Supplementary Materials
Table S1. Summary data for the three WorldClim Bioclimatic variables used under present (1950-2000) climate conditions and the projected conditions for the 2040s and 2080s under the A2 emissions scenario, presented as means calculated from values taken at 9905 systematic sampling points.
Bioclimatic variable / 1950-2000 / 2040s / 2080sAnnual mean temperature (°C)
Mean / 17.2 / 18.6 / 21.1
Maximum / 24.4 / 25.9 / 28.6
Minimum / 5.4 / 6.7 / 9.3
Mean temperature of the warmest quarter, Tmax (°C)
Mean / 22.3 / 23.8 / 26.2
Maximum / 29.4 / 30.9 / 33.0
Minimum / 9.5 / 10.7 / 13.0
Mean temperature of the coolest quarter, Tmin (°C)
Mean / 11.3 / 12.4 / 14.9
Maximum / 19.5 / 20.6 / 23.6
Minimum / 0.7 / 1.6 / 4.5
Table S2. Mean temperature of the warmest quarter (Tmax) and WTs under present (1950-2000) climate conditions and the projected conditions for the 2040s and 2080s under the A2 emissions scenario for all locations at which Cyrtobagous salviniae releases have been recorded.
Tmax / WT1 (CTmax-Tmax) / WT2 (ULT50-Tmax) / Tmax / WT1 (CTmax-Tmax) / WT2 (ULT50-Tmax) / Tmax / WT1 (CTmax-Tmax) / WT2 (ULT50-Tmax)
Nanaga / 20.9 / 21.5 / 22.8 / 22.2 / 20.2 / 21.5 / 24.1 / 18.4 / 19.7
Port St Johns / 22.2 / 20.2 / 21.5 / 23.3 / 19.1 / 20.4 / 25.3 / 17.1 / 18.4
Swartkops River / 22.0 / 20.4 / 21.7 / 23.2 / 19.2 / 20.5 / 25.0 / 17.4 / 18.7
Uitenhage rural / 21.3 / 21.1 / 22.4 / 22.5 / 19.9 / 21.2 / 24.4 / 18.0 / 19.3
Glenhazel Park / 20.1 / 22.3 / 23.6 / 21.6 / 20.8 / 22.1 / 24.2 / 18.2 / 19.5
Cato Ridge Golf Course / 21.3 / 21.1 / 22.4 / 22.3 / 20.1 / 21.4 / 24.3 / 18.1 / 19.4
Umdloti River / 24.2 / 18.2 / 19.5 / 25.2 / 17.3 / 18.6 / 27.2 / 15.3 / 16.6
Frikkie Geyser dam, Sterk River / 21.9 / 20.5 / 21.8 / 23.3 / 19.1 / 20.4 / 26.0 / 16.4 / 17.7
Groblersdal / 22.9 / 19.5 / 20.8 / 24.2 / 18.2 / 19.5 / 26.6 / 15.8 / 17.1
Kameeldoorn / 24.4 / 18.0 / 19.3 / 25.7 / 16.7 / 18.0 / 28.2 / 14.3 / 15.6
Kapama / 25.0 / 17.4 / 18.7 / 26.2 / 16.2 / 17.5 / 28.6 / 13.9 / 15.2
Klasarie Rivier / 24.9 / 17.5 / 18.8 / 26.1 / 16.3 / 17.6 / 28.5 / 13.9 / 15.2
Selati River / 25.6 / 16.8 / 18.1 / 26.9 / 15.5 / 16.8 / 29.3 / 13.1 / 14.4
Thaba Noka - Sterk River / 21.6 / 20.8 / 22.1 / 23.1 / 19.3 / 20.6 / 25.7 / 16.7 / 18.0
Yamorna Weir, Groot Letaba / 24.6 / 17.8 / 19.1 / 25.9 / 16.5 / 17.8 / 28.3 / 14.1 / 15.4
Klaserie river / 25.7 / 16.7 / 18.0 / 26.8 / 15.6 / 16.9 / 29.2 / 13.2 / 14.5
