKRGSGK190 VSLEHHHHHH200
Peptide sequences / Molecular mass, Da
Calculated / Measured
1 / 1-136 / 14372 / 14374
2 / 35-146 / 12146 / 12165
3 / 35-119 / 9244 / 9250
4 / 82-135 / 5889 / 5893
5 / 35-81 / 4929 / 4924

9)

MT6-MMP fragments
MGNHSGKREL10 AEKASKDGE20 IHRGEAGKKR30 SVGK↓LSQTAS40 EDSDVFGEAD50
AIQNNGTSAE60 DTAVTDSKHT70 ADPKNNWQGA80 H↓PADPGNRPH90 LIRLFSRDAP100
GREDNTFKDR110 PSESDE↓LQT↓I120QEDPTAASGG130 LDVMA↓S↓QKRP140SQRSKY↓LATA150
STMDHARHGF160 LPRHRDTGIL170 DSIGR↓FFSGD180RGAPKRGSGK190 VSLEHHHHHH200
Peptide sequences / Molecular mass, Da
Calculated / Measured
1 / 1-136 / 14372 / 14367
2 / 35-146 / 12146 / 12164
3 / 35-119 / 9244 / 9246
4 / 35-116 / 8902 / 8907
5 / 136-200 / 7345 / 7346
6 / 137-200 / 7258 / 7264
7 / 82-135 / 5889 / 5895
8 / 35-81 / 4929 / 4922
9 / 137-175 / 4459 / 4454