Mtshawu stream, Pretoriuskop / 24.8 / 17.6 / 18.9 / 25.9 / 16.5 / 17.8 / 28.2 / 14.2 / 15.5
Nelspruit / 22.8 / 19.6 / 20.9 / 23.9 / 18.5 / 19.8 / 26.2 / 16.2 / 17.5
Sabie River / 26.1 / 16.3 / 17.6 / 27.1 / 15.3 / 16.6 / 29.4 / 13.0 / 14.3
Skukuza / 25.8 / 16.6 / 17.9 / 26.9 / 15.5 / 16.8 / 29.2 / 13.2 / 14.5
White River / 22.0 / 20.4 / 21.7 / 23.2 / 19.2 / 20.5 / 25.5 / 16.9 / 18.2
Cape Town / 20.4 / 22.0 / 23.3 / 21.7 / 20.7 / 22.0 / 23.2 / 19.2 / 20.5
HM 23, N2 / 19.2 / 23.2 / 24.5 / 20.6 / 21.8 / 23.1 / 22.5 / 19.9 / 21.2
Johan Reichert / 19.5 / 22.9 / 24.2 / 20.9 / 21.5 / 22.8 / 22.8 / 19.6 / 20.9
Rheenendal Rd / 19.6 / 22.8 / 24.1 / 21.0 / 21.4 / 22.7 / 22.9 / 19.5 / 20.8
Somerset West / 20.7 / 21.7 / 23.0 / 22.0 / 20.4 / 21.7 / 23.5 / 18.9 / 20.2
Somerset West / 20.4 / 22.0 / 23.3 / 21.7 / 20.7 / 22.0 / 23.2 / 19.2 / 20.5
Stanwix / 19.6 / 22.8 / 24.1 / 21.0 / 21.4 / 22.7 / 23.0 / 19.4 / 20.7
The Crags, Redford Road / 19.4 / 23.0 / 24.3 / 20.8 / 21.6 / 22.9 / 22.7 / 19.7 / 21.0
Table S3. Mean temperature of the coldest quarter (Tmin) and LTTs under present (1950-2000) climate conditions and the projected conditions for the 2040s and 2080s under the A2 emissions scenario for all locations at which Cyrtobagous salviniae releases have been recorded.
Tmin / LTT1 (CTmin-Tmin) / LTT2 (LLT50-Tmin) / Tmin / LTT1 (CTmin-Tmin) / LTT2 (LLT50-Tmin) / Tmin / LTT1 (CTmin-Tmin) / LTT2 (LLT50-Tmin)
Nanaga / 13.7 / -1.2 / -20.9 / 14.6 / -2.1 / -21.8 / 16.5 / -4.1 / -23.7
Port St Johns / 17.3 / -4.8 / -24.5 / 18.2 / -5.7 / -25.4 / 20.5 / -8.1 / -27.7
Swartkops River / 14.3 / -1.8 / -21.5 / 15.3 / -2.8 / -22.5 / 17.0 / -4.6 / -24.2
Uitenhage rural / 12.6 / -0.1 / -19.8 / 13.6 / -1.1 / -20.8 / 15.4 / -3.0 / -22.6
Glenhazel Park / 11.2 / 1.3 / -18.4 / 12.3 / 0.2 / -19.5 / 15.5 / -3.1 / -22.7
Cato Ridge Golf Course / 14.2 / -1.7 / -21.4 / 15.0 / -2.5 / -22.2 / 17.5 / -5.1 / -24.7
Umdloti River / 17.5 / -5.0 / -24.7 / 18.4 / -5.9 / -25.6 / 20.9 / -8.5 / -28.1
Frikkie Geyser dam, Sterk River / 12.5 / 0.0 / -19.7 / 13.6 / -1.1 / -20.8 / 16.8 / -4.4 / -24.0
Groblersdal / 13.4 / -0.9 / -20.6 / 14.5 / -2.0 / -21.7 / 17.6 / -5.2 / -24.8
Kameeldoorn / 14.8 / -2.3 / -22.0 / 15.9 / -3.4 / -23.1 / 19.0 / -6.6 / -26.2
Kapama / 17.1 / -4.6 / -24.3 / 18.1 / -5.6 / -25.3 / 21.2 / -8.8 / -28.4
Klasarie Rivier / 17.1 / -4.6 / -24.3 / 18.1 / -5.6 / -25.3 / 21.2 / -8.8 / -28.4
Selati River / 17.5 / -5.0 / -24.7 / 18.5 / -6.0 / -25.7 / 21.6 / -9.2 / -28.8
Thaba Noka - Sterk River / 12.3 / 0.2 / -19.5 / 13.4 / -0.9 / -20.6 / 16.6 / -4.2 / -23.8
Yamorna Weir, Groot Letaba / 15.8 / -3.3 / -23.0 / 16.9 / -4.4 / -24.1 / 20.1 / -7.7 / -27.3
Klaserie river / 17.3 / -4.8 / -24.5 / 18.3 / -5.8 / -25.5 / 21.4 / -9.0 / -28.6
Mtshawu stream, Pretoriuskop / 17.0 / -4.5 / -24.2 / 17.9 / -5.4 / -25.1 / 20.9 / -8.5 / -28.1
Nelspruit / 15.5 / -3.0 / -22.7 / 16.4 / -3.9 / -23.6 / 19.4 / -7.0 / -26.6
Sabie River / 17.4 / -4.9 / -24.6 / 18.4 / -5.9 / -25.6 / 21.3 / -8.9 / -28.5
Skukuza / 16.4 / -3.9 / -23.6 / 17.4 / -4.9 / -24.6 / 20.4 / -8.0 / -27.6
White River / 14.7 / -2.2 / -21.9 / 15.7 / -3.2 / -22.9 / 18.7 / -6.3 / -25.9
Cape Town / 11.9 / 0.6 / -19.1 / 13.1 / -0.6 / -20.3 / 14.3 / -1.9 / -21.5
HM 23, N2 / 13.0 / -0.5 / -20.2 / 14.1 / -1.6 / -21.3 / 15.6 / -3.2 / -22.8
Johan Reichert / 13.0 / -0.5 / -20.2 / 14.0 / -1.5 / -21.2 / 15.6 / -3.2 / -22.8
Rheenendal Rd / 13.2 / -0.7 / -20.4 / 14.2 / -1.7 / -21.4 / 15.8 / -3.4 / -23.0
Somerset West / 12.2 / 0.3 / -19.4 / 13.3 / -0.8 / -20.5 / 14.5 / -2.1 / -21.7
Somerset West / 11.9 / 0.6 / -19.1 / 13.1 / -0.6 / -20.3 / 14.3 / -1.9 / -21.5
Stanwix / 13.1 / -0.6 / -20.3 / 14.2 / -1.7 / -21.4 / 15.8 / -3.4 / -23.0
The Crags, Redford Road / 12.9 / -0.4 / -20.1 / 13.9 / -1.4 / -21.1 / 15.5 / -3.1 / -22.7
Figure S1 Diagram showing setup of Perspex chambers used in assessments of CTs and LTs of C. salviniae (not to scale). A double-jacketed system with eleven separate chambers placed in series is connected to a programmable water bath. Water runs through the system and is returned to the water bath from the outlet of the double-jacketed system, allowing a continuous flow of water which heats or cools the chambers at a set rate of change. Temperature is measured on the bottom of the central chamber using a thermocouple, matching the location of the weevils being tested. The pipes connecting the double-jacketed system to the water bath are insulated with rubber foam to reduce temperature differences between the water within the water bath and that within the double-jacketed system. Replicates are placed into the chambers and the tops of the chambers are covered with a moist cotton ball to prevent desiccation.
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